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The map label for this gene is Pgk

Identifier: 20095098

GI number: 20095098

Start: 1664704

End: 1665924

Strand: Reverse

Name: Pgk

Synonym: MK1662

Alternate gene names: 20095098

Gene position: 1665924-1664704 (Counterclockwise)

Preceding gene: 20095099

Following gene: 20095097

Centisome position: 98.29

GC content: 56.35

Gene sequence:

>1221_bases
ATGTACGAAAACATATCTACTATGGACGATTTCGAGTTCGAAAATAAATGGGTCTTGTTACGAATTGATATAAATTCGAC
GGTTATCGATGGTAAAATTGAGGACGATGAACGTATTAAAAGACATCTCGGGACGATCAAGGAGTTAATGGAACACGACG
CCAGGGTGGCCATTCTAGCACACCAAGGAAGACCGGGTGAGGATGACTTCACGACACTGGAACCACATGCGGAAATCATG
TCCGAAGAACTCGACAACTTCGAGTACGTGCCCGACGTGTTCGGACCCACCGCGAAGAAGAAGATTCGGTCGTTGGAGCC
CGGCGAAGTTATCCTGCTGGAGAACGTGCGGTTCTATTCCGAGGAACGTATCAACCGGGACCCGGAGTGGCACGCACGCC
GACATTTGGTGCGGAACCTGGCCCCGCTGTTCGACATTTTCGTGAACGACGCCTTCGCTGCGGCCCATCGCTCGAACGCT
TCACTGGTCGGGTTCACTCGAAGACTTCCGTCTTGCGTCGGCCGTGTGATGGAGCGCGAGATCGAAGTCCTCGAAACCAT
GGTCAGAGACGAGATGGAGGACGGCGTCTTCGTGATCGGAGGGTCCAAGATCGAGGATGCTATCAAGGTCATACGCCGAG
CCATCGAGATGGACAACGTACGTCGCGTGTTACTGGGAGGTCTCGTGGGCAATCTCTTCCTTTGGGCCTCCGGAGTGGAT
CTAGGGAAACCTTCACGGAAATTCCTGGATATGAAGGGTTACACGGGCTACTTGGACGAGGCACGGGAGCTCCTCGAGGA
AGGTGACGACGTGATCCTCGTACCCGAAGACGTGGCTCTCAACCGCGGTGGAGAGCGCGAGGAGGTAGACGTGGACGAAC
TGCCAGCGGACGCTCCCGTCTTCGACATCGGTACCGGAACGATCGAACGGTATCGCAAGGAGGTCGAAAGCGCGGGTATG
GTGGTCGCGAACGGGCCGATGGGCGTGTACGAGGAACCCGGATTCGAGAAGGGAACGTACGAGGTCCTCAATGCTATCGC
CGATTCGGAGGCTTTCTCCGTGATCGGAGGAGGCCACATAATTGCGGCGGCGAAAGCCTGTGGTGCTTATGACAGCATAG
ATCACGTCAGCACGGGTGGCGGAGCGATGCTCCGAATGCTCGCCGGGGAGCGACTACCGGCCATAGATGCCATCCTCACT
TGCCCGTTCTCGGGGTGCTGA

Upstream 100 bases:

>100_bases
ACGGATTTACGGCTCAGCTCGCGAAAGAATTAGGAGTCGATGAATCAGATATGTGGGAACGGCACGCTAAAATACAATAA
AATATTAGGGGCGAACAGTG

Downstream 100 bases:

>100_bases
GGCGTGGTCGTCGTGGTGTACCACCGTCCCTTCAGCCACTTCCGCGTCAACCATGTGGCCCTTATCGCAGGCAACCTCGG
GGCGAAGCTCCTTGTTTATT

Product: 3-phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MYENISTMDDFEFENKWVLLRIDINSTVIDGKIEDDERIKRHLGTIKELMEHDARVAILAHQGRPGEDDFTTLEPHAEIM
SEELDNFEYVPDVFGPTAKKKIRSLEPGEVILLENVRFYSEERINRDPEWHARRHLVRNLAPLFDIFVNDAFAAAHRSNA
SLVGFTRRLPSCVGRVMEREIEVLETMVRDEMEDGVFVIGGSKIEDAIKVIRRAIEMDNVRRVLLGGLVGNLFLWASGVD
LGKPSRKFLDMKGYTGYLDEARELLEEGDDVILVPEDVALNRGGEREEVDVDELPADAPVFDIGTGTIERYRKEVESAGM
VVANGPMGVYEEPGFEKGTYEVLNAIADSEAFSVIGGGHIIAAAKACGAYDSIDHVSTGGGAMLRMLAGERLPAIDAILT
CPFSGC

Sequences:

>Translated_406_residues
MYENISTMDDFEFENKWVLLRIDINSTVIDGKIEDDERIKRHLGTIKELMEHDARVAILAHQGRPGEDDFTTLEPHAEIM
SEELDNFEYVPDVFGPTAKKKIRSLEPGEVILLENVRFYSEERINRDPEWHARRHLVRNLAPLFDIFVNDAFAAAHRSNA
SLVGFTRRLPSCVGRVMEREIEVLETMVRDEMEDGVFVIGGSKIEDAIKVIRRAIEMDNVRRVLLGGLVGNLFLWASGVD
LGKPSRKFLDMKGYTGYLDEARELLEEGDDVILVPEDVALNRGGEREEVDVDELPADAPVFDIGTGTIERYRKEVESAGM
VVANGPMGVYEEPGFEKGTYEVLNAIADSEAFSVIGGGHIIAAAKACGAYDSIDHVSTGGGAMLRMLAGERLPAIDAILT
CPFSGC
>Mature_406_residues
MYENISTMDDFEFENKWVLLRIDINSTVIDGKIEDDERIKRHLGTIKELMEHDARVAILAHQGRPGEDDFTTLEPHAEIM
SEELDNFEYVPDVFGPTAKKKIRSLEPGEVILLENVRFYSEERINRDPEWHARRHLVRNLAPLFDIFVNDAFAAAHRSNA
SLVGFTRRLPSCVGRVMEREIEVLETMVRDEMEDGVFVIGGSKIEDAIKVIRRAIEMDNVRRVLLGGLVGNLFLWASGVD
LGKPSRKFLDMKGYTGYLDEARELLEEGDDVILVPEDVALNRGGEREEVDVDELPADAPVFDIGTGTIERYRKEVESAGM
VVANGPMGVYEEPGFEKGTYEVLNAIADSEAFSVIGGGHIIAAAKACGAYDSIDHVSTGGGAMLRMLAGERLPAIDAILT
CPFSGC

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=421, Percent_Identity=28.5035629453682, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI31543397, Length=414, Percent_Identity=27.7777777777778, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1789294, Length=401, Percent_Identity=32.9177057356608, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17508823, Length=418, Percent_Identity=29.6650717703349, Blast_Score=161, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6319857, Length=408, Percent_Identity=30.3921568627451, Blast_Score=162, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24581203, Length=420, Percent_Identity=29.5238095238095, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17136394, Length=428, Percent_Identity=28.9719626168224, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_METKA (Q8TUU1)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614945.1
- ProteinModelPortal:   Q8TUU1
- SMR:   Q8TUU1
- GeneID:   1478257
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1662
- NMPDR:   fig|190192.1.peg.1658
- HOGENOM:   HBG453500
- OMA:   PGKKDFT
- BioCyc:   MKAN190192:MK1662-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 45133; Mature: 45133

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 38-38 BINDING 117-117 BINDING 157-157 BINDING 331-331

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYENISTMDDFEFENKWVLLRIDINSTVIDGKIEDDERIKRHLGTIKELMEHDARVAILA
CCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
HQGRPGEDDFTTLEPHAEIMSEELDNFEYVPDVFGPTAKKKIRSLEPGEVILLENVRFYS
ECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCCHHHHHHHCCCCCCEEEECCCHHHH
EERINRDPEWHARRHLVRNLAPLFDIFVNDAFAAAHRSNASLVGFTRRLPSCVGRVMERE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IEVLETMVRDEMEDGVFVIGGSKIEDAIKVIRRAIEMDNVRRVLLGGLVGNLFLWASGVD
HHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LGKPSRKFLDMKGYTGYLDEARELLEEGDDVILVPEDVALNRGGEREEVDVDELPADAPV
CCCCCHHHEECCCCCCHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCHHHCCCCCCE
FDIGTGTIERYRKEVESAGMVVANGPMGVYEEPGFEKGTYEVLNAIADSEAFSVIGGGHI
EECCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCHHEEECCCHH
IAAAKACGAYDSIDHVSTGGGAMLRMLAGERLPAIDAILTCPFSGC
EEHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MYENISTMDDFEFENKWVLLRIDINSTVIDGKIEDDERIKRHLGTIKELMEHDARVAILA
CCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
HQGRPGEDDFTTLEPHAEIMSEELDNFEYVPDVFGPTAKKKIRSLEPGEVILLENVRFYS
ECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCCHHHHHHHCCCCCCEEEECCCHHHH
EERINRDPEWHARRHLVRNLAPLFDIFVNDAFAAAHRSNASLVGFTRRLPSCVGRVMERE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IEVLETMVRDEMEDGVFVIGGSKIEDAIKVIRRAIEMDNVRRVLLGGLVGNLFLWASGVD
HHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LGKPSRKFLDMKGYTGYLDEARELLEEGDDVILVPEDVALNRGGEREEVDVDELPADAPV
CCCCCHHHEECCCCCCHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCHHHCCCCCCE
FDIGTGTIERYRKEVESAGMVVANGPMGVYEEPGFEKGTYEVLNAIADSEAFSVIGGGHI
EECCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCHHEEECCCHH
IAAAKACGAYDSIDHVSTGGGAMLRMLAGERLPAIDAILTCPFSGC
EEHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014