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The map label for this gene is manZ [H]

Identifier: 20806720

GI number: 20806720

Start: 199421

End: 201085

Strand: Direct

Name: manZ [H]

Synonym: TTE0194

Alternate gene names: 20806720

Gene position: 199421-201085 (Clockwise)

Preceding gene: 20806719

Following gene: 20806721

Centisome position: 7.41

GC content: 38.5

Gene sequence:

>1665_bases
ATGGCAATCAGTGCTTTGCAGGCTGCATTGATAGCTGCATGGGTCGCAATCGTTGAGTCAAGAATCATAGGATATACGCT
TTCAGGATTTCTGCGTTTTACCGCTGTAGGAACTGGACTAGTTGTAGGATTGATAATGGGGAATGTGCCTCAAGCAATGA
TTATAGCAGCAACTATTCAGTTGGTTTACATGGGTGTTATCGCTCCAGGAGGTACTACTCCTAGTGAGACAGTTGTAGCA
ACTGCTGTAGCAGTTCCAGTTGCTTTGGCTTCTGGAATGGCTCCTGAGCAATCTATAGCAGTAGCGGTTCCTGTAGGTCT
TTTAGCATCATATTTAGTAAGTTTTAGATTCTTTATAAATTCTCTTATTACACACTGGGTTGATAGATTAGCAGAAGAGC
TAAATGACAGAGGAATCACATTTGCTTCTATCATTCTCCCTACTATTATTACGATATCACTGTTCTTCCCGTCTGTATTT
GTGGCAGTATATTATGGTGCTCCGTTGATAGATTCGTTTTTGAAGACTATACCAGCGAGCGTATTACATGCTTTAACGGT
TGTTGGAGGAAGTTTACCTGCTCTCGGTATTGCGCTTACTTTAGCAGTTATTGGGCGTAGAGACTTAATGGTATTCTATT
TCTTAGCATATTTTGTCTCATTAATTTTGAAACCTTTAAATGTAAATGCTCTGGTATATGCTATCTTAGGTGGTATTGCA
GCTTATCTATACGTTGCACTGTATTATAGAAGTGCGCTTGAAGAAAATAATGCAAATAGTGAAAATGTTTCATCAGGTGA
AGAGCCTCAATATGAAAAAGAAAGTTTACCTCAAAATGAGCTAAAGGTCACTGAAAAAGATTTAAAAATGACATGGTTTA
GATGGTGGTTAACAGTAGAAGTACCTCATTCTTTTGAAAGACTTCAGGCTTTGTCTTTCCAGTGGGCTATAATGCCTGTG
TTAAGGAAACTTTATGGGAAAAATCCAGAAGAACTGAAAGAAGCTTATAAGAGACATTTAGTATTTTTCAATACCCAGGG
CACATGGGGTGGCGCTCCTATTTTAGGTATCACCCTTTCTTTAGAAGAACAAAGAGCTAGAGCTATTGCAAAGGGGGAAG
AAGCACCTGATCCTAATATTATAAATGCGACAAAAGTTGGCTTAATGGGTCCATTAGCAGGTATAGGTGATTCAGTAGAA
TGGTCAACTATCATGTACTTGCTAATAGCAATAGCTCTTCCCTGGGCAAAATCAGGAAGTGCAATGGGAGCTCTGTTCCC
CTTGATACTTTTCCCTGTAATAACTTACGCGTATGGTTATTATTTCTTAAGAATGGGTTACAGATTAGGAAGAGAGTCAG
TAAGTGTATTGTTAGGTGGAGAAAAGATAAAAACACTCATTACAGGGTTGTCAACCTTGGGTCTTTTCATGATGGGTATT
TTGACGAGCAATTACGTTTCTGTGTCAACTCCGCTTAAATGGACCATTTCAGGTAAAGTGTTTGAATTACAAGCTATACT
AGACAAAATTTTACCTGGTATGCTGCCATTGCTGACTGTATTATTGGTATACTGGTACTTTACAAAGAAAGGGTTAAAGG
TTTTGAAAGTACTAGGCTGGATGGTGGTTATATTCTTCATTTTAGGTTGGATAGGAGTTCTATAA

Upstream 100 bases:

>100_bases
GGTAGAAAAATATTTTCCGGAATTGAAATAAAGTTTCCGGTTTCTCTATGAAACCGGTACTATAAAATTTTTAAATTTTT
TGAAAAGGGGTGAAAGTTTT

Downstream 100 bases:

>100_bases
AAATATTGATTCCACCCTCTTGGGTGGAATCAATATTTTGCATTATATTACATTTATTACATTTAAAATATAAAAGGAGG
ATAGGACAAATGAATGATGT

Product: mannose-specific PTS enzyme IID

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MAISALQAALIAAWVAIVESRIIGYTLSGFLRFTAVGTGLVVGLIMGNVPQAMIIAATIQLVYMGVIAPGGTTPSETVVA
TAVAVPVALASGMAPEQSIAVAVPVGLLASYLVSFRFFINSLITHWVDRLAEELNDRGITFASIILPTIITISLFFPSVF
VAVYYGAPLIDSFLKTIPASVLHALTVVGGSLPALGIALTLAVIGRRDLMVFYFLAYFVSLILKPLNVNALVYAILGGIA
AYLYVALYYRSALEENNANSENVSSGEEPQYEKESLPQNELKVTEKDLKMTWFRWWLTVEVPHSFERLQALSFQWAIMPV
LRKLYGKNPEELKEAYKRHLVFFNTQGTWGGAPILGITLSLEEQRARAIAKGEEAPDPNIINATKVGLMGPLAGIGDSVE
WSTIMYLLIAIALPWAKSGSAMGALFPLILFPVITYAYGYYFLRMGYRLGRESVSVLLGGEKIKTLITGLSTLGLFMMGI
LTSNYVSVSTPLKWTISGKVFELQAILDKILPGMLPLLTVLLVYWYFTKKGLKVLKVLGWMVVIFFILGWIGVL

Sequences:

>Translated_554_residues
MAISALQAALIAAWVAIVESRIIGYTLSGFLRFTAVGTGLVVGLIMGNVPQAMIIAATIQLVYMGVIAPGGTTPSETVVA
TAVAVPVALASGMAPEQSIAVAVPVGLLASYLVSFRFFINSLITHWVDRLAEELNDRGITFASIILPTIITISLFFPSVF
VAVYYGAPLIDSFLKTIPASVLHALTVVGGSLPALGIALTLAVIGRRDLMVFYFLAYFVSLILKPLNVNALVYAILGGIA
AYLYVALYYRSALEENNANSENVSSGEEPQYEKESLPQNELKVTEKDLKMTWFRWWLTVEVPHSFERLQALSFQWAIMPV
LRKLYGKNPEELKEAYKRHLVFFNTQGTWGGAPILGITLSLEEQRARAIAKGEEAPDPNIINATKVGLMGPLAGIGDSVE
WSTIMYLLIAIALPWAKSGSAMGALFPLILFPVITYAYGYYFLRMGYRLGRESVSVLLGGEKIKTLITGLSTLGLFMMGI
LTSNYVSVSTPLKWTISGKVFELQAILDKILPGMLPLLTVLLVYWYFTKKGLKVLKVLGWMVVIFFILGWIGVL
>Mature_553_residues
AISALQAALIAAWVAIVESRIIGYTLSGFLRFTAVGTGLVVGLIMGNVPQAMIIAATIQLVYMGVIAPGGTTPSETVVAT
AVAVPVALASGMAPEQSIAVAVPVGLLASYLVSFRFFINSLITHWVDRLAEELNDRGITFASIILPTIITISLFFPSVFV
AVYYGAPLIDSFLKTIPASVLHALTVVGGSLPALGIALTLAVIGRRDLMVFYFLAYFVSLILKPLNVNALVYAILGGIAA
YLYVALYYRSALEENNANSENVSSGEEPQYEKESLPQNELKVTEKDLKMTWFRWWLTVEVPHSFERLQALSFQWAIMPVL
RKLYGKNPEELKEAYKRHLVFFNTQGTWGGAPILGITLSLEEQRARAIAKGEEAPDPNIINATKVGLMGPLAGIGDSVEW
STIMYLLIAIALPWAKSGSAMGALFPLILFPVITYAYGYYFLRMGYRLGRESVSVLLGGEKIKTLITGLSTLGLFMMGIL
TSNYVSVSTPLKWTISGKVFELQAILDKILPGMLPLLTVLLVYWYFTKKGLKVLKVLGWMVVIFFILGWIGVL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=279, Percent_Identity=40.5017921146953, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789529, Length=280, Percent_Identity=28.5714285714286, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789528, Length=273, Percent_Identity=24.5421245421245, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 60473; Mature: 60341

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS51106 PTS_EIIC_TYPE_4 ; PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISALQAALIAAWVAIVESRIIGYTLSGFLRFTAVGTGLVVGLIMGNVPQAMIIAATIQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LVYMGVIAPGGTTPSETVVATAVAVPVALASGMAPEQSIAVAVPVGLLASYLVSFRFFIN
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
SLITHWVDRLAEELNDRGITFASIILPTIITISLFFPSVFVAVYYGAPLIDSFLKTIPAS
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
VLHALTVVGGSLPALGIALTLAVIGRRDLMVFYFLAYFVSLILKPLNVNALVYAILGGIA
HHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AYLYVALYYRSALEENNANSENVSSGEEPQYEKESLPQNELKVTEKDLKMTWFRWWLTVE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHEEEEEEEEEE
VPHSFERLQALSFQWAIMPVLRKLYGKNPEELKEAYKRHLVFFNTQGTWGGAPILGITLS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEE
LEEQRARAIAKGEEAPDPNIINATKVGLMGPLAGIGDSVEWSTIMYLLIAIALPWAKSGS
EHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
AMGALFPLILFPVITYAYGYYFLRMGYRLGRESVSVLLGGEKIKTLITGLSTLGLFMMGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTSNYVSVSTPLKWTISGKVFELQAILDKILPGMLPLLTVLLVYWYFTKKGLKVLKVLGW
HHCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVVIFFILGWIGVL
HHHHHHHHHHHHCC
>Mature Secondary Structure 
AISALQAALIAAWVAIVESRIIGYTLSGFLRFTAVGTGLVVGLIMGNVPQAMIIAATIQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LVYMGVIAPGGTTPSETVVATAVAVPVALASGMAPEQSIAVAVPVGLLASYLVSFRFFIN
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
SLITHWVDRLAEELNDRGITFASIILPTIITISLFFPSVFVAVYYGAPLIDSFLKTIPAS
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
VLHALTVVGGSLPALGIALTLAVIGRRDLMVFYFLAYFVSLILKPLNVNALVYAILGGIA
HHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AYLYVALYYRSALEENNANSENVSSGEEPQYEKESLPQNELKVTEKDLKMTWFRWWLTVE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHEEEEEEEEEE
VPHSFERLQALSFQWAIMPVLRKLYGKNPEELKEAYKRHLVFFNTQGTWGGAPILGITLS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEE
LEEQRARAIAKGEEAPDPNIINATKVGLMGPLAGIGDSVEWSTIMYLLIAIALPWAKSGS
EHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
AMGALFPLILFPVITYAYGYYFLRMGYRLGRESVSVLLGGEKIKTLITGLSTLGLFMMGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTSNYVSVSTPLKWTISGKVFELQAILDKILPGMLPLLTVLLVYWYFTKKGLKVLKVLGW
HHCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVVIFFILGWIGVL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]