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The map label for this gene is rsmB [H]

Identifier: 20093882

GI number: 20093882

Start: 414419

End: 415777

Strand: Reverse

Name: rsmB [H]

Synonym: MK0444

Alternate gene names: 20093882

Gene position: 415777-414419 (Counterclockwise)

Preceding gene: 20093885

Following gene: 20093881

Centisome position: 24.53

GC content: 64.24

Gene sequence:

>1359_bases
TTGAGCAGCCTCAGCCGACAGGAGCTCACGGCACGCGTGTTCGTCGAGTGGGAGGAGACGCGACGTTCGCTCCGTGAGTG
CGTTAAGAAGGTGCTGCTGAACGAGGGAGTCCGGGACCATCGCGTCTGGGGCACCATCCACGCGTACTGCTTCGAGCTGA
TGAAGCGCCTCCGCACGGTCGACGCGTTCCTGGAGGCGACGATACGGAACGCGAAGCTTTTCGACCTGGACCCGTGGGTT
AGGAACGCCCTCAGGGTCGGTACGTTCGAGATGAAGTTCAACGACGTGAAGCCCGCGATCGCCACGAACGAGGCCGTGAA
GATCGTCGTGGAGTGTGTCGGGGAGGGCCCCGCCCGGTTCGTGAACGCCGTCCTCTACGACGTCGAGCGGCTCGAGCTCT
CCGACGTATTGAACCGGGCTAAGGACACCGTAGAGCGCCTGGCGATCGAGTACTCCCATCCGGAATGGTTCGTCAAGCAC
CTGATGGACCTACTGGGTGAAAACGAGCTGACGAAGTTGATGGACGCTAACAACCGCGAGCCCGAGCGTTACCTCCGCGT
ACATGCCCACATGGTGGACCCGGACAAGGCTATACTGGCGCTGGAGGACGACGGGGTGGCCGTGGAGGAAGATCCCGATC
TGCCGTTCATGCTGCGGGTGGTGGATCACGATGTACCGCCCGTCAGGACCGAGCCTTACCGCCGAGGTTGGGTGGCTTAC
CAAGACAAGGCGTCCGCGGCCGCCGCTTACGCGCTGCGGCCGGAGCCCGGAGATCGTGTGCTCGACGCTTGTGCGGCCCC
CGGTGGGAAGTCGGCGTACCTGTATGCGCTGACGGAAGGCGAGATCGAGCTCACGTGTGTGGACGTGAACCCCAGGCGCC
TCAGGGAGATGCGTCGTAACTTCCGCCGATGGGGGATCGAAGCCAGGCTCCGCAGGGCGGACTCCTCCCGGCTCTACCGG
GAGACCGACGAGACGTTCGACCTCGCCCTCGTGGACCCACCGTGTTCGAGCTCCGGGGCTTACGTTCGGGCCCCCGAGGC
CAAGTGGACCGTCAAGTGGAGACACGTGAAGAGGTATGCCCGCGGCCAGCTCTCGATCCTACGTGGCGTCGCGCCGCTGG
TGGAAAGAACCCTCGTGTACTCCACGTGCTCGGTCACCGTAGTGGAGGACGAGTCCGTGGTACGCCGCTTCTTGCGCGAG
TTCGACGAGTTCGAGGTGGAGGAACCTTTCGGGTTCGGCTCGCCCGGCTTCGACTCCTGGATGAACGAGAGGTACCCGTG
GGCGGATCGAGTACGTAGGTTCTGGCCGCATCGGCACCGTACGGAGGGGTTCTTCGTAGCCCGGCTGGTCCGGAGCTAG

Upstream 100 bases:

>100_bases
ATCGCCACCGCCTCCGATACCCAGGACGGCGACGGCACTAAGCTCCTCGATCCCGGCCACTTCGGCCAAGTACAAGTCAC
TAGGACCCCGGGGTGATCTG

Downstream 100 bases:

>100_bases
CGCGATGATGGCTTATCCCGGTGGAGGACCTTCACCATAGGGGGACGTCCGTGTCCGAGATCGAGAACCTGCGTGAGACC
ATCGAGAACATCCGCGAGGA

Product: tRNA/rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSSLSRQELTARVFVEWEETRRSLRECVKKVLLNEGVRDHRVWGTIHAYCFELMKRLRTVDAFLEATIRNAKLFDLDPWV
RNALRVGTFEMKFNDVKPAIATNEAVKIVVECVGEGPARFVNAVLYDVERLELSDVLNRAKDTVERLAIEYSHPEWFVKH
LMDLLGENELTKLMDANNREPERYLRVHAHMVDPDKAILALEDDGVAVEEDPDLPFMLRVVDHDVPPVRTEPYRRGWVAY
QDKASAAAAYALRPEPGDRVLDACAAPGGKSAYLYALTEGEIELTCVDVNPRRLREMRRNFRRWGIEARLRRADSSRLYR
ETDETFDLALVDPPCSSSGAYVRAPEAKWTVKWRHVKRYARGQLSILRGVAPLVERTLVYSTCSVTVVEDESVVRRFLRE
FDEFEVEEPFGFGSPGFDSWMNERYPWADRVRRFWPHRHRTEGFFVARLVRS

Sequences:

>Translated_452_residues
MSSLSRQELTARVFVEWEETRRSLRECVKKVLLNEGVRDHRVWGTIHAYCFELMKRLRTVDAFLEATIRNAKLFDLDPWV
RNALRVGTFEMKFNDVKPAIATNEAVKIVVECVGEGPARFVNAVLYDVERLELSDVLNRAKDTVERLAIEYSHPEWFVKH
LMDLLGENELTKLMDANNREPERYLRVHAHMVDPDKAILALEDDGVAVEEDPDLPFMLRVVDHDVPPVRTEPYRRGWVAY
QDKASAAAAYALRPEPGDRVLDACAAPGGKSAYLYALTEGEIELTCVDVNPRRLREMRRNFRRWGIEARLRRADSSRLYR
ETDETFDLALVDPPCSSSGAYVRAPEAKWTVKWRHVKRYARGQLSILRGVAPLVERTLVYSTCSVTVVEDESVVRRFLRE
FDEFEVEEPFGFGSPGFDSWMNERYPWADRVRRFWPHRHRTEGFFVARLVRS
>Mature_451_residues
SSLSRQELTARVFVEWEETRRSLRECVKKVLLNEGVRDHRVWGTIHAYCFELMKRLRTVDAFLEATIRNAKLFDLDPWVR
NALRVGTFEMKFNDVKPAIATNEAVKIVVECVGEGPARFVNAVLYDVERLELSDVLNRAKDTVERLAIEYSHPEWFVKHL
MDLLGENELTKLMDANNREPERYLRVHAHMVDPDKAILALEDDGVAVEEDPDLPFMLRVVDHDVPPVRTEPYRRGWVAYQ
DKASAAAAYALRPEPGDRVLDACAAPGGKSAYLYALTEGEIELTCVDVNPRRLREMRRNFRRWGIEARLRRADSSRLYRE
TDETFDLALVDPPCSSSGAYVRAPEAKWTVKWRHVKRYARGQLSILRGVAPLVERTLVYSTCSVTVVEDESVVRRFLREF
DEFEVEEPFGFGSPGFDSWMNERYPWADRVRRFWPHRHRTEGFFVARLVRS

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=328, Percent_Identity=31.7073170731707, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI76150623, Length=328, Percent_Identity=31.7073170731707, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI270288818, Length=234, Percent_Identity=32.4786324786325, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI8922322, Length=234, Percent_Identity=32.4786324786325, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI270288816, Length=234, Percent_Identity=32.4786324786325, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI23199998, Length=234, Percent_Identity=32.4786324786325, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI32698918, Length=190, Percent_Identity=28.9473684210526, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI2367212, Length=411, Percent_Identity=26.0340632603406, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081985, Length=236, Percent_Identity=27.5423728813559, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17536757, Length=316, Percent_Identity=30.379746835443, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71998419, Length=220, Percent_Identity=30, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115534021, Length=224, Percent_Identity=29.9107142857143, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324268, Length=242, Percent_Identity=28.9256198347107, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI22024126, Length=335, Percent_Identity=28.955223880597, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19920494, Length=178, Percent_Identity=30.8988764044944, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 52465; Mature: 52334

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLSRQELTARVFVEWEETRRSLRECVKKVLLNEGVRDHRVWGTIHAYCFELMKRLRTV
CCCCCHHHCCHHEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DAFLEATIRNAKLFDLDPWVRNALRVGTFEMKFNDVKPAIATNEAVKIVVECVGEGPARF
HHHHHHHHCCCEEEECCHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHH
VNAVLYDVERLELSDVLNRAKDTVERLAIEYSHPEWFVKHLMDLLGENELTKLMDANNRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCCCC
PERYLRVHAHMVDPDKAILALEDDGVAVEEDPDLPFMLRVVDHDVPPVRTEPYRRGWVAY
HHHHHHHHHEEECCCCEEEEECCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCEEE
QDKASAAAAYALRPEPGDRVLDACAAPGGKSAYLYALTEGEIELTCVDVNPRRLREMRRN
CCCCCHHEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHH
FRRWGIEARLRRADSSRLYRETDETFDLALVDPPCSSSGAYVRAPEAKWTVKWRHVKRYA
HHHHCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCEEEEHHHHHHHH
RGQLSILRGVAPLVERTLVYSTCSVTVVEDESVVRRFLREFDEFEVEEPFGFGSPGFDSW
HHHHHHHHHHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
MNERYPWADRVRRFWPHRHRTEGFFVARLVRS
HCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SSLSRQELTARVFVEWEETRRSLRECVKKVLLNEGVRDHRVWGTIHAYCFELMKRLRTV
CCCCHHHCCHHEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DAFLEATIRNAKLFDLDPWVRNALRVGTFEMKFNDVKPAIATNEAVKIVVECVGEGPARF
HHHHHHHHCCCEEEECCHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHH
VNAVLYDVERLELSDVLNRAKDTVERLAIEYSHPEWFVKHLMDLLGENELTKLMDANNRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCCCC
PERYLRVHAHMVDPDKAILALEDDGVAVEEDPDLPFMLRVVDHDVPPVRTEPYRRGWVAY
HHHHHHHHHEEECCCCEEEEECCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCEEE
QDKASAAAAYALRPEPGDRVLDACAAPGGKSAYLYALTEGEIELTCVDVNPRRLREMRRN
CCCCCHHEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHH
FRRWGIEARLRRADSSRLYRETDETFDLALVDPPCSSSGAYVRAPEAKWTVKWRHVKRYA
HHHHCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCEEEEHHHHHHHH
RGQLSILRGVAPLVERTLVYSTCSVTVVEDESVVRRFLREFDEFEVEEPFGFGSPGFDSW
HHHHHHHHHHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
MNERYPWADRVRRFWPHRHRTEGFFVARLVRS
HCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]