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The map label for this gene is SEN2_1

Identifier: 20093779

GI number: 20093779

Start: 333200

End: 333739

Strand: Direct

Name: SEN2_1

Synonym: MK0341

Alternate gene names: 20093779

Gene position: 333200-333739 (Clockwise)

Preceding gene: 20093777

Following gene: 20093780

Centisome position: 19.66

GC content: 56.67

Gene sequence:

>540_bases
ATGTTGTGCGCCGGGAACGGGGGTAAGGAATTGCCACGTGCTAAGGTCTTCGAAGGAGGTAGCTTAGTCTCCAAGGATTA
CGAGGACCTGAAGCGTCGGTACTTCGGAACGGAGCACGGTAACGTGCTTTTCCTGGATCCTTTCGAAACTGTGTACCTGA
CGGAGAAGGGTGAGATCGACCCGGAGACGCCGGAAGGCGAACCCATGAGTGTGGAAGAACTGCTTTCTTTCTTCGAGCGA
AGAAGGCCGGGGTTTCGGGCAGGATACGTCGTTTATCGGGATCTCACCGAGCGGGGATACGTGGTGAAGTCCGGGTTTAA
GTACGGAGGTAGGTTCCGGGTGTACGAGGAGGACCCGGACCGGGAACACTCTAAATACGTGGTGAGAGTGGTCGAGCCGG
ACACGGAACTGTCTACCCGTGACGTACTGCGGGCCACACGTTTGGCACATTCCGTCCGGAAAGACTTCGTCCTAGCCGTC
GTTGAGGACGTCGAAGAACCACGCATCGAGTACGTGATGTGGCGGTGGAAGAGGCTGTAA

Upstream 100 bases:

>100_bases
CCCGGACCGAGTCAGTGCCGGGCTCAATCTTCATCCACAGGATCCGCGACGCAGGCCTTTCATCACGCCCCCGGTAGCCC
GAACGATCCATCCTAGTTGA

Downstream 100 bases:

>100_bases
GGGGGACACTCCCATGGCCGAGCAGAAGCCGTACTACGGAGCCTGGCCGTGTCACCCACGAGATCTAGTCGAGGAGGGAG
TGACCACCAGCGCCCGGCGG

Product: tRNA splicing endonuclease

Products: NA

Alternate protein names: tRNA-intron endonuclease

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MLCAGNGGKELPRAKVFEGGSLVSKDYEDLKRRYFGTEHGNVLFLDPFETVYLTEKGEIDPETPEGEPMSVEELLSFFER
RRPGFRAGYVVYRDLTERGYVVKSGFKYGGRFRVYEEDPDREHSKYVVRVVEPDTELSTRDVLRATRLAHSVRKDFVLAV
VEDVEEPRIEYVMWRWKRL

Sequences:

>Translated_179_residues
MLCAGNGGKELPRAKVFEGGSLVSKDYEDLKRRYFGTEHGNVLFLDPFETVYLTEKGEIDPETPEGEPMSVEELLSFFER
RRPGFRAGYVVYRDLTERGYVVKSGFKYGGRFRVYEEDPDREHSKYVVRVVEPDTELSTRDVLRATRLAHSVRKDFVLAV
VEDVEEPRIEYVMWRWKRL
>Mature_179_residues
MLCAGNGGKELPRAKVFEGGSLVSKDYEDLKRRYFGTEHGNVLFLDPFETVYLTEKGEIDPETPEGEPMSVEELLSFFER
RRPGFRAGYVVYRDLTERGYVVKSGFKYGGRFRVYEEDPDREHSKYVVRVVEPDTELSTRDVLRATRLAHSVRKDFVLAV
VEDVEEPRIEYVMWRWKRL

Specific function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate

COG id: COG1676

COG function: function code J; tRNA splicing endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily

Homologues:

Organism=Homo sapiens, GI116875840, Length=98, Percent_Identity=34.6938775510204, Blast_Score=67, Evalue=6e-12,
Organism=Homo sapiens, GI116875842, Length=98, Percent_Identity=34.6938775510204, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENDA_METKA (Q8TGZ7)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613626.1
- ProteinModelPortal:   Q8TGZ7
- SMR:   Q8TGZ7
- GeneID:   1477644
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0341
- NMPDR:   fig|190192.1.peg.339
- OMA:   SEREHSK
- BioCyc:   MKAN190192:MK0341-MONOMER
- BRENDA:   3.1.27.9
- HAMAP:   MF_01833
- InterPro:   IPR011856
- InterPro:   IPR006676
- InterPro:   IPR006677
- InterPro:   IPR006678
- InterPro:   IPR016442
- Gene3D:   G3DSA:3.40.1350.10
- PIRSF:   PIRSF005285
- TIGRFAMs:   TIGR00324

Pfam domain/function: PF01974 tRNA_int_endo; PF02778 tRNA_int_endo_N; SSF53032 tRNA_int_endo_C; SSF55267 tRNA_int_endo_N

EC number: =3.1.27.9

Molecular weight: Translated: 20939; Mature: 20939

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: ACT_SITE 115-115 ACT_SITE 123-123 ACT_SITE 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCAGNGGKELPRAKVFEGGSLVSKDYEDLKRRYFGTEHGNVLFLDPFETVYLTEKGEID
CEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCC
PETPEGEPMSVEELLSFFERRRPGFRAGYVVYRDLTERGYVVKSGFKYGGRFRVYEEDPD
CCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCCCCEEEEECCCCC
REHSKYVVRVVEPDTELSTRDVLRATRLAHSVRKDFVLAVVEDVEEPRIEYVMWRWKRL
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLCAGNGGKELPRAKVFEGGSLVSKDYEDLKRRYFGTEHGNVLFLDPFETVYLTEKGEID
CEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCC
PETPEGEPMSVEELLSFFERRRPGFRAGYVVYRDLTERGYVVKSGFKYGGRFRVYEEDPD
CCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCCCCEEEEECCCCC
REHSKYVVRVVEPDTELSTRDVLRATRLAHSVRKDFVLAVVEDVEEPRIEYVMWRWKRL
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014