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The map label for this gene is ribH

Identifier: 20093721

GI number: 20093721

Start: 272912

End: 273337

Strand: Reverse

Name: ribH

Synonym: MK0281

Alternate gene names: 20093721

Gene position: 273337-272912 (Counterclockwise)

Preceding gene: 20093724

Following gene: 20093720

Centisome position: 16.13

GC content: 62.21

Gene sequence:

>426_bases
GTGTCGGAGATACGACTGGGTCTCGTCGTGACGGAATTCAACCGGGAGATAACCTACGCGATGGAGGAGCTCGCGGTCCA
ACACGCCAAGGATCTTGGGGCGAAGGTCGTCGAACGCGTCCTCGTCCCCGGATCCTTCGAGGTACCGTTGGCGGTCAGGA
AGCTCCTCGAGCGCGACGACATCGACGCCGTCGTGACCCTAGGTGCGATCATCAAGGGTGACACCGACCACGACCAGGCT
ATCGCGCAACAGGCGTTCAGGAAGATACAGGATCTGATGGTCGAGTACGGTAAGCCCGTGGCGTTAGGTATCTCCGGTCC
GGGCATGACCAGGATGGAGGCCCTGGAGCGCGTTCACTACGCCAAGCGCGCCGTAGAAGCCGCCGTCAAGATGGTCCGGC
GGCTGCGGGAGCTGGAGGGTGAATAG

Upstream 100 bases:

>100_bases
CGAAAGACCTTATCGTCTTCGGCCAATACCGTCCCCCCACCGACTGGTGACCGTAGGGGTTTTGGACGTGTCGGAGCGCG
GAGCCGTCGGGGTGTGTCAG

Downstream 100 bases:

>100_bases
CGACCCGTCCGGTCGGCCGGAGGTGGTGCGCCGTCCCTCGTCCCGTCCCCGGAGACGCGGAGTTGGACGAGTGCCGCTCC
TACCCCCAGCACCTTCCGCC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MSEIRLGLVVTEFNREITYAMEELAVQHAKDLGAKVVERVLVPGSFEVPLAVRKLLERDDIDAVVTLGAIIKGDTDHDQA
IAQQAFRKIQDLMVEYGKPVALGISGPGMTRMEALERVHYAKRAVEAAVKMVRRLRELEGE

Sequences:

>Translated_141_residues
MSEIRLGLVVTEFNREITYAMEELAVQHAKDLGAKVVERVLVPGSFEVPLAVRKLLERDDIDAVVTLGAIIKGDTDHDQA
IAQQAFRKIQDLMVEYGKPVALGISGPGMTRMEALERVHYAKRAVEAAVKMVRRLRELEGE
>Mature_140_residues
SEIRLGLVVTEFNREITYAMEELAVQHAKDLGAKVVERVLVPGSFEVPLAVRKLLERDDIDAVVTLGAIIKGDTDHDQAI
AQQAFRKIQDLMVEYGKPVALGISGPGMTRMEALERVHYAKRAVEAAVKMVRRLRELEGE

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_METKA (Q8TYL5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613568.1
- ProteinModelPortal:   Q8TYL5
- SMR:   Q8TYL5
- GeneID:   1477584
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0281
- NMPDR:   fig|190192.1.peg.281
- HOGENOM:   HBG311126
- OMA:   AVIQGAT
- ProtClustDB:   PRK00061
- BioCyc:   MKAN190192:MK0281-MONOMER
- BRENDA:   2.5.1.9
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 15698; Mature: 15566

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIRLGLVVTEFNREITYAMEELAVQHAKDLGAKVVERVLVPGSFEVPLAVRKLLERDD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
IDAVVTLGAIIKGDTDHDQAIAQQAFRKIQDLMVEYGKPVALGISGPGMTRMEALERVHY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
AKRAVEAAVKMVRRLRELEGE
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEIRLGLVVTEFNREITYAMEELAVQHAKDLGAKVVERVLVPGSFEVPLAVRKLLERDD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
IDAVVTLGAIIKGDTDHDQAIAQQAFRKIQDLMVEYGKPVALGISGPGMTRMEALERVHY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
AKRAVEAAVKMVRRLRELEGE
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014