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The map label for this gene is PspF6 [H]

Identifier: 20806789

GI number: 20806789

Start: 280611

End: 282389

Strand: Reverse

Name: PspF6 [H]

Synonym: TTE0268

Alternate gene names: 20806789

Gene position: 282389-280611 (Counterclockwise)

Preceding gene: 20806791

Following gene: 20806788

Centisome position: 10.5

GC content: 36.48

Gene sequence:

>1779_bases
TTGTGGTATAGTATAATAAAAGCCCTTTTGCGTGGAGGGGAAATTATGGACAACATTTTAGAAGAAATAAAAAATGATTT
GCAGAGCATAGCAGAAACCATAAAAGCCATAACAAACGTAGATGTCACAATTGTGGACGAAAAATTGAAAAGAATTGCAG
GAACAGGTCCTTTTAAAGAAAAAATCGGAAGATACGCTCCAAAAAATTCCGCCTTTGAAAAAGCCTTAAAGACAGGGCGG
CAGTACTTCATAGTAAACCCTGGAAAGCACCCGATTTGCGCTTCCTGTGAGGAGCACCAAAATTGTAAAGAAGAGCTAGA
ACTTTGTATCCCCATAAGAAACGGCGAAAAAATAATAGGAATCCTGGGAATGTCCATCTTTGACAAAAATACAAAAATAG
ATTTCTTAAACAAACAGGAAGACTTCAAAAATTTTGAAAAAAGGTTCAGTGAACTCATAACTACGAAGATAAATGAGAAA
AAATTGGGCGTAATGATAGAATACCGCTCCTCAGAGCTCATGACTCTCATCAACTCCATAAACGAAGGAATAATAATTCT
TGACAGCCAAAACAGAGTGGTGACAGTAAACAAATATTTAAAGGAAAAGTTAAACGTTTCAGAGAAAACTCCTGTAAAGT
CTATTTTCCCCGTAAAACTGTTACAAGAATTAGAAAAGAAGGATTTTGAGGGAGAAGTAGGCCCTGTAGAGATTAAAGGA
ATAAAGTTTGTAATAAATTCTTCTCCTATTGCAGTAAAAGGACAAAGGGTGGGAACAGTTCTTGTATTTTCTGACTTTTC
AAAAATGCAGGAATCCGTCTACAAAGCTTCTAAAATGTCTGAAATTGTGACTTTTGACGATATAGTAGGAGAAAGTGACG
CAATAAAGTACGCAAAGAGTCAGGCAATTCAAATTGCGGATTCAGAGGTCCCTGTGCTTCTTTTGGGAGAAAGTGGAACA
GGAAAAGAATTGTTTGCCAGAGCAATACATTTTCAAAGCAGTCGCAAAAATGAGATATTCATGCCCATAAACTGCGGTGC
CATCCCCGAAACTTTAATAGAAAGCGAGCTTTTCGGGTATGAAAAGGGGTCCTTCACAGGAGCAAATGTGACAGGGAAAA
TTGGGAAATTTGAGCTTGCAAAGGACGGTACGATCTTTCTCGATGAAATAGGGGACTTACCCCTTTCTATGCAGGTAAAA
CTTCTGAGAGCCTTAGAAGAAAAAGAAATTGTAAGAATAGGTGGATATACCCCAATAAAAGTAAACCCTCGAATAATCTC
TGCCACTCATAAAGACCTGTATCAGATGGTTTTAGAGGGAAAATTTAGAGAAGACCTATTTTACCGGTTAAATGTAGTAC
CTATTGTAATTCCGCCTCTCCGAGAAAGAGGGTATGACATAATTATACTGGCAAACTATTTTCTAGAAAAGTTTAATAAA
ATCTATGGAAAAAATATAAAAGGCTTTTCGAAAGAAAGTGAGAAGTTGCTTTTAAGTTATCCTTTCCCTGGAAATATAAG
AGAGCTCAGAAACTTGATAGAGTATGCAGTTAACTTTGAAAGCAAGGACTATATATCACCCGAAACAATAAATCAGAGGC
TTCACAGAAAAACACTTGAAGAAGGCAAGACACTTGCTGAAATGGTAAAAAATTATGAAAAGAATGTGATAAAAGAGTAC
ATGTTAAGGTACGGGAGTGACGTTGAAAGCAAAAAACTGATAGCAAAAAAATTAGGCATAAGCATTGCCACTTTATATAG
AAAAATGGCAGAAGAGTGA

Upstream 100 bases:

>100_bases
CATTTCTGATAACAGTAAACGTTTTATAGTTTTTTATTTCTTACTGCAACGCCTTTTTTACTTCTCAATTATGAAAAATC
CTTATCACTTTTGATAAAGA

Downstream 100 bases:

>100_bases
TACAATGAAGATAGAAAAACAAAAAAGGGGGATATGGACAAATGAAATATATTGACCTAAGAAGCGACACAGTAACCCAG
CCCACTCCTGAGATGAGAGA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MWYSIIKALLRGGEIMDNILEEIKNDLQSIAETIKAITNVDVTIVDEKLKRIAGTGPFKEKIGRYAPKNSAFEKALKTGR
QYFIVNPGKHPICASCEEHQNCKEELELCIPIRNGEKIIGILGMSIFDKNTKIDFLNKQEDFKNFEKRFSELITTKINEK
KLGVMIEYRSSELMTLINSINEGIIILDSQNRVVTVNKYLKEKLNVSEKTPVKSIFPVKLLQELEKKDFEGEVGPVEIKG
IKFVINSSPIAVKGQRVGTVLVFSDFSKMQESVYKASKMSEIVTFDDIVGESDAIKYAKSQAIQIADSEVPVLLLGESGT
GKELFARAIHFQSSRKNEIFMPINCGAIPETLIESELFGYEKGSFTGANVTGKIGKFELAKDGTIFLDEIGDLPLSMQVK
LLRALEEKEIVRIGGYTPIKVNPRIISATHKDLYQMVLEGKFREDLFYRLNVVPIVIPPLRERGYDIIILANYFLEKFNK
IYGKNIKGFSKESEKLLLSYPFPGNIRELRNLIEYAVNFESKDYISPETINQRLHRKTLEEGKTLAEMVKNYEKNVIKEY
MLRYGSDVESKKLIAKKLGISIATLYRKMAEE

Sequences:

>Translated_592_residues
MWYSIIKALLRGGEIMDNILEEIKNDLQSIAETIKAITNVDVTIVDEKLKRIAGTGPFKEKIGRYAPKNSAFEKALKTGR
QYFIVNPGKHPICASCEEHQNCKEELELCIPIRNGEKIIGILGMSIFDKNTKIDFLNKQEDFKNFEKRFSELITTKINEK
KLGVMIEYRSSELMTLINSINEGIIILDSQNRVVTVNKYLKEKLNVSEKTPVKSIFPVKLLQELEKKDFEGEVGPVEIKG
IKFVINSSPIAVKGQRVGTVLVFSDFSKMQESVYKASKMSEIVTFDDIVGESDAIKYAKSQAIQIADSEVPVLLLGESGT
GKELFARAIHFQSSRKNEIFMPINCGAIPETLIESELFGYEKGSFTGANVTGKIGKFELAKDGTIFLDEIGDLPLSMQVK
LLRALEEKEIVRIGGYTPIKVNPRIISATHKDLYQMVLEGKFREDLFYRLNVVPIVIPPLRERGYDIIILANYFLEKFNK
IYGKNIKGFSKESEKLLLSYPFPGNIRELRNLIEYAVNFESKDYISPETINQRLHRKTLEEGKTLAEMVKNYEKNVIKEY
MLRYGSDVESKKLIAKKLGISIATLYRKMAEE
>Mature_592_residues
MWYSIIKALLRGGEIMDNILEEIKNDLQSIAETIKAITNVDVTIVDEKLKRIAGTGPFKEKIGRYAPKNSAFEKALKTGR
QYFIVNPGKHPICASCEEHQNCKEELELCIPIRNGEKIIGILGMSIFDKNTKIDFLNKQEDFKNFEKRFSELITTKINEK
KLGVMIEYRSSELMTLINSINEGIIILDSQNRVVTVNKYLKEKLNVSEKTPVKSIFPVKLLQELEKKDFEGEVGPVEIKG
IKFVINSSPIAVKGQRVGTVLVFSDFSKMQESVYKASKMSEIVTFDDIVGESDAIKYAKSQAIQIADSEVPVLLLGESGT
GKELFARAIHFQSSRKNEIFMPINCGAIPETLIESELFGYEKGSFTGANVTGKIGKFELAKDGTIFLDEIGDLPLSMQVK
LLRALEEKEIVRIGGYTPIKVNPRIISATHKDLYQMVLEGKFREDLFYRLNVVPIVIPPLRERGYDIIILANYFLEKFNK
IYGKNIKGFSKESEKLLLSYPFPGNIRELRNLIEYAVNFESKDYISPETINQRLHRKTLEEGKTLAEMVKNYEKNVIKEY
MLRYGSDVESKKLIAKKLGISIATLYRKMAEE

Specific function: Unknown

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789233, Length=604, Percent_Identity=35.4304635761589, Blast_Score=341, Evalue=6e-95,
Organism=Escherichia coli, GI1788550, Length=322, Percent_Identity=44.7204968944099, Blast_Score=256, Evalue=4e-69,
Organism=Escherichia coli, GI87082117, Length=266, Percent_Identity=47.7443609022556, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1790437, Length=337, Percent_Identity=41.5430267062315, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1786524, Length=399, Percent_Identity=34.8370927318296, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI1789087, Length=305, Percent_Identity=41.6393442622951, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1788905, Length=266, Percent_Identity=45.8646616541353, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1790299, Length=229, Percent_Identity=50.6550218340611, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI87082152, Length=237, Percent_Identity=46.8354430379747, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI87081872, Length=264, Percent_Identity=41.6666666666667, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787583, Length=426, Percent_Identity=30.2816901408451, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1789828, Length=238, Percent_Identity=33.1932773109244, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081858, Length=434, Percent_Identity=24.4239631336406, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 67246; Mature: 67246

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWYSIIKALLRGGEIMDNILEEIKNDLQSIAETIKAITNVDVTIVDEKLKRIAGTGPFKE
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCHHH
KIGRYAPKNSAFEKALKTGRQYFIVNPGKHPICASCEEHQNCKEELELCIPIRNGEKIIG
HHCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHHCCHHHHHHEEEEECCCCEEEE
ILGMSIFDKNTKIDFLNKQEDFKNFEKRFSELITTKINEKKLGVMIEYRSSELMTLINSI
EECHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHC
NEGIIILDSQNRVVTVNKYLKEKLNVSEKTPVKSIFPVKLLQELEKKDFEGEVGPVEIKG
CCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IKFVINSSPIAVKGQRVGTVLVFSDFSKMQESVYKASKMSEIVTFDDIVGESDAIKYAKS
EEEEECCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QAIQIADSEVPVLLLGESGTGKELFARAIHFQSSRKNEIFMPINCGAIPETLIESELFGY
HHEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCC
EKGSFTGANVTGKIGKFELAKDGTIFLDEIGDLPLSMQVKLLRALEEKEIVRIGGYTPIK
CCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCEEE
VNPRIISATHKDLYQMVLEGKFREDLFYRLNVVPIVIPPLRERGYDIIILANYFLEKFNK
ECCEEEEHHHHHHHHHHHCCCHHHHHHHEEEEEEEEECCHHHCCCEEEEEHHHHHHHHHH
IYGKNIKGFSKESEKLLLSYPFPGNIRELRNLIEYAVNFESKDYISPETINQRLHRKTLE
HHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
EGKTLAEMVKNYEKNVIKEYMLRYGSDVESKKLIAKKLGISIATLYRKMAEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHCC
>Mature Secondary Structure
MWYSIIKALLRGGEIMDNILEEIKNDLQSIAETIKAITNVDVTIVDEKLKRIAGTGPFKE
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCHHH
KIGRYAPKNSAFEKALKTGRQYFIVNPGKHPICASCEEHQNCKEELELCIPIRNGEKIIG
HHCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHHCCHHHHHHEEEEECCCCEEEE
ILGMSIFDKNTKIDFLNKQEDFKNFEKRFSELITTKINEKKLGVMIEYRSSELMTLINSI
EECHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHC
NEGIIILDSQNRVVTVNKYLKEKLNVSEKTPVKSIFPVKLLQELEKKDFEGEVGPVEIKG
CCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IKFVINSSPIAVKGQRVGTVLVFSDFSKMQESVYKASKMSEIVTFDDIVGESDAIKYAKS
EEEEECCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QAIQIADSEVPVLLLGESGTGKELFARAIHFQSSRKNEIFMPINCGAIPETLIESELFGY
HHEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCC
EKGSFTGANVTGKIGKFELAKDGTIFLDEIGDLPLSMQVKLLRALEEKEIVRIGGYTPIK
CCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCEEE
VNPRIISATHKDLYQMVLEGKFREDLFYRLNVVPIVIPPLRERGYDIIILANYFLEKFNK
ECCEEEEHHHHHHHHHHHCCCHHHHHHHEEEEEEEEECCHHHCCCEEEEEHHHHHHHHHH
IYGKNIKGFSKESEKLLLSYPFPGNIRELRNLIEYAVNFESKDYISPETINQRLHRKTLE
HHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
EGKTLAEMVKNYEKNVIKEYMLRYGSDVESKKLIAKKLGISIATLYRKMAEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]