Click here to switch to the map view.

The map label for this gene is nirB [C]

Identifier: 20094236

GI number: 20094236

Start: 760363

End: 762369

Strand: Reverse

Name: nirB [C]

Synonym: MK0799

Alternate gene names: 20094236

Gene position: 762369-760363 (Counterclockwise)

Preceding gene: 20094239

Following gene: 20094229

Centisome position: 44.98

GC content: 59.24

Gene sequence:

>2007_bases
GTGCGGAGTATCGTCACATCAGAGCACGGGGGACACTACTTGACAGAAGAATTCGAGTGGAAACTTACCGAGGAAGTCGT
CGAGCCCGGGGCCTGTGCACTATGCGGCACTTGTGTCGCGATCTGCCCGGGCGGGATAATCGAGCTCACGGACGAGGGCC
CGCGACTCACTGAGGAGTGCGCCCGGAAGGGAACAGGTAACTGTCACACTGTGTGCCCGCGTGTCGACACCGCAGCCTAC
CATCTCGGGCTACGTATCGCAGGCCGCGACTACGAACCTCTCACAGGCGGTTACCGGCGTGCAGTAGGGGCTGTCGCCGC
CGATTCCGACCTCCGAGAACTCGGTCAAGATGGAGGCGCCGTTACGGCACTAGCCCGATACGCCCTCGAAGAAGGTCTCG
CCGATGCCGTCGTCGGAGTGACCGCCGGCTCCGCTTGGAAGCCGTGTGTTACCGTCGTAGAAGATCCCGAAAAGGTGAAG
GACCTGGCCGGATCCAAGTACACACGCGTTGGACTGGTTGAGGCTCTGGCTGAAGCCGCGGATCGTGGTATTGAGCGTGT
GCTCGCGATAGGTCTCCCCTGCCAGGTGAATGGGCTCGCAAAGATTCAGCACTTCGAGATAGTAGCTAAAGGCGCACGGG
CCTTGCGCAACATCGACGGGAGTCCGGCCGAGAAGCTCCCGGAAGTCGTAGCCACTATCGGACTATTCTGTACCAAAAAC
TTCGAGTACGAAGGCCTTGTGAAGCTCCTGAGAGAGAAAGGTGTCGATATCGAGGACGTGGAGCGATTCGATATCACTTC
AGGAAAGCTCCGCGTCGAGATATCAGGCGGTGAAACTAAGGAGTACGATGTGAAGGAGTTTGAAGAGGCGATCCCCGAAG
GCTGCCGTATCTGTAACGACTTCACCGCCAGACTAGCCGACGTCTCCGTCGGCTCCGTAGGAACTCCCGAAGGAGTTACC
ACCCTCCTCATCCGCAGCGAGACCGGTGAAGAACTCGTTGAAGGTGCTGTCGAGGCCGGGTATCTACGGCTTCGAGATGC
CGTTAACGGGTCGGATGTGCGAAGGCTCGCAAAGCTCAAGTGCGACTGGGCCATGAAAGAGGCGAAGAATCGGCTGAAAG
AGGGGCGGAAAGTTCCACCGTTTTGGGTCGGAGACTACGGAGGAGTCATCACCCGGGCGGACGGTACCTTTGCGGTCAGG
CTAAAGGTCGGTCCAGGTGGCTGGGTCGACGATCCCGACATCTTCCGGGCGATTTCGGGGTTCCTCGAAGAAGGTTACAG
GGCCAAGTTCACCGACCGGCAGCAGCTCGAGATCCATGGTATCCCAGCTACAGAGATACCCGAAGTCGTGGAACGGCTCC
GAGAGACCGGACTGAACACGGGGTCCGAGGGTCCCCTCGTCCGAACGATCATGGCATGCCCGGGGAAGGACAACTGCTCT
TCGGGCATTTTGAACACCGAAGAGCTCGCCAGAAAGCTAGAGGAAGAACTAGCGGAAGAGCCCACCCCGTATAAGTTCAA
GATCGCGCTATCCGGATGCACGAACAGCTGCGTTCGACCTCAACACCATGACCTCGGTTTCGCCGGCGCCGTGAGGCCCG
GAGTGGACCCGGAGAAGTGCACTGGATGCGGTCAGTGTGTGGATGCCTGTAAGGTCGACGCGATCCGAATCATCACTGTG
GGAGGACAGGCGGCGGTGGCGGACACCGATTACAAGCGTTGCGTCTACTGTGGCAAGTGTATCAACGTCTGTCCGGAGGA
AGCCAGGTACGCCGAGAAGGAGGGCATCATCGTCTGGATCGGGGGTAAAGGAGGGCGAGAACCCGTCGAAGGAGCCAGGT
TGGACGTTTTCGCCGACCCTGACAGCATACCTACGATCGCTCACACCGTGATCGAAACGTATCGGGAACTGGCGGAAGAA
CCGCAGAAAGAGCGGTTGGCCGACACGATCCGCAAACACGGACTTCGACAGTTCACAGAGGCGATCCGGCGGGTCCTCTC
CCAATAA

Upstream 100 bases:

>100_bases
GAGCCTTCGGACGGTGACGAAGGGAACGTCTGCGAGCATCGGCACCCCCACCGGTCTAAGCCTAATCGGAATGTATATAT
AGATCCAGCCTCGTGGATGT

Downstream 100 bases:

>100_bases
ACACTTTTTTAAAGTCCAGTTGAAACCCGGAGTCCGACGTGTGCCTATCATCGAGCAACCCGCACTTCCAGTCCCAGGGC
GCGTAGCTTTCGGAGTAATC

Product: bifunctional coenzyme F420-reducing hydrogenase subunit beta/oxidoreductase

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MRSIVTSEHGGHYLTEEFEWKLTEEVVEPGACALCGTCVAICPGGIIELTDEGPRLTEECARKGTGNCHTVCPRVDTAAY
HLGLRIAGRDYEPLTGGYRRAVGAVAADSDLRELGQDGGAVTALARYALEEGLADAVVGVTAGSAWKPCVTVVEDPEKVK
DLAGSKYTRVGLVEALAEAADRGIERVLAIGLPCQVNGLAKIQHFEIVAKGARALRNIDGSPAEKLPEVVATIGLFCTKN
FEYEGLVKLLREKGVDIEDVERFDITSGKLRVEISGGETKEYDVKEFEEAIPEGCRICNDFTARLADVSVGSVGTPEGVT
TLLIRSETGEELVEGAVEAGYLRLRDAVNGSDVRRLAKLKCDWAMKEAKNRLKEGRKVPPFWVGDYGGVITRADGTFAVR
LKVGPGGWVDDPDIFRAISGFLEEGYRAKFTDRQQLEIHGIPATEIPEVVERLRETGLNTGSEGPLVRTIMACPGKDNCS
SGILNTEELARKLEEELAEEPTPYKFKIALSGCTNSCVRPQHHDLGFAGAVRPGVDPEKCTGCGQCVDACKVDAIRIITV
GGQAAVADTDYKRCVYCGKCINVCPEEARYAEKEGIIVWIGGKGGREPVEGARLDVFADPDSIPTIAHTVIETYRELAEE
PQKERLADTIRKHGLRQFTEAIRRVLSQ

Sequences:

>Translated_668_residues
MRSIVTSEHGGHYLTEEFEWKLTEEVVEPGACALCGTCVAICPGGIIELTDEGPRLTEECARKGTGNCHTVCPRVDTAAY
HLGLRIAGRDYEPLTGGYRRAVGAVAADSDLRELGQDGGAVTALARYALEEGLADAVVGVTAGSAWKPCVTVVEDPEKVK
DLAGSKYTRVGLVEALAEAADRGIERVLAIGLPCQVNGLAKIQHFEIVAKGARALRNIDGSPAEKLPEVVATIGLFCTKN
FEYEGLVKLLREKGVDIEDVERFDITSGKLRVEISGGETKEYDVKEFEEAIPEGCRICNDFTARLADVSVGSVGTPEGVT
TLLIRSETGEELVEGAVEAGYLRLRDAVNGSDVRRLAKLKCDWAMKEAKNRLKEGRKVPPFWVGDYGGVITRADGTFAVR
LKVGPGGWVDDPDIFRAISGFLEEGYRAKFTDRQQLEIHGIPATEIPEVVERLRETGLNTGSEGPLVRTIMACPGKDNCS
SGILNTEELARKLEEELAEEPTPYKFKIALSGCTNSCVRPQHHDLGFAGAVRPGVDPEKCTGCGQCVDACKVDAIRIITV
GGQAAVADTDYKRCVYCGKCINVCPEEARYAEKEGIIVWIGGKGGREPVEGARLDVFADPDSIPTIAHTVIETYRELAEE
PQKERLADTIRKHGLRQFTEAIRRVLSQ
>Mature_668_residues
MRSIVTSEHGGHYLTEEFEWKLTEEVVEPGACALCGTCVAICPGGIIELTDEGPRLTEECARKGTGNCHTVCPRVDTAAY
HLGLRIAGRDYEPLTGGYRRAVGAVAADSDLRELGQDGGAVTALARYALEEGLADAVVGVTAGSAWKPCVTVVEDPEKVK
DLAGSKYTRVGLVEALAEAADRGIERVLAIGLPCQVNGLAKIQHFEIVAKGARALRNIDGSPAEKLPEVVATIGLFCTKN
FEYEGLVKLLREKGVDIEDVERFDITSGKLRVEISGGETKEYDVKEFEEAIPEGCRICNDFTARLADVSVGSVGTPEGVT
TLLIRSETGEELVEGAVEAGYLRLRDAVNGSDVRRLAKLKCDWAMKEAKNRLKEGRKVPPFWVGDYGGVITRADGTFAVR
LKVGPGGWVDDPDIFRAISGFLEEGYRAKFTDRQQLEIHGIPATEIPEVVERLRETGLNTGSEGPLVRTIMACPGKDNCS
SGILNTEELARKLEEELAEEPTPYKFKIALSGCTNSCVRPQHHDLGFAGAVRPGVDPEKCTGCGQCVDACKVDAIRIITV
GGQAAVADTDYKRCVYCGKCINVCPEEARYAEKEGIIVWIGGKGGREPVEGARLDVFADPDSIPTIAHTVIETYRELAEE
PQKERLADTIRKHGLRQFTEAIRRVLSQ

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1035

COG function: function code C; Coenzyme F420-reducing hydrogenase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR007516
- InterPro:   IPR007525
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF00037 Fer4; PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 72336; Mature: 72336

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00365 NIR_SIR ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIVTSEHGGHYLTEEFEWKLTEEVVEPGACALCGTCVAICPGGIIELTDEGPRLTEEC
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHH
ARKGTGNCHTVCPRVDTAAYHLGLRIAGRDYEPLTGGYRRAVGAVAADSDLRELGQDGGA
HHCCCCCCEEECCCCCHHHHEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCH
VTALARYALEEGLADAVVGVTAGSAWKPCVTVVEDPEKVKDLAGSKYTRVGLVEALAEAA
HHHHHHHHHHHCHHHHHHHCCCCCCCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHH
DRGIERVLAIGLPCQVNGLAKIQHFEIVAKGARALRNIDGSPAEKLPEVVATIGLFCTKN
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
FEYEGLVKLLREKGVDIEDVERFDITSGKLRVEISGGETKEYDVKEFEEAIPEGCRICND
CCHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
FTARLADVSVGSVGTPEGVTTLLIRSETGEELVEGAVEAGYLRLRDAVNGSDVRRLAKLK
HHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CDWAMKEAKNRLKEGRKVPPFWVGDYGGVITRADGTFAVRLKVGPGGWVDDPDIFRAISG
HHHHHHHHHHHHHCCCCCCCEEECCCCCEEEECCCEEEEEEEECCCCCCCCHHHHHHHHH
FLEEGYRAKFTDRQQLEIHGIPATEIPEVVERLRETGLNTGSEGPLVRTIMACPGKDNCS
HHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCC
SGILNTEELARKLEEELAEEPTPYKFKIALSGCTNSCVRPQHHDLGFAGAVRPGVDPEKC
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHH
TGCGQCVDACKVDAIRIITVGGQAAVADTDYKRCVYCGKCINVCPEEARYAEKEGIIVWI
CCHHHHHHHHCCCEEEEEEECCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCEEEEE
GGKGGREPVEGARLDVFADPDSIPTIAHTVIETYRELAEEPQKERLADTIRKHGLRQFTE
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
AIRRVLSQ
HHHHHHCC
>Mature Secondary Structure
MRSIVTSEHGGHYLTEEFEWKLTEEVVEPGACALCGTCVAICPGGIIELTDEGPRLTEEC
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHH
ARKGTGNCHTVCPRVDTAAYHLGLRIAGRDYEPLTGGYRRAVGAVAADSDLRELGQDGGA
HHCCCCCCEEECCCCCHHHHEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCH
VTALARYALEEGLADAVVGVTAGSAWKPCVTVVEDPEKVKDLAGSKYTRVGLVEALAEAA
HHHHHHHHHHHCHHHHHHHCCCCCCCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHH
DRGIERVLAIGLPCQVNGLAKIQHFEIVAKGARALRNIDGSPAEKLPEVVATIGLFCTKN
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
FEYEGLVKLLREKGVDIEDVERFDITSGKLRVEISGGETKEYDVKEFEEAIPEGCRICND
CCHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
FTARLADVSVGSVGTPEGVTTLLIRSETGEELVEGAVEAGYLRLRDAVNGSDVRRLAKLK
HHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CDWAMKEAKNRLKEGRKVPPFWVGDYGGVITRADGTFAVRLKVGPGGWVDDPDIFRAISG
HHHHHHHHHHHHHCCCCCCCEEECCCCCEEEECCCEEEEEEEECCCCCCCCHHHHHHHHH
FLEEGYRAKFTDRQQLEIHGIPATEIPEVVERLRETGLNTGSEGPLVRTIMACPGKDNCS
HHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCC
SGILNTEELARKLEEELAEEPTPYKFKIALSGCTNSCVRPQHHDLGFAGAVRPGVDPEKC
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHH
TGCGQCVDACKVDAIRIITVGGQAAVADTDYKRCVYCGKCINVCPEEARYAEKEGIIVWI
CCHHHHHHHHCCCEEEEEEECCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCEEEEE
GGKGGREPVEGARLDVFADPDSIPTIAHTVIETYRELAEEPQKERLADTIRKHGLRQFTE
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
AIRRVLSQ
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]