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The map label for this gene is PpsA [H]

Identifier: 20093692

GI number: 20093692

Start: 242469

End: 244781

Strand: Direct

Name: PpsA [H]

Synonym: MK0252

Alternate gene names: 20093692

Gene position: 242469-244781 (Clockwise)

Preceding gene: 20093691

Following gene: 20093693

Centisome position: 14.31

GC content: 60.61

Gene sequence:

>2313_bases
GTGAAGTACGTCCGGTGGTTCGAGGAGATATCGAAGGATGACGTCGACGTCGCCGGAGGCAAGGGCGCGAACCTGGGCGA
GATGACGCAGGCGGGACTACCAGTCCCGCCCGGCTTCGTGGTCCTGTCCACAGCCTACGATGAGTTCCTCGAGCGCACTG
GCCTGAAGGAGAAGATAAAGGAAATACTTTCCTCCCACGACCTGTCCGACAACGACGAGCTTCAGGAGGCCGCGGAGGAG
ATTCAGCGGCTGATCGTTGAGGTCGAAATGCCGGAGGAGATCCGTGAGGAGATCGTGAAAGCCTACCGGGAGCTCTGCGA
GAAGGTCGGTAAGGAAGAGGAGTTCGTAGCTGTCCGCTCGTCGGCGACCGCCGAAGACTTACCGGAAGCTTCCTTCGCGG
GCCAGCAGGAGACGTTCCTGAACGTCCAAGGTGAGGAGGACGTCGTGAAGTACGTGCAGAAGTGTTGGGCGTCCCTATTC
ACCCCTCGGGCCGTGGCTTACCGGGAGGAGCAAGGGTTCGAGCATCTGGACGTTTCGATCGCCGTCGTCGTCCAGAAGAT
GGTAGATTCGGAGAAGTCAGGAGTAATGTTCACGGTCCACCCCTACACCGGCGAGCGGGACAAGATGGTGATCGAAGCCG
TGTGGGGTCTCGGCGAGGCCGTAGTCAGTGGGGAAGTCACTCCCGACACCTACATCGTCGACAAGAACACGTTCGAGGTC
ATCGAGGAGCAGATATCCGAGCAGGAGTGGATGTACACGAAGGACCCGGAAACAGGGGAGACGGTCAAGGCCGAGGTGCC
TGAGGATAAACGCGACGCTAGGAAGCTGACGGACGAAGAGATCAAGGAACTGGCGGAGATCGGAGTAGCCGTGGAGGAGC
ACTACGGGTTCCCGCAGGATATCGAGTGGGCCATCGAGGACGACGAAGTATACGTGCTGCAGTCGCGTCCGGTCACCACA
ATACCAGAGGAGAAGGGCGGAGAGGAAGAGATAGAGGCGGAAGAGTTGGAGGGCAAGATCCTCGTCCGCGGCCTAGGGGC
CAGCCCGGGGATCGGTACGGGCGAAGTCAAGATCGTGATGGACGTGGACGAGATCGACAAGGTGGAGGAAGGGGACGTCC
TGGTCACCAAGATGACCACACCGGACATGGTCCCGGCGATGCGGAAGGCGTCGGCCATAGTCACCGACGAAGGCGGAATC
ACATGCCACGCCGCGATCGTGTCGCGAGAGCTGGGAATCCCGTGTGTGGTCGGTACGGGCAACGCCACGGAGGTCCTCGA
GGAGGGGCAGGTGGTAACGGTCGACGGAGAGCGTGGAGTCGTGTACGAGGGTGACGTGCGCAAGGCCCTCCGTGCCGAGG
AGGAAGAGGAGGAGCGGGAAGAGAAGGAGATCGTGGTGGAGCGCCCGGCCGCTGAGCCAGTGACCGCTACGGAGATCAAG
GTTAACGTCAGCATGCCCGAAGCCGCGGAGCGTGCCGCTAAGACGGGAGCCGACGGTGTCGGATTGCTCCGTATCGAACA
CATGATCCTGGGCGTGGGAGTACATCCCCGCAAGCTCATCGAGGAGGGTGAGCGCGAGAAACTCGTGCAAGTGTTGATGG
ACGGGATCCGCAAGGTCGCGGACGCCTTCTACCCGAAGCCCGTATGGGTCAGAACCCTGGACGCTCCGACCGACGAGTTC
CGCGAGCTCGAAGGTGGTGAGCGGGAGCCCGAAGAAACCAACCCGATGCTCGGTTGGAGAGGTATTCGTCGCGATCTCGA
GGAGCGGGAGACTCTCGAGTGTCAGTTCGAGGCTATCCGCCGCCTCCACCAGGAGGGTTACGATAACATCGGCGTGATGA
TTCCGTTGGTACAGCACCCCGAAGAACTGCGCCGCGCTAAGCGGATCGCCAAGGAAGTAGGGCTCAAACCGCATCGTCAG
GTCGAGTTCGGAATGATGGTGGAAACACCTGCCGCCGCGGTACTAATCGACGAATTCATCGAAGTAGGCCTCGACTTCGT
GAGCCTCGGTACCAACGACCTCACCCAGTACACGTTGGCGGTCGACAGGAACAACGACAAGGTGGCATACCTCTACGACG
AGAAGCACCCGGCAGTACTCCGGCTGATCAAACACGTCATCAACGAGTGCAAGGAGGCTGGAGTGAAGACGAGCATCTGC
GGTCAGGCGGGTAGCGACCCGAAGATGGCCGAGATACTAGTGAAAGCGGGCATCGACAGCATCTCGGCGAACATCGACGC
GGTGCCCCAGATCCGGCGGATCGTAGCCCGGGTAGAGCGGAAGATACTGCTGGATAAGATGAGGGAGTTCTAA

Upstream 100 bases:

>100_bases
GGGCATGAAGTTCCCGTTCCCGTTCACCATAGTGGTCGACAAGGGTGAAGAACTGGTTCAGGACTGATGCGATACCGATC
CGTGCTGAGGGGGGCCCACC

Downstream 100 bases:

>100_bases
CGCCCGTGTCCCTCCTGAAACATCTTTCCGAGCTGGGATTGTCCGAACGAGAGGCGCGGGTGTACCTGTACCTGGTCCGC
GCCGGTGAGGCCAACGCACG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 770; Mature: 770

Protein sequence:

>770_residues
MKYVRWFEEISKDDVDVAGGKGANLGEMTQAGLPVPPGFVVLSTAYDEFLERTGLKEKIKEILSSHDLSDNDELQEAAEE
IQRLIVEVEMPEEIREEIVKAYRELCEKVGKEEEFVAVRSSATAEDLPEASFAGQQETFLNVQGEEDVVKYVQKCWASLF
TPRAVAYREEQGFEHLDVSIAVVVQKMVDSEKSGVMFTVHPYTGERDKMVIEAVWGLGEAVVSGEVTPDTYIVDKNTFEV
IEEQISEQEWMYTKDPETGETVKAEVPEDKRDARKLTDEEIKELAEIGVAVEEHYGFPQDIEWAIEDDEVYVLQSRPVTT
IPEEKGGEEEIEAEELEGKILVRGLGASPGIGTGEVKIVMDVDEIDKVEEGDVLVTKMTTPDMVPAMRKASAIVTDEGGI
TCHAAIVSRELGIPCVVGTGNATEVLEEGQVVTVDGERGVVYEGDVRKALRAEEEEEEREEKEIVVERPAAEPVTATEIK
VNVSMPEAAERAAKTGADGVGLLRIEHMILGVGVHPRKLIEEGEREKLVQVLMDGIRKVADAFYPKPVWVRTLDAPTDEF
RELEGGEREPEETNPMLGWRGIRRDLEERETLECQFEAIRRLHQEGYDNIGVMIPLVQHPEELRRAKRIAKEVGLKPHRQ
VEFGMMVETPAAAVLIDEFIEVGLDFVSLGTNDLTQYTLAVDRNNDKVAYLYDEKHPAVLRLIKHVINECKEAGVKTSIC
GQAGSDPKMAEILVKAGIDSISANIDAVPQIRRIVARVERKILLDKMREF

Sequences:

>Translated_770_residues
MKYVRWFEEISKDDVDVAGGKGANLGEMTQAGLPVPPGFVVLSTAYDEFLERTGLKEKIKEILSSHDLSDNDELQEAAEE
IQRLIVEVEMPEEIREEIVKAYRELCEKVGKEEEFVAVRSSATAEDLPEASFAGQQETFLNVQGEEDVVKYVQKCWASLF
TPRAVAYREEQGFEHLDVSIAVVVQKMVDSEKSGVMFTVHPYTGERDKMVIEAVWGLGEAVVSGEVTPDTYIVDKNTFEV
IEEQISEQEWMYTKDPETGETVKAEVPEDKRDARKLTDEEIKELAEIGVAVEEHYGFPQDIEWAIEDDEVYVLQSRPVTT
IPEEKGGEEEIEAEELEGKILVRGLGASPGIGTGEVKIVMDVDEIDKVEEGDVLVTKMTTPDMVPAMRKASAIVTDEGGI
TCHAAIVSRELGIPCVVGTGNATEVLEEGQVVTVDGERGVVYEGDVRKALRAEEEEEEREEKEIVVERPAAEPVTATEIK
VNVSMPEAAERAAKTGADGVGLLRIEHMILGVGVHPRKLIEEGEREKLVQVLMDGIRKVADAFYPKPVWVRTLDAPTDEF
RELEGGEREPEETNPMLGWRGIRRDLEERETLECQFEAIRRLHQEGYDNIGVMIPLVQHPEELRRAKRIAKEVGLKPHRQ
VEFGMMVETPAAAVLIDEFIEVGLDFVSLGTNDLTQYTLAVDRNNDKVAYLYDEKHPAVLRLIKHVINECKEAGVKTSIC
GQAGSDPKMAEILVKAGIDSISANIDAVPQIRRIVARVERKILLDKMREF
>Mature_770_residues
MKYVRWFEEISKDDVDVAGGKGANLGEMTQAGLPVPPGFVVLSTAYDEFLERTGLKEKIKEILSSHDLSDNDELQEAAEE
IQRLIVEVEMPEEIREEIVKAYRELCEKVGKEEEFVAVRSSATAEDLPEASFAGQQETFLNVQGEEDVVKYVQKCWASLF
TPRAVAYREEQGFEHLDVSIAVVVQKMVDSEKSGVMFTVHPYTGERDKMVIEAVWGLGEAVVSGEVTPDTYIVDKNTFEV
IEEQISEQEWMYTKDPETGETVKAEVPEDKRDARKLTDEEIKELAEIGVAVEEHYGFPQDIEWAIEDDEVYVLQSRPVTT
IPEEKGGEEEIEAEELEGKILVRGLGASPGIGTGEVKIVMDVDEIDKVEEGDVLVTKMTTPDMVPAMRKASAIVTDEGGI
TCHAAIVSRELGIPCVVGTGNATEVLEEGQVVTVDGERGVVYEGDVRKALRAEEEEEEREEKEIVVERPAAEPVTATEIK
VNVSMPEAAERAAKTGADGVGLLRIEHMILGVGVHPRKLIEEGEREKLVQVLMDGIRKVADAFYPKPVWVRTLDAPTDEF
RELEGGEREPEETNPMLGWRGIRRDLEERETLECQFEAIRRLHQEGYDNIGVMIPLVQHPEELRRAKRIAKEVGLKPHRQ
VEFGMMVETPAAAVLIDEFIEVGLDFVSLGTNDLTQYTLAVDRNNDKVAYLYDEKHPAVLRLIKHVINECKEAGVKTSIC
GQAGSDPKMAEILVKAGIDSISANIDAVPQIRRIVARVERKILLDKMREF

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=807, Percent_Identity=39.1573729863693, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1788756, Length=406, Percent_Identity=32.512315270936, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789193, Length=400, Percent_Identity=31.5, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI48994992, Length=399, Percent_Identity=28.0701754385965, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788726, Length=406, Percent_Identity=26.3546798029557, Blast_Score=130, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17564524, Length=330, Percent_Identity=32.1212121212121, Blast_Score=136, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 85981; Mature: 85981

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVRWFEEISKDDVDVAGGKGANLGEMTQAGLPVPPGFVVLSTAYDEFLERTGLKEKIK
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEHHHHHHHHHHCCHHHHHH
EILSSHDLSDNDELQEAAEEIQRLIVEVEMPEEIREEIVKAYRELCEKVGKEEEFVAVRS
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
SATAEDLPEASFAGQQETFLNVQGEEDVVKYVQKCWASLFTPRAVAYREEQGFEHLDVSI
CCCHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHH
AVVVQKMVDSEKSGVMFTVHPYTGERDKMVIEAVWGLGEAVVSGEVTPDTYIVDKNTFEV
HHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHCCCCCCCEEEECCCHHHH
IEEQISEQEWMYTKDPETGETVKAEVPEDKRDARKLTDEEIKELAEIGVAVEEHYGFPQD
HHHHHHHCCCEEECCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCC
IEWAIEDDEVYVLQSRPVTTIPEEKGGEEEIEAEELEGKILVRGLGASPGIGTGEVKIVM
CCEEEECCCEEEECCCCCCCCCCCCCCCHHCCHHHCCCEEEEEECCCCCCCCCCCEEEEE
DVDEIDKVEEGDVLVTKMTTPDMVPAMRKASAIVTDEGGITCHAAIVSRELGIPCVVGTG
EHHHHCCCCCCCEEEEECCCCHHHHHHHHCCEEEECCCCCEEEHHHHHHCCCCEEEEECC
NATEVLEEGQVVTVDGERGVVYEGDVRKALRAEEEEEEREEKEIVVERPAAEPVTATEIK
CHHHHHHCCCEEEEECCCCEEEECHHHHHHHCCHHHHHHHHHHEEEECCCCCCCEEEEEE
VNVSMPEAAERAAKTGADGVGLLRIEHMILGVGVHPRKLIEEGEREKLVQVLMDGIRKVA
EEECCCHHHHHHHHCCCCCCHHHEEHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
DAFYPKPVWVRTLDAPTDEFRELEGGEREPEETNPMLGWRGIRRDLEERETLECQFEAIR
HHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLHQEGYDNIGVMIPLVQHPEELRRAKRIAKEVGLKPHRQVEFGMMVETPAAAVLIDEFI
HHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEECEEECCCHHHHHHHHHH
EVGLDFVSLGTNDLTQYTLAVDRNNDKVAYLYDEKHPAVLRLIKHVINECKEAGVKTSIC
HHCCHHEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEC
GQAGSDPKMAEILVKAGIDSISANIDAVPQIRRIVARVERKILLDKMREF
CCCCCCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYVRWFEEISKDDVDVAGGKGANLGEMTQAGLPVPPGFVVLSTAYDEFLERTGLKEKIK
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEHHHHHHHHHHCCHHHHHH
EILSSHDLSDNDELQEAAEEIQRLIVEVEMPEEIREEIVKAYRELCEKVGKEEEFVAVRS
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
SATAEDLPEASFAGQQETFLNVQGEEDVVKYVQKCWASLFTPRAVAYREEQGFEHLDVSI
CCCHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHH
AVVVQKMVDSEKSGVMFTVHPYTGERDKMVIEAVWGLGEAVVSGEVTPDTYIVDKNTFEV
HHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHCCCCCCCEEEECCCHHHH
IEEQISEQEWMYTKDPETGETVKAEVPEDKRDARKLTDEEIKELAEIGVAVEEHYGFPQD
HHHHHHHCCCEEECCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCC
IEWAIEDDEVYVLQSRPVTTIPEEKGGEEEIEAEELEGKILVRGLGASPGIGTGEVKIVM
CCEEEECCCEEEECCCCCCCCCCCCCCCHHCCHHHCCCEEEEEECCCCCCCCCCCEEEEE
DVDEIDKVEEGDVLVTKMTTPDMVPAMRKASAIVTDEGGITCHAAIVSRELGIPCVVGTG
EHHHHCCCCCCCEEEEECCCCHHHHHHHHCCEEEECCCCCEEEHHHHHHCCCCEEEEECC
NATEVLEEGQVVTVDGERGVVYEGDVRKALRAEEEEEEREEKEIVVERPAAEPVTATEIK
CHHHHHHCCCEEEEECCCCEEEECHHHHHHHCCHHHHHHHHHHEEEECCCCCCCEEEEEE
VNVSMPEAAERAAKTGADGVGLLRIEHMILGVGVHPRKLIEEGEREKLVQVLMDGIRKVA
EEECCCHHHHHHHHCCCCCCHHHEEHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
DAFYPKPVWVRTLDAPTDEFRELEGGEREPEETNPMLGWRGIRRDLEERETLECQFEAIR
HHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLHQEGYDNIGVMIPLVQHPEELRRAKRIAKEVGLKPHRQVEFGMMVETPAAAVLIDEFI
HHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEECEEECCCHHHHHHHHHH
EVGLDFVSLGTNDLTQYTLAVDRNNDKVAYLYDEKHPAVLRLIKHVINECKEAGVKTSIC
HHCCHHEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEC
GQAGSDPKMAEILVKAGIDSISANIDAVPQIRRIVARVERKILLDKMREF
CCCCCCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]