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The map label for this gene is LeuB

Identifier: 20806561

GI number: 20806561

Start: 22099

End: 23166

Strand: Direct

Name: LeuB

Synonym: TTE0019

Alternate gene names: 20806561

Gene position: 22099-23166 (Clockwise)

Preceding gene: 20806560

Following gene: 20806562

Centisome position: 0.82

GC content: 43.54

Gene sequence:

>1068_bases
ATGTACAGGATAGCAGTTTTGCCAGGAGATGGGATAGGTCCAGAAGTGATAGAAGAAGGTTTAAAGGTTTTAAAAGCTGT
GGAAGAAAAGTACGGGCTCAGATTTGATATAAAGAAATATCCTTTTGGAGGAGAGGCTATTGACAAGTATGGGGAGCCTT
ATCCTGAGGAGACGCGAAATGGCTGCCTTTCAAGTGATGCAGTTCTTCTGGGGGCTGTAGGGGGACCTAAGTGGGACGGT
TTAGAGGGAGATAAAAGACCTGAAGCAGGTCTTCTGGCCTTAAGGAAAAGTTTGGGGGTGTACGCGAATTTAAGGCCGGC
GGTTTTATACCCTTCTTTAAAAGATGCTTCTCCTTTAAAGAATGAACTTTTGGAAAAAGGGCTGGATATACTTGTCGTAA
GAGAGCTCACAGGCGGAATTTACTTTGGACCAAGAGGAAAAGAGGCTATAGGATATGGATTTAGGGCTTATGATACAGAA
GTTTATGAGACGCAGGAGATAGAAAGAATTGCAGTTATCGCTTTTGAGGCGGCCTTAAGGAGGAGAAAAAAGATTACATC
TGTCGACAAGGCAAACGTGTTGGAATCTTCAAGGCTTTGGAGAAAGACTGTAGAAGAAGTTGCTAAGAGGTATCCTGAGG
TGGAGCTAAACCACATGTACGTAGATAACTGTGCGATGCAGCTTGTGAAAAACCCTTTCCAGTTTGATGTGATTCTCACC
AATAATATGTTTGGAGATATTTTGAGCGATGAAGCAGCGCAAATCGTGGGCTCTATTGGAATACTCCCTTCTGCCAGCTT
AAGAGGAGATAGGGTTGGACTTTATGAGCCAATACACGGGTCTGCTCCGGATATTGCCGGAAAGAAAATAGCTAATCCTC
TTGCCACAATTTTATCTGTTGCGATGATGCTTCAGTATTCATTTGGCGAGGAGGAGGCTGCACAGGATATAAGAAAAGCG
GTGGAAAGAGCTTTGGAGGAAGGCTTTAGAACAGCAGATTTGGGCAAAGGAATTGTGCTTTCTACAGAAGAGATGGGAGA
TAAAGTTGTTGAGTATATAAAGGGGTGA

Upstream 100 bases:

>100_bases
CAAAAGGGACAGAATTTAAAGCACAGCCATTTCCGGAATTTATAAAAGAGATAATAAAATATGGAGGACTTATAAATTAC
GTGAGAGAGAAGGTGCGTTA

Downstream 100 bases:

>100_bases
TATTTTGAGAAGCGATGCTGTAAAAAAAGGGGTGGAGAGGGCACCTCATAGGTCCTTGCTTTATGCCTTAGGGCTTACTG
ATGAAGAGCTAAAAAGGCCT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MYRIAVLPGDGIGPEVIEEGLKVLKAVEEKYGLRFDIKKYPFGGEAIDKYGEPYPEETRNGCLSSDAVLLGAVGGPKWDG
LEGDKRPEAGLLALRKSLGVYANLRPAVLYPSLKDASPLKNELLEKGLDILVVRELTGGIYFGPRGKEAIGYGFRAYDTE
VYETQEIERIAVIAFEAALRRRKKITSVDKANVLESSRLWRKTVEEVAKRYPEVELNHMYVDNCAMQLVKNPFQFDVILT
NNMFGDILSDEAAQIVGSIGILPSASLRGDRVGLYEPIHGSAPDIAGKKIANPLATILSVAMMLQYSFGEEEAAQDIRKA
VERALEEGFRTADLGKGIVLSTEEMGDKVVEYIKG

Sequences:

>Translated_355_residues
MYRIAVLPGDGIGPEVIEEGLKVLKAVEEKYGLRFDIKKYPFGGEAIDKYGEPYPEETRNGCLSSDAVLLGAVGGPKWDG
LEGDKRPEAGLLALRKSLGVYANLRPAVLYPSLKDASPLKNELLEKGLDILVVRELTGGIYFGPRGKEAIGYGFRAYDTE
VYETQEIERIAVIAFEAALRRRKKITSVDKANVLESSRLWRKTVEEVAKRYPEVELNHMYVDNCAMQLVKNPFQFDVILT
NNMFGDILSDEAAQIVGSIGILPSASLRGDRVGLYEPIHGSAPDIAGKKIANPLATILSVAMMLQYSFGEEEAAQDIRKA
VERALEEGFRTADLGKGIVLSTEEMGDKVVEYIKG
>Mature_355_residues
MYRIAVLPGDGIGPEVIEEGLKVLKAVEEKYGLRFDIKKYPFGGEAIDKYGEPYPEETRNGCLSSDAVLLGAVGGPKWDG
LEGDKRPEAGLLALRKSLGVYANLRPAVLYPSLKDASPLKNELLEKGLDILVVRELTGGIYFGPRGKEAIGYGFRAYDTE
VYETQEIERIAVIAFEAALRRRKKITSVDKANVLESSRLWRKTVEEVAKRYPEVELNHMYVDNCAMQLVKNPFQFDVILT
NNMFGDILSDEAAQIVGSIGILPSASLRGDRVGLYEPIHGSAPDIAGKKIANPLATILSVAMMLQYSFGEEEAAQDIRKA
VERALEEGFRTADLGKGIVLSTEEMGDKVVEYIKG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=358, Percent_Identity=33.2402234636872, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI28178816, Length=336, Percent_Identity=27.9761904761905, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4758582, Length=359, Percent_Identity=30.3621169916435, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI28178821, Length=336, Percent_Identity=27.9761904761905, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI28178838, Length=330, Percent_Identity=30, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI28178819, Length=170, Percent_Identity=34.7058823529412, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=58.3098591549296, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1788101, Length=361, Percent_Identity=37.3961218836565, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787381, Length=412, Percent_Identity=27.4271844660194, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=33.0409356725146, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=358, Percent_Identity=31.0055865921788, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17550882, Length=363, Percent_Identity=31.404958677686, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=340, Percent_Identity=30.8823529411765, Blast_Score=127, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=50.6887052341598, Blast_Score=311, Evalue=7e-86,
Organism=Saccharomyces cerevisiae, GI6322097, Length=373, Percent_Identity=38.8739946380697, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324291, Length=342, Percent_Identity=31.5789473684211, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6324709, Length=347, Percent_Identity=29.3948126801153, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24643268, Length=346, Percent_Identity=32.6589595375723, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24643270, Length=346, Percent_Identity=32.6589595375723, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24661184, Length=366, Percent_Identity=30.327868852459, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161078635, Length=338, Percent_Identity=29.585798816568, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078637, Length=338, Percent_Identity=29.585798816568, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078633, Length=338, Percent_Identity=29.585798816568, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24650122, Length=338, Percent_Identity=29.585798816568, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161078639, Length=334, Percent_Identity=29.940119760479, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281362242, Length=339, Percent_Identity=29.4985250737463, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648872, Length=339, Percent_Identity=29.4985250737463, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20130355, Length=213, Percent_Identity=27.2300469483568, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_THETN (Q8RDK0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_621732.1
- ProteinModelPortal:   Q8RDK0
- SMR:   Q8RDK0
- GeneID:   996504
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0019
- NMPDR:   fig|273068.3.peg.31
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   TTEN273068:TTE0019-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39064; Mature: 39064

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 95-95 BINDING 105-105 BINDING 134-134 BINDING 222-222

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRIAVLPGDGIGPEVIEEGLKVLKAVEEKYGLRFDIKKYPFGGEAIDKYGEPYPEETRN
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHCCCCCCHHHHC
GCLSSDAVLLGAVGGPKWDGLEGDKRPEAGLLALRKSLGVYANLRPAVLYPSLKDASPLK
CCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHH
NELLEKGLDILVVRELTGGIYFGPRGKEAIGYGFRAYDTEVYETQEIERIAVIAFEAALR
HHHHHCCCCEEEEEECCCCEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RRKKITSVDKANVLESSRLWRKTVEEVAKRYPEVELNHMYVDNCAMQLVKNPFQFDVILT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEHHHHHHHHHCCCCEEEEEEE
NNMFGDILSDEAAQIVGSIGILPSASLRGDRVGLYEPIHGSAPDIAGKKIANPLATILSV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AMMLQYSFGEEEAAQDIRKAVERALEEGFRTADLGKGIVLSTEEMGDKVVEYIKG
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MYRIAVLPGDGIGPEVIEEGLKVLKAVEEKYGLRFDIKKYPFGGEAIDKYGEPYPEETRN
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHCCCCCCHHHHC
GCLSSDAVLLGAVGGPKWDGLEGDKRPEAGLLALRKSLGVYANLRPAVLYPSLKDASPLK
CCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHH
NELLEKGLDILVVRELTGGIYFGPRGKEAIGYGFRAYDTEVYETQEIERIAVIAFEAALR
HHHHHCCCCEEEEEECCCCEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RRKKITSVDKANVLESSRLWRKTVEEVAKRYPEVELNHMYVDNCAMQLVKNPFQFDVILT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEHHHHHHHHHCCCCEEEEEEE
NNMFGDILSDEAAQIVGSIGILPSASLRGDRVGLYEPIHGSAPDIAGKKIANPLATILSV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AMMLQYSFGEEEAAQDIRKAVERALEEGFRTADLGKGIVLSTEEMGDKVVEYIKG
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336