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The map label for this gene is IlvE [H]

Identifier: 20095063

GI number: 20095063

Start: 1628934

End: 1629821

Strand: Reverse

Name: IlvE [H]

Synonym: MK1627

Alternate gene names: 20095063

Gene position: 1629821-1628934 (Counterclockwise)

Preceding gene: 20095066

Following gene: 20095062

Centisome position: 96.16

GC content: 61.26

Gene sequence:

>888_bases
TTGTCTGAGCGCGAGCAGCTCATCTACCTGAACGGCGAACTGGTCCCCCGGGAAGAGGCTAAAATCTCCGTCTACGACCA
CGGGTTCCTGTACGGCGACGGCGTCTTCGAGGGGATTCGGGCCTACGATGGCCGGATATTCAAGTTGGACGAGCACGTCG
ACAGACTGTACGACTCCGCAAAGGCCATCATGCTCGAGATACCGATGACCAAGGAGAAGATGAAGGAGGCCATCATCGAG
ACCGTACGGGCCAACGAGCTCCGGGACGCGTACATCCGGGTGGTGGTCTCGCGCGGCGAAGGTGACCTCGGGTTGGACCC
GGAGAAGTGTCCGGAACCCAACGTGGTGATCATCGCGGAGCCGATGGAGCCGTTGTACGGGGACCTGTACGAGAAAGGGA
TCGAGGTGATCACGGCCTCCGTGCGTCGTATTCCACCGGACGCGCTCGATCCGAAGATCAAGTCGTGCAACTACCTGAAC
AACATCCTGGCGAAGATCCAAGCCAACCTGGCGGGCGCCGACGAGGCCATCATGTTGGACCACGAGGGCTACGTCTGCGA
GGGTACCGGTGACAACGTGTTCGTGGTGGAAGACGGGACCGTGTACACTCCACCGGAGGACACTATTCTCCGGGGGATCA
CCAGGGCGACCGTGATGGAGATCTGCGAGGAGCTCGGTATACCCGTGGAAGAGAAGCGGATAACACTGGGAGAACTGTAC
GCGGCGGACGAGGTGTTCCTAACGGGCACGGCGGCGGAGGTGGCCCCCGTTAGGAAGGTCGACGGGCGGAAGATCGGCGA
GGAGTGCCCAGGACCCATCACGAGGCGTATCATGGAGGCGTTCCGGGAGCTCACGAAGAAGGAAGGTACGCCGGTGTACG
AGGAGTGA

Upstream 100 bases:

>100_bases
CAGGATCACGTACTGGAGCATCTCCCCTCCGAGCCCCACGCGATCCCCGGAGGGAGAGTGTTTTAACGTTACCCGTCGGG
CAGCGGCTGGGGGTTCCGAG

Downstream 100 bases:

>100_bases
CGGGGTCTCTCGTTGTCCCTCCTCAAGCTCGTTAAAGGCAGTCTCACGGTCCTCGTCGGCTCGTTGTTCCTTCGATTAGG
AGGGTACGTGTACCGGCTCC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSEREQLIYLNGELVPREEAKISVYDHGFLYGDGVFEGIRAYDGRIFKLDEHVDRLYDSAKAIMLEIPMTKEKMKEAIIE
TVRANELRDAYIRVVVSRGEGDLGLDPEKCPEPNVVIIAEPMEPLYGDLYEKGIEVITASVRRIPPDALDPKIKSCNYLN
NILAKIQANLAGADEAIMLDHEGYVCEGTGDNVFVVEDGTVYTPPEDTILRGITRATVMEICEELGIPVEEKRITLGELY
AADEVFLTGTAAEVAPVRKVDGRKIGEECPGPITRRIMEAFRELTKKEGTPVYEE

Sequences:

>Translated_295_residues
MSEREQLIYLNGELVPREEAKISVYDHGFLYGDGVFEGIRAYDGRIFKLDEHVDRLYDSAKAIMLEIPMTKEKMKEAIIE
TVRANELRDAYIRVVVSRGEGDLGLDPEKCPEPNVVIIAEPMEPLYGDLYEKGIEVITASVRRIPPDALDPKIKSCNYLN
NILAKIQANLAGADEAIMLDHEGYVCEGTGDNVFVVEDGTVYTPPEDTILRGITRATVMEICEELGIPVEEKRITLGELY
AADEVFLTGTAAEVAPVRKVDGRKIGEECPGPITRRIMEAFRELTKKEGTPVYEE
>Mature_294_residues
SEREQLIYLNGELVPREEAKISVYDHGFLYGDGVFEGIRAYDGRIFKLDEHVDRLYDSAKAIMLEIPMTKEKMKEAIIET
VRANELRDAYIRVVVSRGEGDLGLDPEKCPEPNVVIIAEPMEPLYGDLYEKGIEVITASVRRIPPDALDPKIKSCNYLNN
ILAKIQANLAGADEAIMLDHEGYVCEGTGDNVFVVEDGTVYTPPEDTILRGITRATVMEICEELGIPVEEKRITLGELYA
ADEVFLTGTAAEVAPVRKVDGRKIGEECPGPITRRIMEAFRELTKKEGTPVYEE

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=294, Percent_Identity=39.4557823129252, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1787338, Length=257, Percent_Identity=28.0155642023346, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 32956; Mature: 32825

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEREQLIYLNGELVPREEAKISVYDHGFLYGDGVFEGIRAYDGRIFKLDEHVDRLYDSA
CCCCCEEEEECCEECCCCCCEEEEEECCEEECCHHHHHHHHHCCEEEEHHHHHHHHHHCC
KAIMLEIPMTKEKMKEAIIETVRANELRDAYIRVVVSRGEGDLGLDPEKCPEPNVVIIAE
CEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCEEEEEC
PMEPLYGDLYEKGIEVITASVRRIPPDALDPKIKSCNYLNNILAKIQANLAGADEAIMLD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
HEGYVCEGTGDNVFVVEDGTVYTPPEDTILRGITRATVMEICEELGIPVEEKRITLGELY
CCCEEEECCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEHHHHH
AADEVFLTGTAAEVAPVRKVDGRKIGEECPGPITRRIMEAFRELTKKEGTPVYEE
CCCEEEEECCHHHHCCHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEREQLIYLNGELVPREEAKISVYDHGFLYGDGVFEGIRAYDGRIFKLDEHVDRLYDSA
CCCCEEEEECCEECCCCCCEEEEEECCEEECCHHHHHHHHHCCEEEEHHHHHHHHHHCC
KAIMLEIPMTKEKMKEAIIETVRANELRDAYIRVVVSRGEGDLGLDPEKCPEPNVVIIAE
CEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCEEEEEC
PMEPLYGDLYEKGIEVITASVRRIPPDALDPKIKSCNYLNNILAKIQANLAGADEAIMLD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
HEGYVCEGTGDNVFVVEDGTVYTPPEDTILRGITRATVMEICEELGIPVEEKRITLGELY
CCCEEEECCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEHHHHH
AADEVFLTGTAAEVAPVRKVDGRKIGEECPGPITRRIMEAFRELTKKEGTPVYEE
CCCEEEEECCHHHHCCHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]