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The map label for this gene is thiI [C]

Identifier: 20094898

GI number: 20094898

Start: 1489038

End: 1490084

Strand: Direct

Name: thiI [C]

Synonym: MK1462

Alternate gene names: 20094898

Gene position: 1489038-1490084 (Clockwise)

Preceding gene: 20094897

Following gene: 20094899

Centisome position: 87.85

GC content: 60.94

Gene sequence:

>1047_bases
ATGTTCCTAGTGAAAACGCAACGAAACCTGGAGGATGTAGCGGCAGCGATGATCGAGGAGGAAACCGGGGCCGAGGCCAA
GCCACGGCCCTTCGGTTACCTCGGTCTAGTGATCGTTACGGGACATGTTACCAAGGATGAACTCGAGAAGATCCCGGAGG
TAGAGCGGGCCATACCGGTGGAAGCCGAGTGCCGCGCGGATCCGAAGGAGATCGCCGAAACCGCGGCCGAACTGGCGGAG
ACCAAAATTTCGGAGGACGAGACCTTCGCGGTTAGGACGATCCGAAGGGGCGAACACGATTTCACCAGCGTCGACGTGAA
CGTTGAGGCCGGCGACGCCGTCCGGAAGGCCACCGGTGCCTCCGTGGACCTCGACGATCCCGACAAGATAGTGTGGGTCG
AGATACTCAGAGACCGGGCGTACCTAGCGGTCTTACCCGGCGAGGAGGAGTGGAAGAAGCTACCTCCAGGAAAACCCGAA
GCCGATCCACTGCTCGCCAAACTCGAGTTAGCACAGATACCGTATCTCGGGGATCCACGGGCCGCCTACTCCATGGGTGA
AAGGATCGGGCGGGCGGTTCAGGGGTTCGAATTGAAGTCGTACATCGTCACCCCTTACGAACCGGTGAACGCCGTGGAAT
TGCTGCACTTCTTACGGGGTCTACGAGATGGCGTGAAGTCCAGGATGGACGTACAACGCGAGTCGTACGGGCGGGAGTTC
CGGCGGACGGAGCTGCTACTCTACGACCTCTACCAGCTGATACGGCTGAAGCGGGACGCCCTGATCATAGGGACGGATCC
GAAGGGCGATCCGTACACGGAGATCCGGAAGACACTGGGCGAAGCACTCCGGGAGGCGGACGAGGTGGTCGTGCTGGCGG
GATCGCGGGTGGGATTACCTAGGGGAGTGCTGAGAGCGTGTGACTTCGTCGTAGACCTGTGTCCGGGAGTGACGTTCGCG
ACGGAACACGTCGTACCCTCCGTCGTGACGGCGCTGGTGGACTCGTACCTGGAGGCCGAAGGGAGCGAAGATCGAGAAGG
ACGTTGA

Upstream 100 bases:

>100_bases
AACGGCCTACCGCCATACGAAGCGGTGGGGGACCGGGCCACCCCGGAGCTGGCCAGCTACTGAGCCTCCCTCCGCAACGG
TCTTCGGGCGCGTGCGACGG

Downstream 100 bases:

>100_bases
ACGCACATCTCTGAACGATACCGAGTTCGATAATAAGCGATCATACAGAATGCGCACAGTAGGTAGGATTCGATCTGAAG
ACACCAACTTATCGTTGGAT

Product: RNA-binding protein

Products: ThiS-COSH; AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MFLVKTQRNLEDVAAAMIEEETGAEAKPRPFGYLGLVIVTGHVTKDELEKIPEVERAIPVEAECRADPKEIAETAAELAE
TKISEDETFAVRTIRRGEHDFTSVDVNVEAGDAVRKATGASVDLDDPDKIVWVEILRDRAYLAVLPGEEEWKKLPPGKPE
ADPLLAKLELAQIPYLGDPRAAYSMGERIGRAVQGFELKSYIVTPYEPVNAVELLHFLRGLRDGVKSRMDVQRESYGREF
RRTELLLYDLYQLIRLKRDALIIGTDPKGDPYTEIRKTLGEALREADEVVVLAGSRVGLPRGVLRACDFVVDLCPGVTFA
TEHVVPSVVTALVDSYLEAEGSEDREGR

Sequences:

>Translated_348_residues
MFLVKTQRNLEDVAAAMIEEETGAEAKPRPFGYLGLVIVTGHVTKDELEKIPEVERAIPVEAECRADPKEIAETAAELAE
TKISEDETFAVRTIRRGEHDFTSVDVNVEAGDAVRKATGASVDLDDPDKIVWVEILRDRAYLAVLPGEEEWKKLPPGKPE
ADPLLAKLELAQIPYLGDPRAAYSMGERIGRAVQGFELKSYIVTPYEPVNAVELLHFLRGLRDGVKSRMDVQRESYGREF
RRTELLLYDLYQLIRLKRDALIIGTDPKGDPYTEIRKTLGEALREADEVVVLAGSRVGLPRGVLRACDFVVDLCPGVTFA
TEHVVPSVVTALVDSYLEAEGSEDREGR
>Mature_348_residues
MFLVKTQRNLEDVAAAMIEEETGAEAKPRPFGYLGLVIVTGHVTKDELEKIPEVERAIPVEAECRADPKEIAETAAELAE
TKISEDETFAVRTIRRGEHDFTSVDVNVEAGDAVRKATGASVDLDDPDKIVWVEILRDRAYLAVLPGEEEWKKLPPGKPE
ADPLLAKLELAQIPYLGDPRAAYSMGERIGRAVQGFELKSYIVTPYEPVNAVELLHFLRGLRDGVKSRMDVQRESYGREF
RRTELLLYDLYQLIRLKRDALIIGTDPKGDPYTEIRKTLGEALREADEVVVLAGSRVGLPRGVLRACDFVVDLCPGVTFA
TEHVVPSVVTALVDSYLEAEGSEDREGR

Specific function: Required For The Synthesis Of The Thiazole Moiety. [C]

COG id: COG1818

COG function: function code R; Predicted RNA-binding protein, contains THUMP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004114 [H]

Pfam domain/function: PF02926 THUMP [H]

EC number: NA

Molecular weight: Translated: 38672; Mature: 38672

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLVKTQRNLEDVAAAMIEEETGAEAKPRPFGYLGLVIVTGHVTKDELEKIPEVERAIPV
CEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHCCHHHHHCCC
EAECRADPKEIAETAAELAETKISEDETFAVRTIRRGEHDFTSVDVNVEAGDAVRKATGA
CHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCC
SVDLDDPDKIVWVEILRDRAYLAVLPGEEEWKKLPPGKPEADPLLAKLELAQIPYLGDPR
CCCCCCCCCEEHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCH
AAYSMGERIGRAVQGFELKSYIVTPYEPVNAVELLHFLRGLRDGVKSRMDVQRESYGREF
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRTELLLYDLYQLIRLKRDALIIGTDPKGDPYTEIRKTLGEALREADEVVVLAGSRVGLP
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCC
RGVLRACDFVVDLCPGVTFATEHVVPSVVTALVDSYLEAEGSEDREGR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFLVKTQRNLEDVAAAMIEEETGAEAKPRPFGYLGLVIVTGHVTKDELEKIPEVERAIPV
CEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHCCHHHHHCCC
EAECRADPKEIAETAAELAETKISEDETFAVRTIRRGEHDFTSVDVNVEAGDAVRKATGA
CHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCC
SVDLDDPDKIVWVEILRDRAYLAVLPGEEEWKKLPPGKPEADPLLAKLELAQIPYLGDPR
CCCCCCCCCEEHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCH
AAYSMGERIGRAVQGFELKSYIVTPYEPVNAVELLHFLRGLRDGVKSRMDVQRESYGREF
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRTELLLYDLYQLIRLKRDALIIGTDPKGDPYTEIRKTLGEALREADEVVVLAGSRVGLP
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCC
RGVLRACDFVVDLCPGVTFATEHVVPSVVTALVDSYLEAEGSEDREGR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]