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The map label for this gene is DdlA [H]

Identifier: 20806600

GI number: 20806600

Start: 75957

End: 76955

Strand: Direct

Name: DdlA [H]

Synonym: TTE0068

Alternate gene names: 20806600

Gene position: 75957-76955 (Clockwise)

Preceding gene: 20806599

Following gene: 20806601

Centisome position: 2.82

GC content: 37.44

Gene sequence:

>999_bases
ATGAAAGTAGGTGTTTTTTGGAGAAGATTCAGAAATGTCGAGCTTCAAAGAAGACTTACACCTGATAAAGTCTATGACGA
TGCCTATGACGAAGCTTATCATCACTATGAAGCATTAAAAGAAGCAGGATTCGATGCAGTCCTCATAGAATGGAAAGATG
ACCCTATCAAAACTTACGAGGTCATTCAAAAAGAAGAAGTAGACATAATTTTTAATGCCTCTAGCTTGAAGGAAATAGAA
TTTTTGGAACTATTTAATATACCCTATGTAGGTTCTGGTATTGATTTGGCGGCAACAGATAAAAGGATGAGAAAAGACAT
TGTAGCTGCTCACGGTCTTCCCACCCCTAAATATGTGGTGGCATACAGTGCGAATGAAATTCCTTCTGTAGACCATTTAA
GGTTTCCGCTTTTTGTAAAACCTATAATGGGAAGAGGGAGTGCAGGGATAGATGAGGAAAATATAGTGTACGATAAGTCT
AGGCTTCCAAAGGTTGTGAGCAAGATAACTGAAAAGATAGGTCAGCCTGCTCTTATTGAAGAGTTTATTGAAGGAAGGGA
AGTAACCGTAGGGATAATAGGTTATAGGAATCCGATAGTATTGCCCCTTTTAGAGATAGGTTATAATAATGTCAAGACAA
ATACCTATGAACACAAAATGTTTGACAATGAAATAATAAAATGCCCAATGGAAGTGCCAGAAGAAATTGAAAAAAGGATA
AAGGATACAGCTCTTAGAATTTTTAAAGTTTTGAATGCAAGGGATTATGCGAGAATAGATATGATTTTAGGTAAAGACAA
TGTGCCTTATTTCCTAGAGCTCAACACTTATGCTGGACTCACTACCGCAGTAGAAAGAGGGGAAAAACATGTACACCACG
GCTATATGGGATATATGGCAAAAGCTGCTGGCATGACTAGGAAAGAGTTTATAGGAAAAATCTTAGAGAGCGCCCTTGAA
AGGTATGGCTTTGAAGATAAAGAATTACTTTTAGCGTGA

Upstream 100 bases:

>100_bases
GACTGACATGGTAGTAAATCACAGTTCAAAAGTCTTTAAATAAACCTATTTCTCTTTTTCTTTTCCTTTTGCAAAATTTT
TGTAGAAGGAGGAGAAAGGG

Downstream 100 bases:

>100_bases
ACTTGATTTTTGTGCGGAGTTTATATACACTTTGAGTAAGGCATGTTTTTGCCTTATTTTCTTTTTTAGTTAGAAAGGAG
GGAATTTTATGAATTTTAAA

Product: D-alanine-D-alanine ligase and related ATP-grasp enzyme

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKVGVFWRRFRNVELQRRLTPDKVYDDAYDEAYHHYEALKEAGFDAVLIEWKDDPIKTYEVIQKEEVDIIFNASSLKEIE
FLELFNIPYVGSGIDLAATDKRMRKDIVAAHGLPTPKYVVAYSANEIPSVDHLRFPLFVKPIMGRGSAGIDEENIVYDKS
RLPKVVSKITEKIGQPALIEEFIEGREVTVGIIGYRNPIVLPLLEIGYNNVKTNTYEHKMFDNEIIKCPMEVPEEIEKRI
KDTALRIFKVLNARDYARIDMILGKDNVPYFLELNTYAGLTTAVERGEKHVHHGYMGYMAKAAGMTRKEFIGKILESALE
RYGFEDKELLLA

Sequences:

>Translated_332_residues
MKVGVFWRRFRNVELQRRLTPDKVYDDAYDEAYHHYEALKEAGFDAVLIEWKDDPIKTYEVIQKEEVDIIFNASSLKEIE
FLELFNIPYVGSGIDLAATDKRMRKDIVAAHGLPTPKYVVAYSANEIPSVDHLRFPLFVKPIMGRGSAGIDEENIVYDKS
RLPKVVSKITEKIGQPALIEEFIEGREVTVGIIGYRNPIVLPLLEIGYNNVKTNTYEHKMFDNEIIKCPMEVPEEIEKRI
KDTALRIFKVLNARDYARIDMILGKDNVPYFLELNTYAGLTTAVERGEKHVHHGYMGYMAKAAGMTRKEFIGKILESALE
RYGFEDKELLLA
>Mature_332_residues
MKVGVFWRRFRNVELQRRLTPDKVYDDAYDEAYHHYEALKEAGFDAVLIEWKDDPIKTYEVIQKEEVDIIFNASSLKEIE
FLELFNIPYVGSGIDLAATDKRMRKDIVAAHGLPTPKYVVAYSANEIPSVDHLRFPLFVKPIMGRGSAGIDEENIVYDKS
RLPKVVSKITEKIGQPALIEEFIEGREVTVGIIGYRNPIVLPLLEIGYNNVKTNTYEHKMFDNEIIKCPMEVPEEIEKRI
KDTALRIFKVLNARDYARIDMILGKDNVPYFLELNTYAGLTTAVERGEKHVHHGYMGYMAKAAGMTRKEFIGKILESALE
RYGFEDKELLLA

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=298, Percent_Identity=26.8456375838926, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786579, Length=260, Percent_Identity=25.7692307692308, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 38144; Mature: 38144

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGVFWRRFRNVELQRRLTPDKVYDDAYDEAYHHYEALKEAGFDAVLIEWKDDPIKTYE
CCCCHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
VIQKEEVDIIFNASSLKEIEFLELFNIPYVGSGIDLAATDKRMRKDIVAAHGLPTPKYVV
HHHHCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
AYSANEIPSVDHLRFPLFVKPIMGRGSAGIDEENIVYDKSRLPKVVSKITEKIGQPALIE
EECCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHH
EFIEGREVTVGIIGYRNPIVLPLLEIGYNNVKTNTYEHKMFDNEIIKCPMEVPEEIEKRI
HHHCCCEEEEEEEECCCCCEEHHHHCCCCCCCCCCCHHHHHCCHHHCCCCCCHHHHHHHH
KDTALRIFKVLNARDYARIDMILGKDNVPYFLELNTYAGLTTAVERGEKHVHHGYMGYMA
HHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHCHHHHHHHHHHHHH
KAAGMTRKEFIGKILESALERYGFEDKELLLA
HHCCCHHHHHHHHHHHHHHHHCCCCCHHEECC
>Mature Secondary Structure
MKVGVFWRRFRNVELQRRLTPDKVYDDAYDEAYHHYEALKEAGFDAVLIEWKDDPIKTYE
CCCCHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
VIQKEEVDIIFNASSLKEIEFLELFNIPYVGSGIDLAATDKRMRKDIVAAHGLPTPKYVV
HHHHCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
AYSANEIPSVDHLRFPLFVKPIMGRGSAGIDEENIVYDKSRLPKVVSKITEKIGQPALIE
EECCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHH
EFIEGREVTVGIIGYRNPIVLPLLEIGYNNVKTNTYEHKMFDNEIIKCPMEVPEEIEKRI
HHHCCCEEEEEEEECCCCCEEHHHHCCCCCCCCCCCHHHHHCCHHHCCCCCCHHHHHHHH
KDTALRIFKVLNARDYARIDMILGKDNVPYFLELNTYAGLTTAVERGEKHVHHGYMGYMA
HHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHCHHHHHHHHHHHHH
KAAGMTRKEFIGKILESALERYGFEDKELLLA
HHCCCHHHHHHHHHHHHHHHHCCCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA