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The map label for this gene is ThiJ [H]

Identifier: 20807466

GI number: 20807466

Start: 990121

End: 990627

Strand: Reverse

Name: ThiJ [H]

Synonym: TTE0986

Alternate gene names: 20807466

Gene position: 990627-990121 (Counterclockwise)

Preceding gene: 20807467

Following gene: 20807447

Centisome position: 36.83

GC content: 37.48

Gene sequence:

>507_bases
ATGAAAAGAGTAGCCATTTTAATTGAAAACCTCTATGAAGAGCCCGAGCTTTTATATCCATACTACAGATTGAAAGAAGA
AGGGTATGAAGTTCACCTAGTGGGGACAGAAAAAGATAAAGTGTACACAGGCAAATATGGTTATCCTATGAAAAGCACTC
ACGCATCAAAAGAAGTAAAAGCCGATGATTATGATGCTGTGGTAATACCAGGAGGTTATTCTCCGGACCACATGAGAAGG
TGCCAGGATACTGTAAATTTCGTTAAAGAGATGTGCCAACAGCAAAAAATAATTGCTGCGATATGCCATGGACCCTGGAT
GATGGCTTCAAGCTGCGACTTAAAAGGTAAAAGAGTCACAAGCTTTTTCTCAATAAAGGATGACTTAATAAATGCAGGAG
CTCAGTACGTAGACGAAGAAGTAGTAATAGATGGAAATCTAATAACCTCTAGAACTCCCAATGATTTAGTAGCTTTTGTA
AAAGCTATAATTGAAAAACTGAAATAA

Upstream 100 bases:

>100_bases
CAAGAAATAGATTTAGATGTATTTAGCCATTAATGGGATTGACATAAGTATCCAATAAGGCTAAAATAAAGACAAACCAT
GAACAAACGAGGTGGTTTAA

Downstream 100 bases:

>100_bases
GAATACCAAAAAAGGGTTCTGGTACTTTATATGTACCGAGAACCCTTGATTTTTCTGGTGGAGCTGATGGGACTTGAACC
CACGGCCTCCACAATGCCAT

Product: putative intracellular protease/amidase

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MKRVAILIENLYEEPELLYPYYRLKEEGYEVHLVGTEKDKVYTGKYGYPMKSTHASKEVKADDYDAVVIPGGYSPDHMRR
CQDTVNFVKEMCQQQKIIAAICHGPWMMASSCDLKGKRVTSFFSIKDDLINAGAQYVDEEVVIDGNLITSRTPNDLVAFV
KAIIEKLK

Sequences:

>Translated_168_residues
MKRVAILIENLYEEPELLYPYYRLKEEGYEVHLVGTEKDKVYTGKYGYPMKSTHASKEVKADDYDAVVIPGGYSPDHMRR
CQDTVNFVKEMCQQQKIIAAICHGPWMMASSCDLKGKRVTSFFSIKDDLINAGAQYVDEEVVIDGNLITSRTPNDLVAFV
KAIIEKLK
>Mature_168_residues
MKRVAILIENLYEEPELLYPYYRLKEEGYEVHLVGTEKDKVYTGKYGYPMKSTHASKEVKADDYDAVVIPGGYSPDHMRR
CQDTVNFVKEMCQQQKIIAAICHGPWMMASSCDLKGKRVTSFFSIKDDLINAGAQYVDEEVVIDGNLITSRTPNDLVAFV
KAIIEKLK

Specific function: Unknown

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=168, Percent_Identity=35.7142857142857, Blast_Score=115, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17558714, Length=156, Percent_Identity=29.4871794871795, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28571932, Length=168, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 19121; Mature: 19121

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVAILIENLYEEPELLYPYYRLKEEGYEVHLVGTEKDKVYTGKYGYPMKSTHASKEVK
CCHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCC
ADDYDAVVIPGGYSPDHMRRCQDTVNFVKEMCQQQKIIAAICHGPWMMASSCDLKGKRVT
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHH
SFFSIKDDLINAGAQYVDEEVVIDGNLITSRTPNDLVAFVKAIIEKLK
HHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRVAILIENLYEEPELLYPYYRLKEEGYEVHLVGTEKDKVYTGKYGYPMKSTHASKEVK
CCHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCC
ADDYDAVVIPGGYSPDHMRRCQDTVNFVKEMCQQQKIIAAICHGPWMMASSCDLKGKRVT
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHH
SFFSIKDDLINAGAQYVDEEVVIDGNLITSRTPNDLVAFVKAIIEKLK
HHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]