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The map label for this gene is RIO1_1 [H]

Identifier: 20093952

GI number: 20093952

Start: 478065

End: 478856

Strand: Reverse

Name: RIO1_1 [H]

Synonym: MK0514

Alternate gene names: 20093952

Gene position: 478856-478065 (Counterclockwise)

Preceding gene: 20093953

Following gene: 20093951

Centisome position: 28.25

GC content: 56.82

Gene sequence:

>792_bases
CTGACTTTGTCTGAAGATAAATACTTCTCCGCGATCGAGGAACTGGAGCCGAAACCAGGAAAGGTCAAAAGACTGGAGAA
AACCCTGGAAGATCTGAAGGTAGAGGAGGAAGTCTTCGACCAGTACACCCTGATGACCCTGTACGAGCTGTCCAGGCGTG
GATACATCGACGGGCTCATGGGGTTCGTGAAGACAGGGAAAGAGGCCAACGTCGCCAGGGGAGTCCGTGGCGACGAGCTA
GTCGCCGTCAAGATTTACCGCGCCGCCACCAGCGACTTCCGCCGGATGTGGAGGTACATCCGAGGCGATCCCAGGTTCAA
GAACATCGGGCGCAAGCGACACCGTATCGTCTACGCCTGGACCGAGAAGGAGTTCAAGAACCTGACCCGCGCCTACGAGG
CAGGCGTCAGGTGTCCCGAGCCGATCGCTCACATGAACAACGTGCTGATTATGGAGTTCATCGGAGACGATGAAGGGAAC
CCGTACCCGCTGATGAAAGATAATCCCCCGGAGGAAGAGTACGCGGAGGACGTTCTCTCGAATATCCTCGATGACTACCG
CAGGATGTATCGCAAAGCCGAGATAGTCCACGGCGACCTCAGCGAGTACAACATCCTCTACGGGGGCAAGGGCGACTACA
GGATCATAGACTTCTCCCAAGGGGTCGTGCTGGATCACCCCATCGCCGAAGAGCTGTTGATACGTGACTTGCGAAACCTC
ATCCGATTCTTCGAGCGCTTAAGTGTCGACACCCCCACGTTAGAGGAGGCCCTCGAGTACGTCCGGGGGTGA

Upstream 100 bases:

>100_bases
TTACGTGGAGGTATACTAGGGTACAGGTCGACTGGCTGAAGCGCAAGGGTAAATTGGACGAACGTGTGACCAAGCTCCTT
GAGGAGATCATACCGGGCGC

Downstream 100 bases:

>100_bases
GCACTTGGCGGAGCTGGCCGATGAATTCTACGAGAGCGCCGAGCGCGTGAAAATCCCCAAAGATCGTATCGGAGTGTTGA
TAGGCAAGGATGGCGAGACC

Product: serine/threonine protein kinase

Products: NA

Alternate protein names: AfRio1 [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTLSEDKYFSAIEELEPKPGKVKRLEKTLEDLKVEEEVFDQYTLMTLYELSRRGYIDGLMGFVKTGKEANVARGVRGDEL
VAVKIYRAATSDFRRMWRYIRGDPRFKNIGRKRHRIVYAWTEKEFKNLTRAYEAGVRCPEPIAHMNNVLIMEFIGDDEGN
PYPLMKDNPPEEEYAEDVLSNILDDYRRMYRKAEIVHGDLSEYNILYGGKGDYRIIDFSQGVVLDHPIAEELLIRDLRNL
IRFFERLSVDTPTLEEALEYVRG

Sequences:

>Translated_263_residues
MTLSEDKYFSAIEELEPKPGKVKRLEKTLEDLKVEEEVFDQYTLMTLYELSRRGYIDGLMGFVKTGKEANVARGVRGDEL
VAVKIYRAATSDFRRMWRYIRGDPRFKNIGRKRHRIVYAWTEKEFKNLTRAYEAGVRCPEPIAHMNNVLIMEFIGDDEGN
PYPLMKDNPPEEEYAEDVLSNILDDYRRMYRKAEIVHGDLSEYNILYGGKGDYRIIDFSQGVVLDHPIAEELLIRDLRNL
IRFFERLSVDTPTLEEALEYVRG
>Mature_262_residues
TLSEDKYFSAIEELEPKPGKVKRLEKTLEDLKVEEEVFDQYTLMTLYELSRRGYIDGLMGFVKTGKEANVARGVRGDELV
AVKIYRAATSDFRRMWRYIRGDPRFKNIGRKRHRIVYAWTEKEFKNLTRAYEAGVRCPEPIAHMNNVLIMEFIGDDEGNP
YPLMKDNPPEEEYAEDVLSNILDDYRRMYRKAEIVHGDLSEYNILYGGKGDYRIIDFSQGVVLDHPIAEELLIRDLRNLI
RFFERLSVDTPTLEEALEYVRG

Specific function: Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis [H]

COG id: COG1718

COG function: function code TD; Serine/threonine protein kinase involved in cell cycle control

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI23510356, Length=230, Percent_Identity=41.7391304347826, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI22325377, Length=228, Percent_Identity=32.8947368421053, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI23510358, Length=146, Percent_Identity=45.8904109589041, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI115532073, Length=228, Percent_Identity=37.280701754386, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17556977, Length=254, Percent_Identity=32.2834645669291, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71989043, Length=134, Percent_Identity=36.5671641791045, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324693, Length=245, Percent_Identity=33.8775510204082, Blast_Score=119, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24662811, Length=236, Percent_Identity=37.7118644067797, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21356879, Length=236, Percent_Identity=37.7118644067797, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19920700, Length=259, Percent_Identity=32.046332046332, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR018934
- InterPro:   IPR000687
- InterPro:   IPR018935 [H]

Pfam domain/function: PF01163 RIO1 [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 30824; Mature: 30692

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00109 PROTEIN_KINASE_TYR ; PS50011 PROTEIN_KINASE_DOM ; PS01245 RIO1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSEDKYFSAIEELEPKPGKVKRLEKTLEDLKVEEEVFDQYTLMTLYELSRRGYIDGLM
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GFVKTGKEANVARGVRGDELVAVKIYRAATSDFRRMWRYIRGDPRFKNIGRKRHRIVYAW
HHHHCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHCEEEEEE
TEKEFKNLTRAYEAGVRCPEPIAHMNNVLIMEFIGDDEGNPYPLMKDNPPEEEYAEDVLS
CHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
NILDDYRRMYRKAEIVHGDLSEYNILYGGKGDYRIIDFSQGVVLDHPIAEELLIRDLRNL
HHHHHHHHHHHHHHHHCCCHHHCEEEECCCCCEEEEEECCCEEECCCHHHHHHHHHHHHH
IRFFERLSVDTPTLEEALEYVRG
HHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TLSEDKYFSAIEELEPKPGKVKRLEKTLEDLKVEEEVFDQYTLMTLYELSRRGYIDGLM
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GFVKTGKEANVARGVRGDELVAVKIYRAATSDFRRMWRYIRGDPRFKNIGRKRHRIVYAW
HHHHCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHCEEEEEE
TEKEFKNLTRAYEAGVRCPEPIAHMNNVLIMEFIGDDEGNPYPLMKDNPPEEEYAEDVLS
CHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
NILDDYRRMYRKAEIVHGDLSEYNILYGGKGDYRIIDFSQGVVLDHPIAEELLIRDLRNL
HHHHHHHHHHHHHHHHCCCHHHCEEEECCCCCEEEEEECCCEEECCCHHHHHHHHHHHHH
IRFFERLSVDTPTLEEALEYVRG
HHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]