Click here to switch to the map view.

The map label for this gene is PurF [H]

Identifier: 20807294

GI number: 20807294

Start: 811056

End: 812453

Strand: Direct

Name: PurF [H]

Synonym: TTE0812

Alternate gene names: 20807294

Gene position: 811056-812453 (Clockwise)

Preceding gene: 20807293

Following gene: 20807295

Centisome position: 30.16

GC content: 35.55

Gene sequence:

>1398_bases
ATGGGAAATGGAGAGAAATTAAAAGAGGAATGTGGTGTTTTTGGTGCCTTTAGTTTGTCCACTTCAGTTACTTCTTATAT
CTATTATGGCTTACAAGCCTTGCAGCACAGAGGGCAGGAAAGTTCAGGTATAGCTATTTATGATGGCGAAAAAATAAATT
GTATTAAGGGGTTGGGACTTGTCAGCGAGGTCTTTAATAAGGAGAATTTAAAGACATTAGAGGGGAAAATGGGGATAGGC
CATGTAAGGTATTCTACTACAGGCAGCAATAACATTATAAATGCTCAACCCCTCGTTGCTAATTTTAAAAACAAATATAT
GGCTCTTGCCCATAATGGCAATTTGATAAACGCAGAAGAGTTAAGATGCCTATTGGAGGAGGATGGTAGAATATTTCAAA
CTACAACTGATAGTGAAATAATTCTCCATCTAATTGCAAAAAATTTTCAAGAAAACTTAATCGGAGCTCTTTTAGAAACA
ATGAAACAAATAAAAGGTTCATATGCTTTGGTGATTTTAACAGACAATAAATTAATAGGGATAAGAGACGTAAACAGTAT
AAGGCCTCTTTGCATAGGTAAAAAAGATGACACTTATTTCTTATCTTCTGAGTCTTGTGCTTTTGACGTAATAGGAGCAG
AATTGATACGAGATGTGGAAGCAGGTGAAATAGTTATAATTGATGAAAAAGGTATAGATTCTGTTAAGTTAGAAGTAAAA
GAAAAAAAGAAGCCTTGTGTTTTTGAATACATCTATTTTGCTAGACCTGACAGTGTCATAGATGGAAAAAGTGTTTATTT
TACACGATTGGAGATGGGGAAAAGATTAGCTGAAGAAGCACCTGTAGATGCAGACTTGGTAGTACCAGTTCCAGATTCAG
GTATTGCTGCTGCAAGAGGCTATTCTCTCAAAACAGGGATACCAATGGGGGAAGGACTTATAAAAAATAAATACATTGGA
AGGACTTTTATTGCTCCCGACCAAAGGGATAGAGAGACAGGCGTGAGGATAAAGCTAAATGTCTTAAAAGAATTGGTTCA
AGGCAAAAGGATAGTTTTAATAGATGATTCAATTGTTAGAGGCACAACTATGAAAAGATTAGTGAGTCTACTAAAAAATG
GCGGAGCAAAAGAAGTCCATGTGAGGATAAGTTCACCCCCTGTTAAATATTCTTGCTACTTTGGAATTGATACTCCGACA
AAAAAAGAACTGATAGCGGCGAGGATGTCAGTTGAAGAGATGTGCAAATTAATAGGAGCTGACAGCCTACAGTTTTTAAG
TATTGAAGGGCTTATAAAAAGTGTAGGACTTAAATCTATTTGCACAGGTTGCTTTGATGGCAATTATCCTATGTATGTTC
CAAAAGAAAGAAGTAAATATCTTTTTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
GAGTTGTAGAGGGTAAAGATATTGTAATTGATGTAAATGGCAAAAGAATAATAGACCTTCCTTTAGAAGTCTTAGAGGAA
AGCTGGAGAGGGAGAATAAA

Downstream 100 bases:

>100_bases
AAAGGGTGAGAGAAAGTTAATTAAACCTAAAAGCTTATACTCACAAGAATTGATGAAAAAGGAGAAAATCACGATATGCC
GCGTATTGAAAAAGATTTCT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGNGEKLKEECGVFGAFSLSTSVTSYIYYGLQALQHRGQESSGIAIYDGEKINCIKGLGLVSEVFNKENLKTLEGKMGIG
HVRYSTTGSNNIINAQPLVANFKNKYMALAHNGNLINAEELRCLLEEDGRIFQTTTDSEIILHLIAKNFQENLIGALLET
MKQIKGSYALVILTDNKLIGIRDVNSIRPLCIGKKDDTYFLSSESCAFDVIGAELIRDVEAGEIVIIDEKGIDSVKLEVK
EKKKPCVFEYIYFARPDSVIDGKSVYFTRLEMGKRLAEEAPVDADLVVPVPDSGIAAARGYSLKTGIPMGEGLIKNKYIG
RTFIAPDQRDRETGVRIKLNVLKELVQGKRIVLIDDSIVRGTTMKRLVSLLKNGGAKEVHVRISSPPVKYSCYFGIDTPT
KKELIAARMSVEEMCKLIGADSLQFLSIEGLIKSVGLKSICTGCFDGNYPMYVPKERSKYLFEKK

Sequences:

>Translated_465_residues
MGNGEKLKEECGVFGAFSLSTSVTSYIYYGLQALQHRGQESSGIAIYDGEKINCIKGLGLVSEVFNKENLKTLEGKMGIG
HVRYSTTGSNNIINAQPLVANFKNKYMALAHNGNLINAEELRCLLEEDGRIFQTTTDSEIILHLIAKNFQENLIGALLET
MKQIKGSYALVILTDNKLIGIRDVNSIRPLCIGKKDDTYFLSSESCAFDVIGAELIRDVEAGEIVIIDEKGIDSVKLEVK
EKKKPCVFEYIYFARPDSVIDGKSVYFTRLEMGKRLAEEAPVDADLVVPVPDSGIAAARGYSLKTGIPMGEGLIKNKYIG
RTFIAPDQRDRETGVRIKLNVLKELVQGKRIVLIDDSIVRGTTMKRLVSLLKNGGAKEVHVRISSPPVKYSCYFGIDTPT
KKELIAARMSVEEMCKLIGADSLQFLSIEGLIKSVGLKSICTGCFDGNYPMYVPKERSKYLFEKK
>Mature_464_residues
GNGEKLKEECGVFGAFSLSTSVTSYIYYGLQALQHRGQESSGIAIYDGEKINCIKGLGLVSEVFNKENLKTLEGKMGIGH
VRYSTTGSNNIINAQPLVANFKNKYMALAHNGNLINAEELRCLLEEDGRIFQTTTDSEIILHLIAKNFQENLIGALLETM
KQIKGSYALVILTDNKLIGIRDVNSIRPLCIGKKDDTYFLSSESCAFDVIGAELIRDVEAGEIVIIDEKGIDSVKLEVKE
KKKPCVFEYIYFARPDSVIDGKSVYFTRLEMGKRLAEEAPVDADLVVPVPDSGIAAARGYSLKTGIPMGEGLIKNKYIGR
TFIAPDQRDRETGVRIKLNVLKELVQGKRIVLIDDSIVRGTTMKRLVSLLKNGGAKEVHVRISSPPVKYSCYFGIDTPTK
KELIAARMSVEEMCKLIGADSLQFLSIEGLIKSVGLKSICTGCFDGNYPMYVPKERSKYLFEKK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=506, Percent_Identity=40.7114624505929, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI4826742, Length=187, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI205277386, Length=189, Percent_Identity=27.5132275132275, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788651, Length=451, Percent_Identity=40.7982261640798, Blast_Score=321, Evalue=8e-89,
Organism=Escherichia coli, GI1790167, Length=246, Percent_Identity=29.2682926829268, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=41.0041841004184, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI17532897, Length=216, Percent_Identity=29.6296296296296, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17532899, Length=216, Percent_Identity=29.6296296296296, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539970, Length=223, Percent_Identity=29.5964125560538, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323958, Length=471, Percent_Identity=39.2781316348195, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6322745, Length=212, Percent_Identity=31.1320754716981, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323730, Length=209, Percent_Identity=27.7511961722488, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI28573187, Length=496, Percent_Identity=42.741935483871, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24659598, Length=490, Percent_Identity=41.6326530612245, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24659604, Length=468, Percent_Identity=42.3076923076923, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI21357745, Length=182, Percent_Identity=28.021978021978, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51430; Mature: 51299

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNGEKLKEECGVFGAFSLSTSVTSYIYYGLQALQHRGQESSGIAIYDGEKINCIKGLGL
CCCHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEHHHHHHH
VSEVFNKENLKTLEGKMGIGHVRYSTTGSNNIINAQPLVANFKNKYMALAHNGNLINAEE
HHHHHCCCCCEECCCCCCCCEEEEECCCCCCEEECCCHHHCCCCCEEEEEECCCEECHHH
LRCLLEEDGRIFQTTTDSEIILHLIAKNFQENLIGALLETMKQIKGSYALVILTDNKLIG
HHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEE
IRDVNSIRPLCIGKKDDTYFLSSESCAFDVIGAELIRDVEAGEIVIIDEKGIDSVKLEVK
EECCCCCCCEEECCCCCEEEECCCCCEEEHHHHHHHHCCCCCEEEEEECCCCCEEEEEEE
EKKKPCVFEYIYFARPDSVIDGKSVYFTRLEMGKRLAEEAPVDADLVVPVPDSGIAAARG
CCCCCEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCC
YSLKTGIPMGEGLIKNKYIGRTFIAPDQRDRETGVRIKLNVLKELVQGKRIVLIDDSIVR
CEEECCCCCCCCHHCCCCCCCEECCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCHHC
GTTMKRLVSLLKNGGAKEVHVRISSPPVKYSCYFGIDTPTKKELIAARMSVEEMCKLIGA
CHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHCC
DSLQFLSIEGLIKSVGLKSICTGCFDGNYPMYVPKERSKYLFEKK
CCEEEEEHHHHHHHCCHHHHHHHHCCCCCCEEECCCHHCCCCCCC
>Mature Secondary Structure 
GNGEKLKEECGVFGAFSLSTSVTSYIYYGLQALQHRGQESSGIAIYDGEKINCIKGLGL
CCHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEHHHHHHH
VSEVFNKENLKTLEGKMGIGHVRYSTTGSNNIINAQPLVANFKNKYMALAHNGNLINAEE
HHHHHCCCCCEECCCCCCCCEEEEECCCCCCEEECCCHHHCCCCCEEEEEECCCEECHHH
LRCLLEEDGRIFQTTTDSEIILHLIAKNFQENLIGALLETMKQIKGSYALVILTDNKLIG
HHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEE
IRDVNSIRPLCIGKKDDTYFLSSESCAFDVIGAELIRDVEAGEIVIIDEKGIDSVKLEVK
EECCCCCCCEEECCCCCEEEECCCCCEEEHHHHHHHHCCCCCEEEEEECCCCCEEEEEEE
EKKKPCVFEYIYFARPDSVIDGKSVYFTRLEMGKRLAEEAPVDADLVVPVPDSGIAAARG
CCCCCEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCC
YSLKTGIPMGEGLIKNKYIGRTFIAPDQRDRETGVRIKLNVLKELVQGKRIVLIDDSIVR
CEEECCCCCCCCHHCCCCCCCEECCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCHHC
GTTMKRLVSLLKNGGAKEVHVRISSPPVKYSCYFGIDTPTKKELIAARMSVEEMCKLIGA
CHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHCC
DSLQFLSIEGLIKSVGLKSICTGCFDGNYPMYVPKERSKYLFEKK
CCEEEEEHHHHHHHCCHHHHHHHHCCCCCCEEECCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]