Click here to switch to the map view.

The map label for this gene is comE [H]

Identifier: 20093831

GI number: 20093831

Start: 376299

End: 376865

Strand: Reverse

Name: comE [H]

Synonym: MK0393

Alternate gene names: 20093831

Gene position: 376865-376299 (Counterclockwise)

Preceding gene: 20093834

Following gene: 20093830

Centisome position: 22.23

GC content: 58.91

Gene sequence:

>567_bases
ATGGGCATGAAGCGAATTGAAGCTCTGAGAGTAGTTGCCGACGTGGCCGAGCGGTACGATGCCGTAGTGACGGTCCACCT
GGGTTTCCCGGCCCGGGAGCTCTACCGTGTTAACGACCGGCGCCTCAACTTCTACATGTTGGGGGCGATGGGTCAGTCCT
GCTCGGTCGGTCTCGGACTGGCGCTCTGTACCGATCGGGAGGTACTGGCGATCGAAGGAGACGGCGGTCTTATGATGAAC
ATGGGGGTTCTCCCTACGATCGCTCAAGAGAGACCGCGTAACTATACCCTCGTGCTCATCGACAACTCCACGTACGCCAC
TACAGGCGATCAGCCTACGCCGTCCGATCGCATCGATTGGGAGAAGGTGGCTGAGGCCCATGGGTTGACGTACTTCGAGG
CCTCCGAACCCGAGTCCGCTGAAGTAGCCTTAGAAGATGCGCTCGCGACGGAAGGTCCTCGTATGGTAAGGCTAGAGGTC
GAGCCTGGAAACGCTGACGTTCCCTTAATAGATTTGGATCCGGAGGAGATCAAGGTGCGGTTCGTTCGAGCGCTGCGGGA
GGGATGA

Upstream 100 bases:

>100_bases
GGGAATCTTCACGGACTTCTCGAACGCCTTCACCGGGCGCCCTCCGGCCGATCCGGGGGGAACGGCTTTAGTGTATTCGG
GACCGGGCGACGCGGGGTCG

Downstream 100 bases:

>100_bases
TCGTTGAAAATACGGCCTGAGGATTTGACGGACTTCGTCAGTGAAGCTCTCCACGCCGTCGGCGTGCCGCGTAAAGACGC
TCGGACCGCCGCCGAAGTCA

Product: thiamine pyrophosphate-requiring enzyme

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MGMKRIEALRVVADVAERYDAVVTVHLGFPARELYRVNDRRLNFYMLGAMGQSCSVGLGLALCTDREVLAIEGDGGLMMN
MGVLPTIAQERPRNYTLVLIDNSTYATTGDQPTPSDRIDWEKVAEAHGLTYFEASEPESAEVALEDALATEGPRMVRLEV
EPGNADVPLIDLDPEEIKVRFVRALREG

Sequences:

>Translated_188_residues
MGMKRIEALRVVADVAERYDAVVTVHLGFPARELYRVNDRRLNFYMLGAMGQSCSVGLGLALCTDREVLAIEGDGGLMMN
MGVLPTIAQERPRNYTLVLIDNSTYATTGDQPTPSDRIDWEKVAEAHGLTYFEASEPESAEVALEDALATEGPRMVRLEV
EPGNADVPLIDLDPEEIKVRFVRALREG
>Mature_187_residues
GMKRIEALRVVADVAERYDAVVTVHLGFPARELYRVNDRRLNFYMLGAMGQSCSVGLGLALCTDREVLAIEGDGGLMMNM
GVLPTIAQERPRNYTLVLIDNSTYATTGDQPTPSDRIDWEKVAEAHGLTYFEASEPESAEVALEDALATEGPRMVRLEVE
PGNADVPLIDLDPEEIKVRFVRALREG

Specific function: Catalyzes the decarboxylation of sulfopyruvic acid to sulfoacetaldehyde [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022494
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 20755; Mature: 20624

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMKRIEALRVVADVAERYDAVVTVHLGFPARELYRVNDRRLNFYMLGAMGQSCSVGLGL
CCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHEECCCCEEEEEEEECCCCCCCCCCEE
ALCTDREVLAIEGDGGLMMNMGVLPTIAQERPRNYTLVLIDNSTYATTGDQPTPSDRIDW
EEECCCEEEEEECCCCEEEECCCCCCHHHCCCCCEEEEEECCCEEEECCCCCCCCCCCCH
EKVAEAHGLTYFEASEPESAEVALEDALATEGPRMVRLEVEPGNADVPLIDLDPEEIKVR
HHHHHHCCCEEEECCCCCCHHEEEHHHHCCCCCEEEEEEECCCCCCCEEEECCHHHHHHH
FVRALREG
HHHHHHCC
>Mature Secondary Structure 
GMKRIEALRVVADVAERYDAVVTVHLGFPARELYRVNDRRLNFYMLGAMGQSCSVGLGL
CHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHEECCCCEEEEEEEECCCCCCCCCCEE
ALCTDREVLAIEGDGGLMMNMGVLPTIAQERPRNYTLVLIDNSTYATTGDQPTPSDRIDW
EEECCCEEEEEECCCCEEEECCCCCCHHHCCCCCEEEEEECCCEEEECCCCCCCCCCCCH
EKVAEAHGLTYFEASEPESAEVALEDALATEGPRMVRLEVEPGNADVPLIDLDPEEIKVR
HHHHHHCCCEEEECCCCCCHHEEEHHHHCCCCCEEEEEEECCCCCCCEEEECCHHHHHHH
FVRALREG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]