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The map label for this gene is SUA7_2

Identifier: 20094294

GI number: 20094294

Start: 814061

End: 814984

Strand: Reverse

Name: SUA7_2

Synonym: MK0858

Alternate gene names: 20094294

Gene position: 814984-814061 (Counterclockwise)

Preceding gene: 20094295

Following gene: 20094291

Centisome position: 48.08

GC content: 58.01

Gene sequence:

>924_bases
ATGGCTGCTTCTGAAAGGAACACCGGGAAGTTCACGGAGGAATGTCCGGCGTGTGGTTCCGCAGAGATTGTTTTCGATGA
GGAGCGGGGTGAGTACGTCTGCGCGAATTGCGGACTGGTCACGGAAGACCCGGTGATAGATCCCGGCCCTGAGTGGCGGC
ACTTTAACCCGGACCAGAGACAACGGAGGTCTCGAACTGGAGAGCCCGTCAAGCTGAGGTTGCCGGATAAGGGCATCTCC
ACGATTATAGACCGGGAGTTGCGCGACTCCGGTGGGAAAAAGAACCCCCGGATGCGGCGCATTCGCACCTGGGATGCACG
TATCAAAGTATCGGGAAGTCGGGAAAGGAACTTTTTCCAGGCGTTCCTGGAGCTGGAGAATCTCGCGTCCAAGCTTCAGT
TACCCGAGTCGGTCAGGGAACTCGCCGCCTCGATATACAGGAAGGCGTACAAAGAAGGGATCGTGCGTGGCCGCGGCATA
GAGAGCGTTTTGGGAGCGGCGGTATTCGCCGCGTGTAAAGAAGCTCGCGTGCCCCGGACTGCCCGCGAGATCGCGGAGGC
GCTCGGAGTCTCGGATGAGAACGAGATACTCCGGGCTTATCGAGTACTGCAACGTCGGCTGAACCTGAAGCAGAAACCGA
CTGAACCCTCCGATCACCTACCCAGGTTCGCCTCGAAGTTAGGGGTCTCCGAAAACGTCCAGGCTAAGGCTCAAGAGATC
ATCGAAAAGGCCAAGGAGAAAGGCATCACCGTGGGTAAGGGGCCAGCCGGCGTAGCCGCGGCCGCATTATACATCGCGTC
CATCCTCGAGGGAGAGAGACGTACTCAGAAGGAGATTGCGGAGGTTGCTCGAGTTACGGAAGTTACGATCCGAAACCGGT
ACAAGGAGATCTGTGAAGCCCTCGGGATCGAGCTTCATCCATGA

Upstream 100 bases:

>100_bases
CGGGAAGGAGCTCTTCATACCACCCAGACGACGGCGAAGGCGTCCTCCTCGCAGGCGGCGGGGTCGGCCCCGCCGCCGCA
GATGATGGGGTGTATCGAGA

Downstream 100 bases:

>100_bases
CGAAGCAGCTCACGCGCCGCCTTATCCACGATCTTTTCTTCCGACTCGGGGATGGGGTAAGCCCTCGCATCCAACACGTA
CCGTATTCTTTCATCGACTA

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MAASERNTGKFTEECPACGSAEIVFDEERGEYVCANCGLVTEDPVIDPGPEWRHFNPDQRQRRSRTGEPVKLRLPDKGIS
TIIDRELRDSGGKKNPRMRRIRTWDARIKVSGSRERNFFQAFLELENLASKLQLPESVRELAASIYRKAYKEGIVRGRGI
ESVLGAAVFAACKEARVPRTAREIAEALGVSDENEILRAYRVLQRRLNLKQKPTEPSDHLPRFASKLGVSENVQAKAQEI
IEKAKEKGITVGKGPAGVAAAALYIASILEGERRTQKEIAEVARVTEVTIRNRYKEICEALGIELHP

Sequences:

>Translated_307_residues
MAASERNTGKFTEECPACGSAEIVFDEERGEYVCANCGLVTEDPVIDPGPEWRHFNPDQRQRRSRTGEPVKLRLPDKGIS
TIIDRELRDSGGKKNPRMRRIRTWDARIKVSGSRERNFFQAFLELENLASKLQLPESVRELAASIYRKAYKEGIVRGRGI
ESVLGAAVFAACKEARVPRTAREIAEALGVSDENEILRAYRVLQRRLNLKQKPTEPSDHLPRFASKLGVSENVQAKAQEI
IEKAKEKGITVGKGPAGVAAAALYIASILEGERRTQKEIAEVARVTEVTIRNRYKEICEALGIELHP
>Mature_306_residues
AASERNTGKFTEECPACGSAEIVFDEERGEYVCANCGLVTEDPVIDPGPEWRHFNPDQRQRRSRTGEPVKLRLPDKGIST
IIDRELRDSGGKKNPRMRRIRTWDARIKVSGSRERNFFQAFLELENLASKLQLPESVRELAASIYRKAYKEGIVRGRGIE
SVLGAAVFAACKEARVPRTAREIAEALGVSDENEILRAYRVLQRRLNLKQKPTEPSDHLPRFASKLGVSENVQAKAQEII
EKAKEKGITVGKGPAGVAAAALYIASILEGERRTQKEIAEVARVTEVTIRNRYKEICEALGIELHP

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger

Homologues:

Organism=Homo sapiens, GI4504193, Length=302, Percent_Identity=32.4503311258278, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI22035556, Length=289, Percent_Identity=25.6055363321799, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17564892, Length=295, Percent_Identity=29.8305084745763, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17531397, Length=291, Percent_Identity=23.0240549828179, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325343, Length=303, Percent_Identity=28.0528052805281, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321685, Length=189, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17136758, Length=286, Percent_Identity=32.5174825174825, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI281361961, Length=289, Percent_Identity=24.9134948096886, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24647658, Length=289, Percent_Identity=24.9134948096886, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B_METKA (Q8TX21)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614141.1
- ProteinModelPortal:   Q8TX21
- GeneID:   1476959
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0858
- NMPDR:   fig|190192.1.peg.854
- HOGENOM:   HBG646536
- OMA:   HDYERAE
- BioCyc:   MKAN190192:MK0858-MONOMER
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00685
- SMART:   SM00385

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 34320; Mature: 34188

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS00782 TFIIB; PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASERNTGKFTEECPACGSAEIVFDEERGEYVCANCGLVTEDPVIDPGPEWRHFNPDQR
CCCCCCCCCCCHHHCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHH
QRRSRTGEPVKLRLPDKGISTIIDRELRDSGGKKNPRMRRIRTWDARIKVSGSRERNFFQ
HHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEEEECCCHHHHHHH
AFLELENLASKLQLPESVRELAASIYRKAYKEGIVRGRGIESVLGAAVFAACKEARVPRT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHH
AREIAEALGVSDENEILRAYRVLQRRLNLKQKPTEPSDHLPRFASKLGVSENVQAKAQEI
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
IEKAKEKGITVGKGPAGVAAAALYIASILEGERRTQKEIAEVARVTEVTIRNRYKEICEA
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIELHP
HCCCCCC
>Mature Secondary Structure 
AASERNTGKFTEECPACGSAEIVFDEERGEYVCANCGLVTEDPVIDPGPEWRHFNPDQR
CCCCCCCCCCHHHCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHH
QRRSRTGEPVKLRLPDKGISTIIDRELRDSGGKKNPRMRRIRTWDARIKVSGSRERNFFQ
HHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEEEECCCHHHHHHH
AFLELENLASKLQLPESVRELAASIYRKAYKEGIVRGRGIESVLGAAVFAACKEARVPRT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHH
AREIAEALGVSDENEILRAYRVLQRRLNLKQKPTEPSDHLPRFASKLGVSENVQAKAQEI
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
IEKAKEKGITVGKGPAGVAAAALYIASILEGERRTQKEIAEVARVTEVTIRNRYKEICEA
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIELHP
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014