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The map label for this gene is aspA [H]

Identifier: 20807295

GI number: 20807295

Start: 812529

End: 813914

Strand: Direct

Name: aspA [H]

Synonym: TTE0813

Alternate gene names: 20807295

Gene position: 812529-813914 (Clockwise)

Preceding gene: 20807294

Following gene: 20807296

Centisome position: 30.21

GC content: 37.95

Gene sequence:

>1386_bases
ATGCCGCGTATTGAAAAAGATTTCTTGGGTGAATTAACACTTGAAGATAATGAACTTTATGGAATTCATACAAAACGAGC
TTTGAAAAACTTCTCTGTATCTTCACAAAAGATTGATATAGATCTTGTACGAGCAATTGTGATGGTCAAAAAAGCATGCG
CAATTGCAAATTTTGAGGTTGGAAACCTCGATGAAAAAATAAAAGATGCAATTGTCTTTGCATGTGATGAGATTTTAGCC
GGCAAATACACCGAGCAATTTGTGGTAGACAAATTCCAAGGCGGTGCTGGAACATCTACAAATATGAACGTAAATGAAGT
AATTGCCAACATAGCACTCATTTATCTTGGCAAAAAACCAGGAGAGTATCAATACATCCATCCTATAGACCATGTTAATA
TGTCACAATCGACAAATGATGTCTACCCAACCGCTTTGAGGATTGCAACAATATGGAATGTGAGAGAACTTTCTGAAGAG
TGTGCAGAACTCCAAAAAAGCTTGCAAAAAAAAGAGCATGAGTTTGAAGGTGTAATTAAGGCTGGGCGCACTCAACTTCA
AGATGCCCTTCCCATCACCTTAGGGCAAGAGTTTGGGGCGTATGCACAGGCGATATCACGCGATAGATGGCGAATATATA
AAATTGAAGAAAGATTAAGAGTGGTAAACTTGGGCGCAACAGCTGTTGGAACAGGAGTGAATGCAAGCTTAAAATATATG
TTTAAAGTGATAGAACTTTTGCGCGATTTTACTAAAATAGGCTTGGCTCGGTCTGATTACCTCATGGATGCAACACAAAA
TGCAGATGTATTTGTGGAATGCTCGGGTCTTTTAAAAGCCTTGGCGGTGAATCTATCAAAAATTGCAAATGACTTAAGGC
TCTTATCATCTGGTCCAAACACGGGTTTAAACGAGATAAACTTACCTGCTGCACAAGCAGGTTCAAGCATTATGCCTGGT
AAAGTAAATCCTGTCATACCTGAACTTATAAACACCATATCATTCCAGGTTATGTCAAATGATGTTGCAATAACCTTAGC
AGCCCAAGCAGGTCAGCTTGAGCTAAATGCATTCTTGCCTTTAATTGCAAACAATTTGCTTGAAAGCCTCAAAATTCTCA
AAAACGGAATCAGGATATTCAGAAAACAGTGCATTGATGGAATAACAGCAAACAAAGAAAGATGTCTTGAGTTTGCCAAA
AAGACTCCTTCTATTGCCGCTGCATTGATTGATAAAATAGGGTATGACAAGGCAAGTGAAATTGCTAAAAAAGCCATTGC
CGAGAATAAGGAAATAATTGAGGTGGTAAAGGAATTGAAGATCTTAAATGAGAATGAGGCTGAAGCACTACTAAATCCTT
TTGAATTTGTAAAGTTTAAAGAGTAA

Upstream 100 bases:

>100_bases
TAAATATCTTTTTGAGAAAAAATAAAAAGGGTGAGAGAAAGTTAATTAAACCTAAAAGCTTATACTCACAAGAATTGATG
AAAAAGGAGAAAATCACGAT

Downstream 100 bases:

>100_bases
AAAATTTTGAAAGGGCTGAAAAAGATGAACACAACACCGCGAAGCGAAAGGCTTCATATAGCAATATTTGGAAAAAGAAA
TGCTGGAAAATCAAGTTTGA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MPRIEKDFLGELTLEDNELYGIHTKRALKNFSVSSQKIDIDLVRAIVMVKKACAIANFEVGNLDEKIKDAIVFACDEILA
GKYTEQFVVDKFQGGAGTSTNMNVNEVIANIALIYLGKKPGEYQYIHPIDHVNMSQSTNDVYPTALRIATIWNVRELSEE
CAELQKSLQKKEHEFEGVIKAGRTQLQDALPITLGQEFGAYAQAISRDRWRIYKIEERLRVVNLGATAVGTGVNASLKYM
FKVIELLRDFTKIGLARSDYLMDATQNADVFVECSGLLKALAVNLSKIANDLRLLSSGPNTGLNEINLPAAQAGSSIMPG
KVNPVIPELINTISFQVMSNDVAITLAAQAGQLELNAFLPLIANNLLESLKILKNGIRIFRKQCIDGITANKERCLEFAK
KTPSIAAALIDKIGYDKASEIAKKAIAENKEIIEVVKELKILNENEAEALLNPFEFVKFKE

Sequences:

>Translated_461_residues
MPRIEKDFLGELTLEDNELYGIHTKRALKNFSVSSQKIDIDLVRAIVMVKKACAIANFEVGNLDEKIKDAIVFACDEILA
GKYTEQFVVDKFQGGAGTSTNMNVNEVIANIALIYLGKKPGEYQYIHPIDHVNMSQSTNDVYPTALRIATIWNVRELSEE
CAELQKSLQKKEHEFEGVIKAGRTQLQDALPITLGQEFGAYAQAISRDRWRIYKIEERLRVVNLGATAVGTGVNASLKYM
FKVIELLRDFTKIGLARSDYLMDATQNADVFVECSGLLKALAVNLSKIANDLRLLSSGPNTGLNEINLPAAQAGSSIMPG
KVNPVIPELINTISFQVMSNDVAITLAAQAGQLELNAFLPLIANNLLESLKILKNGIRIFRKQCIDGITANKERCLEFAK
KTPSIAAALIDKIGYDKASEIAKKAIAENKEIIEVVKELKILNENEAEALLNPFEFVKFKE
>Mature_460_residues
PRIEKDFLGELTLEDNELYGIHTKRALKNFSVSSQKIDIDLVRAIVMVKKACAIANFEVGNLDEKIKDAIVFACDEILAG
KYTEQFVVDKFQGGAGTSTNMNVNEVIANIALIYLGKKPGEYQYIHPIDHVNMSQSTNDVYPTALRIATIWNVRELSEEC
AELQKSLQKKEHEFEGVIKAGRTQLQDALPITLGQEFGAYAQAISRDRWRIYKIEERLRVVNLGATAVGTGVNASLKYMF
KVIELLRDFTKIGLARSDYLMDATQNADVFVECSGLLKALAVNLSKIANDLRLLSSGPNTGLNEINLPAAQAGSSIMPGK
VNPVIPELINTISFQVMSNDVAITLAAQAGQLELNAFLPLIANNLLESLKILKNGIRIFRKQCIDGITANKERCLEFAKK
TPSIAAALIDKIGYDKASEIAKKAIAENKEIIEVVKELKILNENEAEALLNPFEFVKFKE

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=39.4793926247288, Blast_Score=317, Evalue=2e-86,
Organism=Escherichia coli, GI87082375, Length=447, Percent_Identity=47.427293064877, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1787896, Length=456, Percent_Identity=39.2543859649123, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1790398, Length=305, Percent_Identity=26.5573770491803, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17553882, Length=454, Percent_Identity=40.3083700440529, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI32565146, Length=321, Percent_Identity=39.2523364485981, Blast_Score=231, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6324993, Length=454, Percent_Identity=40.7488986784141, Blast_Score=338, Evalue=1e-93,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=40.6047516198704, Blast_Score=337, Evalue=8e-93,
Organism=Drosophila melanogaster, GI24640177, Length=462, Percent_Identity=40.04329004329, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24640179, Length=462, Percent_Identity=40.04329004329, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24662684, Length=428, Percent_Identity=41.1214953271028, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24583245, Length=428, Percent_Identity=37.1495327102804, Blast_Score=295, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51010; Mature: 50878

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIEKDFLGELTLEDNELYGIHTKRALKNFSVSSQKIDIDLVRAIVMVKKACAIANFEV
CCCCCCHHCCCEEECCCCEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GNLDEKIKDAIVFACDEILAGKYTEQFVVDKFQGGAGTSTNMNVNEVIANIALIYLGKKP
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEECCCC
GEYQYIHPIDHVNMSQSTNDVYPTALRIATIWNVRELSEECAELQKSLQKKEHEFEGVIK
CCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRTQLQDALPITLGQEFGAYAQAISRDRWRIYKIEERLRVVNLGATAVGTGVNASLKYM
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHEEEECCCHHHCCCCCHHHHHH
FKVIELLRDFTKIGLARSDYLMDATQNADVFVECSGLLKALAVNLSKIANDLRLLSSGPN
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
TGLNEINLPAAQAGSSIMPGKVNPVIPELINTISFQVMSNDVAITLAAQAGQLELNAFLP
CCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHEEECCCEEEEEECCCCCEEHHHHHH
LIANNLLESLKILKNGIRIFRKQCIDGITANKERCLEFAKKTPSIAAALIDKIGYDKASE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHH
IAKKAIAENKEIIEVVKELKILNENEAEALLNPFEFVKFKE
HHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHCHHHHHEECC
>Mature Secondary Structure 
PRIEKDFLGELTLEDNELYGIHTKRALKNFSVSSQKIDIDLVRAIVMVKKACAIANFEV
CCCCCHHCCCEEECCCCEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GNLDEKIKDAIVFACDEILAGKYTEQFVVDKFQGGAGTSTNMNVNEVIANIALIYLGKKP
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEECCCC
GEYQYIHPIDHVNMSQSTNDVYPTALRIATIWNVRELSEECAELQKSLQKKEHEFEGVIK
CCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRTQLQDALPITLGQEFGAYAQAISRDRWRIYKIEERLRVVNLGATAVGTGVNASLKYM
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHEEEECCCHHHCCCCCHHHHHH
FKVIELLRDFTKIGLARSDYLMDATQNADVFVECSGLLKALAVNLSKIANDLRLLSSGPN
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
TGLNEINLPAAQAGSSIMPGKVNPVIPELINTISFQVMSNDVAITLAAQAGQLELNAFLP
CCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHEEECCCEEEEEECCCCCEEHHHHHH
LIANNLLESLKILKNGIRIFRKQCIDGITANKERCLEFAKKTPSIAAALIDKIGYDKASE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHH
IAKKAIAENKEIIEVVKELKILNENEAEALLNPFEFVKFKE
HHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]