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The map label for this gene is HisB

Identifier: 20094291

GI number: 20094291

Start: 812269

End: 812874

Strand: Reverse

Name: HisB

Synonym: MK0855

Alternate gene names: 20094291

Gene position: 812874-812269 (Counterclockwise)

Preceding gene: 20094294

Following gene: 20094290

Centisome position: 47.96

GC content: 56.93

Gene sequence:

>606_bases
GTGAACTCGGTGCGGACGGCGGATGTCAAGCGTACGACGAAGGAAACGGAAGTTGAGGTGAGCGTGAACCTAGACGGATC
CGGTCGCGCTAAGGTCGACACCGGTCTTCCGTTTTTCGACCACCTCCTGCACCAGTTCGCCTTTCACGGACGTATAGATG
TTGAGATCAAAGCCCGAGGCGACCTAGAGGTCGACGATCATCACACCGTCGAGGACGTCGGTATATGCCTGGGTAAAGCC
TTGAACGAAGCACTCGGAGATCGCGAGGGTATCCGACGGATCGCATGGGCACTGGTACCTATGGATGAAGCGCTAGTAGA
ATGTGCTGTAGATATCTCCGGAAGGCCTTACTTCGTCTTAAAGGGGTACCGACCCCGACGCAATAGAATCGGTTCTCCTC
CATTATCCACGGAGAACGTCTCGCACTTCTGGAAAAGCTTCTGCGACCACGCCGGCGTAACCATGCATGTGGTGGTACGG
TGGTGGGACAACGACCATCACGCCATCGAAGCCATGTTTAAGGCCGTAGGCCGCGCACTCGGCGCAGCGAAGGAAATCGT
CGGTGACGGAGTGCCATCCACTAAGGGAACGCTCCGACGCGGGTGA

Upstream 100 bases:

>100_bases
TAGGACGATGCAGACGATGGAAACCGTCCTCTCCATGGGGACCCTAGATCGGGCCGCACGTCCGTGCTATTTACCGCCAC
CAACCGACGTTTGCGATGGG

Downstream 100 bases:

>100_bases
GCGGATGAAAGCTTTCAGTCTCGTAGAACCGTCCCCGGCCAAGAACCTCCTCCCGGTGTGGGAGCGACTCCTGGAGGAAG
GGTGGGAAGTAACAGTATAC

Product: imidazoleglycerol-phosphate dehydratase

Products: NA

Alternate protein names: IGPD

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MNSVRTADVKRTTKETEVEVSVNLDGSGRAKVDTGLPFFDHLLHQFAFHGRIDVEIKARGDLEVDDHHTVEDVGICLGKA
LNEALGDREGIRRIAWALVPMDEALVECAVDISGRPYFVLKGYRPRRNRIGSPPLSTENVSHFWKSFCDHAGVTMHVVVR
WWDNDHHAIEAMFKAVGRALGAAKEIVGDGVPSTKGTLRRG

Sequences:

>Translated_201_residues
MNSVRTADVKRTTKETEVEVSVNLDGSGRAKVDTGLPFFDHLLHQFAFHGRIDVEIKARGDLEVDDHHTVEDVGICLGKA
LNEALGDREGIRRIAWALVPMDEALVECAVDISGRPYFVLKGYRPRRNRIGSPPLSTENVSHFWKSFCDHAGVTMHVVVR
WWDNDHHAIEAMFKAVGRALGAAKEIVGDGVPSTKGTLRRG
>Mature_201_residues
MNSVRTADVKRTTKETEVEVSVNLDGSGRAKVDTGLPFFDHLLHQFAFHGRIDVEIKARGDLEVDDHHTVEDVGICLGKA
LNEALGDREGIRRIAWALVPMDEALVECAVDISGRPYFVLKGYRPRRNRIGSPPLSTENVSHFWKSFCDHAGVTMHVVVR
WWDNDHHAIEAMFKAVGRALGAAKEIVGDGVPSTKGTLRRG

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the imidazoleglycerol-phosphate dehydratase family

Homologues:

Organism=Escherichia coli, GI87082027, Length=194, Percent_Identity=50, Blast_Score=181, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324776, Length=219, Percent_Identity=37.4429223744292, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS7_METKA (P58879)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614138.1
- ProteinModelPortal:   P58879
- SMR:   P58879
- GeneID:   1476956
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0855
- NMPDR:   fig|190192.1.peg.851
- HOGENOM:   HBG289010
- OMA:   TLHVETL
- BioCyc:   MKAN190192:MK0855-MONOMER
- BRENDA:   4.2.1.19
- GO:   GO:0005737
- HAMAP:   MF_00076
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR020568

Pfam domain/function: PF00475 IGPD; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =4.2.1.19

Molecular weight: Translated: 22293; Mature: 22293

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00954 IGP_DEHYDRATASE_1; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVRTADVKRTTKETEVEVSVNLDGSGRAKVDTGLPFFDHLLHQFAFHGRIDVEIKARG
CCCCCCCHHHHCCCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCEEEEEEEECC
DLEVDDHHTVEDVGICLGKALNEALGDREGIRRIAWALVPMDEALVECAVDISGRPYFVL
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEE
KGYRPRRNRIGSPPLSTENVSHFWKSFCDHAGVTMHVVVRWWDNDHHAIEAMFKAVGRAL
ECCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHH
GAAKEIVGDGVPSTKGTLRRG
HHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSVRTADVKRTTKETEVEVSVNLDGSGRAKVDTGLPFFDHLLHQFAFHGRIDVEIKARG
CCCCCCCHHHHCCCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCEEEEEEEECC
DLEVDDHHTVEDVGICLGKALNEALGDREGIRRIAWALVPMDEALVECAVDISGRPYFVL
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEE
KGYRPRRNRIGSPPLSTENVSHFWKSFCDHAGVTMHVVVRWWDNDHHAIEAMFKAVGRAL
ECCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHH
GAAKEIVGDGVPSTKGTLRRG
HHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014