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The map label for this gene is manX [H]

Identifier: 20806719

GI number: 20806719

Start: 198848

End: 199351

Strand: Direct

Name: manX [H]

Synonym: TTE0193

Alternate gene names: 20806719

Gene position: 198848-199351 (Clockwise)

Preceding gene: 20806718

Following gene: 20806720

Centisome position: 7.39

GC content: 34.13

Gene sequence:

>504_bases
ATGTGGAAAATAGTTTTTGCTCGCATTGATGATAGACTGATCCATGGACAGGTAATGACAAGATGGATGAAAGGCTTTCC
TGAGGCTTCTATAGTGATTATTGATGATGAGTTGGCTGTAGATGAGTTTATGAAAAACATATATACTATGGCTGCTCCTC
CAGGAGTAAAAGTAAAAGTTTTCGGAGTAGATGCTGCTTTAAAAGAGTGGTCTCAAAAAACCAGTGTAGAAGAGAAAGTA
TTTTTACTTTTTAAGAATATTGATACATGTAAAAGAGTGATGGATGGAGGTCTTCCTATAACCACGTTGAATATAGGAGG
GGTTGCGAAAACTCCTCAGAGAAAAGGAATATCTCAAAGTGTTAGTCTGAGTGAAGATGAAGTGAAAACATTGCTCGAGC
TTAAAACAAAATATAATGTAGATGTATATTTACAAATGATACCTGATAGTGAAAAAATACATTTAACTACAGTGGTAGAA
AAATATTTTCCGGAATTGAAATAA

Upstream 100 bases:

>100_bases
ATACAACTGAATTACTTGAAAATATATCCAAAATAGGGAAGGATGGGATAAAAGTTATTGAAAAAAGTTCTCTTAAAATG
TAGAGAAAGGGGTCATGATG

Downstream 100 bases:

>100_bases
AGTTTCCGGTTTCTCTATGAAACCGGTACTATAAAATTTTTAAATTTTTTGAAAAGGGGTGAAAGTTTTATGGCAATCAG
TGCTTTGCAGGCTGCATTGA

Product: phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MWKIVFARIDDRLIHGQVMTRWMKGFPEASIVIIDDELAVDEFMKNIYTMAAPPGVKVKVFGVDAALKEWSQKTSVEEKV
FLLFKNIDTCKRVMDGGLPITTLNIGGVAKTPQRKGISQSVSLSEDEVKTLLELKTKYNVDVYLQMIPDSEKIHLTTVVE
KYFPELK

Sequences:

>Translated_167_residues
MWKIVFARIDDRLIHGQVMTRWMKGFPEASIVIIDDELAVDEFMKNIYTMAAPPGVKVKVFGVDAALKEWSQKTSVEEKV
FLLFKNIDTCKRVMDGGLPITTLNIGGVAKTPQRKGISQSVSLSEDEVKTLLELKTKYNVDVYLQMIPDSEKIHLTTVVE
KYFPELK
>Mature_167_residues
MWKIVFARIDDRLIHGQVMTRWMKGFPEASIVIIDDELAVDEFMKNIYTMAAPPGVKVKVFGVDAALKEWSQKTSVEEKV
FLLFKNIDTCKRVMDGGLPITTLNIGGVAKTPQRKGISQSVSLSEDEVKTLLELKTKYNVDVYLQMIPDSEKIHLTTVVE
KYFPELK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=158, Percent_Identity=32.9113924050633, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI87082217, Length=156, Percent_Identity=28.2051282051282, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 18966; Mature: 18966

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKIVFARIDDRLIHGQVMTRWMKGFPEASIVIIDDELAVDEFMKNIYTMAAPPGVKVKV
CEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEE
FGVDAALKEWSQKTSVEEKVFLLFKNIDTCKRVMDGGLPITTLNIGGVAKTPQRKGISQS
EECCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCC
VSLSEDEVKTLLELKTKYNVDVYLQMIPDSEKIHLTTVVEKYFPELK
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEHHHHHHHHCCCCC
>Mature Secondary Structure
MWKIVFARIDDRLIHGQVMTRWMKGFPEASIVIIDDELAVDEFMKNIYTMAAPPGVKVKV
CEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEE
FGVDAALKEWSQKTSVEEKVFLLFKNIDTCKRVMDGGLPITTLNIGGVAKTPQRKGISQS
EECCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCC
VSLSEDEVKTLLELKTKYNVDVYLQMIPDSEKIHLTTVVEKYFPELK
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]