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The map label for this gene is 20093726

Identifier: 20093726

GI number: 20093726

Start: 277208

End: 278248

Strand: Reverse

Name: 20093726

Synonym: MK0286

Alternate gene names: NA

Gene position: 278248-277208 (Counterclockwise)

Preceding gene: 20093727

Following gene: 20093725

Centisome position: 16.42

GC content: 58.89

Gene sequence:

>1041_bases
ATGACCTGCGAACTCTGCGGGGATCCCGCATCCTCATTTTTACGCGTGTGCAGGGAGTGTATCCTGAACCGCTGGGATAA
AGCCCGAGAGATCCTCCGAGAGGCGCACGCTGAAGCGAGGAGAGAGCTTGGACTCCCACCGGAGCCGCCGTCGGAGGGGC
TCGAGTGCGGCGCCTGCGTGAACCGGTGTAGGATACCCGAAGGGAAGGTCGGGTACTGTGGCGTCGTTCGAAACGAGGGC
GGTAAGATAATCCGTGAGCACGCGGGCCGCGCGTATTTGGACCCCCACCCCACCAACTGCGTGGGAGCGTGGGTCTGCCC
CGGTGCCACCTCCCGCGGGTACCCGAAGTACACGGAAACACGGGGGCCCGAGCGCGGTCGGTATAACTTAGCCGTATTCT
ATGGAGGCTGCACCCACCACTGCTTCTTCTGCCAGAATTACGAGCACTGGGTAGAACGTGAGCGGCTGAGTGCCGAAGAT
TTGAAACGTCGTGCCTCCGATCCGGACGTGACGTGCGTATGTTTCTTCGGTGGTGATCCCACCCCCCATTACGACCAGGT
TATAGAGATCGGACGTGAGTGGGAGGAGCTCCGCGTTCGCGTGTGCTTGGAAACGAACGGAAGCTTGAGACCGAAACTCA
TGCGCAGAATCGCCGAGATCTGCTACGAGTCCGGTGGAGGTTTCAACATGGACGTCAAGGCGTACGACCCACGGATATAC
TACGCCCTATCCTTCTCCGATCCTGAGTACACGTTTAAGAACCTCGAGATCATCGGTCGGGAATTCGCGCGAGAAGATCC
ACCGATCCTCCGCCCGAGCACCCTAGTAGTGCCCGAGTACATTACGCCCGAGGAGGTAGAGAAAATCGCGGAGTTTCTGG
CTTCCATCGACGAACGTATACCTTACCGGCTTCTCCAGCACGTGCCTCAATACCACTCAGCGGACCTCGGTTACACGCCG
GTCTGTGTTATGCGAGAGTGTAAACGTGCCGCCGAACGTCATCTGGAGAACGTTGACGCCTGCATATACCGATACGCCTG
A

Upstream 100 bases:

>100_bases
TGATGATCGTGGAGCAGGTCAAGGCCACCCTCGAGTCCGCCTTCGAGGGCGTGAACGTCGACGTGAAGCTGGTCGGACAC
GTGTTGACGGAGGAAGAAGA

Downstream 100 bases:

>100_bases
GACTAATTCGAAATCTCAACCGAACGCGGGGAGGGAACGCGTTGACCCTGGTACTGGCCTACGCCGGGACGGACGGTGCT
CTCGTGGCCGGGGACCGTCG

Product: pyruvate-formate lyase-activating enzyme

Products: NA

Alternate protein names: Radical SAM Domain Protein; Pyruvate-Formate Lyase-Activating; Radical Sam Domain Protein; Pyruvate Formate-Lyase Activating Related Protein; Glutamate 5-Kinase; Pyruvate-Formate Lyase-Activating PflA; Pyruvate Formate-Lyase Activating -Like Protein; Pyruvate Formate-Lyase Activating

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTCELCGDPASSFLRVCRECILNRWDKAREILREAHAEARRELGLPPEPPSEGLECGACVNRCRIPEGKVGYCGVVRNEG
GKIIREHAGRAYLDPHPTNCVGAWVCPGATSRGYPKYTETRGPERGRYNLAVFYGGCTHHCFFCQNYEHWVERERLSAED
LKRRASDPDVTCVCFFGGDPTPHYDQVIEIGREWEELRVRVCLETNGSLRPKLMRRIAEICYESGGGFNMDVKAYDPRIY
YALSFSDPEYTFKNLEIIGREFAREDPPILRPSTLVVPEYITPEEVEKIAEFLASIDERIPYRLLQHVPQYHSADLGYTP
VCVMRECKRAAERHLENVDACIYRYA

Sequences:

>Translated_346_residues
MTCELCGDPASSFLRVCRECILNRWDKAREILREAHAEARRELGLPPEPPSEGLECGACVNRCRIPEGKVGYCGVVRNEG
GKIIREHAGRAYLDPHPTNCVGAWVCPGATSRGYPKYTETRGPERGRYNLAVFYGGCTHHCFFCQNYEHWVERERLSAED
LKRRASDPDVTCVCFFGGDPTPHYDQVIEIGREWEELRVRVCLETNGSLRPKLMRRIAEICYESGGGFNMDVKAYDPRIY
YALSFSDPEYTFKNLEIIGREFAREDPPILRPSTLVVPEYITPEEVEKIAEFLASIDERIPYRLLQHVPQYHSADLGYTP
VCVMRECKRAAERHLENVDACIYRYA
>Mature_345_residues
TCELCGDPASSFLRVCRECILNRWDKAREILREAHAEARRELGLPPEPPSEGLECGACVNRCRIPEGKVGYCGVVRNEGG
KIIREHAGRAYLDPHPTNCVGAWVCPGATSRGYPKYTETRGPERGRYNLAVFYGGCTHHCFFCQNYEHWVERERLSAEDL
KRRASDPDVTCVCFFGGDPTPHYDQVIEIGREWEELRVRVCLETNGSLRPKLMRRIAEICYESGGGFNMDVKAYDPRIYY
ALSFSDPEYTFKNLEIIGREFAREDPPILRPSTLVVPEYITPEEVEKIAEFLASIDERIPYRLLQHVPQYHSADLGYTPV
CVMRECKRAAERHLENVDACIYRYA

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39629; Mature: 39498

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
5.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCELCGDPASSFLRVCRECILNRWDKAREILREAHAEARRELGLPPEPPSEGLECGACV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
NRCRIPEGKVGYCGVVRNEGGKIIREHAGRAYLDPHPTNCVGAWVCPGATSRGYPKYTET
HHCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
RGPERGRYNLAVFYGGCTHHCFFCQNYEHWVERERLSAEDLKRRASDPDVTCVCFFGGDP
CCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCC
TPHYDQVIEIGREWEELRVRVCLETNGSLRPKLMRRIAEICYESGGGFNMDVKAYDPRIY
CCHHHHHHHHHCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCEEE
YALSFSDPEYTFKNLEIIGREFAREDPPILRPSTLVVPEYITPEEVEKIAEFLASIDERI
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHC
PYRLLQHVPQYHSADLGYTPVCVMRECKRAAERHLENVDACIYRYA
HHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TCELCGDPASSFLRVCRECILNRWDKAREILREAHAEARRELGLPPEPPSEGLECGACV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
NRCRIPEGKVGYCGVVRNEGGKIIREHAGRAYLDPHPTNCVGAWVCPGATSRGYPKYTET
HHCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
RGPERGRYNLAVFYGGCTHHCFFCQNYEHWVERERLSAEDLKRRASDPDVTCVCFFGGDP
CCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCC
TPHYDQVIEIGREWEELRVRVCLETNGSLRPKLMRRIAEICYESGGGFNMDVKAYDPRIY
CCHHHHHHHHHCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCEEE
YALSFSDPEYTFKNLEIIGREFAREDPPILRPSTLVVPEYITPEEVEKIAEFLASIDERI
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHC
PYRLLQHVPQYHSADLGYTPVCVMRECKRAAERHLENVDACIYRYA
HHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA