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The map label for this gene is RpoB_1 [H]

Identifier: 20094124

GI number: 20094124

Start: 646413

End: 648335

Strand: Reverse

Name: RpoB_1 [H]

Synonym: MK0686

Alternate gene names: 20094124

Gene position: 648335-646413 (Counterclockwise)

Preceding gene: 20094125

Following gene: 20094123

Centisome position: 38.25

GC content: 62.45

Gene sequence:

>1923_bases
GTGGAGACCCGTGTGGATAAGGCCGTTGAGCATGTTCTCAAGGGTCCCGAACGCGAGGAAGATAGAACCCCGGTGTACCT
GAACGGACGGCTGATCGGATACGTGGAGGACCCGGAGGAGTTCGTGGAGAAAGTCCGGGAGTTGCGACGGGAGGGCGTGC
TGCATCCGAGGGTCAACGCCGCCTACCACGAGGACCTGAACGAGGTGCACGTGAACTGTGACCGTGGACGCGTGGTCCGT
CCGCTACTGGTGGTGGAGGACGGCGAGGTCAAGCTCACGGAGGAGCACCTGGAGAAGCTCCGAGAAGGAGAGATCTCGTG
GAAGGACCTGGAGGAAGAAGGCGTCATCGAGTTCCTGGACGCCGAAGAGGAGGAGAACGCGCTCATCGCCCAGTCCCTGG
AGGAGTTCTACTCGATGCCTAAGGAGGAGCGGAAGAAGTACACACATGTGGAGCTGGACCCGGCGGCGATATTCGGAACG
TGCTGCAGCTGCATCCCCTGGCCGGAGTCGAACTCGGCGCCGCGTAACACGATGGGTACGAACATGACCCGGCAGGCGCT
GGGCTTCTACGCCGCGAACCACCCCATCCGGATGGAGTCCCGGGGTCACCTGCTGTACTACCCGCACCGACCGCTCGTGA
AGACCAGAGGTACGGAAGCTTTCGACTACGACGAGCGGCCGGCCGGCCAGAACATGATCGTCGCCGTGCTCACGTACGAG
GGTTACAACATGGAGGACGCCATCATCATGAACGAGTCCGCGATCGAGCGCGGTCTGGCTAGGTCGTTCTTCTTCAAGAC
GTTCGAAGACGAGGCACGGACGTATCCGGGCGGGATGAAGGACAAGTTCTGCATCCCGGAGAAGGAGGTGCGTGGATACC
GGTCCGAGGACGCGTACAAGCACCTGGGAGAGGATGGACTGGCGGAGGTCGGGGAGCGCGTCGAGGGTCGTGACGTGATC
ATCGGGAAGACGAGCCCGCCGCGCTTCCTGGAGGAATTCCGACCGGAGGAACTGCCGGCGGAGGAGAGGCGTGAGTCCTC
CACGGCCATCCGGCACACCGACTCCGGTATCGTGGACTCCGTGATCATCACCGAGACCAGCGACGGACACAAGCTGGTGA
AGGTGAGGATCAGGGACGAGCGGGTGCCGGAGCTCGGTGACAAGTTCTCCTCACGGCACGGTCAGAAAGGAGTCATCGGG
ATGATCGTCCCGGAGGAGGACATGCCGTTCACCGAGGACGGGATCACACCGGACCTGATACTCAACCCGCACGCGCTACC
GTCGCGTGAGACCATGGCCCACATTCTGGAGACGCTCGCGGGTAAGGCGGCGGCACTGAGCGGGAAGAGGATCGACGGTT
CCGCGTTCACCTCGAACCCGAAGGAGGCCTACGAGGAGATCAAGAAGATGCTCCGCGAGCTGGGGTTCGAGCCCACCGGT
AAGGAGGTCATGTACGACGGCCGTACCGGGCAGAAGCTCGAAGCGGAGGTCTTCATCGGAGTGTGCCTGTACCGGAAGCT
GTACCACATGGTGAAGGACAAGTACCACGCGAGATCGCGCGGTCCTGTGCAGGTACTCACCAGGCAGCCGACGGAAGGAA
GGGCCCGGGAGGGCGGTCTCAGGTTCGGAGAGATGGAACGCGACGACCTCGTGGCTTGGGGCGCCTCATTGTTCCTGAAA
GAGAGGCTGCTCGACGAGTCCGACAAGTACGAGGCGTACATTTGCCCGAACTGCGGAGAGCTGTGTTACGTGCACGCCGC
GACGGGTAAGACGATCTGTCCCATCTGCGGCGAGGTGCGGGCTGACCGCGTCGAGATGTCGTACGCGTTCAAGATTCTGC
TGGACGAGCTGAAGTCGTTCTGCATGGACGCCGTGCTGGAAGTCGGCTCGCTGTCCGAACGGGAGGAGGGTGAGGAGGAA
TGA

Upstream 100 bases:

>100_bases
GAGGACGAGGTGATCGAAATCCTGGAGGAGATCGGGCTCGAAACCGAGTAAGGGGGACCCCGAACCCGTGTCGAACGAGG
AGGGTGGCGTGCGGGACGAC

Downstream 100 bases:

>100_bases
CCCTGATGGAGGAACCTCGCGTTCAAGATCCACCCAAGTACGTGAAGCGCATCCTGTTCGGGATCCTACCGCCCGACAAG
ATCCGGAAGATGTCCAAGGT

Product: DNA-directed RNA polymerase subunit B'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
METRVDKAVEHVLKGPEREEDRTPVYLNGRLIGYVEDPEEFVEKVRELRREGVLHPRVNAAYHEDLNEVHVNCDRGRVVR
PLLVVEDGEVKLTEEHLEKLREGEISWKDLEEEGVIEFLDAEEEENALIAQSLEEFYSMPKEERKKYTHVELDPAAIFGT
CCSCIPWPESNSAPRNTMGTNMTRQALGFYAANHPIRMESRGHLLYYPHRPLVKTRGTEAFDYDERPAGQNMIVAVLTYE
GYNMEDAIIMNESAIERGLARSFFFKTFEDEARTYPGGMKDKFCIPEKEVRGYRSEDAYKHLGEDGLAEVGERVEGRDVI
IGKTSPPRFLEEFRPEELPAEERRESSTAIRHTDSGIVDSVIITETSDGHKLVKVRIRDERVPELGDKFSSRHGQKGVIG
MIVPEEDMPFTEDGITPDLILNPHALPSRETMAHILETLAGKAAALSGKRIDGSAFTSNPKEAYEEIKKMLRELGFEPTG
KEVMYDGRTGQKLEAEVFIGVCLYRKLYHMVKDKYHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDDLVAWGASLFLK
ERLLDESDKYEAYICPNCGELCYVHAATGKTICPICGEVRADRVEMSYAFKILLDELKSFCMDAVLEVGSLSEREEGEEE

Sequences:

>Translated_640_residues
METRVDKAVEHVLKGPEREEDRTPVYLNGRLIGYVEDPEEFVEKVRELRREGVLHPRVNAAYHEDLNEVHVNCDRGRVVR
PLLVVEDGEVKLTEEHLEKLREGEISWKDLEEEGVIEFLDAEEEENALIAQSLEEFYSMPKEERKKYTHVELDPAAIFGT
CCSCIPWPESNSAPRNTMGTNMTRQALGFYAANHPIRMESRGHLLYYPHRPLVKTRGTEAFDYDERPAGQNMIVAVLTYE
GYNMEDAIIMNESAIERGLARSFFFKTFEDEARTYPGGMKDKFCIPEKEVRGYRSEDAYKHLGEDGLAEVGERVEGRDVI
IGKTSPPRFLEEFRPEELPAEERRESSTAIRHTDSGIVDSVIITETSDGHKLVKVRIRDERVPELGDKFSSRHGQKGVIG
MIVPEEDMPFTEDGITPDLILNPHALPSRETMAHILETLAGKAAALSGKRIDGSAFTSNPKEAYEEIKKMLRELGFEPTG
KEVMYDGRTGQKLEAEVFIGVCLYRKLYHMVKDKYHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDDLVAWGASLFLK
ERLLDESDKYEAYICPNCGELCYVHAATGKTICPICGEVRADRVEMSYAFKILLDELKSFCMDAVLEVGSLSEREEGEEE
>Mature_640_residues
METRVDKAVEHVLKGPEREEDRTPVYLNGRLIGYVEDPEEFVEKVRELRREGVLHPRVNAAYHEDLNEVHVNCDRGRVVR
PLLVVEDGEVKLTEEHLEKLREGEISWKDLEEEGVIEFLDAEEEENALIAQSLEEFYSMPKEERKKYTHVELDPAAIFGT
CCSCIPWPESNSAPRNTMGTNMTRQALGFYAANHPIRMESRGHLLYYPHRPLVKTRGTEAFDYDERPAGQNMIVAVLTYE
GYNMEDAIIMNESAIERGLARSFFFKTFEDEARTYPGGMKDKFCIPEKEVRGYRSEDAYKHLGEDGLAEVGERVEGRDVI
IGKTSPPRFLEEFRPEELPAEERRESSTAIRHTDSGIVDSVIITETSDGHKLVKVRIRDERVPELGDKFSSRHGQKGVIG
MIVPEEDMPFTEDGITPDLILNPHALPSRETMAHILETLAGKAAALSGKRIDGSAFTSNPKEAYEEIKKMLRELGFEPTG
KEVMYDGRTGQKLEAEVFIGVCLYRKLYHMVKDKYHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDDLVAWGASLFLK
ERLLDESDKYEAYICPNCGELCYVHAATGKTICPICGEVRADRVEMSYAFKILLDELKSFCMDAVLEVGSLSEREEGEEE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=617, Percent_Identity=41.4910858995138, Blast_Score=502, Evalue=1e-142,
Organism=Homo sapiens, GI238908503, Length=612, Percent_Identity=36.7647058823529, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI238908505, Length=612, Percent_Identity=36.7647058823529, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI212286172, Length=499, Percent_Identity=34.4689378757515, Blast_Score=292, Evalue=6e-79,
Organism=Homo sapiens, GI33469941, Length=499, Percent_Identity=34.4689378757515, Blast_Score=291, Evalue=9e-79,
Organism=Escherichia coli, GI1790419, Length=252, Percent_Identity=37.3015873015873, Blast_Score=136, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17552304, Length=625, Percent_Identity=41.44, Blast_Score=514, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI25144348, Length=624, Percent_Identity=38.3012820512821, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17506623, Length=588, Percent_Identity=31.2925170068027, Blast_Score=285, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6324725, Length=643, Percent_Identity=43.0793157076205, Blast_Score=524, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6324781, Length=628, Percent_Identity=39.6496815286624, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6325267, Length=471, Percent_Identity=35.244161358811, Blast_Score=276, Evalue=9e-75,
Organism=Drosophila melanogaster, GI17136444, Length=617, Percent_Identity=41.9773095623987, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17647877, Length=601, Percent_Identity=37.603993344426, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI17136446, Length=633, Percent_Identity=30.3317535545024, Blast_Score=279, Evalue=3e-75,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007120
- InterPro:   IPR019969
- InterPro:   IPR007121
- InterPro:   IPR007646
- InterPro:   IPR007647
- InterPro:   IPR007641
- InterPro:   IPR014724 [H]

Pfam domain/function: PF04566 RNA_pol_Rpb2_4; PF04567 RNA_pol_Rpb2_5; PF00562 RNA_pol_Rpb2_6; PF04560 RNA_pol_Rpb2_7 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 72787; Mature: 72787

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01166 RNA_POL_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METRVDKAVEHVLKGPEREEDRTPVYLNGRLIGYVEDPEEFVEKVRELRREGVLHPRVNA
CCCHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCH
AYHEDLNEVHVNCDRGRVVRPLLVVEDGEVKLTEEHLEKLREGEISWKDLEEEGVIEFLD
HHHHCHHHEEEECCCCCEEEEEEEEECCCEEECHHHHHHHHCCCCCHHCCCCCCCEEEEC
AEEEENALIAQSLEEFYSMPKEERKKYTHVELDPAAIFGTCCSCIPWPESNSAPRNTMGT
CCCCCCHHHHHHHHHHHCCCHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCC
NMTRQALGFYAANHPIRMESRGHLLYYPHRPLVKTRGTEAFDYDERPAGQNMIVAVLTYE
CHHHHHHHHHHCCCCEEECCCCCEEEECCCCCHHCCCCCCCCCCCCCCCCCEEEEEEEEC
GYNMEDAIIMNESAIERGLARSFFFKTFEDEARTYPGGMKDKFCIPEKEVRGYRSEDAYK
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCHHHHH
HLGEDGLAEVGERVEGRDVIIGKTSPPRFLEEFRPEELPAEERRESSTAIRHTDSGIVDS
HCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHEEECCCCCCCE
VIITETSDGHKLVKVRIRDERVPELGDKFSSRHGQKGVIGMIVPEEDMPFTEDGITPDLI
EEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEE
LNPHALPSRETMAHILETLAGKAAALSGKRIDGSAFTSNPKEAYEEIKKMLRELGFEPTG
ECCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
KEVMYDGRTGQKLEAEVFIGVCLYRKLYHMVKDKYHARSRGPVQVLTRQPTEGRAREGGL
CCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCCC
RFGEMERDDLVAWGASLFLKERLLDESDKYEAYICPNCGELCYVHAATGKTICPICGEVR
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCEECCHHHHHH
ADRVEMSYAFKILLDELKSFCMDAVLEVGSLSEREEGEEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
METRVDKAVEHVLKGPEREEDRTPVYLNGRLIGYVEDPEEFVEKVRELRREGVLHPRVNA
CCCHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCH
AYHEDLNEVHVNCDRGRVVRPLLVVEDGEVKLTEEHLEKLREGEISWKDLEEEGVIEFLD
HHHHCHHHEEEECCCCCEEEEEEEEECCCEEECHHHHHHHHCCCCCHHCCCCCCCEEEEC
AEEEENALIAQSLEEFYSMPKEERKKYTHVELDPAAIFGTCCSCIPWPESNSAPRNTMGT
CCCCCCHHHHHHHHHHHCCCHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCC
NMTRQALGFYAANHPIRMESRGHLLYYPHRPLVKTRGTEAFDYDERPAGQNMIVAVLTYE
CHHHHHHHHHHCCCCEEECCCCCEEEECCCCCHHCCCCCCCCCCCCCCCCCEEEEEEEEC
GYNMEDAIIMNESAIERGLARSFFFKTFEDEARTYPGGMKDKFCIPEKEVRGYRSEDAYK
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCHHHHH
HLGEDGLAEVGERVEGRDVIIGKTSPPRFLEEFRPEELPAEERRESSTAIRHTDSGIVDS
HCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHEEECCCCCCCE
VIITETSDGHKLVKVRIRDERVPELGDKFSSRHGQKGVIGMIVPEEDMPFTEDGITPDLI
EEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEE
LNPHALPSRETMAHILETLAGKAAALSGKRIDGSAFTSNPKEAYEEIKKMLRELGFEPTG
ECCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
KEVMYDGRTGQKLEAEVFIGVCLYRKLYHMVKDKYHARSRGPVQVLTRQPTEGRAREGGL
CCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCCC
RFGEMERDDLVAWGASLFLKERLLDESDKYEAYICPNCGELCYVHAATGKTICPICGEVR
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCEECCHHHHHH
ADRVEMSYAFKILLDELKSFCMDAVLEVGSLSEREEGEEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2843811; 2834336 [H]