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The map label for this gene is CarB_1 [H]

Identifier: 20094191

GI number: 20094191

Start: 714964

End: 716103

Strand: Reverse

Name: CarB_1 [H]

Synonym: MK0754

Alternate gene names: 20094191

Gene position: 716103-714964 (Counterclockwise)

Preceding gene: 20094192

Following gene: 20094190

Centisome position: 42.25

GC content: 59.47

Gene sequence:

>1140_bases
GTGAAGGTACTCTTCGTCGGCGCGAGGCTACTCCGGGACGTGGCGGAGTACGCCGGCTCCACGGGAGTGTACCGTATACT
CACCGAGTCGAACCCGAGGAGCAGGGACTGGGAGCTGGCGGATGAGGTGCACTTCGTACCGCGCGGTATGGAACATCCCA
CGAGAATCGCCCGCGAGCGGCACGTAGACGGTGTAGTACCACTCATCGGTGTAGACGATCCGCTACCGGCAGTGGGGGAG
ATGAAAGAAGAGCTGGAAGCCGAAGGTATCCGGGTTGTGGCGTCTGATCGCGAGAGCGTTGAAATCGGGATCGATAAAGT
GAGAACGAAACGCGCCTTCGAAGAACTGAACGTACCGACGCCTGAATGGGAAGTCGTCGAGAACGAAAGGCCATCGATCA
GTCCACCGGTAGTCGTGAAGGATCCACGGTCACAGGCGGGGCTCGGAGTCACGGTCCACGAGACGCACAAGCCGAGAGTA
TCGGGACGTAAGCTGGTAGAGGAGTTCATCGAAGGTGCGGAAGTTTCGATCGAAGTTCTATCCTGGGACGGTGAGGTGGT
GCCGCTGGTTCCGGTGTTCAAAGGATCCACGGCGGACCGTCGTCATCCGATCGATCGTATGCGGATGAGCCCGGCCCCGA
TCGACCCGTCCGTGGAGCGCAAGATAAGAAGGTGCGCGGTAAGAGTCGTGGAACATCTGGGACTGGAGGGCAACGTGGAC
TTCGACGTGGTAGTCCGGGAGAACGAGTTCTGGTTCCTCGAGTTCAACCCGAGACCCAGCGGCACCAGGTACATGACGTC
CGGATGCACGGGAATATGGCCGCTCCGTGAGCTGGTCGATATGGCGGCCGATCGATGGAGGCCACCGAAGATAAGGAAAT
TATGGTGCGCCATCGAGTGTCCCGTTTGGCGCCCTACGGACAAGGATCCCCTGCGTGAGATGTTCGACGGCGGAGTGTAC
CACGTGTTCTACGAGTACTATGGACGCTTCGACGTAGGTGGTCGTGTCACCGTGAGGGGGGACTCTTTTAAAGAAGCCCT
CACGAGGCTCCGCTCAGCGCTCAAGGCTGCAGGAGCGGACGTGAAGCGGGCCGAACGTGAGTATCGACGGAGGCTGAGGG
AAATCGAGGAGTACTTATGA

Upstream 100 bases:

>100_bases
TCCGCTCGGACTGCGAAGTGGTAGTGTTGTTCGGAGAGGCTCCGCTGAAGTACCGGGAAGAGATCTCTGAAGAAGTCGAG
CGAGTGCTGGGGGACGAAAG

Downstream 100 bases:

>100_bases
CCGTGGGGAGCGGACGATGATCATCGAGACGTTTACACTCTCCACAGTAACCTCGGCCGTAACGGTTGGATTACTCCGCA
AGTACATGCGGGAAGCTAAC

Product: carbamoylphosphate synthase large subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKVLFVGARLLRDVAEYAGSTGVYRILTESNPRSRDWELADEVHFVPRGMEHPTRIARERHVDGVVPLIGVDDPLPAVGE
MKEELEAEGIRVVASDRESVEIGIDKVRTKRAFEELNVPTPEWEVVENERPSISPPVVVKDPRSQAGLGVTVHETHKPRV
SGRKLVEEFIEGAEVSIEVLSWDGEVVPLVPVFKGSTADRRHPIDRMRMSPAPIDPSVERKIRRCAVRVVEHLGLEGNVD
FDVVVRENEFWFLEFNPRPSGTRYMTSGCTGIWPLRELVDMAADRWRPPKIRKLWCAIECPVWRPTDKDPLREMFDGGVY
HVFYEYYGRFDVGGRVTVRGDSFKEALTRLRSALKAAGADVKRAEREYRRRLREIEEYL

Sequences:

>Translated_379_residues
MKVLFVGARLLRDVAEYAGSTGVYRILTESNPRSRDWELADEVHFVPRGMEHPTRIARERHVDGVVPLIGVDDPLPAVGE
MKEELEAEGIRVVASDRESVEIGIDKVRTKRAFEELNVPTPEWEVVENERPSISPPVVVKDPRSQAGLGVTVHETHKPRV
SGRKLVEEFIEGAEVSIEVLSWDGEVVPLVPVFKGSTADRRHPIDRMRMSPAPIDPSVERKIRRCAVRVVEHLGLEGNVD
FDVVVRENEFWFLEFNPRPSGTRYMTSGCTGIWPLRELVDMAADRWRPPKIRKLWCAIECPVWRPTDKDPLREMFDGGVY
HVFYEYYGRFDVGGRVTVRGDSFKEALTRLRSALKAAGADVKRAEREYRRRLREIEEYL
>Mature_379_residues
MKVLFVGARLLRDVAEYAGSTGVYRILTESNPRSRDWELADEVHFVPRGMEHPTRIARERHVDGVVPLIGVDDPLPAVGE
MKEELEAEGIRVVASDRESVEIGIDKVRTKRAFEELNVPTPEWEVVENERPSISPPVVVKDPRSQAGLGVTVHETHKPRV
SGRKLVEEFIEGAEVSIEVLSWDGEVVPLVPVFKGSTADRRHPIDRMRMSPAPIDPSVERKIRRCAVRVVEHLGLEGNVD
FDVVVRENEFWFLEFNPRPSGTRYMTSGCTGIWPLRELVDMAADRWRPPKIRKLWCAIECPVWRPTDKDPLREMFDGGVY
HVFYEYYGRFDVGGRVTVRGDSFKEALTRLRSALKAAGADVKRAEREYRRRLREIEEYL

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ATP-grasp domains [H]

Homologues:

None

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006275
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; PF02142 MGS [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 43362; Mature: 43362

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLFVGARLLRDVAEYAGSTGVYRILTESNPRSRDWELADEVHFVPRGMEHPTRIARER
CEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
HVDGVVPLIGVDDPLPAVGEMKEELEAEGIRVVASDRESVEIGIDKVRTKRAFEELNVPT
CCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCEEHHHHHHHHHHHHHHCCCCC
PEWEVVENERPSISPPVVVKDPRSQAGLGVTVHETHKPRVSGRKLVEEFIEGAEVSIEVL
CCCEEECCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEE
SWDGEVVPLVPVFKGSTADRRHPIDRMRMSPAPIDPSVERKIRRCAVRVVEHLGLEGNVD
ECCCCEEEEEEEECCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
FDVVVRENEFWFLEFNPRPSGTRYMTSGCTGIWPLRELVDMAADRWRPPKIRKLWCAIEC
EEEEEECCEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEEEC
PVWRPTDKDPLREMFDGGVYHVFYEYYGRFDVGGRVTVRGDSFKEALTRLRSALKAAGAD
CCCCCCCHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCH
VKRAEREYRRRLREIEEYL
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVLFVGARLLRDVAEYAGSTGVYRILTESNPRSRDWELADEVHFVPRGMEHPTRIARER
CEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
HVDGVVPLIGVDDPLPAVGEMKEELEAEGIRVVASDRESVEIGIDKVRTKRAFEELNVPT
CCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCEEHHHHHHHHHHHHHHCCCCC
PEWEVVENERPSISPPVVVKDPRSQAGLGVTVHETHKPRVSGRKLVEEFIEGAEVSIEVL
CCCEEECCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEE
SWDGEVVPLVPVFKGSTADRRHPIDRMRMSPAPIDPSVERKIRRCAVRVVEHLGLEGNVD
ECCCCEEEEEEEECCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
FDVVVRENEFWFLEFNPRPSGTRYMTSGCTGIWPLRELVDMAADRWRPPKIRKLWCAIEC
EEEEEECCEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEEEC
PVWRPTDKDPLREMFDGGVYHVFYEYYGRFDVGGRVTVRGDSFKEALTRLRSALKAAGAD
CCCCCCCHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCH
VKRAEREYRRRLREIEEYL
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA