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The map label for this gene is HisS

Identifier: 20094974

GI number: 20094974

Start: 1555348

End: 1556613

Strand: Reverse

Name: HisS

Synonym: MK1538

Alternate gene names: 20094974

Gene position: 1556613-1555348 (Counterclockwise)

Preceding gene: 20094975

Following gene: 20094973

Centisome position: 91.84

GC content: 62.01

Gene sequence:

>1266_bases
ATGGGGGTTACGCTGATGAAGGTGGAGCGACCCAAGGGCACCCGCGACTTCACCCCGGAGGAGATGAGGGCACGCAGATG
GCTGGAGCGGTACCTGTTGGACGTGTTCCGCTCTTACGGGTACGAGGAGGTCCTCACTCCCACCTTCGAGCATGCGAAGC
TGTTCGAGGAGAAGTCCGGTGAGGAGATACTGGAAGAAATGTACGTGTTCAAGGACAAGAAGGGGAGGAAGCTTGCGCTC
AGGCCGGAGATGACGGCGCCTGTCGTCCGGTTTTACAACGCGGAGCTGAAGACGCGACCTCACCCTCTCAAGCTGGCGTA
CATCGTGAACTGCTTCCGGTACGAGCAGCCGCAGCGGGGACGTTGGCGTGAGTTCTGGCAGGCCGGCGTGGAGGTGTTCG
GCTCGGACCGCCCGGAGGCGGACGTCGAGGTGATCGAGCTGACCTACCGGATCTTCGACGGCATCTTGCCTTCCGGTGCC
TTCGAAGTCCGGGTGGGACATCTGGGAATACTCCGGGGATTGCTCGAAGAGTACGGAATAGGTGAAGATGTACAGAACAA
GGTAGCACACCTCGTCGACAAGGGCGAACTGGATGAAGTGAAGACGTTGCTACCGGCCGAGCCGGCCGAGAAGGCCCTAG
CGGTGGTGACGGCGCGCTCGGAGTCCGAGGTGGAGGAGGCCGTGAGTGGGAAAGAACGCGCCGAGCGGGCCCTGGAGAAC
CTCATGGAAATCTCGGACGCGCTCCGGGAAGCGGGGGTCGACCACGAACTCGATTTCTCCGTCGCGAGAGGGTTGGATTA
CTACACCGGGATGGTGTTCGAAATACACGTGCCGGAGCTCGGTGGAGGATCCCAGTGCGCCGGTGGCGGCCGCTACGACG
ACCTGGTGAAGGAGCTCGGAGGACCCGACGTCCCGGCCGTCGGCATGGCCATAGGTTTCGACAGGCTCCTCCTCGCGGCC
GAGCTGTACGACCGGATCCCGGACGGCGTGGAGGAAACCCGTGCCTTGCTGATACCGCTCGTGAGGTCGGGTAAGATCTG
GGAGATAGCCGCGAAGCTGCGCAAGCTCGGGTGGGTGGTGAACGTAGAGGTTTCAGGAAAGCACATACGCAAGGCGCTCT
CACTGGCGGACTCGCTCGAGTACGACTACGCCGTGATCGTAGGGGAGCGGGAGCTGAAGGAGGGATACGTGTCGGTGAAG
AACCTGAAAACCGGGGTTCAGGAGGAAGTCCCGCTGGATCAACTCGAGAGGGCGGCTGAAGTATGA

Upstream 100 bases:

>100_bases
TCGGTGACCAGGGGTTCGACATGCCCGAGGCGTGCCGACTGTTCCCGCACCGACATGAGACCTGTGGATTCTTCATAGCA
CTCATGGGACGGACCGGCTG

Downstream 100 bases:

>100_bases
GTCACGTACCACTGGATCCCGAGACCGCGCGGAAGGTCACGTCCTCCCTCAACTACCGGTTCGAGATCGGCGGGGAGCCG
TTGGTGATCGCGATCGCCCA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MGVTLMKVERPKGTRDFTPEEMRARRWLERYLLDVFRSYGYEEVLTPTFEHAKLFEEKSGEEILEEMYVFKDKKGRKLAL
RPEMTAPVVRFYNAELKTRPHPLKLAYIVNCFRYEQPQRGRWREFWQAGVEVFGSDRPEADVEVIELTYRIFDGILPSGA
FEVRVGHLGILRGLLEEYGIGEDVQNKVAHLVDKGELDEVKTLLPAEPAEKALAVVTARSESEVEEAVSGKERAERALEN
LMEISDALREAGVDHELDFSVARGLDYYTGMVFEIHVPELGGGSQCAGGGRYDDLVKELGGPDVPAVGMAIGFDRLLLAA
ELYDRIPDGVEETRALLIPLVRSGKIWEIAAKLRKLGWVVNVEVSGKHIRKALSLADSLEYDYAVIVGERELKEGYVSVK
NLKTGVQEEVPLDQLERAAEV

Sequences:

>Translated_421_residues
MGVTLMKVERPKGTRDFTPEEMRARRWLERYLLDVFRSYGYEEVLTPTFEHAKLFEEKSGEEILEEMYVFKDKKGRKLAL
RPEMTAPVVRFYNAELKTRPHPLKLAYIVNCFRYEQPQRGRWREFWQAGVEVFGSDRPEADVEVIELTYRIFDGILPSGA
FEVRVGHLGILRGLLEEYGIGEDVQNKVAHLVDKGELDEVKTLLPAEPAEKALAVVTARSESEVEEAVSGKERAERALEN
LMEISDALREAGVDHELDFSVARGLDYYTGMVFEIHVPELGGGSQCAGGGRYDDLVKELGGPDVPAVGMAIGFDRLLLAA
ELYDRIPDGVEETRALLIPLVRSGKIWEIAAKLRKLGWVVNVEVSGKHIRKALSLADSLEYDYAVIVGERELKEGYVSVK
NLKTGVQEEVPLDQLERAAEV
>Mature_420_residues
GVTLMKVERPKGTRDFTPEEMRARRWLERYLLDVFRSYGYEEVLTPTFEHAKLFEEKSGEEILEEMYVFKDKKGRKLALR
PEMTAPVVRFYNAELKTRPHPLKLAYIVNCFRYEQPQRGRWREFWQAGVEVFGSDRPEADVEVIELTYRIFDGILPSGAF
EVRVGHLGILRGLLEEYGIGEDVQNKVAHLVDKGELDEVKTLLPAEPAEKALAVVTARSESEVEEAVSGKERAERALENL
MEISDALREAGVDHELDFSVARGLDYYTGMVFEIHVPELGGGSQCAGGGRYDDLVKELGGPDVPAVGMAIGFDRLLLAAE
LYDRIPDGVEETRALLIPLVRSGKIWEIAAKLRKLGWVVNVEVSGKHIRKALSLADSLEYDYAVIVGERELKEGYVSVKN
LKTGVQEEVPLDQLERAAEV

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=437, Percent_Identity=28.604118993135, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI15029520, Length=453, Percent_Identity=27.5938189845475, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1788861, Length=442, Percent_Identity=32.1266968325792, Blast_Score=171, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI71993686, Length=461, Percent_Identity=28.4164859002169, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71993693, Length=461, Percent_Identity=28.4164859002169, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325290, Length=463, Percent_Identity=24.1900647948164, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24643061, Length=464, Percent_Identity=26.7241379310345, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24643059, Length=483, Percent_Identity=25.6728778467909, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_METKA (Q8TV61)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614821.1
- ProteinModelPortal:   Q8TV61
- SMR:   Q8TV61
- GeneID:   1478133
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1538
- NMPDR:   fig|190192.1.peg.1534
- HOGENOM:   HBG616575
- OMA:   YYFANCF
- BioCyc:   MKAN190192:MK1538-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47471; Mature: 47340

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVTLMKVERPKGTRDFTPEEMRARRWLERYLLDVFRSYGYEEVLTPTFEHAKLFEEKSG
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHCCH
EEILEEMYVFKDKKGRKLALRPEMTAPVVRFYNAELKTRPHPLKLAYIVNCFRYEQPQRG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCHHCCCCCCCHHHHHHHHHHCCCCCCC
RWREFWQAGVEVFGSDRPEADVEVIELTYRIFDGILPSGAFEVRVGHLGILRGLLEEYGI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCC
GEDVQNKVAHLVDKGELDEVKTLLPAEPAEKALAVVTARSESEVEEAVSGKERAERALEN
CHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHEEHEECCCHHHHHHHHCCHHHHHHHHHH
LMEISDALREAGVDHELDFSVARGLDYYTGMVFEIHVPELGGGSQCAGGGRYDDLVKELG
HHHHHHHHHHCCCCCCCCHHHHHCCHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCC
GPDVPAVGMAIGFDRLLLAAELYDRIPDGVEETRALLIPLVRSGKIWEIAAKLRKLGWVV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEE
NVEVSGKHIRKALSLADSLEYDYAVIVGERELKEGYVSVKNLKTGVQEEVPLDQLERAAE
EEEECHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHC
V
C
>Mature Secondary Structure 
GVTLMKVERPKGTRDFTPEEMRARRWLERYLLDVFRSYGYEEVLTPTFEHAKLFEEKSG
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHCCH
EEILEEMYVFKDKKGRKLALRPEMTAPVVRFYNAELKTRPHPLKLAYIVNCFRYEQPQRG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCHHCCCCCCCHHHHHHHHHHCCCCCCC
RWREFWQAGVEVFGSDRPEADVEVIELTYRIFDGILPSGAFEVRVGHLGILRGLLEEYGI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCC
GEDVQNKVAHLVDKGELDEVKTLLPAEPAEKALAVVTARSESEVEEAVSGKERAERALEN
CHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHEEHEECCCHHHHHHHHCCHHHHHHHHHH
LMEISDALREAGVDHELDFSVARGLDYYTGMVFEIHVPELGGGSQCAGGGRYDDLVKELG
HHHHHHHHHHCCCCCCCCHHHHHCCHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCC
GPDVPAVGMAIGFDRLLLAAELYDRIPDGVEETRALLIPLVRSGKIWEIAAKLRKLGWVV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEE
NVEVSGKHIRKALSLADSLEYDYAVIVGERELKEGYVSVKNLKTGVQEEVPLDQLERAAE
EEEECHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014