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The map label for this gene is AlkA

Identifier: 20807070

GI number: 20807070

Start: 575165

End: 576058

Strand: Direct

Name: AlkA

Synonym: TTE0574

Alternate gene names: NA

Gene position: 575165-576058 (Clockwise)

Preceding gene: 20807069

Following gene: 20807071

Centisome position: 21.39

GC content: 38.37

Gene sequence:

>894_bases
ATGAAATATAATGTGGAAGAAAAGGGGACAAAGGTAATTGTAAGGGGGATTGCAGATTTTAATCTCAAAGAGACTTTTGA
AAGTGGCCAGTGCTTTAGATGGAATGAGGAAGAGGATGGAAGTTACACGGGGGTAGCGTATGACAGAGTTGTAAATGTGA
AGCTGGAAGGGGACACGCTAATCATTGACAATACCAATTTGACCGATTTTTACGATATATGGTTCGATTATTTTGACTTA
GGTAGAGATTACGGACAAATAAAGGAAAGTCTCTCAAAGGACCCTGTTCTCAAAGAAGCGATTAAATTTGGGCAAGGGAT
TAGGATTTTAAGACAGGATACTTGGGAAACGCTAGTTTCCTTCATAGTGTCCCAGAATAATAGAATCCCCCAAATAAAAA
AGGTAATAGAAAATTTGGCGACTAGTTTTGGAAATCCAATAGAGTATAAAGGTAAGATTTATTACACTTTTCCAAAACCT
GAAGAGCTTGTGATGTACGATGTAGAAACTATTGCAAAGACCAGATGCGGTTTTAGGGCAAAGTACATTTTTGATGCTGC
TTCCAAAGTCTTTTCGGGTGAGATCAACCTTTTAAAATTGCATGAATACAGCACCAGTGAAATAAGGGATATCTTGATGA
CTATAAACGGTGTGGGACCTAAAGTTGCCGATTGTGTAATTCTTTACTCTATCGGTCGTTATGATACTTTCCCCACTGAT
GTCTGGATAAAGAGAATAGTTGAGCATCTTTACCTGAAAAGGGAGGGTACCCCTGTAGAAATTCAGCTTTTTGCTATTGA
CAAATTTGGAGACCTCTCTGGTTTTGCTCAACAGTATTTGTTCTACTATGGGAGAGAGATGGGGAAGAAAATATTTGGGG
AAAGGAAGAAGTAA

Upstream 100 bases:

>100_bases
TCGGCTTTTTTTTGTACCAAAAAAATTATATAATAATAAATAACAGCAGGGAGAAATATGATAAGTAGTAAGTGAATATA
AACTCATGTGGGTGAGGAGA

Downstream 100 bases:

>100_bases
ATGCTGGGAACAATCGTAAATTCAATTGCGATAATCGTCGGAGGGACGATTGGAACTCTTCTTAAGGTAGGGATTCCAGA
TAGGTTTAAAAATATAGTTA

Product: 3-methyladenine DNA glycosylase

Products: NA

Alternate protein names: 8-oxoguanine DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase; AP lyase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKYNVEEKGTKVIVRGIADFNLKETFESGQCFRWNEEEDGSYTGVAYDRVVNVKLEGDTLIIDNTNLTDFYDIWFDYFDL
GRDYGQIKESLSKDPVLKEAIKFGQGIRILRQDTWETLVSFIVSQNNRIPQIKKVIENLATSFGNPIEYKGKIYYTFPKP
EELVMYDVETIAKTRCGFRAKYIFDAASKVFSGEINLLKLHEYSTSEIRDILMTINGVGPKVADCVILYSIGRYDTFPTD
VWIKRIVEHLYLKREGTPVEIQLFAIDKFGDLSGFAQQYLFYYGREMGKKIFGERKK

Sequences:

>Translated_297_residues
MKYNVEEKGTKVIVRGIADFNLKETFESGQCFRWNEEEDGSYTGVAYDRVVNVKLEGDTLIIDNTNLTDFYDIWFDYFDL
GRDYGQIKESLSKDPVLKEAIKFGQGIRILRQDTWETLVSFIVSQNNRIPQIKKVIENLATSFGNPIEYKGKIYYTFPKP
EELVMYDVETIAKTRCGFRAKYIFDAASKVFSGEINLLKLHEYSTSEIRDILMTINGVGPKVADCVILYSIGRYDTFPTD
VWIKRIVEHLYLKREGTPVEIQLFAIDKFGDLSGFAQQYLFYYGREMGKKIFGERKK
>Mature_297_residues
MKYNVEEKGTKVIVRGIADFNLKETFESGQCFRWNEEEDGSYTGVAYDRVVNVKLEGDTLIIDNTNLTDFYDIWFDYFDL
GRDYGQIKESLSKDPVLKEAIKFGQGIRILRQDTWETLVSFIVSQNNRIPQIKKVIENLATSFGNPIEYKGKIYYTFPKP
EELVMYDVETIAKTRCGFRAKYIFDAASKVFSGEINLLKLHEYSTSEIRDILMTINGVGPKVADCVILYSIGRYDTFPTD
VWIKRIVEHLYLKREGTPVEIQLFAIDKFGDLSGFAQQYLFYYGREMGKKIFGERKK

Specific function: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3' to the lesion [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-1 OGG1 family [H]

Homologues:

Organism=Homo sapiens, GI4505495, Length=289, Percent_Identity=29.757785467128, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI8670542, Length=249, Percent_Identity=30.9236947791165, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI8670540, Length=249, Percent_Identity=30.9236947791165, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI8670532, Length=249, Percent_Identity=30.9236947791165, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI8670530, Length=249, Percent_Identity=30.9236947791165, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI8670534, Length=249, Percent_Identity=30.9236947791165, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI8670536, Length=219, Percent_Identity=30.5936073059361, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323580, Length=214, Percent_Identity=36.9158878504673, Blast_Score=115, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24640654, Length=271, Percent_Identity=30.6273062730627, Blast_Score=98, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR004577
- InterPro:   IPR012904 [H]

Pfam domain/function: PF00730 HhH-GPD; PF07934 OGG_N [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 34453; Mature: 34453

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYNVEEKGTKVIVRGIADFNLKETFESGQCFRWNEEEDGSYTGVAYDRVVNVKLEGDTL
CCCCCCCCCCEEEEEECCCCCHHHHHCCCCCEECCCCCCCCEEEEEEEEEEEEEECCCEE
IIDNTNLTDFYDIWFDYFDLGRDYGQIKESLSKDPVLKEAIKFGQGIRILRQDTWETLVS
EEECCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
FIVSQNNRIPQIKKVIENLATSFGNPIEYKGKIYYTFPKPEELVMYDVETIAKTRCGFRA
HHHHCCCCCHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCEEEEEHHHHHHHHCCCCH
KYIFDAASKVFSGEINLLKLHEYSTSEIRDILMTINGVGPKVADCVILYSIGRYDTFPTD
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHEEEEEECCCCCCCCHH
VWIKRIVEHLYLKREGTPVEIQLFAIDKFGDLSGFAQQYLFYYGREMGKKIFGERKK
HHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKYNVEEKGTKVIVRGIADFNLKETFESGQCFRWNEEEDGSYTGVAYDRVVNVKLEGDTL
CCCCCCCCCCEEEEEECCCCCHHHHHCCCCCEECCCCCCCCEEEEEEEEEEEEEECCCEE
IIDNTNLTDFYDIWFDYFDLGRDYGQIKESLSKDPVLKEAIKFGQGIRILRQDTWETLVS
EEECCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
FIVSQNNRIPQIKKVIENLATSFGNPIEYKGKIYYTFPKPEELVMYDVETIAKTRCGFRA
HHHHCCCCCHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCEEEEEHHHHHHHHCCCCH
KYIFDAASKVFSGEINLLKLHEYSTSEIRDILMTINGVGPKVADCVILYSIGRYDTFPTD
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHEEEEEECCCCCCCCHH
VWIKRIVEHLYLKREGTPVEIQLFAIDKFGDLSGFAQQYLFYYGREMGKKIFGERKK
HHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]