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The map label for this gene is CobM [H]

Identifier: 20094811

GI number: 20094811

Start: 1402137

End: 1402895

Strand: Direct

Name: CobM [H]

Synonym: MK1375

Alternate gene names: 20094811

Gene position: 1402137-1402895 (Clockwise)

Preceding gene: 20094810

Following gene: 20094812

Centisome position: 82.72

GC content: 61.92

Gene sequence:

>759_bases
ATGTCCGTGACGAAGGTCTACTTCGTCGGCGCCGGTCCGGGCGACCCCGAGCTGATGACGTTGAAGGGTGTACGGGTGCT
TCGACGGGCTGACCTCGTGATATACCCCGGTTCGCTGATTCCTCGTGAGTCTGTGGAGGAATGGGCCCCGAACGCCGAGC
TCATCGACAGTCACGGCAAGACACTGGAGGAGCTGGTGGAGACCATGGTCGAAGCCGTGGAGGACAGCCGTACCGTGGTA
CGACTAGTTTCGGGAGATCCCTTCGTCTACAGCTCACTGTACGAACAAGTCCGCGAACTCAGACGACGGGGAGTGGATTA
CGAGGTGATCCCCGGCGTAAGCTCCGTCAACGCCGCCGCAGCGGCACTAGGTGAGGAGCTGACGAAACCCGGGATCAGTC
AGACGGTGATACTCACCCGCCCGGCCGGGAGGACCGGGAAGCCCGAAGGTGAGTCGCTTTCCGAGCTCGCGGAGCACGGT
TGCACGATGGTGATCTTCCTGGGGGCCGCTTACCTGGAGCGCATCGTGAAGTCACTGCTCGGAGGCGCGTACGACGAGGA
TACCCCGGCCGCCGTCGTGTACAAGGCGTCCACACCCGAGGAGAAGGTGATCCTGGGTACGCTAGGAGACATCGCTGAGA
AGGCGCGTGAGGAGGGTATCGATAGGACCGCCCTGATCATCGTGGGTGACGTCTTGAAGGAGGAGGGGAAGAGGTCTCAC
TTATACTCGGAGGAGTACGCGAGGAGGGTGCGGAGATGA

Upstream 100 bases:

>100_bases
GTTTGGTCGTGGTCGGAGCCATCGCGTCGATGACACGGCGCATCCGCGTGTAGTTCCCTCTCCCCTCACCCATCGTTAAG
AGTGACGTAACGTTAGGGGG

Downstream 100 bases:

>100_bases
CGGGGCTGGGATTCTCGCCGAAACGTCGTCTAGACCGCGTCGTGGTCTACGAGTTCCGGGATCTCCCGGTGCTCGACGAC
GTTCTGGAGTTCTTCGAGCT

Product: precorrin-4 methylase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSVTKVYFVGAGPGDPELMTLKGVRVLRRADLVIYPGSLIPRESVEEWAPNAELIDSHGKTLEELVETMVEAVEDSRTVV
RLVSGDPFVYSSLYEQVRELRRRGVDYEVIPGVSSVNAAAAALGEELTKPGISQTVILTRPAGRTGKPEGESLSELAEHG
CTMVIFLGAAYLERIVKSLLGGAYDEDTPAAVVYKASTPEEKVILGTLGDIAEKAREEGIDRTALIIVGDVLKEEGKRSH
LYSEEYARRVRR

Sequences:

>Translated_252_residues
MSVTKVYFVGAGPGDPELMTLKGVRVLRRADLVIYPGSLIPRESVEEWAPNAELIDSHGKTLEELVETMVEAVEDSRTVV
RLVSGDPFVYSSLYEQVRELRRRGVDYEVIPGVSSVNAAAAALGEELTKPGISQTVILTRPAGRTGKPEGESLSELAEHG
CTMVIFLGAAYLERIVKSLLGGAYDEDTPAAVVYKASTPEEKVILGTLGDIAEKAREEGIDRTALIIVGDVLKEEGKRSH
LYSEEYARRVRR
>Mature_251_residues
SVTKVYFVGAGPGDPELMTLKGVRVLRRADLVIYPGSLIPRESVEEWAPNAELIDSHGKTLEELVETMVEAVEDSRTVVR
LVSGDPFVYSSLYEQVRELRRRGVDYEVIPGVSSVNAAAAALGEELTKPGISQTVILTRPAGRTGKPEGESLSELAEHGC
TMVIFLGAAYLERIVKSLLGGAYDEDTPAAVVYKASTPEEKVILGTLGDIAEKAREEGIDRTALIIVGDVLKEEGKRSHL
YSEEYARRVRR

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=236, Percent_Identity=29.2372881355932, Blast_Score=94, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6322922, Length=232, Percent_Identity=26.2931034482759, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27488; Mature: 27356

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTKVYFVGAGPGDPELMTLKGVRVLRRADLVIYPGSLIPRESVEEWAPNAELIDSHGK
CCCEEEEEEECCCCCCCEEEHHHHHHHHHCCEEEECCCCCCHHHHHHHCCCHHHHHHCCH
TLEELVETMVEAVEDSRTVVRLVSGDPFVYSSLYEQVRELRRRGVDYEVIPGVSSVNAAA
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHH
AALGEELTKPGISQTVILTRPAGRTGKPEGESLSELAEHGCTMVIFLGAAYLERIVKSLL
HHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHH
GGAYDEDTPAAVVYKASTPEEKVILGTLGDIAEKAREEGIDRTALIIVGDVLKEEGKRSH
CCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCHHC
LYSEEYARRVRR
CHHHHHHHHHCC
>Mature Secondary Structure 
SVTKVYFVGAGPGDPELMTLKGVRVLRRADLVIYPGSLIPRESVEEWAPNAELIDSHGK
CCEEEEEEECCCCCCCEEEHHHHHHHHHCCEEEECCCCCCHHHHHHHCCCHHHHHHCCH
TLEELVETMVEAVEDSRTVVRLVSGDPFVYSSLYEQVRELRRRGVDYEVIPGVSSVNAAA
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHH
AALGEELTKPGISQTVILTRPAGRTGKPEGESLSELAEHGCTMVIFLGAAYLERIVKSLL
HHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHH
GGAYDEDTPAAVVYKASTPEEKVILGTLGDIAEKAREEGIDRTALIIVGDVLKEEGKRSH
CCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCHHC
LYSEEYARRVRR
CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]