Click here to switch to the map view.

The map label for this gene is AceF2 [H]

Identifier: 20807177

GI number: 20807177

Start: 694595

End: 695794

Strand: Direct

Name: AceF2 [H]

Synonym: TTE0690

Alternate gene names: 20807177

Gene position: 694595-695794 (Clockwise)

Preceding gene: 20807176

Following gene: 20807178

Centisome position: 25.83

GC content: 45.67

Gene sequence:

>1200_bases
ATGCCATACGAATTTCGCATGCCTAACGTAGTATCAAATGGAATAGAAGGGTTTGTGGTGAATTGGTTCAAGGATGAAGG
ACAGCCAGTACAAGCAGGTGAATTGCTGTTGGAAGTACAATTTGAAAAAGCGGCTATTGAACTCCAGGCTCCAGTAAGCG
GCATACTGACAAAAATCCTATGTCCCCAAGGTCATGTAGTCAAGGTTGGACAACTGCTCTGTTTGATCGAAGAAAAATCC
ACAGAAGTGGCTGGAGGTAGTGGATCTGCGGTACCTCCGGTCCATGCACCAGAGGAAACACCCCACTTCCACGGCGAAAC
GGAGCGAACAACCCAGAGTACTCCAGTGGATTCCCAAGTCCATAGTAGCAACACGGGCGATGTAAGAGCCACTCCTGCAG
CCCGTAAGCTCGCCCGCGAATTGGGAATTCCTCTAGAGGCAGTGCCGGGTACTGGTCCGGGCGGTCGAATTACTGAGGAA
GATGTTAAGAATTTTGCTCAGCGTTCTGAAAAGACAAATTTAACAGCAAGAAGAGTACCACTCACTCCTACCCAGCGTTT
AGTCGGGTCAAGGATGTTACAGAGTCTTCGAGAAACTGCACAGTATACATTAGGGCGGGAAGTAGATATAAGCGCGTTAA
TCAAAGTCCGCATGGAGCTTAAACAAAAAGGTTCACCTGCTAACATAACGGATCTTATACATAAAGCAGTGGTTCAGGCG
ATTTTAGAAAATCCTGTAATGCAGGCCGTTATTGATGGAGATGATATGGTAGTGCCTGCAGAAGTTCACTTGGGATTTGC
TGTTGCCCGTGGCGATGAACTTCTAGTGCCTGTAATCAAAAATGCTCATAGACTCAATCTTAATGAAATGGCTGTTGAAA
GACGCCGACTTACTGATGCAGTTCTACAAGGTATTATAAAGCCAGAAGAGCTACAAGGGGGTACCTTTACAGTTACAAAT
CTGGGTACTTATGGTGTTGATTTCTTTACCCCTGTGCTATATCCAAAACAATCTGCAATTCTCGGTATTGGAAGAATAGT
GGAACGACCAGTCCTTGAGAATGGAAACATCCGCTCCGCGCAATTTATGACTCTTAGTCTTACTGTAGATCATCAGGTAA
TAAACGGTGCCCCTGCTGCTAGATTCTTAAATCGACTGGCAGAACTTCTTTCTCAACCGGAAGTGCTCTTGGAGTTGTAA

Upstream 100 bases:

>100_bases
GAACAGTTTGTATTACCTTCAAGTTCTAAAATTGTGAATGCAGTTAAGGAATTGCTCAGGTCATAATAATGAGATTTCAA
AGCAGGAGGACTGATATCTT

Downstream 100 bases:

>100_bases
TAGCAAAAGGGCAGTGCATGTGCGCTGCCCTTTTAGAGTACATAAAAAAAGACAAGTTGTAGAAGAAGGTGAGCATGTAA
AGGTAATATTATCCAGTGGA

Product: dihydrolipoamide acyltransferase

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MPYEFRMPNVVSNGIEGFVVNWFKDEGQPVQAGELLLEVQFEKAAIELQAPVSGILTKILCPQGHVVKVGQLLCLIEEKS
TEVAGGSGSAVPPVHAPEETPHFHGETERTTQSTPVDSQVHSSNTGDVRATPAARKLARELGIPLEAVPGTGPGGRITEE
DVKNFAQRSEKTNLTARRVPLTPTQRLVGSRMLQSLRETAQYTLGREVDISALIKVRMELKQKGSPANITDLIHKAVVQA
ILENPVMQAVIDGDDMVVPAEVHLGFAVARGDELLVPVIKNAHRLNLNEMAVERRRLTDAVLQGIIKPEELQGGTFTVTN
LGTYGVDFFTPVLYPKQSAILGIGRIVERPVLENGNIRSAQFMTLSLTVDHQVINGAPAARFLNRLAELLSQPEVLLEL

Sequences:

>Translated_399_residues
MPYEFRMPNVVSNGIEGFVVNWFKDEGQPVQAGELLLEVQFEKAAIELQAPVSGILTKILCPQGHVVKVGQLLCLIEEKS
TEVAGGSGSAVPPVHAPEETPHFHGETERTTQSTPVDSQVHSSNTGDVRATPAARKLARELGIPLEAVPGTGPGGRITEE
DVKNFAQRSEKTNLTARRVPLTPTQRLVGSRMLQSLRETAQYTLGREVDISALIKVRMELKQKGSPANITDLIHKAVVQA
ILENPVMQAVIDGDDMVVPAEVHLGFAVARGDELLVPVIKNAHRLNLNEMAVERRRLTDAVLQGIIKPEELQGGTFTVTN
LGTYGVDFFTPVLYPKQSAILGIGRIVERPVLENGNIRSAQFMTLSLTVDHQVINGAPAARFLNRLAELLSQPEVLLEL
>Mature_398_residues
PYEFRMPNVVSNGIEGFVVNWFKDEGQPVQAGELLLEVQFEKAAIELQAPVSGILTKILCPQGHVVKVGQLLCLIEEKST
EVAGGSGSAVPPVHAPEETPHFHGETERTTQSTPVDSQVHSSNTGDVRATPAARKLARELGIPLEAVPGTGPGGRITEED
VKNFAQRSEKTNLTARRVPLTPTQRLVGSRMLQSLRETAQYTLGREVDISALIKVRMELKQKGSPANITDLIHKAVVQAI
LENPVMQAVIDGDDMVVPAEVHLGFAVARGDELLVPVIKNAHRLNLNEMAVERRRLTDAVLQGIIKPEELQGGTFTVTNL
GTYGVDFFTPVLYPKQSAILGIGRIVERPVLENGNIRSAQFMTLSLTVDHQVINGAPAARFLNRLAELLSQPEVLLEL

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=422, Percent_Identity=28.9099526066351, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=27.3333333333333, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=27.3333333333333, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=26.7281105990783, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI19923748, Length=261, Percent_Identity=30.6513409961686, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI260898739, Length=170, Percent_Identity=31.7647058823529, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786946, Length=419, Percent_Identity=30.7875894988067, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1786305, Length=399, Percent_Identity=25.31328320802, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560088, Length=415, Percent_Identity=31.3253012048193, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=28.175519630485, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=401, Percent_Identity=27.6807980049875, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=220, Percent_Identity=28.6363636363636, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=28.2850779510022, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320352, Length=400, Percent_Identity=29.25, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=31.858407079646, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24582497, Length=292, Percent_Identity=27.7397260273973, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859875, Length=412, Percent_Identity=25.4854368932039, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20129315, Length=292, Percent_Identity=27.7397260273973, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43415; Mature: 43283

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYEFRMPNVVSNGIEGFVVNWFKDEGQPVQAGELLLEVQFEKAAIELQAPVSGILTKIL
CCCCCCCCHHHHCCCCEEEEEEECCCCCCCCCCEEEEEEEECHHEEEEECCHHHHHHHHH
CPQGHVVKVGQLLCLIEEKSTEVAGGSGSAVPPVHAPEETPHFHGETERTTQSTPVDSQV
CCCCCEEECCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
HSSNTGDVRATPAARKLARELGIPLEAVPGTGPGGRITEEDVKNFAQRSEKTNLTARRVP
CCCCCCCEEECHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECC
LTPTQRLVGSRMLQSLRETAQYTLGREVDISALIKVRMELKQKGSPANITDLIHKAVVQA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ILENPVMQAVIDGDDMVVPAEVHLGFAVARGDELLVPVIKNAHRLNLNEMAVERRRLTDA
HHHCCHHEEEECCCCEEEEEHHEEEEEEECCCCEEEHHHCCCHHCCHHHHHHHHHHHHHH
VLQGIIKPEELQGGTFTVTNLGTYGVDFFTPVLYPKQSAILGIGRIVERPVLENGNIRSA
HHHHCCCHHHCCCCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEE
QFMTLSLTVDHQVINGAPAARFLNRLAELLSQPEVLLEL
EEEEEEEEECCHHHCCCHHHHHHHHHHHHHCCCCEEHCC
>Mature Secondary Structure 
PYEFRMPNVVSNGIEGFVVNWFKDEGQPVQAGELLLEVQFEKAAIELQAPVSGILTKIL
CCCCCCCHHHHCCCCEEEEEEECCCCCCCCCCEEEEEEEECHHEEEEECCHHHHHHHHH
CPQGHVVKVGQLLCLIEEKSTEVAGGSGSAVPPVHAPEETPHFHGETERTTQSTPVDSQV
CCCCCEEECCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
HSSNTGDVRATPAARKLARELGIPLEAVPGTGPGGRITEEDVKNFAQRSEKTNLTARRVP
CCCCCCCEEECHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECC
LTPTQRLVGSRMLQSLRETAQYTLGREVDISALIKVRMELKQKGSPANITDLIHKAVVQA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ILENPVMQAVIDGDDMVVPAEVHLGFAVARGDELLVPVIKNAHRLNLNEMAVERRRLTDA
HHHCCHHEEEECCCCEEEEEHHEEEEEEECCCCEEEHHHCCCHHCCHHHHHHHHHHHHHH
VLQGIIKPEELQGGTFTVTNLGTYGVDFFTPVLYPKQSAILGIGRIVERPVLENGNIRSA
HHHHCCCHHHCCCCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEE
QFMTLSLTVDHQVINGAPAARFLNRLAELLSQPEVLLEL
EEEEEEEEECCHHHCCCHHHHHHHHHHHHHCCCCEEHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]