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The map label for this gene is hisI

Identifier: 20094973

GI number: 20094973

Start: 1554926

End: 1555351

Strand: Reverse

Name: hisI

Synonym: MK1537

Alternate gene names: 20094973

Gene position: 1555351-1554926 (Counterclockwise)

Preceding gene: 20094974

Following gene: 20094972

Centisome position: 91.76

GC content: 61.27

Gene sequence:

>426_bases
ATGAGTCACGTACCACTGGATCCCGAGACCGCGCGGAAGGTCACGTCCTCCCTCAACTACCGGTTCGAGATCGGCGGGGA
GCCGTTGGTGATCGCGATCGCCCAGGACGCCGAGAACGGTGATGTGTTGATGACGGCGTTCGCGAACGAGGAGGCGGTAT
ACCGGACGCTCACGACGGGTTACGCCCACTACTGGAGCACGAGTCGGCGCGAGGTATGGAAGAAAGGGGAGGAGTCGGGT
CACGTGCAACGGGTCGTCGAGGTCCGCGTGGACTGTGACAAAGACGCCGTCCTGTACGTGGTAGAGCAGGAAGGTGGGGC
CTGCCACACCGGTTACAGGTCCTGTTTCTACCGACGCGTTACTCGGGACGGATCGTTCGAGGTCGTGATGGATCGAGTTT
TCAACCCGGACGAGGTGTACCGTTAA

Upstream 100 bases:

>100_bases
GGGAGCGGGAGCTGAAGGAGGGATACGTGTCGGTGAAGAACCTGAAAACCGGGGTTCAGGAGGAAGTCCCGCTGGATCAA
CTCGAGAGGGCGGCTGAAGT

Downstream 100 bases:

>100_bases
TATACTGATACAGGCCACGAGAGGACGAAGTCGGTGAACGCGACACGTGGGGAAGGTGTGGGATCCATGGATGTAGAGAT
CGAGACGGAGTACGTCGTGC

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MSHVPLDPETARKVTSSLNYRFEIGGEPLVIAIAQDAENGDVLMTAFANEEAVYRTLTTGYAHYWSTSRREVWKKGEESG
HVQRVVEVRVDCDKDAVLYVVEQEGGACHTGYRSCFYRRVTRDGSFEVVMDRVFNPDEVYR

Sequences:

>Translated_141_residues
MSHVPLDPETARKVTSSLNYRFEIGGEPLVIAIAQDAENGDVLMTAFANEEAVYRTLTTGYAHYWSTSRREVWKKGEESG
HVQRVVEVRVDCDKDAVLYVVEQEGGACHTGYRSCFYRRVTRDGSFEVVMDRVFNPDEVYR
>Mature_140_residues
SHVPLDPETARKVTSSLNYRFEIGGEPLVIAIAQDAENGDVLMTAFANEEAVYRTLTTGYAHYWSTSRREVWKKGEESGH
VQRVVEVRVDCDKDAVLYVVEQEGGACHTGYRSCFYRRVTRDGSFEVVMDRVFNPDEVYR

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=88, Percent_Identity=39.7727272727273, Blast_Score=77, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319819, Length=71, Percent_Identity=39.4366197183099, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_METKA (P58836)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614820.1
- ProteinModelPortal:   P58836
- SMR:   P58836
- GeneID:   1478132
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1537
- NMPDR:   fig|190192.1.peg.1533
- HOGENOM:   HBG294308
- OMA:   SGHYQIV
- ProtClustDB:   PRK00051
- BioCyc:   MKAN190192:MK1537-MONOMER
- BRENDA:   3.5.4.19
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 16089; Mature: 15958

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVPLDPETARKVTSSLNYRFEIGGEPLVIAIAQDAENGDVLMTAFANEEAVYRTLTTG
CCCCCCCHHHHHHHHHHCCEEEEECCCEEEEEEECCCCCCCEEEEEECCCCHHHHHHHHC
YAHYWSTSRREVWKKGEESGHVQRVVEVRVDCDKDAVLYVVEQEGGACHTGYRSCFYRRV
HHHHHCCHHHHHHHCCCCCCHHEEEEEEEECCCCCEEEEEEECCCCEEEHHHHHHHHHHH
TRDGSFEVVMDRVFNPDEVYR
CCCCCCEEHEECCCCHHHHCC
>Mature Secondary Structure 
SHVPLDPETARKVTSSLNYRFEIGGEPLVIAIAQDAENGDVLMTAFANEEAVYRTLTTG
CCCCCCHHHHHHHHHHCCEEEEECCCEEEEEEECCCCCCCEEEEEECCCCHHHHHHHHC
YAHYWSTSRREVWKKGEESGHVQRVVEVRVDCDKDAVLYVVEQEGGACHTGYRSCFYRRV
HHHHHCCHHHHHHHCCCCCCHHEEEEEEEECCCCCEEEEEEECCCCEEEHHHHHHHHHHH
TRDGSFEVVMDRVFNPDEVYR
CCCCCCEEHEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014