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The map label for this gene is TopA [H]

Identifier: 20095040

GI number: 20095040

Start: 1612436

End: 1614199

Strand: Direct

Name: TopA [H]

Synonym: MK1604

Alternate gene names: 20095040

Gene position: 1612436-1614199 (Clockwise)

Preceding gene: 20095039

Following gene: 20095041

Centisome position: 95.13

GC content: 61.68

Gene sequence:

>1764_bases
ATGGCCTCGTCGTCGAAGGAGACGATGGTAATCATAGCCGAAAAGCCCAGTCTCGCGGGAACGATCGCGGGGTTCCTTCG
GGGGAGCTGGGACGGTAGGAAGCTGCTGGGGTTCGGTAGATACCGAGGTAGACGGTTCGCCATCACCAGCCTGTCGGGCC
ACGTCCTGGAGTGGTGGCCTAAAGACGATCCGGGGTTCAGGCACCCTGATTACTTCCCCGACCCGGGCGACTTCGAGTTG
AGGCCTATCGACGGTAAAGAAAGGTTCCTGCGGGCCGTGGAACGTGTCGTGAAGCGCTATGCCGGCTGTCGAGCCGACAG
GGTCATCGTAGCGACCGACAACGACGCGGAGGGAGAGCTGATCGGTTGGGAAGTACTTGTATGGTTGAAACGACAGGGTG
GCCTCGACGATCCCGAGTGCGCTCGGCGGATGCGGTTCTCGGCGTACACCCGCGAGGACGTCCTCAGGGCGCTCGAGGGG
GCACTCCGTGGTGAGAGGATCGACCCGAGCCTGGCTTACTCAGCGCTCGCACGCACGATCGCGGACTGGCTGTACGGGAT
ACCGCTCACCCGTCGGCTGTCGCTCTGCAACGACGACATAGTCTCCGTCGGTCGAGTACAGACGCCCACACTCAAACTGG
TCGTGGAGCGGGAGCGGGAACGCAGGAAGGCTCAGAAGAAGCGCAGATATTACTGGATACTCCAAGCCGAGACCCCGATC
GGGGAACTTAGAACTGAAGAGAAGTTCAAAGATGGACGTCGGGCGAGGGAGCTCGCCTCCGAAATCGAATCGATAAGGGT
CGTGGAAGTGCGGCGGGAGCGCCGGTCCGTAAGACCACCTACTCCGTTCAACCTGACTACCCTTCAACGGGCCGCCGGTA
AGATCCTCCGGATATCGCCGAAGCGCACCCTCGATTTGGCTCAGCGCCTCTACGAGGAAGGGATGATCACGTACCCGAGA
ACCGCGACTAACCGGTACCCATCCACGTTCGACCACGAAGAACTTCTCCGCAAGCTGAGGAACGCTCACCCCGATGCCCT
CCGGGATTTCCAGCGCACTGGTCGAAGGTCAGAGCCCGTCTCCGGTAAGGAGTACGACGGCGCCCATCCCCCGATCACCC
CCACCGGTCGGCGGAAGTACATCCGCGGGAAACTGGCCTGGCGCCTGTACGATCTGATCGTCCGTCGATATCTGGCGACA
CTGTCCGAGGACGCCCTGGTAGTGAAGTGGCGGATCGTAGCCGAGCATCCAGGGACCGGGACGAGGTTCGTGATGGAAGG
CACGGAAGTCGAGCGGGACGGGTGGTACTCGGTGTACCCGTGGGAGAAGCCCCGGGAAAGTACCATGCCCGACGTGTCCG
AGGGAGACGAGCTACCGGCGAATGTAAACGCTTCCCGCCGTAGGAAACCCCTCCCTCGTCGGTACTCGCAGTCGCGGTTG
GTGGCGAAAATGAAGAAGCTTGGATTGGGTACCGAGTCCACACGGGCGGAGATCGTGAAGAAACTCTTTGACAGGGGTTA
CGTCAAGAGGGCAGGGTCGGGCGTGGCCCCTACGAAGAGAGGGGAGCGTCTGGTAGAGCTGCTCGAGGATCGGGTTCCGG
AGCTCGTGAGCGTCGAACTCACCAGGCGCATTGAGCGCGAGATGGAGGAGATCTCGGAGTTACCGCCGAAGCGAGCCAGG
GAACGATTGGAACGCGTAGCCCGGGAGATCCGCGAGACCGTGCGCAGGAACTCCAAGAAGTTGAAGTCAGCCAAGGTGGT
CTGA

Upstream 100 bases:

>100_bases
GGGGCACCGAGGATATCGTTTCCTGCAACCTCCGTGTGGAACCGTCGGAGGGGAACGGAGGCGAGAATAACCAGGTTCAT
CCCTTTCAGGGGCTCGTGCT

Downstream 100 bases:

>100_bases
CTCCTACTCCGACCTCGCCCTTGTCACCACCGAAACAACACTCACATGAACCGACGCTTGACCTTGAGCTCTCACATTCT
GGGCACGTTGTGATAGCGTG

Product: topoisomerase IA

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MASSSKETMVIIAEKPSLAGTIAGFLRGSWDGRKLLGFGRYRGRRFAITSLSGHVLEWWPKDDPGFRHPDYFPDPGDFEL
RPIDGKERFLRAVERVVKRYAGCRADRVIVATDNDAEGELIGWEVLVWLKRQGGLDDPECARRMRFSAYTREDVLRALEG
ALRGERIDPSLAYSALARTIADWLYGIPLTRRLSLCNDDIVSVGRVQTPTLKLVVERERERRKAQKKRRYYWILQAETPI
GELRTEEKFKDGRRARELASEIESIRVVEVRRERRSVRPPTPFNLTTLQRAAGKILRISPKRTLDLAQRLYEEGMITYPR
TATNRYPSTFDHEELLRKLRNAHPDALRDFQRTGRRSEPVSGKEYDGAHPPITPTGRRKYIRGKLAWRLYDLIVRRYLAT
LSEDALVVKWRIVAEHPGTGTRFVMEGTEVERDGWYSVYPWEKPRESTMPDVSEGDELPANVNASRRRKPLPRRYSQSRL
VAKMKKLGLGTESTRAEIVKKLFDRGYVKRAGSGVAPTKRGERLVELLEDRVPELVSVELTRRIEREMEEISELPPKRAR
ERLERVAREIRETVRRNSKKLKSAKVV

Sequences:

>Translated_587_residues
MASSSKETMVIIAEKPSLAGTIAGFLRGSWDGRKLLGFGRYRGRRFAITSLSGHVLEWWPKDDPGFRHPDYFPDPGDFEL
RPIDGKERFLRAVERVVKRYAGCRADRVIVATDNDAEGELIGWEVLVWLKRQGGLDDPECARRMRFSAYTREDVLRALEG
ALRGERIDPSLAYSALARTIADWLYGIPLTRRLSLCNDDIVSVGRVQTPTLKLVVERERERRKAQKKRRYYWILQAETPI
GELRTEEKFKDGRRARELASEIESIRVVEVRRERRSVRPPTPFNLTTLQRAAGKILRISPKRTLDLAQRLYEEGMITYPR
TATNRYPSTFDHEELLRKLRNAHPDALRDFQRTGRRSEPVSGKEYDGAHPPITPTGRRKYIRGKLAWRLYDLIVRRYLAT
LSEDALVVKWRIVAEHPGTGTRFVMEGTEVERDGWYSVYPWEKPRESTMPDVSEGDELPANVNASRRRKPLPRRYSQSRL
VAKMKKLGLGTESTRAEIVKKLFDRGYVKRAGSGVAPTKRGERLVELLEDRVPELVSVELTRRIEREMEEISELPPKRAR
ERLERVAREIRETVRRNSKKLKSAKVV
>Mature_586_residues
ASSSKETMVIIAEKPSLAGTIAGFLRGSWDGRKLLGFGRYRGRRFAITSLSGHVLEWWPKDDPGFRHPDYFPDPGDFELR
PIDGKERFLRAVERVVKRYAGCRADRVIVATDNDAEGELIGWEVLVWLKRQGGLDDPECARRMRFSAYTREDVLRALEGA
LRGERIDPSLAYSALARTIADWLYGIPLTRRLSLCNDDIVSVGRVQTPTLKLVVERERERRKAQKKRRYYWILQAETPIG
ELRTEEKFKDGRRARELASEIESIRVVEVRRERRSVRPPTPFNLTTLQRAAGKILRISPKRTLDLAQRLYEEGMITYPRT
ATNRYPSTFDHEELLRKLRNAHPDALRDFQRTGRRSEPVSGKEYDGAHPPITPTGRRKYIRGKLAWRLYDLIVRRYLATL
SEDALVVKWRIVAEHPGTGTRFVMEGTEVERDGWYSVYPWEKPRESTMPDVSEGDELPANVNASRRRKPLPRRYSQSRLV
AKMKKLGLGTESTRAEIVKKLFDRGYVKRAGSGVAPTKRGERLVELLEDRVPELVSVELTRRIEREMEEISELPPKRARE
RLERVAREIRETVRRNSKKLKSAKVV

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=603, Percent_Identity=30.016583747927, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI10835218, Length=496, Percent_Identity=28.4274193548387, Blast_Score=139, Evalue=9e-33,
Organism=Escherichia coli, GI1788061, Length=600, Percent_Identity=30.1666666666667, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1787529, Length=583, Percent_Identity=26.5866209262436, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32563869, Length=600, Percent_Identity=26.5, Blast_Score=155, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17555378, Length=588, Percent_Identity=24.4897959183673, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323263, Length=675, Percent_Identity=25.4814814814815, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24640096, Length=600, Percent_Identity=27.1666666666667, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24585251, Length=543, Percent_Identity=27.4401473296501, Blast_Score=145, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005739
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 67912; Mature: 67781

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSSKETMVIIAEKPSLAGTIAGFLRGSWDGRKLLGFGRYRGRRFAITSLSGHVLEWWP
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCCEEEECC
KDDPGFRHPDYFPDPGDFELRPIDGKERFLRAVERVVKRYAGCRADRVIVATDNDAEGEL
CCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCE
IGWEVLVWLKRQGGLDDPECARRMRFSAYTREDVLRALEGALRGERIDPSLAYSALARTI
EHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ADWLYGIPLTRRLSLCNDDIVSVGRVQTPTLKLVVERERERRKAQKKRRYYWILQAETPI
HHHHHCCCHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCH
GELRTEEKFKDGRRARELASEIESIRVVEVRRERRSVRPPTPFNLTTLQRAAGKILRISP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECC
KRTLDLAQRLYEEGMITYPRTATNRYPSTFDHEELLRKLRNAHPDALRDFQRTGRRSEPV
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
SGKEYDGAHPPITPTGRRKYIRGKLAWRLYDLIVRRYLATLSEDALVVKWRIVAEHPGTG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCC
TRFVMEGTEVERDGWYSVYPWEKPRESTMPDVSEGDELPANVNASRRRKPLPRRYSQSRL
CEEEEECCEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHH
VAKMKKLGLGTESTRAEIVKKLFDRGYVKRAGSGVAPTKRGERLVELLEDRVPELVSVEL
HHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
TRRIEREMEEISELPPKRARERLERVAREIRETVRRNSKKLKSAKVV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASSSKETMVIIAEKPSLAGTIAGFLRGSWDGRKLLGFGRYRGRRFAITSLSGHVLEWWP
CCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCCEEEECC
KDDPGFRHPDYFPDPGDFELRPIDGKERFLRAVERVVKRYAGCRADRVIVATDNDAEGEL
CCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCE
IGWEVLVWLKRQGGLDDPECARRMRFSAYTREDVLRALEGALRGERIDPSLAYSALARTI
EHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ADWLYGIPLTRRLSLCNDDIVSVGRVQTPTLKLVVERERERRKAQKKRRYYWILQAETPI
HHHHHCCCHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCH
GELRTEEKFKDGRRARELASEIESIRVVEVRRERRSVRPPTPFNLTTLQRAAGKILRISP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECC
KRTLDLAQRLYEEGMITYPRTATNRYPSTFDHEELLRKLRNAHPDALRDFQRTGRRSEPV
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
SGKEYDGAHPPITPTGRRKYIRGKLAWRLYDLIVRRYLATLSEDALVVKWRIVAEHPGTG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCC
TRFVMEGTEVERDGWYSVYPWEKPRESTMPDVSEGDELPANVNASRRRKPLPRRYSQSRL
CEEEEECCEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHH
VAKMKKLGLGTESTRAEIVKKLFDRGYVKRAGSGVAPTKRGERLVELLEDRVPELVSVEL
HHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
TRRIEREMEEISELPPKRARERLERVAREIRETVRRNSKKLKSAKVV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]