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The map label for this gene is GloA2 [H]

Identifier: 20807674

GI number: 20807674

Start: 1221213

End: 1221614

Strand: Direct

Name: GloA2 [H]

Synonym: TTE1219

Alternate gene names: 20807674

Gene position: 1221213-1221614 (Clockwise)

Preceding gene: 20807673

Following gene: 20807675

Centisome position: 45.41

GC content: 43.28

Gene sequence:

>402_bases
GTGATTAAAAAGGTAGACCACATAGGGATAGCAGTAAAAAGCATAGAAGAAGCGAGAAAGTTTTATGAAGAAGTACTGGG
CTTGAAAGTGACAGGGATAGAAGAAGTTAAGGAGCAGAAGGTAAAGACGGCCTTTATTCCAGTTGGGGACAGCGAGATAG
AGCTTTTAGAGTCCACATCAGAGGATGGGCCTGTCGCTAAATTTATAGAAAAAAGAGGGGAAGGCATACACCACATAGCA
CTTCAGGTAGATGACATTGAAAAGGCGTTAGAGGAGTTGAAGGGAAAAGGAGTAAAGCTTATAGATGAAGTGCCAAGGTA
TGGGGCAGGAGGGGCGAAAATTGCCTTTGTCCACCCAAAGAACGCAAATGGAGTATTGTTAGAGCTTTGCGAGAGAGATT
AA

Upstream 100 bases:

>100_bases
CTACGCTGAAGACGATAAAGAAAAAATGCTGGTAAGAAGATTGACCTTTATCCAGTAACAGGAGTTCTCTATAAGCAAAT
ACTTCAGAGGAGGGAATGAC

Downstream 100 bases:

>100_bases
AACCACAACGTTCCTGGAGGTATGATAGAATGGATGTTCAGGCAAAAATAGAGGAACTAAAAAGAAGGTATGAAAAAATA
AAAGAAGGGGGAGGTCAGAA

Product: lactoylglutathione lyase and related lyase

Products: (R)-S-Lactoylglutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MIKKVDHIGIAVKSIEEARKFYEEVLGLKVTGIEEVKEQKVKTAFIPVGDSEIELLESTSEDGPVAKFIEKRGEGIHHIA
LQVDDIEKALEELKGKGVKLIDEVPRYGAGGAKIAFVHPKNANGVLLELCERD

Sequences:

>Translated_133_residues
MIKKVDHIGIAVKSIEEARKFYEEVLGLKVTGIEEVKEQKVKTAFIPVGDSEIELLESTSEDGPVAKFIEKRGEGIHHIA
LQVDDIEKALEELKGKGVKLIDEVPRYGAGGAKIAFVHPKNANGVLLELCERD
>Mature_133_residues
MIKKVDHIGIAVKSIEEARKFYEEVLGLKVTGIEEVKEQKVKTAFIPVGDSEIELLESTSEDGPVAKFIEKRGEGIHHIA
LQVDDIEKALEELKGKGVKLIDEVPRYGAGGAKIAFVHPKNANGVLLELCERD

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI188035928, Length=127, Percent_Identity=37.007874015748, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17506387, Length=130, Percent_Identity=36.9230769230769, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR018146
- InterPro:   IPR017515 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5 [C]

Molecular weight: Translated: 14632; Mature: 14632

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKVDHIGIAVKSIEEARKFYEEVLGLKVTGIEEVKEQKVKTAFIPVGDSEIELLESTS
CCCCCHHHHHHHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHEEEEECCCCHHHHHHCCC
EDGPVAKFIEKRGEGIHHIALQVDDIEKALEELKGKGVKLIDEVPRYGAGGAKIAFVHPK
CCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEEECCC
NANGVLLELCERD
CCCCCEEEEECCC
>Mature Secondary Structure
MIKKVDHIGIAVKSIEEARKFYEEVLGLKVTGIEEVKEQKVKTAFIPVGDSEIELLESTS
CCCCCHHHHHHHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHEEEEECCCCHHHHHHCCC
EDGPVAKFIEKRGEGIHHIALQVDDIEKALEELKGKGVKLIDEVPRYGAGGAKIAFVHPK
CCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEEECCC
NANGVLLELCERD
CCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: Reduced glutathione; Methylglyoxal [C]

Specific reaction: Reduced glutathione + Methylglyoxal --> (R)-S-Lactoylglutathione [C]

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]