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The map label for this gene is PRI2

Identifier: 20094830

GI number: 20094830

Start: 1419851

End: 1421071

Strand: Direct

Name: PRI2

Synonym: MK1394

Alternate gene names: 20094830

Gene position: 1419851-1421071 (Clockwise)

Preceding gene: 20094828

Following gene: 20094837

Centisome position: 83.77

GC content: 63.55

Gene sequence:

>1221_bases
TTGCGCTCATGGTTCCCCCACGCCCCGACGGTATCCGTTCCGGAGATAGAACCGGAAGAGGCCCTGTCGTACCGAGACGA
GATCCGAACCAGGGTCAGATCCTACGCCTTCGGGCTGGACGACTGGCGGGCGAAGGTAAAGACGCGTCGCGCTTACCTGC
GCAAGGTCAGGGACGAGGACGAGTCGGAGATCCTGCGAGTCTACGGGGCGTTACTCACCGTGGCGGCCGCCGGACCGCGT
CCCGTCCGTCATCTGATAGCCGAAGGCGAGTCTGCGATGGCCGAGACCGCGCTCTACGCCCTTCGCCACGAGGATGAGTC
CGCAATGTCCTACCCCGAGGAGCGTGTTCTCAGCGACCTGTACCTGTGGGACGTACGGGTACTCGAGCGCGACCTGGGTC
TGTACGCCGTCCACTTCTCGTTTCCACTCATACACCGATCCCCGGAAGGACAGAAGCTGAAGGTGCTGGTGTGGGAACGC
GCCTCTTTGGAGAAGGTACTGAAGGAGGTCAGGTCGAACAGGGTCCTCGGAGTGAGGGTTCTCGATCCCTCGGGATGGAA
CGACGTGTGGATATGCCCGCGGTGCGGGGCCACGAGGCGGGGAGGGACGGGAGACCTGGAGCAGGACCACGCCCGCCGGT
GCTCCAACTGCCGAGGGCGAATGCGGAAGCCGGACCCTAACCTGCTCGAGATGCTCAAGGAGCCGGAAGGGTACCTGATC
ATGCACTTCAAGACCTTGGCCCGGACGTTCCGCGAGGACGTCCGTAGATTGGTGGTCTCGGATATCGAGTCCCGGGACGA
CGTGGAGGACGAAGAGCTGCGGGAGTTCTGCCTCAAACTGTTCCCGAAGGTTCGGAAGCGTCTGGAACGCGTCGAGAAGG
GGGCGGGCGGCCGGTTCCCGCCGTGTATCCGGGAGCTCTTACGGAGGGCCCAAGAAGGGGAGAACTTGCCCCACGAGGCC
CGCTTCGCGCTGGCCGCCTTCCTGGTCAACGTGGGATGGGACGTGGACCGCGTGGTGGAAGTGTTCTCGAACCTGCCAGA
CTTCGACGAGGAGCGCACCCAGTACCAGGTCCGTCACATCGCCGGTGAGGTGGGCGGAGGTACTCGATATCTACCACCGA
ACTGCGATAAGATGAAAGCATGGGGGTTGTGCCCCGGCAAGGATTGTGGGGTGAAGAATCCGCTGGCGTACTACCGCCGA
CCTAGGGCCGACGACGGATGA

Upstream 100 bases:

>100_bases
AACCCTTCGGGTAGTACCCTACGCACCACGGGCTCGAGGGCACGACCCTCGTCCCCGTGAACCCCGTACACTGCCACGGG
TCTGCGGATGGGGTGGTCGT

Downstream 100 bases:

>100_bases
ACCGTTATGCGGACTGGAGGCACCCCGAGCTCGTCCGCCAACGTTTCGAGCTTCTCCTCGACCTCTTTCACGCGGCTGCC
GTCCTTGCCGATCGCCTTGT

Product: DNA primase large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MRSWFPHAPTVSVPEIEPEEALSYRDEIRTRVRSYAFGLDDWRAKVKTRRAYLRKVRDEDESEILRVYGALLTVAAAGPR
PVRHLIAEGESAMAETALYALRHEDESAMSYPEERVLSDLYLWDVRVLERDLGLYAVHFSFPLIHRSPEGQKLKVLVWER
ASLEKVLKEVRSNRVLGVRVLDPSGWNDVWICPRCGATRRGGTGDLEQDHARRCSNCRGRMRKPDPNLLEMLKEPEGYLI
MHFKTLARTFREDVRRLVVSDIESRDDVEDEELREFCLKLFPKVRKRLERVEKGAGGRFPPCIRELLRRAQEGENLPHEA
RFALAAFLVNVGWDVDRVVEVFSNLPDFDEERTQYQVRHIAGEVGGGTRYLPPNCDKMKAWGLCPGKDCGVKNPLAYYRR
PRADDG

Sequences:

>Translated_406_residues
MRSWFPHAPTVSVPEIEPEEALSYRDEIRTRVRSYAFGLDDWRAKVKTRRAYLRKVRDEDESEILRVYGALLTVAAAGPR
PVRHLIAEGESAMAETALYALRHEDESAMSYPEERVLSDLYLWDVRVLERDLGLYAVHFSFPLIHRSPEGQKLKVLVWER
ASLEKVLKEVRSNRVLGVRVLDPSGWNDVWICPRCGATRRGGTGDLEQDHARRCSNCRGRMRKPDPNLLEMLKEPEGYLI
MHFKTLARTFREDVRRLVVSDIESRDDVEDEELREFCLKLFPKVRKRLERVEKGAGGRFPPCIRELLRRAQEGENLPHEA
RFALAAFLVNVGWDVDRVVEVFSNLPDFDEERTQYQVRHIAGEVGGGTRYLPPNCDKMKAWGLCPGKDCGVKNPLAYYRR
PRADDG
>Mature_406_residues
MRSWFPHAPTVSVPEIEPEEALSYRDEIRTRVRSYAFGLDDWRAKVKTRRAYLRKVRDEDESEILRVYGALLTVAAAGPR
PVRHLIAEGESAMAETALYALRHEDESAMSYPEERVLSDLYLWDVRVLERDLGLYAVHFSFPLIHRSPEGQKLKVLVWER
ASLEKVLKEVRSNRVLGVRVLDPSGWNDVWICPRCGATRRGGTGDLEQDHARRCSNCRGRMRKPDPNLLEMLKEPEGYLI
MHFKTLARTFREDVRRLVVSDIESRDDVEDEELREFCLKLFPKVRKRLERVEKGAGGRFPPCIRELLRRAQEGENLPHEA
RFALAAFLVNVGWDVDRVVEVFSNLPDFDEERTQYQVRHIAGEVGGGTRYLPPNCDKMKAWGLCPGKDCGVKNPLAYYRR
PRADDG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG2219

COG function: function code L; Eukaryotic-type DNA primase, large subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase large subunit family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIL_METKA (Q8TVJ5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614677.1
- GeneID:   1477989
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1394
- NMPDR:   fig|190192.1.peg.1390
- ProtClustDB:   PRK02249
- BioCyc:   MKAN190192:MK1394-MONOMER
- HAMAP:   MF_00701
- InterPro:   IPR007238
- PANTHER:   PTHR10537

Pfam domain/function: PF04104 DNA_primase_lrg

EC number: NA

Molecular weight: Translated: 46873; Mature: 46873

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSWFPHAPTVSVPEIEPEEALSYRDEIRTRVRSYAFGLDDWRAKVKTRRAYLRKVRDED
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
ESEILRVYGALLTVAAAGPRPVRHLIAEGESAMAETALYALRHEDESAMSYPEERVLSDL
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
YLWDVRVLERDLGLYAVHFSFPLIHRSPEGQKLKVLVWERASLEKVLKEVRSNRVLGVRV
HHHHHHHHHHCCCEEEEEEECHHEECCCCCCEEEEEEECHHHHHHHHHHHHCCCEEEEEE
LDPSGWNDVWICPRCGATRRGGTGDLEQDHARRCSNCRGRMRKPDPNLLEMLKEPEGYLI
ECCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEE
MHFKTLARTFREDVRRLVVSDIESRDDVEDEELREFCLKLFPKVRKRLERVEKGAGGRFP
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PCIRELLRRAQEGENLPHEARFALAAFLVNVGWDVDRVVEVFSNLPDFDEERTQYQVRHI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
AGEVGGGTRYLPPNCDKMKAWGLCPGKDCGVKNPLAYYRRPRADDG
HHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MRSWFPHAPTVSVPEIEPEEALSYRDEIRTRVRSYAFGLDDWRAKVKTRRAYLRKVRDED
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
ESEILRVYGALLTVAAAGPRPVRHLIAEGESAMAETALYALRHEDESAMSYPEERVLSDL
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
YLWDVRVLERDLGLYAVHFSFPLIHRSPEGQKLKVLVWERASLEKVLKEVRSNRVLGVRV
HHHHHHHHHHCCCEEEEEEECHHEECCCCCCEEEEEEECHHHHHHHHHHHHCCCEEEEEE
LDPSGWNDVWICPRCGATRRGGTGDLEQDHARRCSNCRGRMRKPDPNLLEMLKEPEGYLI
ECCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEE
MHFKTLARTFREDVRRLVVSDIESRDDVEDEELREFCLKLFPKVRKRLERVEKGAGGRFP
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PCIRELLRRAQEGENLPHEARFALAAFLVNVGWDVDRVVEVFSNLPDFDEERTQYQVRHI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
AGEVGGGTRYLPPNCDKMKAWGLCPGKDCGVKNPLAYYRRPRADDG
HHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014