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The map label for this gene is Gly1 [H]

Identifier: 20806788

GI number: 20806788

Start: 279529

End: 280569

Strand: Reverse

Name: Gly1 [H]

Synonym: TTE0267

Alternate gene names: 20806788

Gene position: 280569-279529 (Counterclockwise)

Preceding gene: 20806789

Following gene: 20806783

Centisome position: 10.43

GC content: 42.36

Gene sequence:

>1041_bases
ATGAAATATATTGACCTAAGAAGCGACACAGTAACCCAGCCCACTCCTGAGATGAGAGAAGCCATGTACAAAGCTGAAGT
GGGCGATGACGTATACGGAGAAGACCCTACTGTGAGAAAACTTGAAGAAATGGCAGCAGAAATGCTAGGAAAAGAAGCAG
CAATGCTTGTGACCAGCGGAACTCAAGGGAACCAAGTCTCAATAATGACTCACACTCATCCAGGAGAAGAAATCATACTA
GAGGAAAATTCCCACATAATAACCTATGAAGTTGGAGGGATAGGCTATCTCGCTGGAGTACAGGCAAAAACTTTGAAGGG
CAATAAAGGAGTTTTAAATCCGGAAGATGTGGAAAAAGCTATAAGGCCTAAAAACATACACTTTCCTGAGACAAGCCTAA
TCTGCCTTGAAAACACACACAATAGAGCAGGTGGCACTGTGACTCCAATTGAAGTCATGAAAAAGATATATGAAATCGCA
CAAAAGCATGGAATACCTGTACATCTGGACGGCGCCAGAATATTTAACGCCGCGACATATCTCAAAGTAGATGTAAAAGA
AATTGCGAAATATGCTGACAGCGTGATGTTCTGCCTTTCAAAAGGGTTATGTGCCCCTATCGGGTCTATCGTCGTCGGAA
CAAAAGAATTTATTGAAAGGGCGAGGAAATACCGCAAGATGCTGGGAGGAGGAATGAGGCAGGCAGGTTTCATCGCTGCT
GCTGGCATCGTAGCCCTTGAAAAAATGACAAAAAGGCTTCAAGAAGACCATGACAATGCAAGGCTTCTGGCCGAAGGTCT
GAAAAACATCCCCGGGATAGACCTTGATATGGAAACAGTGCAAACTAACATAGTAATGACTGATATTTCTGGCACTGGAA
TGACAGGAAGGGAATTTTCTCTAAAGTTAAAAGAACACGGCGTTTTAGTAAATGGGGGAGATGACTTTTCAGTAAGATTT
GTGACTCACTATTACATAACTAAAGAAGACGTAAAGAAGGCCTTGAGTGCAATTGAAAAAGTGGTAAGAGAAAAGCATTA
A

Upstream 100 bases:

>100_bases
TAGCAAAAAAATTAGGCATAAGCATTGCCACTTTATATAGAAAAATGGCAGAAGAGTGATACAATGAAGATAGAAAAACA
AAAAAGGGGGATATGGACAA

Downstream 100 bases:

>100_bases
AGAGAGGGTAACCCCCTCTCTTTTTAAGTATACTCTATCCTCACTTTGCTTCCCTCTCCTGAAACGGTAGGAGGCGTCAC
TATGAGCTTGACAGGGACCC

Product: threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKYIDLRSDTVTQPTPEMREAMYKAEVGDDVYGEDPTVRKLEEMAAEMLGKEAAMLVTSGTQGNQVSIMTHTHPGEEIIL
EENSHIITYEVGGIGYLAGVQAKTLKGNKGVLNPEDVEKAIRPKNIHFPETSLICLENTHNRAGGTVTPIEVMKKIYEIA
QKHGIPVHLDGARIFNAATYLKVDVKEIAKYADSVMFCLSKGLCAPIGSIVVGTKEFIERARKYRKMLGGGMRQAGFIAA
AGIVALEKMTKRLQEDHDNARLLAEGLKNIPGIDLDMETVQTNIVMTDISGTGMTGREFSLKLKEHGVLVNGGDDFSVRF
VTHYYITKEDVKKALSAIEKVVREKH

Sequences:

>Translated_346_residues
MKYIDLRSDTVTQPTPEMREAMYKAEVGDDVYGEDPTVRKLEEMAAEMLGKEAAMLVTSGTQGNQVSIMTHTHPGEEIIL
EENSHIITYEVGGIGYLAGVQAKTLKGNKGVLNPEDVEKAIRPKNIHFPETSLICLENTHNRAGGTVTPIEVMKKIYEIA
QKHGIPVHLDGARIFNAATYLKVDVKEIAKYADSVMFCLSKGLCAPIGSIVVGTKEFIERARKYRKMLGGGMRQAGFIAA
AGIVALEKMTKRLQEDHDNARLLAEGLKNIPGIDLDMETVQTNIVMTDISGTGMTGREFSLKLKEHGVLVNGGDDFSVRF
VTHYYITKEDVKKALSAIEKVVREKH
>Mature_346_residues
MKYIDLRSDTVTQPTPEMREAMYKAEVGDDVYGEDPTVRKLEEMAAEMLGKEAAMLVTSGTQGNQVSIMTHTHPGEEIIL
EENSHIITYEVGGIGYLAGVQAKTLKGNKGVLNPEDVEKAIRPKNIHFPETSLICLENTHNRAGGTVTPIEVMKKIYEIA
QKHGIPVHLDGARIFNAATYLKVDVKEIAKYADSVMFCLSKGLCAPIGSIVVGTKEFIERARKYRKMLGGGMRQAGFIAA
AGIVALEKMTKRLQEDHDNARLLAEGLKNIPGIDLDMETVQTNIVMTDISGTGMTGREFSLKLKEHGVLVNGGDDFSVRF
VTHYYITKEDVKKALSAIEKVVREKH

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=333, Percent_Identity=44.1441441441441, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI193206522, Length=347, Percent_Identity=42.6512968299712, Blast_Score=277, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI193206520, Length=347, Percent_Identity=42.6512968299712, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI193206524, Length=347, Percent_Identity=42.6512968299712, Blast_Score=276, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6320789, Length=348, Percent_Identity=36.7816091954023, Blast_Score=193, Evalue=4e-50,
Organism=Drosophila melanogaster, GI21355439, Length=312, Percent_Identity=46.474358974359, Blast_Score=280, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 38135; Mature: 38135

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIDLRSDTVTQPTPEMREAMYKAEVGDDVYGEDPTVRKLEEMAAEMLGKEAAMLVTSG
CCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECC
TQGNQVSIMTHTHPGEEIILEENSHIITYEVGGIGYLAGVQAKTLKGNKGVLNPEDVEKA
CCCCEEEEEEECCCCCEEEEECCCEEEEEEECCEEEECCCEEEEECCCCCCCCHHHHHHH
IRPKNIHFPETSLICLENTHNRAGGTVTPIEVMKKIYEIAQKHGIPVHLDGARIFNAATY
CCCCCCCCCCCEEEEEECCCCCCCCEECHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHE
LKVDVKEIAKYADSVMFCLSKGLCAPIGSIVVGTKEFIERARKYRKMLGGGMRQAGFIAA
EEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
AGIVALEKMTKRLQEDHDNARLLAEGLKNIPGIDLDMETVQTNIVMTDISGTGMTGREFS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHEEEEEEEECCCCCCCCCEEE
LKLKEHGVLVNGGDDFSVRFVTHYYITKEDVKKALSAIEKVVREKH
EEEECCCEEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYIDLRSDTVTQPTPEMREAMYKAEVGDDVYGEDPTVRKLEEMAAEMLGKEAAMLVTSG
CCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECC
TQGNQVSIMTHTHPGEEIILEENSHIITYEVGGIGYLAGVQAKTLKGNKGVLNPEDVEKA
CCCCEEEEEEECCCCCEEEEECCCEEEEEEECCEEEECCCEEEEECCCCCCCCHHHHHHH
IRPKNIHFPETSLICLENTHNRAGGTVTPIEVMKKIYEIAQKHGIPVHLDGARIFNAATY
CCCCCCCCCCCEEEEEECCCCCCCCEECHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHE
LKVDVKEIAKYADSVMFCLSKGLCAPIGSIVVGTKEFIERARKYRKMLGGGMRQAGFIAA
EEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
AGIVALEKMTKRLQEDHDNARLLAEGLKNIPGIDLDMETVQTNIVMTDISGTGMTGREFS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHEEEEEEEECCCCCCCCCEEE
LKLKEHGVLVNGGDDFSVRFVTHYYITKEDVKKALSAIEKVVREKH
EEEECCCEEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} 0.12 {DL-threo-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]