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The map label for this gene is RpiB [H]

Identifier: 20806675

GI number: 20806675

Start: 144754

End: 145662

Strand: Direct

Name: RpiB [H]

Synonym: TTE0146

Alternate gene names: 20806675

Gene position: 144754-145662 (Clockwise)

Preceding gene: 20806674

Following gene: 20806676

Centisome position: 5.38

GC content: 42.9

Gene sequence:

>909_bases
ATGAGAGTTTTATTTGTGTGTACAGGGAATACCTGTAGAAGCCCTATGGCTGAAGGGATTTTTAATGCTAAGTCAAAAGC
TTTGGGCAAGGATTGGGAAGCAAAATCTGCGGGAGTATTTGCTCCTGAAGGATTTCCAGCCTCAAGCGAGGCAGTAGAAG
TGTTAAAGAAAGAGTACGGGATAGATATTTCAGACCACAGGGCAAAAAGTTTGAGGGAAGAAGATTTAAAAGGTGCTGAT
TTAGTCCTTGCCATGGCTTTTTCCCACAAAAGAAGCTTGGTATCTCAATACCCTGAATATGCCGACAAAATTTTCACCAT
AAAAGAATTTGTTGGACTTGAAGGGGATGTAGAAGACCCTTACGGGATGCCTTTGGAGGTTTACAAAAAGACGGCAGAGG
AGCTTTCCGGTTTGATAGATAAGCTCATCGAAAAACTCTCTGAGGAAAGGAAGGTAGATCGAATGATCGCTTTGGGTTCT
GACCACGCAGGATATGAACTTAAAGAAGTGATAAAAAAATATTTAGAAGAGAAAGGGATACCCTACAAGGACTTTGGCAC
TTTTAATGAAGAGTCAGTGGACTATCCCGACTATGCTCTTAAAGTAGCTGAAGCTGTGGCGTCTGGAGAGTGCACAGAAG
GAATTTTAGTGTGCGGGACTGGCATAGGCATGTCAATTACGGCAAATAAAGTGCCAGGAATTAGAGCTGCCCACGTAGAG
GACGTGTTTTCAGCAAGGGCTGCCAAAGAACATAACAATGCCAATATCCTGTGCATGGGGGGAAGAGTTACAGGACCTGG
ACTTGCTATCATGATGGTAGAGGAGTGGCTAAATGCCACTTTCCAAGGAGGAAGGCATCAGAAGAGGATTGACAAAATAA
CAGAAATCGAAAAAAAGTACATGAAATGA

Upstream 100 bases:

>100_bases
TTGCTGAAAGCTTCAATTATGATGACATTGGACTTGCCATAATGAACAGGTTGGAAAAAGCTGCGGGGTATAAGGAAATA
AAGGCTGAAGGGGAGTTGAC

Downstream 100 bases:

>100_bases
TAAACAGGAGGGTTTTTATTTTATGTATGAGAATGTCTTTGTTATAGATCATCCTCTTATACAACATAAAATAAGTCTCA
TAAGAGATGAAAACACGGGT

Product: ribose 5-phosphate isomerase RpiB

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRVLFVCTGNTCRSPMAEGIFNAKSKALGKDWEAKSAGVFAPEGFPASSEAVEVLKKEYGIDISDHRAKSLREEDLKGAD
LVLAMAFSHKRSLVSQYPEYADKIFTIKEFVGLEGDVEDPYGMPLEVYKKTAEELSGLIDKLIEKLSEERKVDRMIALGS
DHAGYELKEVIKKYLEEKGIPYKDFGTFNEESVDYPDYALKVAEAVASGECTEGILVCGTGIGMSITANKVPGIRAAHVE
DVFSARAAKEHNNANILCMGGRVTGPGLAIMMVEEWLNATFQGGRHQKRIDKITEIEKKYMK

Sequences:

>Translated_302_residues
MRVLFVCTGNTCRSPMAEGIFNAKSKALGKDWEAKSAGVFAPEGFPASSEAVEVLKKEYGIDISDHRAKSLREEDLKGAD
LVLAMAFSHKRSLVSQYPEYADKIFTIKEFVGLEGDVEDPYGMPLEVYKKTAEELSGLIDKLIEKLSEERKVDRMIALGS
DHAGYELKEVIKKYLEEKGIPYKDFGTFNEESVDYPDYALKVAEAVASGECTEGILVCGTGIGMSITANKVPGIRAAHVE
DVFSARAAKEHNNANILCMGGRVTGPGLAIMMVEEWLNATFQGGRHQKRIDKITEIEKKYMK
>Mature_302_residues
MRVLFVCTGNTCRSPMAEGIFNAKSKALGKDWEAKSAGVFAPEGFPASSEAVEVLKKEYGIDISDHRAKSLREEDLKGAD
LVLAMAFSHKRSLVSQYPEYADKIFTIKEFVGLEGDVEDPYGMPLEVYKKTAEELSGLIDKLIEKLSEERKVDRMIALGS
DHAGYELKEVIKKYLEEKGIPYKDFGTFNEESVDYPDYALKVAEAVASGECTEGILVCGTGIGMSITANKVPGIRAAHVE
DVFSARAAKEHNNANILCMGGRVTGPGLAIMMVEEWLNATFQGGRHQKRIDKITEIEKKYMK

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=144, Percent_Identity=50, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 33333; Mature: 33333

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLFVCTGNTCRSPMAEGIFNAKSKALGKDWEAKSAGVFAPEGFPASSEAVEVLKKEYG
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
IDISDHRAKSLREEDLKGADLVLAMAFSHKRSLVSQYPEYADKIFTIKEFVGLEGDVEDP
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YGMPLEVYKKTAEELSGLIDKLIEKLSEERKVDRMIALGSDHAGYELKEVIKKYLEEKGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
PYKDFGTFNEESVDYPDYALKVAEAVASGECTEGILVCGTGIGMSITANKVPGIRAAHVE
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHH
DVFSARAAKEHNNANILCMGGRVTGPGLAIMMVEEWLNATFQGGRHQKRIDKITEIEKKY
HHHHHHHHHCCCCCCEEEECCEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MK
CC
>Mature Secondary Structure
MRVLFVCTGNTCRSPMAEGIFNAKSKALGKDWEAKSAGVFAPEGFPASSEAVEVLKKEYG
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
IDISDHRAKSLREEDLKGADLVLAMAFSHKRSLVSQYPEYADKIFTIKEFVGLEGDVEDP
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YGMPLEVYKKTAEELSGLIDKLIEKLSEERKVDRMIALGSDHAGYELKEVIKKYLEEKGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
PYKDFGTFNEESVDYPDYALKVAEAVASGECTEGILVCGTGIGMSITANKVPGIRAAHVE
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHH
DVFSARAAKEHNNANILCMGGRVTGPGLAIMMVEEWLNATFQGGRHQKRIDKITEIEKKY
HHHHHHHHHCCCCCCEEEECCEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]