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The map label for this gene is Nfi

Identifier: 20094871

GI number: 20094871

Start: 1463867

End: 1464556

Strand: Direct

Name: Nfi

Synonym: MK1435

Alternate gene names: 20094871

Gene position: 1463867-1464556 (Clockwise)

Preceding gene: 20094870

Following gene: 20094872

Centisome position: 86.37

GC content: 65.22

Gene sequence:

>690_bases
GTGAAAAGCGCTGAATGGTTCGAGGCCAGCACCGAGGAGGAGCGCGTAGAAATTCAGCGGAAGGTAGCCCGTAAGGTCCG
ACTGGAACCGCTCGACGACGTGGACGCCGTCGCTGGGGTAGACGTCTCTTACCGCGGTGAAGAGTACCGGGCCGCCGCGG
TGGTTCTGGACCCCGAAACCTACGAAGTGCTCGACCGTCGAGTGGTCCACGGGACTACGGACGTCCCCTACGAGCCCGGG
TTCCTGGCGTTCCGTGAGGGTCCACCGGCCCTGGAGGCTCTCGAGGGACTGGACTTCGACCTGCTGTTCGTCCACGGCCA
CGGCGTAGCCCACCCTAGGCGGGCCGGTCTCGCTTCCCACCTCGGAGTGGCCCTAGACGTCCCGACGATAGGGGTGGCGC
GGCGTCCGCTCGTGGGCAGGTCGAAGGAGGAACCGAGTAGGATAGGCGATACCACACCCCTCGTACATAGAGGTGAGGTA
GTCGGCTACCTCGTACGAACCGACGCCGAAGCCCGGCCCGTAGTGGTGAGCCCGGGCCATCGGTGTAACCTCGAGGACGC
CGTGAGATGGACGCTGCGCCTCGTCCGAGTGGGAAAGTGGCCAGAGCCGCTGCGTCTGGCCGATCTGCTCTCACGTAGAG
GAGCATCACGGGTAGAGGGTGAAAGTCGTGGCGCTGGTGTACGACGCTGA

Upstream 100 bases:

>100_bases
GTCCCCAGGAAAGCTGGCAAGAACGTCTACGTGGTGGAAGGAACGGTGCCTCCGGACTGCATCACTCGCGTGATCCGGAA
CCCAAGGAGGTCCGTCGAGA

Downstream 100 bases:

>100_bases
GTTCGTGGGCTCGGAGCGAGAGTTCGAGGAGGAACGCGAGACGTTCCTCAAGGGTGTCAAGGCCTACGACGGCGTTCTGG
CCACCCGTTACTTGATGGAG

Product: deoxyinosine 3'endonuclease (endonuclease V)

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKSAEWFEASTEEERVEIQRKVARKVRLEPLDDVDAVAGVDVSYRGEEYRAAAVVLDPETYEVLDRRVVHGTTDVPYEPG
FLAFREGPPALEALEGLDFDLLFVHGHGVAHPRRAGLASHLGVALDVPTIGVARRPLVGRSKEEPSRIGDTTPLVHRGEV
VGYLVRTDAEARPVVVSPGHRCNLEDAVRWTLRLVRVGKWPEPLRLADLLSRRGASRVEGESRGAGVRR

Sequences:

>Translated_229_residues
MKSAEWFEASTEEERVEIQRKVARKVRLEPLDDVDAVAGVDVSYRGEEYRAAAVVLDPETYEVLDRRVVHGTTDVPYEPG
FLAFREGPPALEALEGLDFDLLFVHGHGVAHPRRAGLASHLGVALDVPTIGVARRPLVGRSKEEPSRIGDTTPLVHRGEV
VGYLVRTDAEARPVVVSPGHRCNLEDAVRWTLRLVRVGKWPEPLRLADLLSRRGASRVEGESRGAGVRR
>Mature_229_residues
MKSAEWFEASTEEERVEIQRKVARKVRLEPLDDVDAVAGVDVSYRGEEYRAAAVVLDPETYEVLDRRVVHGTTDVPYEPG
FLAFREGPPALEALEGLDFDLLFVHGHGVAHPRRAGLASHLGVALDVPTIGVARRPLVGRSKEEPSRIGDTTPLVHRGEV
VGYLVRTDAEARPVVVSPGHRCNLEDAVRWTLRLVRVGKWPEPLRLADLLSRRGASRVEGESRGAGVRR

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=198, Percent_Identity=40.9090909090909, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI257467548, Length=135, Percent_Identity=40, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI257467550, Length=135, Percent_Identity=40, Blast_Score=74, Evalue=9e-14,
Organism=Escherichia coli, GI87082357, Length=204, Percent_Identity=41.1764705882353, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_METKA (Q8X260)

Other databases:

- EMBL:   AF311944
- EMBL:   AE009439
- RefSeq:   NP_614718.1
- ProteinModelPortal:   Q8X260
- GeneID:   1478030
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1435
- NMPDR:   fig|190192.1.peg.1431
- HOGENOM:   HBG716360
- OMA:   CMRGYRL
- BioCyc:   MKAN190192:MK1435-MONOMER
- BRENDA:   3.1.21.7
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 25331; Mature: 25331

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSAEWFEASTEEERVEIQRKVARKVRLEPLDDVDAVAGVDVSYRGEEYRAAAVVLDPET
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCEEEEEEEECCHH
YEVLDRRVVHGTTDVPYEPGFLAFREGPPALEALEGLDFDLLFVHGHGVAHPRRAGLASH
HHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHCCCEEEEEEECCCCCCCHHHCHHHH
LGVALDVPTIGVARRPLVGRSKEEPSRIGDTTPLVHRGEVVGYLVRTDAEARPVVVSPGH
CCEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCEECCCEEEEEEECCCCCCCEEECCCC
RCNLEDAVRWTLRLVRVGKWPEPLRLADLLSRRGASRVEGESRGAGVRR
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSAEWFEASTEEERVEIQRKVARKVRLEPLDDVDAVAGVDVSYRGEEYRAAAVVLDPET
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCEEEEEEEECCHH
YEVLDRRVVHGTTDVPYEPGFLAFREGPPALEALEGLDFDLLFVHGHGVAHPRRAGLASH
HHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHCCCEEEEEEECCCCCCCHHHCHHHH
LGVALDVPTIGVARRPLVGRSKEEPSRIGDTTPLVHRGEVVGYLVRTDAEARPVVVSPGH
CCEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCEECCCEEEEEEECCCCCCCEEECCCC
RCNLEDAVRWTLRLVRVGKWPEPLRLADLLSRRGASRVEGESRGAGVRR
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11353838; 11930014