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The map label for this gene is RpoB_2 [H]

Identifier: 20094125

GI number: 20094125

Start: 648385

End: 649962

Strand: Reverse

Name: RpoB_2 [H]

Synonym: MK0687

Alternate gene names: 20094125

Gene position: 649962-648385 (Counterclockwise)

Preceding gene: 20094126

Following gene: 20094124

Centisome position: 38.35

GC content: 60.65

Gene sequence:

>1578_bases
CTGAGTGAGGAGGATTATTTGGCGTTATTGGAGGGTTATTTACGTAGAAACGAGGAACTCCTGGGCGATGTAAAGACGCA
CCCACTCATCGCCCATCACTTCCTATCCTATCACTGGTTGATAGACGAGGGCCTGCAACAGCTGATCGAGCAGATGGAAC
CGATCGAACCGGAAGTGGAGGGAGCCAGCTACCGAATCGAGTTCGAGAGGATCGAGGTGGGAGAACCCTCCTCGATAGAG
CCGGACGGCGCGCGTCGGAAGATCTACCCGATGGAGGCACGCCTGAGGAACATGCTCTATTCCGCGCCCCTGTACGCGGA
GATCGTAATGTACGAGGACGGGGAGGAGGTCGAGCGGGACAACGTCTACGTCGGCGAGCTTCCGGTGATGGTCCGGTCGA
AGGTGTGTAACACCTACGGCCTGTCGGAGGAGGAGCTGATCGAAGTCGGCGAGGATCCGAAGGATCCGGGCGGCTACTTC
ATCATCAACGGGTCGGAACGCGTGCTGATGTCCCTAGAGGATATCGCGCCGAACCGACGGGTGAACGAGAAGATCCGGGA
GCGTGGTGTACTGGACGAGGTTCGTTCCAGGGTGTACTCCCGGGGCGAGCGGTACCGCGGTCCCGTGGACGTCATCGACC
GGAAGGGTCGCCTGGTCATCGATATGCCGGCCGTGTACCGGAAGTTCCCCGTGGTGATCCTATTACGGGCGCTGGGACTG
ACCGAGGAGGAGATCATGGAGGGCGTCGGTCCCGAGTTCGCCACGATCATGGCGGAGCAGATGAGGGTACTCGTGAAGGA
GGAAATCGGCAGCCAGGAGGACGCGCTGGAGTACATCGGCAGGTTGTCGAGACCCGACGAGAGCCGCGAGGAGCGAATTA
AGCGGGGTAAGGAGGTACTGGCCAAGTACTTCCTACCGCACATCGCCGAGGTCGACGACCTGGACGACGATGAGAAGCTG
AAGGAGGTCTTCCGGAAGAAGGCCGACGAGGTCCTCAAGATGGTGCGCGAGGTGCTGGAGCTGAAGTACCGGAGGGTCAA
GGTGAAGAGCGTCCGTGACCGCGACCATTACGCGAACAAGCGGATCAAGCTGGCGGGTGACCTACTGTACGAGGCTTACG
AGTACGCGTTCCAGCAGCTGGCCCGCGAGATGAGGTATCAGTTCGAGCGCGCGTACTCCCGGGGTCGTGAGCCCACTATC
CGGTACATCGTGCGTCCGGACAAGGTCACGGACCACATCCGCAGCTGTATGTCGACCGGTACCTGGAGGACCGGCCACTA
CCGGCCCAGAACCGGAGTCTCCCAGATTCTCGAGCGGTATACCTGGATGTCCACCATGTCGCACTTGCGGAGGATCAGGG
CCGCTACGGAGCTGGGTGTGGGTAAGTGGTCGATCCCGAAGGAGGCTCGTTACCTGCATCCGACGCACATGGGCCGGTTG
TGCCCGAACGAGACCCCCGAGGGGGCCAACTGTGGTGCGATCAAGAACCTGGCCCTGTACGCCGAGGTCGTTCACAAAGA
CGCGGATGAGGACGAGGTGATCGAAATCCTGGAGGAGATCGGGCTCGAAACCGAGTAA

Upstream 100 bases:

>100_bases
TCGTCCGAGACAGCCCGACCGCGGGTAAGACCGTGGTGTACCGGGTCGTGACCAATCCGCCCGAGTAGGGGGGAACCCGA
TGGCCGAACCCGTCAAGTCT

Downstream 100 bases:

>100_bases
GGGGGACCCCGAACCCGTGTCGAACGAGGAGGGTGGCGTGCGGGACGACGTGGAGACCCGTGTGGATAAGGCCGTTGAGC
ATGTTCTCAAGGGTCCCGAA

Product: DNA-directed RNA polymerase subunit beta''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MSEEDYLALLEGYLRRNEELLGDVKTHPLIAHHFLSYHWLIDEGLQQLIEQMEPIEPEVEGASYRIEFERIEVGEPSSIE
PDGARRKIYPMEARLRNMLYSAPLYAEIVMYEDGEEVERDNVYVGELPVMVRSKVCNTYGLSEEELIEVGEDPKDPGGYF
IINGSERVLMSLEDIAPNRRVNEKIRERGVLDEVRSRVYSRGERYRGPVDVIDRKGRLVIDMPAVYRKFPVVILLRALGL
TEEEIMEGVGPEFATIMAEQMRVLVKEEIGSQEDALEYIGRLSRPDESREERIKRGKEVLAKYFLPHIAEVDDLDDDEKL
KEVFRKKADEVLKMVREVLELKYRRVKVKSVRDRDHYANKRIKLAGDLLYEAYEYAFQQLAREMRYQFERAYSRGREPTI
RYIVRPDKVTDHIRSCMSTGTWRTGHYRPRTGVSQILERYTWMSTMSHLRRIRAATELGVGKWSIPKEARYLHPTHMGRL
CPNETPEGANCGAIKNLALYAEVVHKDADEDEVIEILEEIGLETE

Sequences:

>Translated_525_residues
MSEEDYLALLEGYLRRNEELLGDVKTHPLIAHHFLSYHWLIDEGLQQLIEQMEPIEPEVEGASYRIEFERIEVGEPSSIE
PDGARRKIYPMEARLRNMLYSAPLYAEIVMYEDGEEVERDNVYVGELPVMVRSKVCNTYGLSEEELIEVGEDPKDPGGYF
IINGSERVLMSLEDIAPNRRVNEKIRERGVLDEVRSRVYSRGERYRGPVDVIDRKGRLVIDMPAVYRKFPVVILLRALGL
TEEEIMEGVGPEFATIMAEQMRVLVKEEIGSQEDALEYIGRLSRPDESREERIKRGKEVLAKYFLPHIAEVDDLDDDEKL
KEVFRKKADEVLKMVREVLELKYRRVKVKSVRDRDHYANKRIKLAGDLLYEAYEYAFQQLAREMRYQFERAYSRGREPTI
RYIVRPDKVTDHIRSCMSTGTWRTGHYRPRTGVSQILERYTWMSTMSHLRRIRAATELGVGKWSIPKEARYLHPTHMGRL
CPNETPEGANCGAIKNLALYAEVVHKDADEDEVIEILEEIGLETE
>Mature_524_residues
SEEDYLALLEGYLRRNEELLGDVKTHPLIAHHFLSYHWLIDEGLQQLIEQMEPIEPEVEGASYRIEFERIEVGEPSSIEP
DGARRKIYPMEARLRNMLYSAPLYAEIVMYEDGEEVERDNVYVGELPVMVRSKVCNTYGLSEEELIEVGEDPKDPGGYFI
INGSERVLMSLEDIAPNRRVNEKIRERGVLDEVRSRVYSRGERYRGPVDVIDRKGRLVIDMPAVYRKFPVVILLRALGLT
EEEIMEGVGPEFATIMAEQMRVLVKEEIGSQEDALEYIGRLSRPDESREERIKRGKEVLAKYFLPHIAEVDDLDDDEKLK
EVFRKKADEVLKMVREVLELKYRRVKVKSVRDRDHYANKRIKLAGDLLYEAYEYAFQQLAREMRYQFERAYSRGREPTIR
YIVRPDKVTDHIRSCMSTGTWRTGHYRPRTGVSQILERYTWMSTMSHLRRIRAATELGVGKWSIPKEARYLHPTHMGRLC
PNETPEGANCGAIKNLALYAEVVHKDADEDEVIEILEEIGLETE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=531, Percent_Identity=33.1450094161959, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI238908503, Length=512, Percent_Identity=29.296875, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI238908505, Length=472, Percent_Identity=30.2966101694915, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI33469941, Length=526, Percent_Identity=23.0038022813688, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1790419, Length=175, Percent_Identity=24, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17552304, Length=531, Percent_Identity=32.391713747646, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI25144348, Length=521, Percent_Identity=29.1746641074856, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17506623, Length=539, Percent_Identity=22.2634508348794, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324725, Length=568, Percent_Identity=30.8098591549296, Blast_Score=239, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6324781, Length=503, Percent_Identity=29.6222664015905, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6325267, Length=511, Percent_Identity=22.8962818003914, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136444, Length=531, Percent_Identity=32.7683615819209, Blast_Score=255, Evalue=6e-68,
Organism=Drosophila melanogaster, GI17647877, Length=509, Percent_Identity=26.7190569744597, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007644
- InterPro:   IPR007642
- InterPro:   IPR007645 [H]

Pfam domain/function: PF04563 RNA_pol_Rpb2_1; PF04561 RNA_pol_Rpb2_2; PF04565 RNA_pol_Rpb2_3 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 61122; Mature: 60990

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEDYLALLEGYLRRNEELLGDVKTHPLIAHHFLSYHWLIDEGLQQLIEQMEPIEPEVE
CCCHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GASYRIEFERIEVGEPSSIEPDGARRKIYPMEARLRNMLYSAPLYAEIVMYEDGEEVERD
CCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHC
NVYVGELPVMVRSKVCNTYGLSEEELIEVGEDPKDPGGYFIINGSERVLMSLEDIAPNRR
CEEECCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCH
VNEKIRERGVLDEVRSRVYSRGERYRGPVDVIDRKGRLVIDMPAVYRKFPVVILLRALGL
HHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCC
TEEEIMEGVGPEFATIMAEQMRVLVKEEIGSQEDALEYIGRLSRPDESREERIKRGKEVL
CHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AKYFLPHIAEVDDLDDDEKLKEVFRKKADEVLKMVREVLELKYRRVKVKSVRDRDHYANK
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RIKLAGDLLYEAYEYAFQQLAREMRYQFERAYSRGREPTIRYIVRPDKVTDHIRSCMSTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCC
TWRTGHYRPRTGVSQILERYTWMSTMSHLRRIRAATELGVGKWSIPKEARYLHPTHMGRL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCC
CPNETPEGANCGAIKNLALYAEVVHKDADEDEVIEILEEIGLETE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEEDYLALLEGYLRRNEELLGDVKTHPLIAHHFLSYHWLIDEGLQQLIEQMEPIEPEVE
CCHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GASYRIEFERIEVGEPSSIEPDGARRKIYPMEARLRNMLYSAPLYAEIVMYEDGEEVERD
CCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHC
NVYVGELPVMVRSKVCNTYGLSEEELIEVGEDPKDPGGYFIINGSERVLMSLEDIAPNRR
CEEECCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCH
VNEKIRERGVLDEVRSRVYSRGERYRGPVDVIDRKGRLVIDMPAVYRKFPVVILLRALGL
HHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCC
TEEEIMEGVGPEFATIMAEQMRVLVKEEIGSQEDALEYIGRLSRPDESREERIKRGKEVL
CHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AKYFLPHIAEVDDLDDDEKLKEVFRKKADEVLKMVREVLELKYRRVKVKSVRDRDHYANK
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RIKLAGDLLYEAYEYAFQQLAREMRYQFERAYSRGREPTIRYIVRPDKVTDHIRSCMSTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCC
TWRTGHYRPRTGVSQILERYTWMSTMSHLRRIRAATELGVGKWSIPKEARYLHPTHMGRL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCC
CPNETPEGANCGAIKNLALYAEVVHKDADEDEVIEILEEIGLETE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]