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The map label for this gene is Lpd [H]

Identifier: 20806617

GI number: 20806617

Start: 92266

End: 93651

Strand: Direct

Name: Lpd [H]

Synonym: TTE0088

Alternate gene names: 20806617

Gene position: 92266-93651 (Clockwise)

Preceding gene: 20806616

Following gene: 20806618

Centisome position: 3.43

GC content: 38.38

Gene sequence:

>1386_bases
ATGGAAAACCTTCATACCAGAGAATATGACTTGGCTGTCATAGGCGGAGGGCCTGGAGGGTATGTGGCGGCTATCAAAGC
TGCTAAAAAAGGGGCAAAAGTAGCTCTGTTTGAAAAGGATAAATTAGGGGGCACATGTCTTAATAGAGGTTGTATACCTA
CAAAGGCTTATGCAAGAGCTGCTGAGGTCTATGGAATTTTAAAAAAGGCAAAAGAGTTTGGCTTTGATATTCAAATAAAT
TATTTTGATTATGCGCAAGTAGTGAAGAGAAAGGATACCATTGTAGGGGAACTTGTTGAAGGCATTAAGGCTTTGCTTAA
AGCCAATAAAATAGAGGTCTTCAATAAAGAAGCGAAGGTAGACAAAGAGAAGAATGTAATTTTTGAGGGAGAAAAAATCA
AAGCGAAAAACATTATAATTGCTACTGGTTCATCTCCTGCAGAGCTTCCAATTGAAGGCATTGATTCGAAAAATGTTCTA
AATAGCGATACAATTTTGGAGATAACGTCTTTACCCAAGAGTTTATGCATTATAGGTGGCGGAGTAATAGGGATGGAATT
TGCTTTTATAATGAACCAATTTGGAGTAGAAGTGTATGTGGTTGAAATGATGCCTAATATCCTCCCTTCCCTTGATAAAA
AAGTAAGTTCTGCTGTTAAATTTGCTGCTCAGAAGAGAGGCATTAAGATTTATACTTCTTCTACTGTGGAAAAAGTAGAG
GAGGAGGGAGAAAACTCTGTAGTTACGATTAGAAGCGGTGATGATATTAAGAAGATATCTGTTGATAAAGTGTTTGTTTC
TATTGGAAGAAAGTTAAATACAAGTATAGGGCCAATTGTAGATTTGCTTGAATTTGATAAGAAAGCGATTAAAGTCGATG
AGCACATGAGGACAAACATAGAGGGTGTTTGGGCTGTAGGAGATGTGACAGGTAAAATGATGCTGGCCCATGTAGCTTCT
TCACAGGGAGAAGTGGCAGTTGACAATATTTTTGGAAAGTCGAGAACTTTGGATTACTACAAGATACCTGCAGCTGTTTT
TACAGAACCAGAAATTGGATATTTCGGGTATACAGAAGAGGAGGCCAAAGAAAAATTTGGAGAAATTAAAGTAGGAAGAT
TTGACTTCAAGCATAACGGGAGAGCCAAAACTTATGGTGAGACAGAAGGATTTGCCAAGATAATTTCAACTGAAGATGGA
GAAGTAGTTGGGGCATGGGTAGTAGGTAGTGGCGCTTCTGAGCTTGTGCACATAATTTCAACGGCTTGTCAGAGCGGAGC
AAAAGCGGAAGAATTGAAAGATGTGGTCTATGCCCATCCTACAAAGAGCGAAACTATTATGGAGGCTTTTAAAGATATTT
TCAAAGAGGCAATCCACAAAATTTAA

Upstream 100 bases:

>100_bases
CGAAGTGACTTTGGTGGATTATGATGGATGGGCGGAACTTATCGAGAAGGAGGAAAAAGTGATTTCTTAATTTACAAAAT
TTATATTGGAGGTAGAGTAA

Downstream 100 bases:

>100_bases
ATTATTAAAAATTGGAGGGACAAAAATGAAAGTTTTGGAAGATTTGTATTACTCAAAGGACCATGTATGGGTAAAAGTAG
AAGGGGACAAAGCTTATATT

Product: dihydrolipoamide dehydrogenase/glutathione oxidoreductase and related enzyme

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MENLHTREYDLAVIGGGPGGYVAAIKAAKKGAKVALFEKDKLGGTCLNRGCIPTKAYARAAEVYGILKKAKEFGFDIQIN
YFDYAQVVKRKDTIVGELVEGIKALLKANKIEVFNKEAKVDKEKNVIFEGEKIKAKNIIIATGSSPAELPIEGIDSKNVL
NSDTILEITSLPKSLCIIGGGVIGMEFAFIMNQFGVEVYVVEMMPNILPSLDKKVSSAVKFAAQKRGIKIYTSSTVEKVE
EEGENSVVTIRSGDDIKKISVDKVFVSIGRKLNTSIGPIVDLLEFDKKAIKVDEHMRTNIEGVWAVGDVTGKMMLAHVAS
SQGEVAVDNIFGKSRTLDYYKIPAAVFTEPEIGYFGYTEEEAKEKFGEIKVGRFDFKHNGRAKTYGETEGFAKIISTEDG
EVVGAWVVGSGASELVHIISTACQSGAKAEELKDVVYAHPTKSETIMEAFKDIFKEAIHKI

Sequences:

>Translated_461_residues
MENLHTREYDLAVIGGGPGGYVAAIKAAKKGAKVALFEKDKLGGTCLNRGCIPTKAYARAAEVYGILKKAKEFGFDIQIN
YFDYAQVVKRKDTIVGELVEGIKALLKANKIEVFNKEAKVDKEKNVIFEGEKIKAKNIIIATGSSPAELPIEGIDSKNVL
NSDTILEITSLPKSLCIIGGGVIGMEFAFIMNQFGVEVYVVEMMPNILPSLDKKVSSAVKFAAQKRGIKIYTSSTVEKVE
EEGENSVVTIRSGDDIKKISVDKVFVSIGRKLNTSIGPIVDLLEFDKKAIKVDEHMRTNIEGVWAVGDVTGKMMLAHVAS
SQGEVAVDNIFGKSRTLDYYKIPAAVFTEPEIGYFGYTEEEAKEKFGEIKVGRFDFKHNGRAKTYGETEGFAKIISTEDG
EVVGAWVVGSGASELVHIISTACQSGAKAEELKDVVYAHPTKSETIMEAFKDIFKEAIHKI
>Mature_461_residues
MENLHTREYDLAVIGGGPGGYVAAIKAAKKGAKVALFEKDKLGGTCLNRGCIPTKAYARAAEVYGILKKAKEFGFDIQIN
YFDYAQVVKRKDTIVGELVEGIKALLKANKIEVFNKEAKVDKEKNVIFEGEKIKAKNIIIATGSSPAELPIEGIDSKNVL
NSDTILEITSLPKSLCIIGGGVIGMEFAFIMNQFGVEVYVVEMMPNILPSLDKKVSSAVKFAAQKRGIKIYTSSTVEKVE
EEGENSVVTIRSGDDIKKISVDKVFVSIGRKLNTSIGPIVDLLEFDKKAIKVDEHMRTNIEGVWAVGDVTGKMMLAHVAS
SQGEVAVDNIFGKSRTLDYYKIPAAVFTEPEIGYFGYTEEEAKEKFGEIKVGRFDFKHNGRAKTYGETEGFAKIISTEDG
EVVGAWVVGSGASELVHIISTACQSGAKAEELKDVVYAHPTKSETIMEAFKDIFKEAIHKI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=34.9344978165939, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI50301238, Length=461, Percent_Identity=31.6702819956616, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI22035672, Length=477, Percent_Identity=28.7211740041929, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI148277065, Length=455, Percent_Identity=25.7142857142857, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI33519430, Length=455, Percent_Identity=25.7142857142857, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI33519428, Length=455, Percent_Identity=25.7142857142857, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI33519426, Length=455, Percent_Identity=25.7142857142857, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI148277071, Length=455, Percent_Identity=25.7142857142857, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI291045266, Length=436, Percent_Identity=27.0642201834862, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI291045268, Length=426, Percent_Identity=24.8826291079812, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI21389617, Length=300, Percent_Identity=24.6666666666667, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI226437568, Length=300, Percent_Identity=24.6666666666667, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI65787454, Length=300, Percent_Identity=24.6666666666667, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1786307, Length=459, Percent_Identity=31.5904139433551, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=28.8683602771363, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87082354, Length=453, Percent_Identity=28.2560706401766, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=28.4164859002169, Blast_Score=165, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=34.9892008639309, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI71983429, Length=456, Percent_Identity=28.0701754385965, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71983419, Length=456, Percent_Identity=28.0701754385965, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=30.168776371308, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=26.7893660531697, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=233, Percent_Identity=28.755364806867, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=35.3556485355649, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=29.7644539614561, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=28.9308176100629, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21358499, Length=462, Percent_Identity=35.2813852813853, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24640549, Length=476, Percent_Identity=26.6806722689076, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24640553, Length=476, Percent_Identity=26.6806722689076, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24640551, Length=476, Percent_Identity=26.4705882352941, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI17737741, Length=477, Percent_Identity=24.3186582809224, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50305; Mature: 50305

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLHTREYDLAVIGGGPGGYVAAIKAAKKGAKVALFEKDKLGGTCLNRGCIPTKAYARA
CCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCHHHHHHH
AEVYGILKKAKEFGFDIQINYFDYAQVVKRKDTIVGELVEGIKALLKANKIEVFNKEAKV
HHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHCC
DKEKNVIFEGEKIKAKNIIIATGSSPAELPIEGIDSKNVLNSDTILEITSLPKSLCIIGG
CCCCCEEEECCEEEECEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECC
GVIGMEFAFIMNQFGVEVYVVEMMPNILPSLDKKVSSAVKFAAQKRGIKIYTSSTVEKVE
CHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHH
EEGENSVVTIRSGDDIKKISVDKVFVSIGRKLNTSIGPIVDLLEFDKKAIKVDEHMRTNI
HCCCCCEEEEECCCCCEEHHHHHHHHHHCHHHCCCHHHHHHHHHHCHHHHHHHHHHHCCC
EGVWAVGDVTGKMMLAHVASSQGEVAVDNIFGKSRTLDYYKIPAAVFTEPEIGYFGYTEE
CEEEEECCHHHHHHHHHHHCCCCCEEEHHHCCCCCCCEEEECCHHEECCCCCCCCCCCHH
EAKEKFGEIKVGRFDFKHNGRAKTYGETEGFAKIISTEDGEVVGAWVVGSGASELVHIIS
HHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHEEEECCCCCEEEEEEECCCHHHHHHHHH
TACQSGAKAEELKDVVYAHPTKSETIMEAFKDIFKEAIHKI
HHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENLHTREYDLAVIGGGPGGYVAAIKAAKKGAKVALFEKDKLGGTCLNRGCIPTKAYARA
CCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCHHHHHHH
AEVYGILKKAKEFGFDIQINYFDYAQVVKRKDTIVGELVEGIKALLKANKIEVFNKEAKV
HHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHCC
DKEKNVIFEGEKIKAKNIIIATGSSPAELPIEGIDSKNVLNSDTILEITSLPKSLCIIGG
CCCCCEEEECCEEEECEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECC
GVIGMEFAFIMNQFGVEVYVVEMMPNILPSLDKKVSSAVKFAAQKRGIKIYTSSTVEKVE
CHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHH
EEGENSVVTIRSGDDIKKISVDKVFVSIGRKLNTSIGPIVDLLEFDKKAIKVDEHMRTNI
HCCCCCEEEEECCCCCEEHHHHHHHHHHCHHHCCCHHHHHHHHHHCHHHHHHHHHHHCCC
EGVWAVGDVTGKMMLAHVASSQGEVAVDNIFGKSRTLDYYKIPAAVFTEPEIGYFGYTEE
CEEEEECCHHHHHHHHHHHCCCCCEEEHHHCCCCCCCEEEECCHHEECCCCCCCCCCCHH
EAKEKFGEIKVGRFDFKHNGRAKTYGETEGFAKIISTEDGEVVGAWVVGSGASELVHIIS
HHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHEEEECCCCCEEEEEEECCCHHHHHHHHH
TACQSGAKAEELKDVVYAHPTKSETIMEAFKDIFKEAIHKI
HHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]