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The map label for this gene is GcvH

Identifier: 20806618

GI number: 20806618

Start: 93677

End: 94054

Strand: Direct

Name: GcvH

Synonym: TTE0089

Alternate gene names: 20806618

Gene position: 93677-94054 (Clockwise)

Preceding gene: 20806617

Following gene: 20806619

Centisome position: 3.48

GC content: 39.68

Gene sequence:

>378_bases
ATGAAAGTTTTGGAAGATTTGTATTACTCAAAGGACCATGTATGGGTAAAAGTAGAAGGGGACAAAGCTTATATTGGCAT
AACTGATTATGCCCAGGACTCTTTAGGGGATGCGGAGTACATTGAACTTCCTGAAGTTGGAACAGAGTTTACAGCTGGAG
ATGTGCTTGGAGTTATAGAATCTGCAAAAGCGGCTTCTGACGTTTATATACCTGTAGACGGAGAAGTAATTGAAGTGAAC
AATGCTGTAGCTGAAGACCCTTCTTTGGTAAATAGCGATCCCTATGGAAGCTGGCTTGTTGCTGTAAGGTTGAAAGATAA
AGCTCAAGTTGAAAAGCTTATGAAGGCAGAAGAGTACAAAAAATTTTTGGAAACGTAA

Upstream 100 bases:

>100_bases
GCCCATCCTACAAAGAGCGAAACTATTATGGAGGCTTTTAAAGATATTTTCAAAGAGGCAATCCACAAAATTTAAATTAT
TAAAAATTGGAGGGACAAAA

Downstream 100 bases:

>100_bases
TGAAGGGCCCTTTACGGGCCTTTTTTAGTTTATTGAAATTTTCAATAATGGTATAATTAGTTTAAAAGGTTTTTAATGAG
GGAGGTAGTAAAGTTGAAGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MKVLEDLYYSKDHVWVKVEGDKAYIGITDYAQDSLGDAEYIELPEVGTEFTAGDVLGVIESAKAASDVYIPVDGEVIEVN
NAVAEDPSLVNSDPYGSWLVAVRLKDKAQVEKLMKAEEYKKFLET

Sequences:

>Translated_125_residues
MKVLEDLYYSKDHVWVKVEGDKAYIGITDYAQDSLGDAEYIELPEVGTEFTAGDVLGVIESAKAASDVYIPVDGEVIEVN
NAVAEDPSLVNSDPYGSWLVAVRLKDKAQVEKLMKAEEYKKFLET
>Mature_125_residues
MKVLEDLYYSKDHVWVKVEGDKAYIGITDYAQDSLGDAEYIELPEVGTEFTAGDVLGVIESAKAASDVYIPVDGEVIEVN
NAVAEDPSLVNSDPYGSWLVAVRLKDKAQVEKLMKAEEYKKFLET

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=41.5254237288136, Blast_Score=108, Evalue=9e-25,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=41.5254237288136, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=46.4, Blast_Score=120, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=44.3478260869565, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=40.8695652173913, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=52.5, Blast_Score=123, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=47.8991596638655, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH1_THETN (Q8RDF0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_621789.1
- ProteinModelPortal:   Q8RDF0
- SMR:   Q8RDF0
- GeneID:   996407
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0089
- NMPDR:   fig|273068.3.peg.262
- HOGENOM:   HBG693789
- OMA:   QGAGWFF
- ProtClustDB:   PRK01202
- BioCyc:   TTEN273068:TTE0089-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13852; Mature: 13852

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLEDLYYSKDHVWVKVEGDKAYIGITDYAQDSLGDAEYIELPEVGTEFTAGDVLGVIE
CCCHHHHHCCCCEEEEEEECCEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHH
SAKAASDVYIPVDGEVIEVNNAVAEDPSLVNSDPYGSWLVAVRLKDKAQVEKLMKAEEYK
CCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
KFLET
HHHCC
>Mature Secondary Structure
MKVLEDLYYSKDHVWVKVEGDKAYIGITDYAQDSLGDAEYIELPEVGTEFTAGDVLGVIE
CCCHHHHHCCCCEEEEEEECCEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHH
SAKAASDVYIPVDGEVIEVNNAVAEDPSLVNSDPYGSWLVAVRLKDKAQVEKLMKAEEYK
CCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
KFLET
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336