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The map label for this gene is AnsB [H]

Identifier: 20807447

GI number: 20807447

Start: 969777

End: 970754

Strand: Reverse

Name: AnsB [H]

Synonym: TTE0966

Alternate gene names: 20807447

Gene position: 970754-969777 (Counterclockwise)

Preceding gene: 20807466

Following gene: 20807442

Centisome position: 36.09

GC content: 39.57

Gene sequence:

>978_bases
TTGGGTAAAGTGGCTGTGATTTTTACAGGTGGAACCATAGCGATGAAGCAAGACCCCAATCTGAAAGGGGCAGTCCCTTC
CCTCACGGGAAAAGACCTGCTTGAAATTATTCCTCACAGAGAAAACGTTTTAGAAATTGAAACTATAGAATTTGGAAACT
ACCCTAGTCCTCATCTCACAGTAGAACTTTTGGAAGAATTAAGAGAAGTCGTAGAGAAGACCCTTAAAAGAGATGAAATA
AAAGGAGTTGTGATAACCCACGGCACTGATACATTAGAAGAAACAGCTTATTTTTTAGAGCTCACCACAAATTTTGACAA
ACCTATTGTCTTAACTGGCGCAATGAAAAATTCCTCTGAAGCAGGTTATGATGGGCCTTCCAATTTAATTGACTCTCTCA
CCACAGCCTCTTCAGAAAAATCCAAAAAAAGAGGCGTGTTAGTAGTGCTAAACGGTGAAATTCATTCTGCAAAAGATGTA
ACGAAAACCCATACTTCAAGTCTTGACACTTTTAAAAGCCCTGAAACAGGTCCTCTGGGGTATGTGGACAGTGGCAATGT
GTATTTTTATAGAAACGTAGAAAAAAGGCAGTACATTCCCACAAAAAAACTGGAACGTCGCGTAAGCCTTTTAAAAGTAG
TGTTTGGAATGGATGACAAACTGATAAGATTTTTAGTAGAAAATGGAGAAAGAGGCATTGTCATTGAAGGGACGGGCAGA
GGAAATGTGCCTCCAAAAGTAGCAGAAGGAATAAAATACGCTATATCTAAAGGAACAGTTGTAGTTCTCGTATCAAGGTG
CCCGATGGGAAGAGTACATCCTTCCTATGCTTATGAAGGAGGAGGAAAACACCTCGAATCTCTGGGGGTAATTTTTGGAG
GAAATCTATCAGGTCAAAAGGCAAGAGTAAAATTAATGGCAGCACTGGCATACACATCAGATTACAGAAAAATTAGAGAG
ATTTTTGAGGAAGAGTAA

Upstream 100 bases:

>100_bases
ACAAAAAATTCTCTTATGCTTTTAAGACAAGTTTCGCGTATTTGTGCAAAAGCAAAAAATATACTATAATGGAGATAGAA
AAAAACTGGGGGGATGAAAT

Downstream 100 bases:

>100_bases
AAAACAAAAAGGAGAGGTGCTCTCTCCTTTCAGACTGTTGACAAAGTTTAAAAGTGGCATTTTGTGTGTAAATGCGACTT
GTTGTCAAAGCGGTAACTAA

Product: L-asparaginase/ Glu-tRNAGln amidotransferase subunit D

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MGKVAVIFTGGTIAMKQDPNLKGAVPSLTGKDLLEIIPHRENVLEIETIEFGNYPSPHLTVELLEELREVVEKTLKRDEI
KGVVITHGTDTLEETAYFLELTTNFDKPIVLTGAMKNSSEAGYDGPSNLIDSLTTASSEKSKKRGVLVVLNGEIHSAKDV
TKTHTSSLDTFKSPETGPLGYVDSGNVYFYRNVEKRQYIPTKKLERRVSLLKVVFGMDDKLIRFLVENGERGIVIEGTGR
GNVPPKVAEGIKYAISKGTVVVLVSRCPMGRVHPSYAYEGGGKHLESLGVIFGGNLSGQKARVKLMAALAYTSDYRKIRE
IFEEE

Sequences:

>Translated_325_residues
MGKVAVIFTGGTIAMKQDPNLKGAVPSLTGKDLLEIIPHRENVLEIETIEFGNYPSPHLTVELLEELREVVEKTLKRDEI
KGVVITHGTDTLEETAYFLELTTNFDKPIVLTGAMKNSSEAGYDGPSNLIDSLTTASSEKSKKRGVLVVLNGEIHSAKDV
TKTHTSSLDTFKSPETGPLGYVDSGNVYFYRNVEKRQYIPTKKLERRVSLLKVVFGMDDKLIRFLVENGERGIVIEGTGR
GNVPPKVAEGIKYAISKGTVVVLVSRCPMGRVHPSYAYEGGGKHLESLGVIFGGNLSGQKARVKLMAALAYTSDYRKIRE
IFEEE
>Mature_324_residues
GKVAVIFTGGTIAMKQDPNLKGAVPSLTGKDLLEIIPHRENVLEIETIEFGNYPSPHLTVELLEELREVVEKTLKRDEIK
GVVITHGTDTLEETAYFLELTTNFDKPIVLTGAMKNSSEAGYDGPSNLIDSLTTASSEKSKKRGVLVVLNGEIHSAKDVT
KTHTSSLDTFKSPETGPLGYVDSGNVYFYRNVEKRQYIPTKKLERRVSLLKVVFGMDDKLIRFLVENGERGIVIEGTGRG
NVPPKVAEGIKYAISKGTVVVLVSRCPMGRVHPSYAYEGGGKHLESLGVIFGGNLSGQKARVKLMAALAYTSDYRKIREI
FEEE

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=216, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789327, Length=300, Percent_Identity=35, Blast_Score=170, Evalue=9e-44,
Organism=Escherichia coli, GI1788065, Length=339, Percent_Identity=25.6637168141593, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI193207372, Length=341, Percent_Identity=28.4457478005865, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17558280, Length=342, Percent_Identity=28.3625730994152, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17558278, Length=342, Percent_Identity=28.3625730994152, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI193207374, Length=342, Percent_Identity=28.3625730994152, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323189, Length=337, Percent_Identity=32.9376854599407, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323187, Length=337, Percent_Identity=32.9376854599407, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323186, Length=337, Percent_Identity=32.9376854599407, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323184, Length=337, Percent_Identity=32.9376854599407, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320527, Length=333, Percent_Identity=32.4324324324324, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24645530, Length=300, Percent_Identity=26.3333333333333, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24639609, Length=232, Percent_Identity=26.7241379310345, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35732; Mature: 35601

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVAVIFTGGTIAMKQDPNLKGAVPSLTGKDLLEIIPHRENVLEIETIEFGNYPSPHLT
CCCEEEEEECCEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCCCHH
VELLEELREVVEKTLKRDEIKGVVITHGTDTLEETAYFLELTTNFDKPIVLTGAMKNSSE
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHEEEEEEECCCCCCEEEEECCCCCCC
AGYDGPSNLIDSLTTASSEKSKKRGVLVVLNGEIHSAKDVTKTHTSSLDTFKSPETGPLG
CCCCCHHHHHHHHHHCCCCHHHHCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
YVDSGNVYFYRNVEKRQYIPTKKLERRVSLLKVVFGMDDKLIRFLVENGERGIVIEGTGR
EEECCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCC
GNVPPKVAEGIKYAISKGTVVVLVSRCPMGRVHPSYAYEGGGKHLESLGVIFGGNLSGQK
CCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEECCCCHHHHHCCEEECCCCCCCH
ARVKLMAALAYTSDYRKIREIFEEE
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKVAVIFTGGTIAMKQDPNLKGAVPSLTGKDLLEIIPHRENVLEIETIEFGNYPSPHLT
CCEEEEEECCEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCCCHH
VELLEELREVVEKTLKRDEIKGVVITHGTDTLEETAYFLELTTNFDKPIVLTGAMKNSSE
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHEEEEEEECCCCCCEEEEECCCCCCC
AGYDGPSNLIDSLTTASSEKSKKRGVLVVLNGEIHSAKDVTKTHTSSLDTFKSPETGPLG
CCCCCHHHHHHHHHHCCCCHHHHCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
YVDSGNVYFYRNVEKRQYIPTKKLERRVSLLKVVFGMDDKLIRFLVENGERGIVIEGTGR
EEECCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCC
GNVPPKVAEGIKYAISKGTVVVLVSRCPMGRVHPSYAYEGGGKHLESLGVIFGGNLSGQK
CCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEECCCCHHHHHCCEEECCCCCCCH
ARVKLMAALAYTSDYRKIREIFEEE
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]