Click here to switch to the map view.

The map label for this gene is tgt

Identifier: 20807639

GI number: 20807639

Start: 1180608

End: 1181735

Strand: Direct

Name: tgt

Synonym: TTE1183

Alternate gene names: 20807639

Gene position: 1180608-1181735 (Clockwise)

Preceding gene: 20807638

Following gene: 20807640

Centisome position: 43.9

GC content: 40.43

Gene sequence:

>1128_bases
ATGGCTGCGATAAAGTACCAAGTTATAAAAAAAGATGCAAGGACCAAGGCAAGATTGGGAATTCTTGAAACCCCCCATGG
AGTTATAGAGACGCCAGTTTTTATGCCTGTAGGTACGCAGGCTACAGTGAAAGCGATGACCCCTGATGAGCTAAAAGAAA
TGGGCGCTACAATTATTTTAGGAAACACCTACCATCTTTATTTAAGGCCAGGCCACAAGATTATTGAAAAAGCAGGTGGT
CTTCACAAATTCATGAACTGGGATAGGGCTATTTTAACAGATAGCGGCGGATTTCAAGTTTTTAGTTTAAGCTCTTTGAG
AAAAATTACAGAAGATGGCGTGGAATTTAGGTCTCACATTGACGGGTCAAAACATTTTTTTACTCCTGAAAAAGTGATAG
AGATACAGAATTCGCTGGGGTCTGATATAATCATGTCTTTTGATGAATGTGCCCCTTATCCTGCTGACTATGACTATGTA
AAAAGGTCTATGGAACTTACAATAAAGTGGGCGAAGAGGGGGAAAAAAGCCCATAAAAATACGGATAGACAGGCTCTTTT
TGGCATTGTGCAAGGGGGAACTTATAAAGATTTAAGAAGAGAGTGCGCAGAAAGGTTAGTTGATATGGATTTTCCCGGAT
ATGCTATTGGGGGTTTAAGTGTTGGTGAGCCAAAAGACCTTATGTACGAAATTATAGATTTTACTACTGATTATTTGCCT
CACGATAAACCGAGATATCTGATGGGGGTGGGAACGCCCGAAGACCTTATAGAAGGTGTGATAAGAGGGGTTGACATGTT
TGACTGTGTATTGCCTACGCGAATTGCCAGAAATGGAACTGTATTTACAAGCAGAGGCAAATTGATAGTGAGAGATGCGC
CGTATGCTGAAGACTTTTCGCCACTGGATGAGGAATGCGACTGTTACACCTGCAAGAATTATTCTAGAGCTTATTTAAGG
CATCTTTTTAAGGCGAAGGAAATTTTGGCGGCGAGGCTTGCTACCTACCACAATCTCTATTTTTTAATTAAATTGATGGA
GAAAATAAGGGAGGCCATAAGGCAAGATCGCCTGCTGGAATTTAAAGAAGAGTTTTTAAAAAAATATTATGGGAACAGGG
AGGAATAG

Upstream 100 bases:

>100_bases
GAGAATATAATGAGGGCGTATAAAGTGGCTATTGAGAATAAATACAGGTTCTTTAGCTTTGGAGATGCAATGCTTATCAT
ATAAAGGACAGGAGGTAAAA

Downstream 100 bases:

>100_bases
TGAATGAACGAAACTACTTATGTACTCATTCAATTTGCGATTTTAATTGCAATTTTTTACTTTTTACTTATTTTGCCACA
GCAGAGAAGGCAGAAAAAAG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MAAIKYQVIKKDARTKARLGILETPHGVIETPVFMPVGTQATVKAMTPDELKEMGATIILGNTYHLYLRPGHKIIEKAGG
LHKFMNWDRAILTDSGGFQVFSLSSLRKITEDGVEFRSHIDGSKHFFTPEKVIEIQNSLGSDIIMSFDECAPYPADYDYV
KRSMELTIKWAKRGKKAHKNTDRQALFGIVQGGTYKDLRRECAERLVDMDFPGYAIGGLSVGEPKDLMYEIIDFTTDYLP
HDKPRYLMGVGTPEDLIEGVIRGVDMFDCVLPTRIARNGTVFTSRGKLIVRDAPYAEDFSPLDEECDCYTCKNYSRAYLR
HLFKAKEILAARLATYHNLYFLIKLMEKIREAIRQDRLLEFKEEFLKKYYGNREE

Sequences:

>Translated_375_residues
MAAIKYQVIKKDARTKARLGILETPHGVIETPVFMPVGTQATVKAMTPDELKEMGATIILGNTYHLYLRPGHKIIEKAGG
LHKFMNWDRAILTDSGGFQVFSLSSLRKITEDGVEFRSHIDGSKHFFTPEKVIEIQNSLGSDIIMSFDECAPYPADYDYV
KRSMELTIKWAKRGKKAHKNTDRQALFGIVQGGTYKDLRRECAERLVDMDFPGYAIGGLSVGEPKDLMYEIIDFTTDYLP
HDKPRYLMGVGTPEDLIEGVIRGVDMFDCVLPTRIARNGTVFTSRGKLIVRDAPYAEDFSPLDEECDCYTCKNYSRAYLR
HLFKAKEILAARLATYHNLYFLIKLMEKIREAIRQDRLLEFKEEFLKKYYGNREE
>Mature_374_residues
AAIKYQVIKKDARTKARLGILETPHGVIETPVFMPVGTQATVKAMTPDELKEMGATIILGNTYHLYLRPGHKIIEKAGGL
HKFMNWDRAILTDSGGFQVFSLSSLRKITEDGVEFRSHIDGSKHFFTPEKVIEIQNSLGSDIIMSFDECAPYPADYDYVK
RSMELTIKWAKRGKKAHKNTDRQALFGIVQGGTYKDLRRECAERLVDMDFPGYAIGGLSVGEPKDLMYEIIDFTTDYLPH
DKPRYLMGVGTPEDLIEGVIRGVDMFDCVLPTRIARNGTVFTSRGKLIVRDAPYAEDFSPLDEECDCYTCKNYSRAYLRH
LFKAKEILAARLATYHNLYFLIKLMEKIREAIRQDRLLEFKEEFLKKYYGNREE

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=366, Percent_Identity=46.9945355191257, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI13375872, Length=262, Percent_Identity=26.3358778625954, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1786607, Length=366, Percent_Identity=56.5573770491803, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17544680, Length=356, Percent_Identity=48.0337078651685, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI25146502, Length=185, Percent_Identity=31.3513513513514, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=46.866485013624, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21355311, Length=166, Percent_Identity=33.1325301204819, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_THETN (Q8RAM9)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622810.1
- ProteinModelPortal:   Q8RAM9
- SMR:   Q8RAM9
- GeneID:   995518
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1183
- NMPDR:   fig|273068.3.peg.926
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   TTEN273068:TTE1183-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42931; Mature: 42800

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS01109 RIBOSOMAL_L10

Important sites: ACT_SITE 94-94 BINDING 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIKYQVIKKDARTKARLGILETPHGVIETPVFMPVGTQATVKAMTPDELKEMGATIIL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHCCCEEEE
GNTYHLYLRPGHKIIEKAGGLHKFMNWDRAILTDSGGFQVFSLSSLRKITEDGVEFRSHI
CCEEEEEECCCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHC
DGSKHFFTPEKVIEIQNSLGSDIIMSFDECAPYPADYDYVKRSMELTIKWAKRGKKAHKN
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCC
TDRQALFGIVQGGTYKDLRRECAERLVDMDFPGYAIGGLSVGEPKDLMYEIIDFTTDYLP
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCC
HDKPRYLMGVGTPEDLIEGVIRGVDMFDCVLPTRIARNGTVFTSRGKLIVRDAPYAEDFS
CCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCC
PLDEECDCYTCKNYSRAYLRHLFKAKEILAARLATYHNLYFLIKLMEKIREAIRQDRLLE
CCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKEEFLKKYYGNREE
HHHHHHHHHCCCCCC
>Mature Secondary Structure 
AAIKYQVIKKDARTKARLGILETPHGVIETPVFMPVGTQATVKAMTPDELKEMGATIIL
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHCCCEEEE
GNTYHLYLRPGHKIIEKAGGLHKFMNWDRAILTDSGGFQVFSLSSLRKITEDGVEFRSHI
CCEEEEEECCCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHC
DGSKHFFTPEKVIEIQNSLGSDIIMSFDECAPYPADYDYVKRSMELTIKWAKRGKKAHKN
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCC
TDRQALFGIVQGGTYKDLRRECAERLVDMDFPGYAIGGLSVGEPKDLMYEIIDFTTDYLP
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCC
HDKPRYLMGVGTPEDLIEGVIRGVDMFDCVLPTRIARNGTVFTSRGKLIVRDAPYAEDFS
CCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCC
PLDEECDCYTCKNYSRAYLRHLFKAKEILAARLATYHNLYFLIKLMEKIREAIRQDRLLE
CCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKEEFLKKYYGNREE
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336