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The map label for this gene is Nth_2 [C]

Identifier: 20094798

GI number: 20094798

Start: 1385837

End: 1386457

Strand: Reverse

Name: Nth_2 [C]

Synonym: MK1362

Alternate gene names: 20094798

Gene position: 1386457-1385837 (Counterclockwise)

Preceding gene: 20094801

Following gene: 20094795

Centisome position: 81.8

GC content: 69.73

Gene sequence:

>621_bases
GTGGCGACGCTCTTGGAGGCGCCGAGAATCGTCGAGCGGTGGCTCCGGGGGATGGAGGAGCGCGGGGAGTACGAACCGGC
CTGGGGCGATAGGGAAGACGTCTACTCGGTCGCGCTCGCGGGTGTGTTACATCAACGTACCCGGAGGGAGCTAGCCGAGC
CGGTGCTCAGGGAACTGCTGCGCCGGTACCCGGAACCCTCGGACCTCCTGAAGGCCCCGGAGGACGAGCTGAAGGAGTCG
CTCGCGCGGATCGGTCTGGTGGAACGGAGGCTGAAGGCCGTCCTGGGGCTGGCCAGGCTGCTGTCGGAAGACCCGGAACC
CTCGGGCGAGGATCTGCTCTCGGTGCCCGGGGTGGGCCCGTACACCGCGGACCTGGTGCGGGCGGTCGTGTACCGGGAGC
GGGTCCTGCCCGTGGACGCGAACGTGCGTCGCGTGGTGAGACGGTCGACTGGGCGTCCCGTGGGAGATGTCGGCGCGGAG
TGGGTGAGAGCCGCCCGGGATCCCAGGGACCTCGCGCTGGGTACTGTGGAACTGGGACGGAGGTGCTGCCGTCCGGAGCC
GGAGTGTGAGGAGTGTCCGATCGCGGGCGTTTGCGCGGAGCAGGGAAGAGTACGTGGGTGA

Upstream 100 bases:

>100_bases
CGGTGGGTCGGTGCCCCTGGGTGTCCTCACCGGTTCGGCGATCCCCTGCCTCGTCATCCCGGAACCCTCATCCGGGTCTG
GGCACGGAAAACCGTTCCGG

Downstream 100 bases:

>100_bases
GAGCGCTTCAGACGGTTAGCCCGGCGCGGGGATCTCGAACCGGTCGACGCGCTCCCCGGTGGTGGCATCGTAGGCCTCGA
CGACGATCCGGTCGAACCGC

Product: A/G-specific DNA glycosylase

Products: NA

Alternate protein names: Mismatch glycosylase; MIG; Thymine-DNA glycosylase [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MATLLEAPRIVERWLRGMEERGEYEPAWGDREDVYSVALAGVLHQRTRRELAEPVLRELLRRYPEPSDLLKAPEDELKES
LARIGLVERRLKAVLGLARLLSEDPEPSGEDLLSVPGVGPYTADLVRAVVYRERVLPVDANVRRVVRRSTGRPVGDVGAE
WVRAARDPRDLALGTVELGRRCCRPEPECEECPIAGVCAEQGRVRG

Sequences:

>Translated_206_residues
MATLLEAPRIVERWLRGMEERGEYEPAWGDREDVYSVALAGVLHQRTRRELAEPVLRELLRRYPEPSDLLKAPEDELKES
LARIGLVERRLKAVLGLARLLSEDPEPSGEDLLSVPGVGPYTADLVRAVVYRERVLPVDANVRRVVRRSTGRPVGDVGAE
WVRAARDPRDLALGTVELGRRCCRPEPECEECPIAGVCAEQGRVRG
>Mature_205_residues
ATLLEAPRIVERWLRGMEERGEYEPAWGDREDVYSVALAGVLHQRTRRELAEPVLRELLRRYPEPSDLLKAPEDELKESL
ARIGLVERRLKAVLGLARLLSEDPEPSGEDLLSVPGVGPYTADLVRAVVYRERVLPVDANVRRVVRRSTGRPVGDVGAEW
VRAARDPRDLALGTVELGRRCCRPEPECEECPIAGVCAEQGRVRG

Specific function: Could act in DNA repair. Restriction methylase M.mthtI, which is encoded by this plasmid, generates 5-methylcytosine which is, especially under thermophilic conditions, subject to deamination resulting in G-T mismatches. This protein could correct these m

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 23079; Mature: 22948

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLLEAPRIVERWLRGMEERGEYEPAWGDREDVYSVALAGVLHQRTRRELAEPVLRELL
CCCHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRYPEPSDLLKAPEDELKESLARIGLVERRLKAVLGLARLLSEDPEPSGEDLLSVPGVGP
HHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCC
YTADLVRAVVYRERVLPVDANVRRVVRRSTGRPVGDVGAEWVRAARDPRDLALGTVELGR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
RCCRPEPECEECPIAGVCAEQGRVRG
HHCCCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
ATLLEAPRIVERWLRGMEERGEYEPAWGDREDVYSVALAGVLHQRTRRELAEPVLRELL
CCHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRYPEPSDLLKAPEDELKESLARIGLVERRLKAVLGLARLLSEDPEPSGEDLLSVPGVGP
HHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCC
YTADLVRAVVYRERVLPVDANVRRVVRRSTGRPVGDVGAEWVRAARDPRDLALGTVELGR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
RCCRPEPECEECPIAGVCAEQGRVRG
HHCCCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1336177; 11786018 [H]