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The map label for this gene is tuf [H]

Identifier: 20094840

GI number: 20094840

Start: 1428233

End: 1429309

Strand: Reverse

Name: tuf [H]

Synonym: MK1404

Alternate gene names: 20094840

Gene position: 1429309-1428233 (Counterclockwise)

Preceding gene: 20094841

Following gene: 20094839

Centisome position: 84.33

GC content: 67.69

Gene sequence:

>1077_bases
GTGGAGCGACTGGAGGGTGGCGGCGTGAGTGTCGTCAACGTGGTAGTCTGCGGACATTTCAACCACGGAAAGACCACGCT
CGTGAAGGCGCTGACCGGTGAGTGGTTGGACAGGCTACCCCACGAGCGGGAGATGGGGGTCACGATAGAGCCCGCACGCG
CGTTCCTGGAGCTCGGCGATACCACGGTCTCGTTCGTGGACGTACCGGGTCATCGTGACTACATCCGCAACATGCTGGCG
TCCGCCTGGAGCGCGGATTACGCGATCCTGGTGGTCGCGGCCGACGAGGGACCGTGTCCAGGAACGATCGACCACGCGCT
CGTGGTCTCGTTCTACGGGGCGAGAGTACTACCCGTCGTGAGCAAGGTGGACCTGGTAAGCCGCGATCGGGCGGCCGAGG
TCGCGGATGAGGTGATGGATCTTCTGGAGCTGCTCGGTGTGGAAGCCGTCGTGGAACCCGTTCCGGTGTCCGCGAAGACG
GGGGAAGGGGCGGAAGAGCTGCTCGACGCGCTGGCCGAGCTGGAGGAGCCACCTCGACCGTCCGAGATGGACCCGCTGCG
GGCGCCGGTGGAGTCCACGAGGAGGGTGGACGAGACGACCGTGGACGTGTTCGGGATCGTGGATCGGGGTACGCTGGAGG
AAGGGCGGGTCGAAGTCCAGCCGGGTGGACGGAGCGCGGAGGTGTTGGAGGTGGAATCGACGGACGGATCCGGGCCGGGG
GTTCCGTTCCGGGCCAGGCTGAGGGTGAAGGGCTCGATCACGCGCGGGATGTGCCTCGGGGAGGGGTTGTCGACCGCCGA
CCGGATCGAGGCGGAAGTTCTCTCGATCGGCGCTAAGGTCCGGCCCGGGACGATGGGGAAGGTGCACGTGCTCTCCGCGG
CCGCGAATGTCAGGTTCGCGGAGGTCGAGACGGACGCCGGGACGGAAGTGCTGCGACCCTACGCCGAGGGCCACAACGTG
GCGCACGTCGTCCTGGAGCTGGACCGTGAGGTGGTGGTGGAGCCCGGCGACCGGTTCCTGGTGACCGTGGGCAACGAGCC
GGCGGTGCTCGGTGTCGTCGAGGGGGTGGCGCGTTGA

Upstream 100 bases:

>100_bases
TTTCTGGCCGTGTGGTGGGCGCGGCGCTACGAGCCGGGCCCTCGGGGGTGGTAAATAAAAGGCGATTCGCGGGATTAACC
GCCCGGAGGCTCAAGGGCGA

Downstream 100 bases:

>100_bases
GCTGCGGGGAAGACAAGGAAGTCCTGTTCGAAGCTCTGGACGCCGTCGAGGAGAAGTACGACCTGAGGAAGTCGCAGTAC
GAGATGTTGGAAGAAATCCG

Product: translation elongation factor, GTPase

Products: NA

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MERLEGGGVSVVNVVVCGHFNHGKTTLVKALTGEWLDRLPHEREMGVTIEPARAFLELGDTTVSFVDVPGHRDYIRNMLA
SAWSADYAILVVAADEGPCPGTIDHALVVSFYGARVLPVVSKVDLVSRDRAAEVADEVMDLLELLGVEAVVEPVPVSAKT
GEGAEELLDALAELEEPPRPSEMDPLRAPVESTRRVDETTVDVFGIVDRGTLEEGRVEVQPGGRSAEVLEVESTDGSGPG
VPFRARLRVKGSITRGMCLGEGLSTADRIEAEVLSIGAKVRPGTMGKVHVLSAAANVRFAEVETDAGTEVLRPYAEGHNV
AHVVLELDREVVVEPGDRFLVTVGNEPAVLGVVEGVAR

Sequences:

>Translated_358_residues
MERLEGGGVSVVNVVVCGHFNHGKTTLVKALTGEWLDRLPHEREMGVTIEPARAFLELGDTTVSFVDVPGHRDYIRNMLA
SAWSADYAILVVAADEGPCPGTIDHALVVSFYGARVLPVVSKVDLVSRDRAAEVADEVMDLLELLGVEAVVEPVPVSAKT
GEGAEELLDALAELEEPPRPSEMDPLRAPVESTRRVDETTVDVFGIVDRGTLEEGRVEVQPGGRSAEVLEVESTDGSGPG
VPFRARLRVKGSITRGMCLGEGLSTADRIEAEVLSIGAKVRPGTMGKVHVLSAAANVRFAEVETDAGTEVLRPYAEGHNV
AHVVLELDREVVVEPGDRFLVTVGNEPAVLGVVEGVAR
>Mature_358_residues
MERLEGGGVSVVNVVVCGHFNHGKTTLVKALTGEWLDRLPHEREMGVTIEPARAFLELGDTTVSFVDVPGHRDYIRNMLA
SAWSADYAILVVAADEGPCPGTIDHALVVSFYGARVLPVVSKVDLVSRDRAAEVADEVMDLLELLGVEAVVEPVPVSAKT
GEGAEELLDALAELEEPPRPSEMDPLRAPVESTRRVDETTVDVFGIVDRGTLEEGRVEVQPGGRSAEVLEVESTDGSGPG
VPFRARLRVKGSITRGMCLGEGLSTADRIEAEVLSIGAKVRPGTMGKVHVLSAAANVRFAEVETDAGTEVLRPYAEGHNV
AHVVLELDREVVVEPGDRFLVTVGNEPAVLGVVEGVAR

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=230, Percent_Identity=31.7391304347826, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI4503471, Length=279, Percent_Identity=29.3906810035842, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4503475, Length=279, Percent_Identity=29.3906810035842, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157426893, Length=226, Percent_Identity=29.2035398230088, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI223555963, Length=262, Percent_Identity=27.8625954198473, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI5729864, Length=262, Percent_Identity=27.8625954198473, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI194018522, Length=295, Percent_Identity=27.1186440677966, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI194018520, Length=354, Percent_Identity=25.9887005649718, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI194097354, Length=354, Percent_Identity=25.9887005649718, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI46094014, Length=295, Percent_Identity=27.1186440677966, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI2367247, Length=208, Percent_Identity=35.0961538461538, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790412, Length=397, Percent_Identity=27.2040302267003, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789737, Length=397, Percent_Identity=27.2040302267003, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789108, Length=273, Percent_Identity=26.7399267399267, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788922, Length=179, Percent_Identity=30.1675977653631, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=362, Percent_Identity=29.5580110497238, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=27.1356783919598, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17552884, Length=279, Percent_Identity=29.0322580645161, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17569207, Length=279, Percent_Identity=29.0322580645161, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115532067, Length=276, Percent_Identity=28.9855072463768, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI115532065, Length=276, Percent_Identity=28.9855072463768, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=220, Percent_Identity=26.8181818181818, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566629, Length=398, Percent_Identity=23.1155778894472, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17506081, Length=221, Percent_Identity=28.9592760180996, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=233, Percent_Identity=33.9055793991416, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6325337, Length=287, Percent_Identity=29.616724738676, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319594, Length=287, Percent_Identity=29.616724738676, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=195, Percent_Identity=30.2564102564103, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921738, Length=226, Percent_Identity=31.858407079646, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281363316, Length=390, Percent_Identity=27.9487179487179, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17864358, Length=390, Percent_Identity=27.9487179487179, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45553807, Length=279, Percent_Identity=30.8243727598566, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45553816, Length=279, Percent_Identity=30.8243727598566, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24651721, Length=279, Percent_Identity=30.8243727598566, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17864154, Length=279, Percent_Identity=30.8243727598566, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652838, Length=287, Percent_Identity=29.2682926829268, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17137572, Length=287, Percent_Identity=29.2682926829268, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45550900, Length=263, Percent_Identity=28.5171102661597, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922690, Length=224, Percent_Identity=29.4642857142857, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17137380, Length=298, Percent_Identity=27.8523489932886, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 38159; Mature: 38159

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLEGGGVSVVNVVVCGHFNHGKTTLVKALTGEWLDRLPHEREMGVTIEPARAFLELGD
CCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCC
TTVSFVDVPGHRDYIRNMLASAWSADYAILVVAADEGPCPGTIDHALVVSFYGARVLPVV
CEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHH
SKVDLVSRDRAAEVADEVMDLLELLGVEAVVEPVPVSAKTGEGAEELLDALAELEEPPRP
HHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
SEMDPLRAPVESTRRVDETTVDVFGIVDRGTLEEGRVEVQPGGRSAEVLEVESTDGSGPG
CCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCCCEEEECCCCCCEEEEEEECCCCCCCC
VPFRARLRVKGSITRGMCLGEGLSTADRIEAEVLSIGAKVRPGTMGKVHVLSAAANVRFA
CCEEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCEECCCCCCEEEEEEHHCCEEEE
EVETDAGTEVLRPYAEGHNVAHVVLELDREVVVEPGDRFLVTVGNEPAVLGVVEGVAR
EEECCCCHHHHHHHHCCCCEEEEEEEECCEEEECCCCEEEEEECCCCCEEEHHHHHCC
>Mature Secondary Structure
MERLEGGGVSVVNVVVCGHFNHGKTTLVKALTGEWLDRLPHEREMGVTIEPARAFLELGD
CCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCC
TTVSFVDVPGHRDYIRNMLASAWSADYAILVVAADEGPCPGTIDHALVVSFYGARVLPVV
CEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHH
SKVDLVSRDRAAEVADEVMDLLELLGVEAVVEPVPVSAKTGEGAEELLDALAELEEPPRP
HHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
SEMDPLRAPVESTRRVDETTVDVFGIVDRGTLEEGRVEVQPGGRSAEVLEVESTDGSGPG
CCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCCCEEEECCCCCCEEEEEEECCCCCCCC
VPFRARLRVKGSITRGMCLGEGLSTADRIEAEVLSIGAKVRPGTMGKVHVLSAAANVRFA
CCEEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCEECCCCCCEEEEEEHHCCEEEE
EVETDAGTEVLRPYAEGHNVAHVVLELDREVVVEPGDRFLVTVGNEPAVLGVVEGVAR
EEECCCCHHHHHHHHCCCCEEEEEEEECCEEEECCCCEEEEEECCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]