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The map label for this gene is MurI

Identifier: 20806735

GI number: 20806735

Start: 215459

End: 216259

Strand: Direct

Name: MurI

Synonym: TTE0209

Alternate gene names: 20806735

Gene position: 215459-216259 (Clockwise)

Preceding gene: 20806734

Following gene: 20806736

Centisome position: 8.01

GC content: 32.71

Gene sequence:

>801_bases
ATGGATAAAAATGGACCTATAGGAATTATTGATTCAGGAGTCGGAGGAATTACAGTTTTAAAAAGACTTTTGGAAATTTT
GCCAGAGGAAGATTACATATATTTCGGAGATACTTTAAGGGTTCCTTACGGCAATAGACCAAAAGAAGAAATAGAAAAGT
TTACACGAGAAATTGTGAATTACTTGAAGAAGCAAAAAGTCAAAGCTGTAGTGATTGCTTGTAATACTATATGTTCAAGT
ATTAATAAGAATGACTATGAAATTTTGATGTTTGGAATATTAGAAGCAGGAATAAAGAGTGCAGTGGAAGCTACTTCAAA
TGGCCGAATAGGGGTAATAGCTACTAAAAGGACTGTAGAAAGTGAAGCTTATCTAAAGGGGATAAAAAGATTAAATAGAA
ACGCGATGGTTTTCCAAAAGGCTTGTCCGGAGCTGGTTTTAATTGTAGAAAATGGGTTTTATGAGGCATCTTCGATTTAC
TCAGCTGTAAAAAAATGTACTGAAGAATTTTTAGAGAAAGATATAGATACTCTAGTGCTTGGTTGTACTCATTTCCCTAT
TTTGCTTCCCTTTATTGAAAGAGTAGTAGATAATAAAGTGACTGTAGTAGATCCAGCAATAAAATTAGCTCATGAAGTAA
AAAAATATTTGGTAGAAAACAATTTGGTTAAGGACAAAAAAGGAGGACAAATTCGATTTTTAGTTACAGGTGAAAAGGAA
AATTTTATAAAGGTCGCTGGAACATTTCTCAACGACAAACATATAGATGTTTTAAGAATCGCTATAGAGGAACTGAAATA
G

Upstream 100 bases:

>100_bases
TATTAAAGTCTGATGATATAAAATTGGATTAAATACTTAGATGGGGGGTGAAAAGTAGAATAAAGTGCAGAAAAAATATT
TTAAGCAAAAGGTGGTGGAA

Downstream 100 bases:

>100_bases
GGATTTAAGGGGGAATAAAAAATGTTTTACAACTCTATAAAAGTAAATTCCAAGGGTCATTTAGTTATAGGTGGTTGTGA
TGCAGTAGAACTTGCTGAGA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MDKNGPIGIIDSGVGGITVLKRLLEILPEEDYIYFGDTLRVPYGNRPKEEIEKFTREIVNYLKKQKVKAVVIACNTICSS
INKNDYEILMFGILEAGIKSAVEATSNGRIGVIATKRTVESEAYLKGIKRLNRNAMVFQKACPELVLIVENGFYEASSIY
SAVKKCTEEFLEKDIDTLVLGCTHFPILLPFIERVVDNKVTVVDPAIKLAHEVKKYLVENNLVKDKKGGQIRFLVTGEKE
NFIKVAGTFLNDKHIDVLRIAIEELK

Sequences:

>Translated_266_residues
MDKNGPIGIIDSGVGGITVLKRLLEILPEEDYIYFGDTLRVPYGNRPKEEIEKFTREIVNYLKKQKVKAVVIACNTICSS
INKNDYEILMFGILEAGIKSAVEATSNGRIGVIATKRTVESEAYLKGIKRLNRNAMVFQKACPELVLIVENGFYEASSIY
SAVKKCTEEFLEKDIDTLVLGCTHFPILLPFIERVVDNKVTVVDPAIKLAHEVKKYLVENNLVKDKKGGQIRFLVTGEKE
NFIKVAGTFLNDKHIDVLRIAIEELK
>Mature_266_residues
MDKNGPIGIIDSGVGGITVLKRLLEILPEEDYIYFGDTLRVPYGNRPKEEIEKFTREIVNYLKKQKVKAVVIACNTICSS
INKNDYEILMFGILEAGIKSAVEATSNGRIGVIATKRTVESEAYLKGIKRLNRNAMVFQKACPELVLIVENGFYEASSIY
SAVKKCTEEFLEKDIDTLVLGCTHFPILLPFIERVVDNKVTVVDPAIKLAHEVKKYLVENNLVKDKKGGQIRFLVTGEKE
NFIKVAGTFLNDKHIDVLRIAIEELK

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=185, Percent_Identity=30.8108108108108, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI1_THETN (Q8RD38)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_621906.1
- ProteinModelPortal:   Q8RD38
- SMR:   Q8RD38
- GeneID:   997014
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0209
- NMPDR:   fig|273068.3.peg.820
- HOGENOM:   HBG645102
- OMA:   NSASAHA
- ProtClustDB:   CLSK900684
- BioCyc:   TTEN273068:TTE0209-MONOMER
- BRENDA:   5.1.1.3
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 29868; Mature: 29868

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKNGPIGIIDSGVGGITVLKRLLEILPEEDYIYFGDTLRVPYGNRPKEEIEKFTREIVN
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCHHHHHHHHHHHHH
YLKKQKVKAVVIACNTICSSINKNDYEILMFGILEAGIKSAVEATSNGRIGVIATKRTVE
HHHHHHHHHHEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHH
SEAYLKGIKRLNRNAMVFQKACPELVLIVENGFYEASSIYSAVKKCTEEFLEKDIDTLVL
HHHHHHHHHHHCCCHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GCTHFPILLPFIERVVDNKVTVVDPAIKLAHEVKKYLVENNLVKDKKGGQIRFLVTGEKE
HHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
NFIKVAGTFLNDKHIDVLRIAIEELK
CEEEEEEHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDKNGPIGIIDSGVGGITVLKRLLEILPEEDYIYFGDTLRVPYGNRPKEEIEKFTREIVN
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCHHHHHHHHHHHHH
YLKKQKVKAVVIACNTICSSINKNDYEILMFGILEAGIKSAVEATSNGRIGVIATKRTVE
HHHHHHHHHHEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHH
SEAYLKGIKRLNRNAMVFQKACPELVLIVENGFYEASSIYSAVKKCTEEFLEKDIDTLVL
HHHHHHHHHHHCCCHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GCTHFPILLPFIERVVDNKVTVVDPAIKLAHEVKKYLVENNLVKDKKGGQIRFLVTGEKE
HHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
NFIKVAGTFLNDKHIDVLRIAIEELK
CEEEEEEHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336