Click here to switch to the map view.

The map label for this gene is AprE2 [H]

Identifier: 20807306

GI number: 20807306

Start: 831297

End: 832982

Strand: Reverse

Name: AprE2 [H]

Synonym: TTE0824

Alternate gene names: 20807306

Gene position: 832982-831297 (Counterclockwise)

Preceding gene: 20807308

Following gene: 20807305

Centisome position: 30.97

GC content: 37.49

Gene sequence:

>1686_bases
ATGAAAAAACATCAGTTAGCAAAAATTTTACTTTCTCTAGCGCTAATTATAAGTCTAATTTCGCTAAACGAAATACTAGT
ACAAGCTCAACCTAACCAAATAAACCTGCCTATAGATTCTCCTTCTAAAATTTACCCTTCTTTACCTCAAAAAATTTCAA
TGCAAGATTCCAATAAAAATAAAATTTTTGACGACTTAGAGCAAAGACTGCTTAATAAACCAGATAGCGAAGAATTCCCT
GTTATAATAACCTTTAACAAACCCGTAAGTGATGCAGATATATTCACTATCGCAAAAAACATCGGAAAATTTAATATAAA
ACATAGATATAAAATTATACCCTCAATTGCTGCCAATCTAACTAAAAGTCAAATTAATGTATTATCTAAATTAGAAATAG
TAAAGCAAATTGAGTACGATGAGCCTGTATATGCTACTTTAGATACCGCAACAAAGTGGTTTGGAATCACAAAAGCACGG
AGTGATTTTGGAGTCACAGGAAAAAATATAACAATAGCAATTATTGACACAGGTATAGACGGAAATCACGTTGACCTCTC
AGGTGGAAAAATAATAGGATGGAAAGACTTTATCAACAACAAAACTACACCATACGACGACAATGGCCATGGAACTCACG
TAGCAAGTATTGCTGCAGGTACAGGTGCTGGAAACAGTTTTTACAAAGGCGTTGCTCCTGATGCTTTGTTGGTAGGAATA
AAAGTTTTAGATGCAAATGGAAGCGGCAGCATGAGCACTGTAACTGCAGGAATTGACTGGGCTGTTCAAAATAAAGATGT
ATACGGAATCAAAGTTATAAATTTAAGCCTCGGCACTTCTACAAGTTCTGATGGAACTGACTCTACCTCATTAGCAGTGA
ATAGAGCAGTAGATAGCGGTATTGTAGTAGTTGTAGCAGCAGGAAACTCTGGCCCTGCAAAATACACCATAGGGTCCCCT
GGTGCTGCGGAAAAAGCCATAACAGTCGCAGCAATGGCAGATGTAGGTGAACTTGGCTTTAACCTTGCAAGCTTTTCCAG
CCGCGGTCCTACTGCTGACGGAAGAATAAAACCTGACATTGCGGCCCCAGGATATAATATAACTGCCGCAAAGGCGAATT
CTGTAAATGGATATGTAACATACAGCGGTACAAGCATGGCAACACCTTTTGTAGCAGGAACTGTTGCTCTTATGCTTAAC
GCTAATCCAAATCTCACTCCAAATGATGCAAAAAATATAATAATGTCTACTGCAAAAAGCTGGGGGCCTCCAAGCAAAAA
CGTTGACTATGGTGCAGGAAGATTGGATGGCTATGAAGCTATAAGGGTAGCAGGTAATTTTAGAGGAAATAATATTGATG
TACCAAATCATTATTATATTTCAGGTTATCTGCCCGGTTCTCGTTACAGTGATACTTGGACTTTTAACGCAACAAATACA
AGTTATCCAATCGCAATAACATTAATAATTCCTGACTGGGCAAATTACAATCCCGATTTTGATATATACCTCTATGATCC
AAGCGGCACACTTATCAAAAGCTCTACAGGGACTCAAAGGCAAGAAACAATAACAATACTTCCTTCACAAACTGGTACCT
ACTATGTTAAAGTTTATTCATATAGAGGTAGTGGCAATTATTTCTTTGATTTAAGCGCTGGAGGTAGTGAACCTGTAAAA
TATTAA

Upstream 100 bases:

>100_bases
TTTTATAAAAGATATTTTTGAAGGGGGTGATAATCATTAAGTGAAAAATTTTCTTCAATAGAACAAGAGTAAACTTATTT
TTAAGGAGGTATGAAGAAAA

Downstream 100 bases:

>100_bases
GTAGTAATACCTCTTAAAATAACCCAACCCTGTTGAGCAAGGAATCCCTTGCTCAACAGGGTTAATTAGGACTGAACCAT
CAAGATACCTTTACTAACTT

Product: subtilisin-like serine protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MKKHQLAKILLSLALIISLISLNEILVQAQPNQINLPIDSPSKIYPSLPQKISMQDSNKNKIFDDLEQRLLNKPDSEEFP
VIITFNKPVSDADIFTIAKNIGKFNIKHRYKIIPSIAANLTKSQINVLSKLEIVKQIEYDEPVYATLDTATKWFGITKAR
SDFGVTGKNITIAIIDTGIDGNHVDLSGGKIIGWKDFINNKTTPYDDNGHGTHVASIAAGTGAGNSFYKGVAPDALLVGI
KVLDANGSGSMSTVTAGIDWAVQNKDVYGIKVINLSLGTSTSSDGTDSTSLAVNRAVDSGIVVVVAAGNSGPAKYTIGSP
GAAEKAITVAAMADVGELGFNLASFSSRGPTADGRIKPDIAAPGYNITAAKANSVNGYVTYSGTSMATPFVAGTVALMLN
ANPNLTPNDAKNIIMSTAKSWGPPSKNVDYGAGRLDGYEAIRVAGNFRGNNIDVPNHYYISGYLPGSRYSDTWTFNATNT
SYPIAITLIIPDWANYNPDFDIYLYDPSGTLIKSSTGTQRQETITILPSQTGTYYVKVYSYRGSGNYFFDLSAGGSEPVK
Y

Sequences:

>Translated_561_residues
MKKHQLAKILLSLALIISLISLNEILVQAQPNQINLPIDSPSKIYPSLPQKISMQDSNKNKIFDDLEQRLLNKPDSEEFP
VIITFNKPVSDADIFTIAKNIGKFNIKHRYKIIPSIAANLTKSQINVLSKLEIVKQIEYDEPVYATLDTATKWFGITKAR
SDFGVTGKNITIAIIDTGIDGNHVDLSGGKIIGWKDFINNKTTPYDDNGHGTHVASIAAGTGAGNSFYKGVAPDALLVGI
KVLDANGSGSMSTVTAGIDWAVQNKDVYGIKVINLSLGTSTSSDGTDSTSLAVNRAVDSGIVVVVAAGNSGPAKYTIGSP
GAAEKAITVAAMADVGELGFNLASFSSRGPTADGRIKPDIAAPGYNITAAKANSVNGYVTYSGTSMATPFVAGTVALMLN
ANPNLTPNDAKNIIMSTAKSWGPPSKNVDYGAGRLDGYEAIRVAGNFRGNNIDVPNHYYISGYLPGSRYSDTWTFNATNT
SYPIAITLIIPDWANYNPDFDIYLYDPSGTLIKSSTGTQRQETITILPSQTGTYYVKVYSYRGSGNYFFDLSAGGSEPVK
Y
>Mature_561_residues
MKKHQLAKILLSLALIISLISLNEILVQAQPNQINLPIDSPSKIYPSLPQKISMQDSNKNKIFDDLEQRLLNKPDSEEFP
VIITFNKPVSDADIFTIAKNIGKFNIKHRYKIIPSIAANLTKSQINVLSKLEIVKQIEYDEPVYATLDTATKWFGITKAR
SDFGVTGKNITIAIIDTGIDGNHVDLSGGKIIGWKDFINNKTTPYDDNGHGTHVASIAAGTGAGNSFYKGVAPDALLVGI
KVLDANGSGSMSTVTAGIDWAVQNKDVYGIKVINLSLGTSTSSDGTDSTSLAVNRAVDSGIVVVVAAGNSGPAKYTIGSP
GAAEKAITVAAMADVGELGFNLASFSSRGPTADGRIKPDIAAPGYNITAAKANSVNGYVTYSGTSMATPFVAGTVALMLN
ANPNLTPNDAKNIIMSTAKSWGPPSKNVDYGAGRLDGYEAIRVAGNFRGNNIDVPNHYYISGYLPGSRYSDTWTFNATNT
SYPIAITLIIPDWANYNPDFDIYLYDPSGTLIKSSTGTQRQETITILPSQTGTYYVKVYSYRGSGNYFFDLSAGGSEPVK
Y

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI186972143, Length=318, Percent_Identity=30.188679245283, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI4506775, Length=297, Percent_Identity=28.956228956229, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI31317307, Length=357, Percent_Identity=26.890756302521, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI299523015, Length=282, Percent_Identity=26.241134751773, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI20336246, Length=282, Percent_Identity=26.241134751773, Blast_Score=70, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17533391, Length=228, Percent_Identity=28.9473684210526, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25141268, Length=503, Percent_Identity=24.2544731610338, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71983555, Length=380, Percent_Identity=23.4210526315789, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988301, Length=295, Percent_Identity=26.7796610169492, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988315, Length=295, Percent_Identity=26.4406779661017, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988308, Length=295, Percent_Identity=26.4406779661017, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988297, Length=295, Percent_Identity=26.4406779661017, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71988321, Length=295, Percent_Identity=26.7796610169492, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71988312, Length=295, Percent_Identity=26.4406779661017, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324576, Length=246, Percent_Identity=37.3983739837398, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320775, Length=250, Percent_Identity=37.6, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6319893, Length=238, Percent_Identity=36.1344537815126, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI45550681, Length=298, Percent_Identity=27.5167785234899, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17137360, Length=234, Percent_Identity=32.9059829059829, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24653270, Length=234, Percent_Identity=32.9059829059829, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17136192, Length=276, Percent_Identity=28.2608695652174, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864552, Length=326, Percent_Identity=24.8466257668712, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24650056, Length=327, Percent_Identity=24.7706422018349, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45553515, Length=326, Percent_Identity=24.8466257668712, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24650058, Length=326, Percent_Identity=24.8466257668712, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24650060, Length=326, Percent_Identity=24.8466257668712, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45553513, Length=326, Percent_Identity=24.8466257668712, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 59969; Mature: 59969

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00018 EF_HAND_1 ; PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHQLAKILLSLALIISLISLNEILVQAQPNQINLPIDSPSKIYPSLPQKISMQDSNKN
CCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEECCCCCCCCCCCCCCCEEECCCCCCC
KIFDDLEQRLLNKPDSEEFPVIITFNKPVSDADIFTIAKNIGKFNIKHRYKIIPSIAANL
CHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEEEECCHHHHHH
TKSQINVLSKLEIVKQIEYDEPVYATLDTATKWFGITKARSDFGVTGKNITIAIIDTGID
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHEEEEECCCCCCCCCCCEEEEEEECCCC
GNHVDLSGGKIIGWKDFINNKTTPYDDNGHGTHVASIAAGTGAGNSFYKGVAPDALLVGI
CCEEECCCCEEEEEHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEE
KVLDANGSGSMSTVTAGIDWAVQNKDVYGIKVINLSLGTSTSSDGTDSTSLAVNRAVDSG
EEEECCCCCCCEEEEECEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCC
IVVVVAAGNSGPAKYTIGSPGAAEKAITVAAMADVGELGFNLASFSSRGPTADGRIKPDI
EEEEEECCCCCCEEEEECCCCCCCCEEEEEEEHHHHHHCCEEECCCCCCCCCCCEECCCC
AAPGYNITAAKANSVNGYVTYSGTSMATPFVAGTVALMLNANPNLTPNDAKNIIMSTAKS
CCCCCEEEEEECCCCCEEEEECCCCCCCCHHEEEEEEEEECCCCCCCCHHHHHHHHHHHH
WGPPSKNVDYGAGRLDGYEAIRVAGNFRGNNIDVPNHYYISGYLPGSRYSDTWTFNATNT
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCC
SYPIAITLIIPDWANYNPDFDIYLYDPSGTLIKSSTGTQRQETITILPSQTGTYYVKVYS
CCCEEEEEEECCCCCCCCCEEEEEECCCCCEEECCCCCCCCEEEEEEECCCCEEEEEEEE
YRGSGNYFFDLSAGGSEPVKY
EECCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MKKHQLAKILLSLALIISLISLNEILVQAQPNQINLPIDSPSKIYPSLPQKISMQDSNKN
CCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEECCCCCCCCCCCCCCCEEECCCCCCC
KIFDDLEQRLLNKPDSEEFPVIITFNKPVSDADIFTIAKNIGKFNIKHRYKIIPSIAANL
CHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEEEECCHHHHHH
TKSQINVLSKLEIVKQIEYDEPVYATLDTATKWFGITKARSDFGVTGKNITIAIIDTGID
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHEEEEECCCCCCCCCCCEEEEEEECCCC
GNHVDLSGGKIIGWKDFINNKTTPYDDNGHGTHVASIAAGTGAGNSFYKGVAPDALLVGI
CCEEECCCCEEEEEHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEE
KVLDANGSGSMSTVTAGIDWAVQNKDVYGIKVINLSLGTSTSSDGTDSTSLAVNRAVDSG
EEEECCCCCCCEEEEECEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCC
IVVVVAAGNSGPAKYTIGSPGAAEKAITVAAMADVGELGFNLASFSSRGPTADGRIKPDI
EEEEEECCCCCCEEEEECCCCCCCCEEEEEEEHHHHHHCCEEECCCCCCCCCCCEECCCC
AAPGYNITAAKANSVNGYVTYSGTSMATPFVAGTVALMLNANPNLTPNDAKNIIMSTAKS
CCCCCEEEEEECCCCCEEEEECCCCCCCCHHEEEEEEEEECCCCCCCCHHHHHHHHHHHH
WGPPSKNVDYGAGRLDGYEAIRVAGNFRGNNIDVPNHYYISGYLPGSRYSDTWTFNATNT
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCC
SYPIAITLIIPDWANYNPDFDIYLYDPSGTLIKSSTGTQRQETITILPSQTGTYYVKVYS
CCCEEEEEEECCCCCCCCCEEEEEECCCCCEEECCCCCCCCEEEEEEECCCCEEEEEEEE
YRGSGNYFFDLSAGGSEPVKY
EECCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]