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The map label for this gene is queA

Identifier: 20807638

GI number: 20807638

Start: 1179566

End: 1180591

Strand: Direct

Name: queA

Synonym: TTE1182

Alternate gene names: 20807638

Gene position: 1179566-1180591 (Clockwise)

Preceding gene: 20807637

Following gene: 20807639

Centisome position: 43.86

GC content: 41.23

Gene sequence:

>1026_bases
ATGAAGCGAAGTGAATTCTACTTTGATTTGCCGGAGGAGTTAATTGCTCAAGAGCCATTAGAAGATAGGGCAAGTTCCAG
ATTGATGATTTTAGATAGAAGGACAGGGGAAATAAAGCACGACATTTTTAAAAACATAACTAAATATCTAAAAGAAGGGG
ATTGTCTGGTTTTAAATGACACAAAAGTAATTCCTGCAAGGCTCATTGGACAGAGGGAGGATTCTGGAGGAAAGGTAGAG
CTTTTGCTTTTGAGAAGGACTTCAATGAATGAGTGGGAGGTTTTAGTGAAACCAGGCAAAAGGGCAAAGGTGGGCAAAAG
GGTGGTATTTGGAAATGGAGAGCTTGTGGCAGAAATAATCGATACCACAGAGGCGGGAGGGCGCATAGCAAGATTTTACT
ACGATGGGGTGTTCGAAGAGGTATTGGACAGGTTGGGGGAGATGCCTGTTCCTCCTTATATAAAGAAAAAGCTGAAGGAC
AAGAATAGGTATCAAACGGTATACGCCAAGTATGAAGGCTCGGCTGCAGCACCTACAGCAGGTCTTCATTTCACAGAGGA
ATTGCTGGATGAAATTCGAAATATGGGAGTAAAGACGGTTTTCATAACTCTTCACGTGGGGTTGGGCACTTTCAGGCCTG
TTAAAGAGGAAATAATAGAGAACCACAAGATGCACGAGGAGTTTTACATTGTTACAGAAGAAGCCGCAAAAGCCATAAAT
GAAGCTCGAAAAAATGGAGGCCGCATAATAGCTGTAGGGACTACTTCTACTCGCACTTTAGAGACGGTTGCAGATGAGAG
CGGATATATTCATCCAAAAAGCGGATGGACAGATATTTTTATATATCCTGGATATAAGTTTAAAGCGATAGACGGGATGA
TTACAAATTTCCATCTGCCTGAATCTACCCTCATAATGATGGTTTCTGCTTTTGCAGGAAAAGAGAATATAATGAGGGCG
TATAAAGTGGCTATTGAGAATAAATACAGGTTCTTTAGCTTTGGAGATGCAATGCTTATCATATAA

Upstream 100 bases:

>100_bases
TAACACTTCTTTTCTGGCTTTTTAGAAGGTAAGTATTGAAAATTTTTTGATTTAACGGTATAATCACCATATGCATTCTT
GAAAAGGGATGGAGTAGGGT

Downstream 100 bases:

>100_bases
AGGACAGGAGGTAAAAATGGCTGCGATAAAGTACCAAGTTATAAAAAAAGATGCAAGGACCAAGGCAAGATTGGGAATTC
TTGAAACCCCCCATGGAGTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKRSEFYFDLPEELIAQEPLEDRASSRLMILDRRTGEIKHDIFKNITKYLKEGDCLVLNDTKVIPARLIGQREDSGGKVE
LLLLRRTSMNEWEVLVKPGKRAKVGKRVVFGNGELVAEIIDTTEAGGRIARFYYDGVFEEVLDRLGEMPVPPYIKKKLKD
KNRYQTVYAKYEGSAAAPTAGLHFTEELLDEIRNMGVKTVFITLHVGLGTFRPVKEEIIENHKMHEEFYIVTEEAAKAIN
EARKNGGRIIAVGTTSTRTLETVADESGYIHPKSGWTDIFIYPGYKFKAIDGMITNFHLPESTLIMMVSAFAGKENIMRA
YKVAIENKYRFFSFGDAMLII

Sequences:

>Translated_341_residues
MKRSEFYFDLPEELIAQEPLEDRASSRLMILDRRTGEIKHDIFKNITKYLKEGDCLVLNDTKVIPARLIGQREDSGGKVE
LLLLRRTSMNEWEVLVKPGKRAKVGKRVVFGNGELVAEIIDTTEAGGRIARFYYDGVFEEVLDRLGEMPVPPYIKKKLKD
KNRYQTVYAKYEGSAAAPTAGLHFTEELLDEIRNMGVKTVFITLHVGLGTFRPVKEEIIENHKMHEEFYIVTEEAAKAIN
EARKNGGRIIAVGTTSTRTLETVADESGYIHPKSGWTDIFIYPGYKFKAIDGMITNFHLPESTLIMMVSAFAGKENIMRA
YKVAIENKYRFFSFGDAMLII
>Mature_341_residues
MKRSEFYFDLPEELIAQEPLEDRASSRLMILDRRTGEIKHDIFKNITKYLKEGDCLVLNDTKVIPARLIGQREDSGGKVE
LLLLRRTSMNEWEVLVKPGKRAKVGKRVVFGNGELVAEIIDTTEAGGRIARFYYDGVFEEVLDRLGEMPVPPYIKKKLKD
KNRYQTVYAKYEGSAAAPTAGLHFTEELLDEIRNMGVKTVFITLHVGLGTFRPVKEEIIENHKMHEEFYIVTEEAAKAIN
EARKNGGRIIAVGTTSTRTLETVADESGYIHPKSGWTDIFIYPGYKFKAIDGMITNFHLPESTLIMMVSAFAGKENIMRA
YKVAIENKYRFFSFGDAMLII

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=45.9302325581395, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_THETN (Q8RAN0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622809.1
- ProteinModelPortal:   Q8RAN0
- SMR:   Q8RAN0
- GeneID:   995520
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1182
- NMPDR:   fig|273068.3.peg.879
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- ProtClustDB:   PRK00147
- BioCyc:   TTEN273068:TTE1182-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38791; Mature: 38791

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSEFYFDLPEELIAQEPLEDRASSRLMILDRRTGEIKHDIFKNITKYLKEGDCLVLND
CCCCCEEECCCHHHHHCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEC
TKVIPARLIGQREDSGGKVELLLLRRTSMNEWEVLVKPGKRAKVGKRVVFGNGELVAEII
CCCCHHHHCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCEEEECCCCEEEEEH
DTTEAGGRIARFYYDGVFEEVLDRLGEMPVPPYIKKKLKDKNRYQTVYAKYEGSAAAPTA
HCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCC
GLHFTEELLDEIRNMGVKTVFITLHVGLGTFRPVKEEIIENHKMHEEFYIVTEEAAKAIN
CCHHHHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHH
EARKNGGRIIAVGTTSTRTLETVADESGYIHPKSGWTDIFIYPGYKFKAIDGMITNFHLP
HHHHCCCEEEEEECCCCCHHHHHHCCCCEECCCCCCEEEEEECCEEEEEECCEEEEECCC
ESTLIMMVSAFAGKENIMRAYKVAIENKYRFFSFGDAMLII
CHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCEEEEC
>Mature Secondary Structure
MKRSEFYFDLPEELIAQEPLEDRASSRLMILDRRTGEIKHDIFKNITKYLKEGDCLVLND
CCCCCEEECCCHHHHHCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEC
TKVIPARLIGQREDSGGKVELLLLRRTSMNEWEVLVKPGKRAKVGKRVVFGNGELVAEII
CCCCHHHHCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCEEEECCCCEEEEEH
DTTEAGGRIARFYYDGVFEEVLDRLGEMPVPPYIKKKLKDKNRYQTVYAKYEGSAAAPTA
HCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCC
GLHFTEELLDEIRNMGVKTVFITLHVGLGTFRPVKEEIIENHKMHEEFYIVTEEAAKAIN
CCHHHHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHH
EARKNGGRIIAVGTTSTRTLETVADESGYIHPKSGWTDIFIYPGYKFKAIDGMITNFHLP
HHHHCCCEEEEEECCCCCHHHHHHCCCCEECCCCCCEEEEEECCEEEEEECCEEEEECCC
ESTLIMMVSAFAGKENIMRAYKVAIENKYRFFSFGDAMLII
CHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336