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The map label for this gene is CobJ [H]

Identifier: 20094812

GI number: 20094812

Start: 1403490

End: 1404254

Strand: Direct

Name: CobJ [H]

Synonym: MK1376

Alternate gene names: 20094812

Gene position: 1403490-1404254 (Clockwise)

Preceding gene: 20094811

Following gene: 20094817

Centisome position: 82.8

GC content: 66.27

Gene sequence:

>765_bases
ATGATCGTGGGCAAGCTGTACGTGGTCGGAACGGGACCCGGAGACCCGGATCTGATGACGGTTAAGGCCCAGAGAGTGCT
ACGGCACGTGGACGTGGTGGTGGGGTACCGGACGTACGTCGACCTCATCGAGGAGATCCTACCGGAAGACGTCGAGATAA
AGCGGTACGGCATGCGGGAAGAACTCGACCGGGCGCGGGAGGCCGTACGGCTGGCGGCCGATGGGTTCGAGGTGGCCCTC
GTCAGTGGTGGAGACCCCGGCGTCTACGGGATGGCCGGCGTCGTCCTCCCGATCGCGGTCGAGGAGGGCGTCGAGGTCGA
GGTCGTACCCGGGGTCACGGCGGCCTGCGCGGCCTCCGCGCTGCTCGGTGCGCCGCTCATGCTGGACTTCGCGGCCGTGA
GCTTGAGCGACCACCTCGTACCGCTGGAGGAGATACTGGAGAGGGTCCGGGCGGCCCTCGAGGCCGACTTCGTCCTGGTC
GTGTACAACCCGAACAGTTCCGAGCGTAGGCACATCTTCGAAGCCTTCGTCGACGTCCTCGAGGAGATAGTGGAAGAGGA
CAGGCCGGTGGGGATAGTCCGGAACGCCTACCGCGAGAGGCAGAGCGTCGAGGTAGTGCGGGTTCGCGAGCTGCGCGACC
TGGCCGACAGGATCGATATGCGGTCCATCCTGATCGTGGGGAGCTCGCGGACCCGGATGGTGGGCGATTGGTTGGTGACG
GAGCGTGGTTACTCCTCGCGCACCGGCCGGAACTCCAGCGAGTAG

Upstream 100 bases:

>100_bases
ATCCGGTACCTGAAGCTGCTGGGGGTTAAGGCGGGGAGCAGAGAAGAGGCGGGGCTCGTGGTAGACCCCGAGCGCGGTGA
GATCGTGGAGTTGCGGGGTG

Downstream 100 bases:

>100_bases
TAGATCACACCGTCCTCACCGTCCTCACTGATACGGCGGAACACGGCCTCCACCGGCGTGCCTACGTCGATCTCGCCGGG
CTCGCAGTCCACGATCATCG

Product: precorrin-3B methylase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3B C(17)-methyltransferase; Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase; Cobalt-precorrin-8X methylmutase; Cobalt-precorrin isomerase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MIVGKLYVVGTGPGDPDLMTVKAQRVLRHVDVVVGYRTYVDLIEEILPEDVEIKRYGMREELDRAREAVRLAADGFEVAL
VSGGDPGVYGMAGVVLPIAVEEGVEVEVVPGVTAACAASALLGAPLMLDFAAVSLSDHLVPLEEILERVRAALEADFVLV
VYNPNSSERRHIFEAFVDVLEEIVEEDRPVGIVRNAYRERQSVEVVRVRELRDLADRIDMRSILIVGSSRTRMVGDWLVT
ERGYSSRTGRNSSE

Sequences:

>Translated_254_residues
MIVGKLYVVGTGPGDPDLMTVKAQRVLRHVDVVVGYRTYVDLIEEILPEDVEIKRYGMREELDRAREAVRLAADGFEVAL
VSGGDPGVYGMAGVVLPIAVEEGVEVEVVPGVTAACAASALLGAPLMLDFAAVSLSDHLVPLEEILERVRAALEADFVLV
VYNPNSSERRHIFEAFVDVLEEIVEEDRPVGIVRNAYRERQSVEVVRVRELRDLADRIDMRSILIVGSSRTRMVGDWLVT
ERGYSSRTGRNSSE
>Mature_254_residues
MIVGKLYVVGTGPGDPDLMTVKAQRVLRHVDVVVGYRTYVDLIEEILPEDVEIKRYGMREELDRAREAVRLAADGFEVAL
VSGGDPGVYGMAGVVLPIAVEEGVEVEVVPGVTAACAASALLGAPLMLDFAAVSLSDHLVPLEEILERVRAALEADFVLV
VYNPNSSERRHIFEAFVDVLEEIVEEDRPVGIVRNAYRERQSVEVVRVRELRDLADRIDMRSILIVGSSRTRMVGDWLVT
ERGYSSRTGRNSSE

Specific function: Bifunctional enzyme with a methyltransferase domain that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 and an isomerase domain that catalyzes the conversion of cobalt-precorrin-8X to cobyrinic acid; a methyl migration

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the CobH family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=236, Percent_Identity=29.6610169491525, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014422
- InterPro:   IPR003722
- InterPro:   IPR006363 [H]

Pfam domain/function: PF02570 CbiC; PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 28020; Mature: 28020

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVGKLYVVGTGPGDPDLMTVKAQRVLRHVDVVVGYRTYVDLIEEILPEDVEIKRYGMRE
CEEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHH
ELDRAREAVRLAADGFEVALVSGGDPGVYGMAGVVLPIAVEEGVEVEVVPGVTAACAASA
HHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHEEEEECCCCEEEEECCCHHHHHHHH
LLGAPLMLDFAAVSLSDHLVPLEEILERVRAALEADFVLVVYNPNSSERRHIFEAFVDVL
HHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHH
EEIVEEDRPVGIVRNAYRERQSVEVVRVRELRDLADRIDMRSILIVGSSRTRMVGDWLVT
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHEE
ERGYSSRTGRNSSE
CCCCCCCCCCCCCC
>Mature Secondary Structure
MIVGKLYVVGTGPGDPDLMTVKAQRVLRHVDVVVGYRTYVDLIEEILPEDVEIKRYGMRE
CEEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHH
ELDRAREAVRLAADGFEVALVSGGDPGVYGMAGVVLPIAVEEGVEVEVVPGVTAACAASA
HHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHEEEEECCCCEEEEECCCHHHHHHHH
LLGAPLMLDFAAVSLSDHLVPLEEILERVRAALEADFVLVVYNPNSSERRHIFEAFVDVL
HHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHH
EEIVEEDRPVGIVRNAYRERQSVEVVRVRELRDLADRIDMRSILIVGSSRTRMVGDWLVT
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHEE
ERGYSSRTGRNSSE
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]