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The map label for this gene is pheS

Identifier: 20093711

GI number: 20093711

Start: 260022

End: 261596

Strand: Direct

Name: pheS

Synonym: MK0271

Alternate gene names: 20093711

Gene position: 260022-261596 (Clockwise)

Preceding gene: 20093709

Following gene: 20093713

Centisome position: 15.34

GC content: 60.51

Gene sequence:

>1575_bases
GTGGACCCGCGGGAACTGGCTGAGCGGTTGACCGAGCGCGACTTAAGGATCCTCATTCACTTAGCGGAAAGCGGGGAAGC
AACACCCGAGGAACTCGCCGAGTCGCTGGACGTCGACCTGGGTCCCGTTATGAGATCACTCTACTGGCTCGAGGAGCGTG
GGTTGATAGAGTCCGAGGAAGAAACGCACGAGGTGTACGAGTTAGGTGATGAAGGAAAGGAGTACGCCGAGGAAGGGCTG
CCGGAGCTCCGCATCGTCGAGGTCTTAAGAAAGATAGGCGGTGAGGGACGACTGGAAGAGGTCCTAGATCGTGCCGGAGT
GCCCCGGAAGCTGGCCGGACCCGTCCTAGGATGGCTGAGGAGGAAAGGACTGGCGGAGATCAAGAGGGAGGACGGGGAGA
CGTCGCTCGTTCTCCTGGAGGAGGAGCCTGAGGACGTGGATCAATCCGTGCTCGAGGCGTTGGCCGCTGAGGGTTCCGCG
TCCGTCGAGGAGCTTGCGAGAAAGCTAGAGATGGATGAAGAGGAAGTGGAGAAGGCCCTGAAGAGGTTGAGTGAGCGCGG
TGACGTCCTGAGAGCGCGGGAGGAGACCGTCAAGAAAGTCAGGCTAACCGAACGCGGTGAGGAAGTCGCGGAGCACGCCC
CAGAAGTGCTGGAACGTGACTGGATCACGGAGCTGAAACCCGAGCATTTGAGGGAGGGAACTTGGAAGGAGAAGGAGTTC
AAGCCATACGACGTGAAGGCACCCACGAGTCCTACCTTCCCCGGCAAGCGCCACCCACTGAAGGAGGTTATCAACGAGAT
ACGTAGGATCTTCCTCGAGATGGGTTTCGTGGAGGTGTCCGGACCGCTGGTCGAGTCCAGCTTCTGGAACTTCGACGCCC
TGTTCCAGCCGCAGGATCACGCCGCCAGGGAGATGCAGGACACCTTCTACCTGAAAGAACCCGCCGAGGCCGAATTGCCG
GACGAGGAAGTCGTCGAGAAGGTCCGGGCCGTGCACGAGGATGGTGGCGACACGGGATCCAGGGGTTGGGGGTACGAGTG
GGACGAGGGTGTGGCCCGAAAGACGGTCCTTCGTACTCACACGACGGCGGTGTCGGTTCGGAAACTGTACGAGGTGGAGG
GGCCTCCACTCAAAGCGTTCTCGATCGGCCGGGTGTACCGGCGCGAGACGGTCGACTACAAGCACTTGCCGGAGTTCCAT
CAGTGTGAGGGAATAGTCCTGGCCAAGGACGTCTCGTTCCGGGACTTGCTCGGGATACTCGAGGAGTTCTACCGACGGAT
GGGGTTCGAAGAGGTCAGGTTCAGGCCCGCCTACTTCCCCTATACGGTCCTCTCCGTCGAACCCGAGGTATACTTCGAGG
AGAAAGGCGATTGGGTGGAGCTAGGCGGTGCGGGCATTTTCCGCCCTGAGGTGCTTCAACCCCTGGGGTTCGATCCCGAC
GTGGTGTGTTTAGCATGGGGCTTGGGCGTCGAACGACTGGCGATGCTGAAGTTGGGAATAGACGACATACGAGATCTGTA
CATGAGCGACCTCAAGACGCTCCTGGAGCTACCCACCGCCAGGGCACGGCGCTGA

Upstream 100 bases:

>100_bases
AGGTCCCCCGAATGGGGGCGACCGTGATCCCGGGCGGGTTTACCCCGCCCCAATCCGCCACGCAGGGTCGCAGCGGCCGA
ACGAACCGGGGGTCCGCCGC

Downstream 100 bases:

>100_bases
TCAGCGGTCCAGGCGTCTCCACACGTCCCTCCAGCGATCCCCACACCTGTGCTTAACCTCGTACTTCAGCAGGTTAAGCA
GCGTGGCCACGAATACCTCG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MDPRELAERLTERDLRILIHLAESGEATPEELAESLDVDLGPVMRSLYWLEERGLIESEEETHEVYELGDEGKEYAEEGL
PELRIVEVLRKIGGEGRLEEVLDRAGVPRKLAGPVLGWLRRKGLAEIKREDGETSLVLLEEEPEDVDQSVLEALAAEGSA
SVEELARKLEMDEEEVEKALKRLSERGDVLRAREETVKKVRLTERGEEVAEHAPEVLERDWITELKPEHLREGTWKEKEF
KPYDVKAPTSPTFPGKRHPLKEVINEIRRIFLEMGFVEVSGPLVESSFWNFDALFQPQDHAAREMQDTFYLKEPAEAELP
DEEVVEKVRAVHEDGGDTGSRGWGYEWDEGVARKTVLRTHTTAVSVRKLYEVEGPPLKAFSIGRVYRRETVDYKHLPEFH
QCEGIVLAKDVSFRDLLGILEEFYRRMGFEEVRFRPAYFPYTVLSVEPEVYFEEKGDWVELGGAGIFRPEVLQPLGFDPD
VVCLAWGLGVERLAMLKLGIDDIRDLYMSDLKTLLELPTARARR

Sequences:

>Translated_524_residues
MDPRELAERLTERDLRILIHLAESGEATPEELAESLDVDLGPVMRSLYWLEERGLIESEEETHEVYELGDEGKEYAEEGL
PELRIVEVLRKIGGEGRLEEVLDRAGVPRKLAGPVLGWLRRKGLAEIKREDGETSLVLLEEEPEDVDQSVLEALAAEGSA
SVEELARKLEMDEEEVEKALKRLSERGDVLRAREETVKKVRLTERGEEVAEHAPEVLERDWITELKPEHLREGTWKEKEF
KPYDVKAPTSPTFPGKRHPLKEVINEIRRIFLEMGFVEVSGPLVESSFWNFDALFQPQDHAAREMQDTFYLKEPAEAELP
DEEVVEKVRAVHEDGGDTGSRGWGYEWDEGVARKTVLRTHTTAVSVRKLYEVEGPPLKAFSIGRVYRRETVDYKHLPEFH
QCEGIVLAKDVSFRDLLGILEEFYRRMGFEEVRFRPAYFPYTVLSVEPEVYFEEKGDWVELGGAGIFRPEVLQPLGFDPD
VVCLAWGLGVERLAMLKLGIDDIRDLYMSDLKTLLELPTARARR
>Mature_524_residues
MDPRELAERLTERDLRILIHLAESGEATPEELAESLDVDLGPVMRSLYWLEERGLIESEEETHEVYELGDEGKEYAEEGL
PELRIVEVLRKIGGEGRLEEVLDRAGVPRKLAGPVLGWLRRKGLAEIKREDGETSLVLLEEEPEDVDQSVLEALAAEGSA
SVEELARKLEMDEEEVEKALKRLSERGDVLRAREETVKKVRLTERGEEVAEHAPEVLERDWITELKPEHLREGTWKEKEF
KPYDVKAPTSPTFPGKRHPLKEVINEIRRIFLEMGFVEVSGPLVESSFWNFDALFQPQDHAAREMQDTFYLKEPAEAELP
DEEVVEKVRAVHEDGGDTGSRGWGYEWDEGVARKTVLRTHTTAVSVRKLYEVEGPPLKAFSIGRVYRRETVDYKHLPEFH
QCEGIVLAKDVSFRDLLGILEEFYRRMGFEEVRFRPAYFPYTVLSVEPEVYFEEKGDWVELGGAGIFRPEVLQPLGFDPD
VVCLAWGLGVERLAMLKLGIDDIRDLYMSDLKTLLELPTARARR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=527, Percent_Identity=36.2428842504744, Blast_Score=285, Evalue=8e-77,
Organism=Escherichia coli, GI1788007, Length=276, Percent_Identity=34.7826086956522, Blast_Score=162, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI32563657, Length=513, Percent_Identity=36.6471734892787, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17508957, Length=513, Percent_Identity=36.6471734892787, Blast_Score=296, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6321087, Length=472, Percent_Identity=33.8983050847458, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6325304, Length=81, Percent_Identity=44.4444444444444, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI18858079, Length=531, Percent_Identity=35.969868173258, Blast_Score=270, Evalue=2e-72,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_METKA (Q8TYM5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613558.1
- ProteinModelPortal:   Q8TYM5
- SMR:   Q8TYM5
- GeneID:   1477574
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0271
- NMPDR:   fig|190192.1.peg.271
- HOGENOM:   HBG497668
- OMA:   PEFYQCE
- ProtClustDB:   PRK04172
- BioCyc:   MKAN190192:MK0271-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00282
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319
- InterPro:   IPR002831
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF01978 TrmB; PF01409 tRNA-synt_2d

EC number: =6.1.1.20

Molecular weight: Translated: 60079; Mature: 60079

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPRELAERLTERDLRILIHLAESGEATPEELAESLDVDLGPVMRSLYWLEERGLIESEE
CCHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHH
ETHEVYELGDEGKEYAEEGLPELRIVEVLRKIGGEGRLEEVLDRAGVPRKLAGPVLGWLR
HHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHH
RKGLAEIKREDGETSLVLLEEEPEDVDQSVLEALAAEGSASVEELARKLEMDEEEVEKAL
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHH
KRLSERGDVLRAREETVKKVRLTERGEEVAEHAPEVLERDWITELKPEHLREGTWKEKEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCC
KPYDVKAPTSPTFPGKRHPLKEVINEIRRIFLEMGFVEVSGPLVESSFWNFDALFQPQDH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCHHHCCCCCCCCCCCCCHH
AAREMQDTFYLKEPAEAELPDEEVVEKVRAVHEDGGDTGSRGWGYEWDEGVARKTVLRTH
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
TTAVSVRKLYEVEGPPLKAFSIGRVYRRETVDYKHLPEFHQCEGIVLAKDVSFRDLLGIL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHCCCHHHCCCEEEECCCCHHHHHHHH
EEFYRRMGFEEVRFRPAYFPYTVLSVEPEVYFEEKGDWVELGGAGIFRPEVLQPLGFDPD
HHHHHHCCHHHHCCCCCCCCEEEEEECCCEEECCCCCEEEECCCCCCCHHHHCCCCCCCC
VVCLAWGLGVERLAMLKLGIDDIRDLYMSDLKTLLELPTARARR
HHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MDPRELAERLTERDLRILIHLAESGEATPEELAESLDVDLGPVMRSLYWLEERGLIESEE
CCHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHH
ETHEVYELGDEGKEYAEEGLPELRIVEVLRKIGGEGRLEEVLDRAGVPRKLAGPVLGWLR
HHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHH
RKGLAEIKREDGETSLVLLEEEPEDVDQSVLEALAAEGSASVEELARKLEMDEEEVEKAL
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHH
KRLSERGDVLRAREETVKKVRLTERGEEVAEHAPEVLERDWITELKPEHLREGTWKEKEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCC
KPYDVKAPTSPTFPGKRHPLKEVINEIRRIFLEMGFVEVSGPLVESSFWNFDALFQPQDH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCHHHCCCCCCCCCCCCCHH
AAREMQDTFYLKEPAEAELPDEEVVEKVRAVHEDGGDTGSRGWGYEWDEGVARKTVLRTH
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
TTAVSVRKLYEVEGPPLKAFSIGRVYRRETVDYKHLPEFHQCEGIVLAKDVSFRDLLGIL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHCCCHHHCCCEEEECCCCHHHHHHHH
EEFYRRMGFEEVRFRPAYFPYTVLSVEPEVYFEEKGDWVELGGAGIFRPEVLQPLGFDPD
HHHHHHCCHHHHCCCCCCCCEEEEEECCCEEECCCCCEEEECCCCCCCHHHHCCCCCCCC
VVCLAWGLGVERLAMLKLGIDDIRDLYMSDLKTLLELPTARARR
HHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014