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The map label for this gene is aspS

Identifier: 20807683

GI number: 20807683

Start: 1232540

End: 1234318

Strand: Direct

Name: aspS

Synonym: TTE1231

Alternate gene names: 20807683

Gene position: 1232540-1234318 (Clockwise)

Preceding gene: 20807682

Following gene: 20807684

Centisome position: 45.83

GC content: 40.58

Gene sequence:

>1779_bases
ATGGGAGAACAACTTGGCGGTCTTAAAAGAACCCATATGTGCGGAGAGCTCGGGGTTAAAGATGTGGGAAAGTCTGTCGT
AGTGATGGGATGGGTCAACAGCAGGAGAGACCACGGAGGATTAGTTTTTATAGATTTGAGGGACAGAACAGGAATTGTGC
AGATAGTATTCAGTGAACAGGTTTCTAAAGAGGTTTTTGAAAAGGTGCAAAGCGTGAGAAGTGAATATGTATTGGCAGTG
GAAGGGGAAGTAGTAAAAAGACTTCCTGAAAATGTAAATCCTAAAATACCCACAGGAGAAATAGAAATTTACGCCAAAAA
TTTGAAGATTTTGAGCAAGTCTGAAACACCTCCTTTTCCAATAGAGGATAGGAGCAATGTATCGGAAGCGGTAAGGCTCA
AATACAGGTACCTGGACTTGAGAAGACCTTCAATGCAGAGGAATTTAATCACTCGCTTTAAGCTTACGCAAGCCGTGAGG
GAGTTTCTCAATGACAATGGCTTTATTGAAATAGAAACTCCCATGCTTATAAAGAGCACCCCGGAAGGCGCCAGGGATTA
TCTTGTGCCAAGCAGAATTTATCCTGGCAAGTTTTACGCGCTGCCTCAGTCTCCCCAGATATTCAAGCAGCTTTTGATGA
TTGCGGGATTTGACAGGTATTATCAAATTGCAAGGTGCTTAAGGGATGAAGACTTGAGAGCTGACAGGCAGCCGGAATTT
ACGCAGATAGATATTGAAATGTCCTTTGTAGAGGTGGAAGATGTCCTTGATATAAATGAGAGAATGATAAAGCATGTATT
CAAAAAGGTTTTGGATGTAGACCTTGATATTCCTTTTAGAAGGTTGACTTATCAGGAAGCAATGGAGAGGTTTGGAACTG
ATAAGCCTGACTTGAGGTTTGGAATGGAGCTTAAAGATTTGTCCGACATACTAAGAGAGTCAGAGTTTAATGTTTTTAAA
AATGCTTTGAAAAATGGCGGTTCAATAAGGGGTATAAATGTAAAAGGAGCTGCTTCAATGACAAGAAAGCAGCTGGATGA
ACTAGTAGAATTTGCCAAAAACTTTGGGGCAAAAGGCCTCTTGTGGATGCAAGTTTTGGAAGGGGAAGTGAAGTCACCTG
CCACTAAATTTTTGACTGAAGGAGAATTGAATAAAATTTTGGAAAGACTTGAAGCAGAAGTGGGAGATTTACTTCTCATA
GTGGCGGATAAAGATGAAGTGGTTTTTGACACTTTAGGGCATTTGAGAGTTGAAATGGCAAAAAGATTTAATCTTATTGA
CGAAAGCAAATATGAGTTTGTATGGGTAGTGGATTTTCCGCTTTTAGAGTATGACGAAGAAGAGAAGAGGTATGTTGCAA
AGCACCATCCATTTACATCACCTAAAGATGAAGATATAGACCTTTTAGAAAAAGAGCCTCTAAAAGTTAGAGCGAAAGCC
TACGATATAGTATTAAACGGCACAGAAATAGGCGGTGGAAGCATAAGAATACATGATACTGAATTGCAGAAGAGGATGTT
TAAAGTGCTGGGATTTAGCGAAGAGGAAGCATGGAAAAAATTTGGATTTTTAATGGAGGCTTTCAAATACGGTGCTCCTC
CTCACGGTGGAATAGCGTATGGGCTTGACAGGCTTGCAATGATAATGACAGGAAGCGACACCATAAGGGATGTGATTGCA
TTTCCCAAAACTCAAAACGCTGTGTGCCTGATGTCAGATGCACCATCGGAAGTGTCTGAAAAGCAGCTTAAGGAACTTCA
CATCAAGATAGACCTATAA

Upstream 100 bases:

>100_bases
GCAAAAAAGTGAAATTGAAGAATATGGCTACAGGAGAAGAGACAGAAATTTTAATTGATGAAATAGAAAAAGCGATAAAA
AATTGAATGGAGGGAGTTTA

Downstream 100 bases:

>100_bases
ACGTTTACCAGTTGAAAAATAGAGGGTTTTATGATATTATTTATATAGGCAAAGGAATAAAGCGAAAAAACCCTGTGGTG
TTCGTGTGACCCGGTGAAAG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MGEQLGGLKRTHMCGELGVKDVGKSVVVMGWVNSRRDHGGLVFIDLRDRTGIVQIVFSEQVSKEVFEKVQSVRSEYVLAV
EGEVVKRLPENVNPKIPTGEIEIYAKNLKILSKSETPPFPIEDRSNVSEAVRLKYRYLDLRRPSMQRNLITRFKLTQAVR
EFLNDNGFIEIETPMLIKSTPEGARDYLVPSRIYPGKFYALPQSPQIFKQLLMIAGFDRYYQIARCLRDEDLRADRQPEF
TQIDIEMSFVEVEDVLDINERMIKHVFKKVLDVDLDIPFRRLTYQEAMERFGTDKPDLRFGMELKDLSDILRESEFNVFK
NALKNGGSIRGINVKGAASMTRKQLDELVEFAKNFGAKGLLWMQVLEGEVKSPATKFLTEGELNKILERLEAEVGDLLLI
VADKDEVVFDTLGHLRVEMAKRFNLIDESKYEFVWVVDFPLLEYDEEEKRYVAKHHPFTSPKDEDIDLLEKEPLKVRAKA
YDIVLNGTEIGGGSIRIHDTELQKRMFKVLGFSEEEAWKKFGFLMEAFKYGAPPHGGIAYGLDRLAMIMTGSDTIRDVIA
FPKTQNAVCLMSDAPSEVSEKQLKELHIKIDL

Sequences:

>Translated_592_residues
MGEQLGGLKRTHMCGELGVKDVGKSVVVMGWVNSRRDHGGLVFIDLRDRTGIVQIVFSEQVSKEVFEKVQSVRSEYVLAV
EGEVVKRLPENVNPKIPTGEIEIYAKNLKILSKSETPPFPIEDRSNVSEAVRLKYRYLDLRRPSMQRNLITRFKLTQAVR
EFLNDNGFIEIETPMLIKSTPEGARDYLVPSRIYPGKFYALPQSPQIFKQLLMIAGFDRYYQIARCLRDEDLRADRQPEF
TQIDIEMSFVEVEDVLDINERMIKHVFKKVLDVDLDIPFRRLTYQEAMERFGTDKPDLRFGMELKDLSDILRESEFNVFK
NALKNGGSIRGINVKGAASMTRKQLDELVEFAKNFGAKGLLWMQVLEGEVKSPATKFLTEGELNKILERLEAEVGDLLLI
VADKDEVVFDTLGHLRVEMAKRFNLIDESKYEFVWVVDFPLLEYDEEEKRYVAKHHPFTSPKDEDIDLLEKEPLKVRAKA
YDIVLNGTEIGGGSIRIHDTELQKRMFKVLGFSEEEAWKKFGFLMEAFKYGAPPHGGIAYGLDRLAMIMTGSDTIRDVIA
FPKTQNAVCLMSDAPSEVSEKQLKELHIKIDL
>Mature_591_residues
GEQLGGLKRTHMCGELGVKDVGKSVVVMGWVNSRRDHGGLVFIDLRDRTGIVQIVFSEQVSKEVFEKVQSVRSEYVLAVE
GEVVKRLPENVNPKIPTGEIEIYAKNLKILSKSETPPFPIEDRSNVSEAVRLKYRYLDLRRPSMQRNLITRFKLTQAVRE
FLNDNGFIEIETPMLIKSTPEGARDYLVPSRIYPGKFYALPQSPQIFKQLLMIAGFDRYYQIARCLRDEDLRADRQPEFT
QIDIEMSFVEVEDVLDINERMIKHVFKKVLDVDLDIPFRRLTYQEAMERFGTDKPDLRFGMELKDLSDILRESEFNVFKN
ALKNGGSIRGINVKGAASMTRKQLDELVEFAKNFGAKGLLWMQVLEGEVKSPATKFLTEGELNKILERLEAEVGDLLLIV
ADKDEVVFDTLGHLRVEMAKRFNLIDESKYEFVWVVDFPLLEYDEEEKRYVAKHHPFTSPKDEDIDLLEKEPLKVRAKAY
DIVLNGTEIGGGSIRIHDTELQKRMFKVLGFSEEEAWKKFGFLMEAFKYGAPPHGGIAYGLDRLAMIMTGSDTIRDVIAF
PKTQNAVCLMSDAPSEVSEKQLKELHIKIDL

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=593, Percent_Identity=43.6762225969646, Blast_Score=471, Evalue=1e-132,
Organism=Homo sapiens, GI5031815, Length=295, Percent_Identity=27.7966101694915, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI194272210, Length=295, Percent_Identity=27.4576271186441, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI45439306, Length=254, Percent_Identity=27.9527559055118, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI226491502, Length=269, Percent_Identity=23.4200743494424, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788173, Length=589, Percent_Identity=50.5942275042445, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1789256, Length=321, Percent_Identity=27.4143302180685, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790571, Length=290, Percent_Identity=27.2413793103448, Blast_Score=104, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32566633, Length=602, Percent_Identity=37.5415282392027, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17551876, Length=276, Percent_Identity=27.1739130434783, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994340, Length=297, Percent_Identity=25.2525252525253, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=297, Percent_Identity=25.2525252525253, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=297, Percent_Identity=25.2525252525253, Blast_Score=86, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325153, Length=638, Percent_Identity=35.1097178683386, Blast_Score=301, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6323011, Length=352, Percent_Identity=28.9772727272727, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320242, Length=289, Percent_Identity=24.2214532871972, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=285, Percent_Identity=24.2105263157895, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24584738, Length=597, Percent_Identity=38.0234505862647, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17136276, Length=273, Percent_Identity=27.8388278388278, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24640849, Length=379, Percent_Identity=24.802110817942, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24640851, Length=379, Percent_Identity=24.802110817942, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19921528, Length=294, Percent_Identity=26.530612244898, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_THETN (Q8RAI7)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622854.1
- ProteinModelPortal:   Q8RAI7
- GeneID:   997516
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1231
- NMPDR:   fig|273068.3.peg.1021
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   TTEN273068:TTE1231-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67865; Mature: 67734

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEQLGGLKRTHMCGELGVKDVGKSVVVMGWVNSRRDHGGLVFIDLRDRTGIVQIVFSEQ
CCCCCCCCHHHHHHHHCCHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEHHH
VSKEVFEKVQSVRSEYVLAVEGEVVKRLPENVNPKIPTGEIEIYAKNLKILSKSETPPFP
HHHHHHHHHHHHHHCEEEEECCHHHHHCCCCCCCCCCCCCEEEEEECEEEEECCCCCCCC
IEDRSNVSEAVRLKYRYLDLRRPSMQRNLITRFKLTQAVREFLNDNGFIEIETPMLIKST
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECC
PEGARDYLVPSRIYPGKFYALPQSPQIFKQLLMIAGFDRYYQIARCLRDEDLRADRQPEF
CCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
TQIDIEMSFVEVEDVLDINERMIKHVFKKVLDVDLDIPFRRLTYQEAMERFGTDKPDLRF
EEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
GMELKDLSDILRESEFNVFKNALKNGGSIRGINVKGAASMTRKQLDELVEFAKNFGAKGL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCH
LWMQVLEGEVKSPATKFLTEGELNKILERLEAEVGDLLLIVADKDEVVFDTLGHLRVEMA
HHHHHHHCHHCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHH
KRFNLIDESKYEFVWVVDFPLLEYDEEEKRYVAKHHPFTSPKDEDIDLLEKEPLKVRAKA
HHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHCCCCCEEEEEE
YDIVLNGTEIGGGSIRIHDTELQKRMFKVLGFSEEEAWKKFGFLMEAFKYGAPPHGGIAY
EEEEEECCEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
GLDRLAMIMTGSDTIRDVIAFPKTQNAVCLMSDAPSEVSEKQLKELHIKIDL
HHHHHEEEEECCHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHEEEEEEC
>Mature Secondary Structure 
GEQLGGLKRTHMCGELGVKDVGKSVVVMGWVNSRRDHGGLVFIDLRDRTGIVQIVFSEQ
CCCCCCCHHHHHHHHCCHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEHHH
VSKEVFEKVQSVRSEYVLAVEGEVVKRLPENVNPKIPTGEIEIYAKNLKILSKSETPPFP
HHHHHHHHHHHHHHCEEEEECCHHHHHCCCCCCCCCCCCCEEEEEECEEEEECCCCCCCC
IEDRSNVSEAVRLKYRYLDLRRPSMQRNLITRFKLTQAVREFLNDNGFIEIETPMLIKST
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECC
PEGARDYLVPSRIYPGKFYALPQSPQIFKQLLMIAGFDRYYQIARCLRDEDLRADRQPEF
CCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
TQIDIEMSFVEVEDVLDINERMIKHVFKKVLDVDLDIPFRRLTYQEAMERFGTDKPDLRF
EEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
GMELKDLSDILRESEFNVFKNALKNGGSIRGINVKGAASMTRKQLDELVEFAKNFGAKGL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCH
LWMQVLEGEVKSPATKFLTEGELNKILERLEAEVGDLLLIVADKDEVVFDTLGHLRVEMA
HHHHHHHCHHCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHH
KRFNLIDESKYEFVWVVDFPLLEYDEEEKRYVAKHHPFTSPKDEDIDLLEKEPLKVRAKA
HHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHCCCCCEEEEEE
YDIVLNGTEIGGGSIRIHDTELQKRMFKVLGFSEEEAWKKFGFLMEAFKYGAPPHGGIAY
EEEEEECCEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
GLDRLAMIMTGSDTIRDVIAFPKTQNAVCLMSDAPSEVSEKQLKELHIKIDL
HHHHHEEEEECCHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336