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The map label for this gene is pcm

Identifier: 20093722

GI number: 20093722

Start: 273412

End: 274092

Strand: Direct

Name: pcm

Synonym: MK0282

Alternate gene names: 20093722

Gene position: 273412-274092 (Clockwise)

Preceding gene: 20093719

Following gene: 20093723

Centisome position: 16.13

GC content: 63.73

Gene sequence:

>681_bases
TTGGCCGAAGACGATAAGGTCTTTCGACGCGCCCGTGAGCGCCTGGTGGAGCGGTTGAAGTCCCTGGGTTACATTAGATC
GAACCGCGTGGCCGAGGCCATGCTCAAGGTGCCGCGTCACGAGTTCGTTCCGGAGGATCTCCGCGATCGGGCCTACGTCG
ACTCGCCCTTACCCATCGGCAGGGGACAGACCATCTCGGCCCCGCACATGGTGGCGATCATGACCGAGTTGCTCGACCCA
CGGCCCGGACACAAGGTGCTCGAGGTGGGGGCGGGTTCGGGGTACCACGCCGCCGTGGTAGCCGAACTGGTGAAACCCGA
CGGTCGTGTGATCACGGTCGAGAGGATCCCGGAGCTCGCGGACTTCGCCCGGAATAACCTGAAGAAAACCGGATACGACC
GTTTCGTGAAGGTGTTGGTGGGGGACGGGACCAAGGGCTACCCGCCCGAAGCCCCGTACGACCGTATTCTGGTGACGGCG
GGCGCCCCGGACGTCCCGGAAAGCCTGCTGGAGCAGCTGAAACCGGGAGGGAAGATGGTCATTCCTGTGGGAGACCGGCA
CATGCAGGAGCTGTGGTTGGTGGAGAAGACCGAGGACGGAGAGATCAGGCGTCGTCGGCACGGTGGATGCGCGTTCGTAC
CGCTCATAGGGGAGGAGGGGTTCCAGGAACCCGAATCTTGA

Upstream 100 bases:

>100_bases
ACGAGACCCAGTCGTATCTCCGACACCTGACACACCCCGACGGCTCCGCGCTCCGACACGTCCAAAACCCCTACGGTCAC
CAGTCGGTGGGGGGACGGTA

Downstream 100 bases:

>100_bases
CCCACTCTAGAGGATATGTACGACCCCGGGGATGAGGAGACCGGCAGGAGGAGACCGAGATGATCGAGGACCCCGATACC
CGACCCGGGGGTGAAGACCA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MAEDDKVFRRARERLVERLKSLGYIRSNRVAEAMLKVPRHEFVPEDLRDRAYVDSPLPIGRGQTISAPHMVAIMTELLDP
RPGHKVLEVGAGSGYHAAVVAELVKPDGRVITVERIPELADFARNNLKKTGYDRFVKVLVGDGTKGYPPEAPYDRILVTA
GAPDVPESLLEQLKPGGKMVIPVGDRHMQELWLVEKTEDGEIRRRRHGGCAFVPLIGEEGFQEPES

Sequences:

>Translated_226_residues
MAEDDKVFRRARERLVERLKSLGYIRSNRVAEAMLKVPRHEFVPEDLRDRAYVDSPLPIGRGQTISAPHMVAIMTELLDP
RPGHKVLEVGAGSGYHAAVVAELVKPDGRVITVERIPELADFARNNLKKTGYDRFVKVLVGDGTKGYPPEAPYDRILVTA
GAPDVPESLLEQLKPGGKMVIPVGDRHMQELWLVEKTEDGEIRRRRHGGCAFVPLIGEEGFQEPES
>Mature_225_residues
AEDDKVFRRARERLVERLKSLGYIRSNRVAEAMLKVPRHEFVPEDLRDRAYVDSPLPIGRGQTISAPHMVAIMTELLDPR
PGHKVLEVGAGSGYHAAVVAELVKPDGRVITVERIPELADFARNNLKKTGYDRFVKVLVGDGTKGYPPEAPYDRILVTAG
APDVPESLLEQLKPGGKMVIPVGDRHMQELWLVEKTEDGEIRRRRHGGCAFVPLIGEEGFQEPES

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=218, Percent_Identity=39.9082568807339, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI190360566, Length=215, Percent_Identity=26.9767441860465, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI157388995, Length=215, Percent_Identity=26.046511627907, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI157388997, Length=185, Percent_Identity=27.027027027027, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789100, Length=178, Percent_Identity=44.3820224719101, Blast_Score=141, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71983477, Length=212, Percent_Identity=33.4905660377358, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI193207222, Length=216, Percent_Identity=31.4814814814815, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17981723, Length=216, Percent_Identity=38.4259259259259, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_METKA (Q8TYL4)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613569.1
- ProteinModelPortal:   Q8TYL4
- SMR:   Q8TYL4
- GeneID:   1477585
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0282
- NMPDR:   fig|190192.1.peg.282
- HOGENOM:   HBG699907
- OMA:   GYHAAVV
- ProtClustDB:   PRK00312
- BioCyc:   MKAN190192:MK0282-MONOMER
- BRENDA:   2.1.1.77
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 25207; Mature: 25076

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 66-66

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDDKVFRRARERLVERLKSLGYIRSNRVAEAMLKVPRHEFVPEDLRDRAYVDSPLPIG
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCCCCCC
RGQTISAPHMVAIMTELLDPRPGHKVLEVGAGSGYHAAVVAELVKPDGRVITVERIPELA
CCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEHHHCHHHH
DFARNNLKKTGYDRFVKVLVGDGTKGYPPEAPYDRILVTAGAPDVPESLLEQLKPGGKMV
HHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEE
IPVGDRHMQELWLVEKTEDGEIRRRRHGGCAFVPLIGEEGFQEPES
EECCCHHHHHHHHEECCCCCHHHHHHCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
AEDDKVFRRARERLVERLKSLGYIRSNRVAEAMLKVPRHEFVPEDLRDRAYVDSPLPIG
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCCCCCC
RGQTISAPHMVAIMTELLDPRPGHKVLEVGAGSGYHAAVVAELVKPDGRVITVERIPELA
CCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEHHHCHHHH
DFARNNLKKTGYDRFVKVLVGDGTKGYPPEAPYDRILVTAGAPDVPESLLEQLKPGGKMV
HHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEE
IPVGDRHMQELWLVEKTEDGEIRRRRHGGCAFVPLIGEEGFQEPES
EECCCHHHHHHHHEECCCCCHHHHHHCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014