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The map label for this gene is aspC

Identifier: 20094147

GI number: 20094147

Start: 675105

End: 676400

Strand: Reverse

Name: aspC

Synonym: MK0710

Alternate gene names: 20094147

Gene position: 676400-675105 (Counterclockwise)

Preceding gene: 20094148

Following gene: 20094146

Centisome position: 39.91

GC content: 62.11

Gene sequence:

>1296_bases
GTGCTCAAGGACGCGTACACGGCCGACGTCACTCCCGAGCGGGACGGAGAGGAGGTGAGACTGGCGGGATGGGTGCACGA
GGTAAGGGACCTAGGAGGGATCAAGTTCGTCCTCCTGCGCGATCGTACGGGGATCGTACAGCTGACGCTTCCCAAGCAGA
AGGTACCGAAGGAAACGTTCGAGAAGGTGCCGAAGCTCACTAAGGAATCGGTAATCCGTGTCGAGGGTACGGTCCAGGCG
AACGAGAAGGCTCCGGGAGGCGTCGAGGTCATCCCGCAACGTATCGAGGTACTCTCGGAGTCGGACACGCACCTGCCCCT
GGACCCGACCGGTAAGGTAGACGCGGACCTCGATACCAGACTCGACGCACGGGTACTGGATCTCCGTCGCGAGGAACCCC
AGGCGATCTTCAAGATCAGGAACGTGGTGACCACCGCGATCCGGGAGTTCCTCGAGGAGCGCGGGTTCATCGAAGTTCAC
ACGCCGAAGATAATCGCCTCGGCGACGGAAGGTGGTACCGAGCTGTTCCCGGTGGTGTACTTCGAGAGGGATGCGTACCT
GGCGCAGAGCCCACAGCTTTACAAGCAGATGCTCATGGCCGCGGGCTTCGAGCGCGTTTACGAGATCGGGCCCATCTTCC
GCGCGGAGGAGCACAACACGCGGCGACACCTCAACGAGGCCATCTCCGTGGACATCGAGATGTCGTTCATAGAGAGCGAG
GAGGACGTGATGCGGGTACTCGAGGAGCTCCTGGCGCACGTCTTCCGGAAGGTGCGGGAGGAATGTGAGAAGGAGCTTGA
GGCATTGGATCGGGAGCTACCGGAGCTGGAGACACCGTTCGAGCGTATAACGTACGAGGAGACCCTAGACCTGCTGTCCG
AGCACGGTATCGAGGTCGAGTGGGGCGAGGACCTACCTACCGAGGCCGAGCGCAAGCTAGGGGAGATCTTTGAGGAGCCG
TTCTTCATCACCGAATGGCCTAGGGAGACCAGACCCTTCTACACGATGGCCAAGGACGACGAGGTGACGACGGCGTTCGA
CCTGATGTATCAGGGATTGGAGCTCGCCTCCGGGGCCCAGCGGGAACACCGTTACGACGTGCTCGTCCGGCAGATCGAAG
AGCAGGGGCTGAGCCCCGAGGACTTCCGGCACTACCTGGAGGCCTTCAAGTACGGTATGCCACCGCACGGCGGGTGGGGT
CTCGGACTGGAGCGCACCCTGATGACCATCACGGGCGCCGAGAACATCCGTGAGGTAACCCTGTTCCCGCGGGACCGCAA
ACGCCTGCACCCGTGA

Upstream 100 bases:

>100_bases
GCCGAGCGTGAGGGCCTACCGAACCACGCGGAGGCGGTGAGGGCGCGCAGGTCCTGAGGGTTCTTAAGGGCAGCCACGTG
CGCGGACGGGGGTGTGGAAG

Downstream 100 bases:

>100_bases
GGTGGGGTGCGGTGATCCGCGACGGTTGCCGTCTCAGACTCGCGCTGTTCCTCCCGGGCTCCGTAATGTTTCTCGCGTCC
ACGTGCCCCAGTTATCTTTG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MLKDAYTADVTPERDGEEVRLAGWVHEVRDLGGIKFVLLRDRTGIVQLTLPKQKVPKETFEKVPKLTKESVIRVEGTVQA
NEKAPGGVEVIPQRIEVLSESDTHLPLDPTGKVDADLDTRLDARVLDLRREEPQAIFKIRNVVTTAIREFLEERGFIEVH
TPKIIASATEGGTELFPVVYFERDAYLAQSPQLYKQMLMAAGFERVYEIGPIFRAEEHNTRRHLNEAISVDIEMSFIESE
EDVMRVLEELLAHVFRKVREECEKELEALDRELPELETPFERITYEETLDLLSEHGIEVEWGEDLPTEAERKLGEIFEEP
FFITEWPRETRPFYTMAKDDEVTTAFDLMYQGLELASGAQREHRYDVLVRQIEEQGLSPEDFRHYLEAFKYGMPPHGGWG
LGLERTLMTITGAENIREVTLFPRDRKRLHP

Sequences:

>Translated_431_residues
MLKDAYTADVTPERDGEEVRLAGWVHEVRDLGGIKFVLLRDRTGIVQLTLPKQKVPKETFEKVPKLTKESVIRVEGTVQA
NEKAPGGVEVIPQRIEVLSESDTHLPLDPTGKVDADLDTRLDARVLDLRREEPQAIFKIRNVVTTAIREFLEERGFIEVH
TPKIIASATEGGTELFPVVYFERDAYLAQSPQLYKQMLMAAGFERVYEIGPIFRAEEHNTRRHLNEAISVDIEMSFIESE
EDVMRVLEELLAHVFRKVREECEKELEALDRELPELETPFERITYEETLDLLSEHGIEVEWGEDLPTEAERKLGEIFEEP
FFITEWPRETRPFYTMAKDDEVTTAFDLMYQGLELASGAQREHRYDVLVRQIEEQGLSPEDFRHYLEAFKYGMPPHGGWG
LGLERTLMTITGAENIREVTLFPRDRKRLHP
>Mature_431_residues
MLKDAYTADVTPERDGEEVRLAGWVHEVRDLGGIKFVLLRDRTGIVQLTLPKQKVPKETFEKVPKLTKESVIRVEGTVQA
NEKAPGGVEVIPQRIEVLSESDTHLPLDPTGKVDADLDTRLDARVLDLRREEPQAIFKIRNVVTTAIREFLEERGFIEVH
TPKIIASATEGGTELFPVVYFERDAYLAQSPQLYKQMLMAAGFERVYEIGPIFRAEEHNTRRHLNEAISVDIEMSFIESE
EDVMRVLEELLAHVFRKVREECEKELEALDRELPELETPFERITYEETLDLLSEHGIEVEWGEDLPTEAERKLGEIFEEP
FFITEWPRETRPFYTMAKDDEVTTAFDLMYQGLELASGAQREHRYDVLVRQIEEQGLSPEDFRHYLEAFKYGMPPHGGWG
LGLERTLMTITGAENIREVTLFPRDRKRLHP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI45439306, Length=452, Percent_Identity=36.5044247787611, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI4758762, Length=436, Percent_Identity=31.8807339449541, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI226491502, Length=465, Percent_Identity=29.8924731182796, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI194272210, Length=464, Percent_Identity=29.0948275862069, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI5031815, Length=464, Percent_Identity=29.0948275862069, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI40789249, Length=248, Percent_Identity=31.8548387096774, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1787161, Length=454, Percent_Identity=29.9559471365639, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788173, Length=267, Percent_Identity=33.7078651685393, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790571, Length=454, Percent_Identity=27.7533039647577, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789256, Length=454, Percent_Identity=27.3127753303965, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=26.7692307692308, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17551876, Length=452, Percent_Identity=37.3893805309735, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI71984122, Length=435, Percent_Identity=31.9540229885057, Blast_Score=207, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI17556372, Length=403, Percent_Identity=28.5359801488834, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71994340, Length=460, Percent_Identity=26.0869565217391, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32566633, Length=302, Percent_Identity=28.8079470198675, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17535925, Length=460, Percent_Identity=26.0869565217391, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17535927, Length=398, Percent_Identity=26.6331658291457, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323011, Length=451, Percent_Identity=37.9157427937916, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6321807, Length=439, Percent_Identity=30.2961275626424, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319872, Length=456, Percent_Identity=27.1929824561404, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6320242, Length=464, Percent_Identity=28.448275862069, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324256, Length=385, Percent_Identity=26.4935064935065, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6325153, Length=257, Percent_Identity=31.5175097276265, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136276, Length=440, Percent_Identity=37.2727272727273, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI19921528, Length=440, Percent_Identity=32.9545454545455, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI19922416, Length=455, Percent_Identity=25.7142857142857, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24640851, Length=456, Percent_Identity=27.6315789473684, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640849, Length=456, Percent_Identity=27.6315789473684, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24584738, Length=310, Percent_Identity=31.9354838709677, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYD_METKA (Q8TXG4)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613994.1
- ProteinModelPortal:   Q8TXG4
- SMR:   Q8TXG4
- GeneID:   1476811
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0710
- NMPDR:   fig|190192.1.peg.707
- HOGENOM:   HBG745843
- OMA:   TDWPSEI
- ProtClustDB:   PRK05159
- BioCyc:   MKAN190192:MK0710-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_A
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00458

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 49792; Mature: 49792

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKDAYTADVTPERDGEEVRLAGWVHEVRDLGGIKFVLLRDRTGIVQLTLPKQKVPKETF
CCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCEEEEEEECCCCEEEEECCHHHCCHHHH
EKVPKLTKESVIRVEGTVQANEKAPGGVEVIPQRIEVLSESDTHLPLDPTGKVDADLDTR
HHHHHHHHHCEEEEECEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
LDARVLDLRREEPQAIFKIRNVVTTAIREFLEERGFIEVHTPKIIASATEGGTELFPVVY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCEEEEEEE
FERDAYLAQSPQLYKQMLMAAGFERVYEIGPIFRAEEHNTRRHLNEAISVDIEMSFIESE
EECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEEEEEECCCH
EDVMRVLEELLAHVFRKVREECEKELEALDRELPELETPFERITYEETLDLLSEHGIEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEE
WGEDLPTEAERKLGEIFEEPFFITEWPRETRPFYTMAKDDEVTTAFDLMYQGLELASGAQ
ECCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCH
REHRYDVLVRQIEEQGLSPEDFRHYLEAFKYGMPPHGGWGLGLERTLMTITGAENIREVT
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEEECCCCCEEEE
LFPRDRKRLHP
ECCCHHHCCCC
>Mature Secondary Structure
MLKDAYTADVTPERDGEEVRLAGWVHEVRDLGGIKFVLLRDRTGIVQLTLPKQKVPKETF
CCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCEEEEEEECCCCEEEEECCHHHCCHHHH
EKVPKLTKESVIRVEGTVQANEKAPGGVEVIPQRIEVLSESDTHLPLDPTGKVDADLDTR
HHHHHHHHHCEEEEECEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
LDARVLDLRREEPQAIFKIRNVVTTAIREFLEERGFIEVHTPKIIASATEGGTELFPVVY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCEEEEEEE
FERDAYLAQSPQLYKQMLMAAGFERVYEIGPIFRAEEHNTRRHLNEAISVDIEMSFIESE
EECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEEEEEECCCH
EDVMRVLEELLAHVFRKVREECEKELEALDRELPELETPFERITYEETLDLLSEHGIEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEE
WGEDLPTEAERKLGEIFEEPFFITEWPRETRPFYTMAKDDEVTTAFDLMYQGLELASGAQ
ECCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCH
REHRYDVLVRQIEEQGLSPEDFRHYLEAFKYGMPPHGGWGLGLERTLMTITGAENIREVT
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEEECCCCCEEEE
LFPRDRKRLHP
ECCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014