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The map label for this gene is pyrG

Identifier: 20093645

GI number: 20093645

Start: 197414

End: 199021

Strand: Reverse

Name: pyrG

Synonym: MK0205

Alternate gene names: 20093645

Gene position: 199021-197414 (Counterclockwise)

Preceding gene: 20093647

Following gene: 20093642

Centisome position: 11.74

GC content: 60.45

Gene sequence:

>1608_bases
GTGAAGTTCGTCGTGATCACGGGTGGGGTAGTGTCCGGTATCGGGAAAGGTATCACCACGGCGTCTATCGGTAGGATCCT
AAGGGCACGAGAGCTGGAAGTTACCGCGGTCAAGATCGACCCTTACATTAACGTGGACGCGGGCACCATGAACCCTTTTC
AACATGGAGAGGTGTTCGTCACGGAGGACGGTGTTGAGACGGATCTGGACCTGGGCCATTATGAACGGTTCATGGACGTC
ACGCTATCCGGAGCCCACAATATCACGACTGGCAAGATCTACCAGCGGGTGATCGAAAAGGAGCGCCGTGGGGACTACTT
GGGCGAGACCGTCCAGGTGATTCCCCACATAACCGACGAGATCAAGTCGTGGATCCGCGAGGTGGGCAAGGCCAGCGGTG
CGGACGTCGTGCTGGTGGAGATAGGCGGTACCGTCGGCGACATTGAAGGGATGCCCTTCTACGAGGCGGTTCGGCAGTTG
CAGCTGGAGGAAGGTCGAGAGAACGTGATGTTCGTCCACCTGACCTACGTCCCATACTTGGAACACGTCCACGAGCTGAA
GACGAAGCCCACCCAACATAGCGTCAAGGAGCTCCGTAGTCTAGGTATCCAGCCGGACGCCATCGTGTGCAGGTGTGAAC
GTCCCCTCGACGACGGCGTCAAACGGAAGATCGCCCTGCATACGAACGTCCCGAGGGAGGCCGTAATCGACGCCCACGAC
GTCGATCTGGTGTACAAGGTGCCGTTGTTGCTCGAGCGTCAGGGCTTCGGCGATTACATCTGCGAGCGTCTGGGTCTCGA
CGCGGACGAGCCCGATTACTCCGACTGGCTGGATTTCGTTACCAGGATCGAGGAAGCCGACGACGAGATCAGGATCGCGG
TCGTGGGTAAGTACGTGGACCTACCGGACGCCTACATCAGCATCCGGGAGGCCTTAGTCCACGCCGGGGCTCATGTCGGG
GTTGGCGTGGACGTAGCGTGGGTGGACTCGGAGGCGCTCGAAGCCGGCGACTCCGAAGCGTGGGAGGAGGTGAAAGACGC
CGACGGTGTACTAGTTCCAGGTGGCTTCGGCAAGAGAGGTGTCGAAGGTAAGATCGAGGCGGTTCGATACGCCCGGGAGA
ACGACGTTCCGTTCCTGGGTATCTGCTTGGGCTTCCAGCTCGTCGTGGTCGAGTACGCCCGCAGCGTTCTCGGGCTCGAG
GACGCTCACAGCACGGAGTTCAATCCCGACACCGATCATCCCGTCGTGGACTTGCTCCCGGAACAGCGCGGAGTGAAGCG
GAAAGGAGGCACGATGCGGTTAGGCGCCGAGCCCGTGGTGCTCGAGGAAGGATCGCTCCTCAGACGCCTCTACGACGACC
GTGAGATCGTTTTGGAACGGCATCGACACCGTTACGAAGTGAACCCGTCGTACGTTCGCGAACTGGAGGACCATGGACTG
AGGTTTTCGGGACACTCCCCCGACGGGCGCATGGAGGCCCTCGAACTGCCGGATCATCCTTACTTCGTTGGCACACAGTT
CCATCCGGAGTTCAAGTCCCGTCCCGGTGACCCGTCGCCGCCGTTCGTAGGCCTCATCAAGGCTGCAGCAGGTCAAGGGC
CCGATTGA

Upstream 100 bases:

>100_bases
GCGAGAGGCGGACGGGATGGGTGGCGTCCGGTTCCGAGCGGATCGGGAAGTACCGCGCAACCTTATTCTTTCTCGAGTCT
CGGAGCGTGAAGGGGCGCTC

Downstream 100 bases:

>100_bases
ACGCCTCTCGTGGATCACCGTCGGAGAAATGCAGCGCCGCCGCGTTGGGATGTCCACCGGCCTTGGAGCCTTCCAACCAC
CTCGCCATCTTCACCATCAA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MKFVVITGGVVSGIGKGITTASIGRILRARELEVTAVKIDPYINVDAGTMNPFQHGEVFVTEDGVETDLDLGHYERFMDV
TLSGAHNITTGKIYQRVIEKERRGDYLGETVQVIPHITDEIKSWIREVGKASGADVVLVEIGGTVGDIEGMPFYEAVRQL
QLEEGRENVMFVHLTYVPYLEHVHELKTKPTQHSVKELRSLGIQPDAIVCRCERPLDDGVKRKIALHTNVPREAVIDAHD
VDLVYKVPLLLERQGFGDYICERLGLDADEPDYSDWLDFVTRIEEADDEIRIAVVGKYVDLPDAYISIREALVHAGAHVG
VGVDVAWVDSEALEAGDSEAWEEVKDADGVLVPGGFGKRGVEGKIEAVRYARENDVPFLGICLGFQLVVVEYARSVLGLE
DAHSTEFNPDTDHPVVDLLPEQRGVKRKGGTMRLGAEPVVLEEGSLLRRLYDDREIVLERHRHRYEVNPSYVRELEDHGL
RFSGHSPDGRMEALELPDHPYFVGTQFHPEFKSRPGDPSPPFVGLIKAAAGQGPD

Sequences:

>Translated_535_residues
MKFVVITGGVVSGIGKGITTASIGRILRARELEVTAVKIDPYINVDAGTMNPFQHGEVFVTEDGVETDLDLGHYERFMDV
TLSGAHNITTGKIYQRVIEKERRGDYLGETVQVIPHITDEIKSWIREVGKASGADVVLVEIGGTVGDIEGMPFYEAVRQL
QLEEGRENVMFVHLTYVPYLEHVHELKTKPTQHSVKELRSLGIQPDAIVCRCERPLDDGVKRKIALHTNVPREAVIDAHD
VDLVYKVPLLLERQGFGDYICERLGLDADEPDYSDWLDFVTRIEEADDEIRIAVVGKYVDLPDAYISIREALVHAGAHVG
VGVDVAWVDSEALEAGDSEAWEEVKDADGVLVPGGFGKRGVEGKIEAVRYARENDVPFLGICLGFQLVVVEYARSVLGLE
DAHSTEFNPDTDHPVVDLLPEQRGVKRKGGTMRLGAEPVVLEEGSLLRRLYDDREIVLERHRHRYEVNPSYVRELEDHGL
RFSGHSPDGRMEALELPDHPYFVGTQFHPEFKSRPGDPSPPFVGLIKAAAGQGPD
>Mature_535_residues
MKFVVITGGVVSGIGKGITTASIGRILRARELEVTAVKIDPYINVDAGTMNPFQHGEVFVTEDGVETDLDLGHYERFMDV
TLSGAHNITTGKIYQRVIEKERRGDYLGETVQVIPHITDEIKSWIREVGKASGADVVLVEIGGTVGDIEGMPFYEAVRQL
QLEEGRENVMFVHLTYVPYLEHVHELKTKPTQHSVKELRSLGIQPDAIVCRCERPLDDGVKRKIALHTNVPREAVIDAHD
VDLVYKVPLLLERQGFGDYICERLGLDADEPDYSDWLDFVTRIEEADDEIRIAVVGKYVDLPDAYISIREALVHAGAHVG
VGVDVAWVDSEALEAGDSEAWEEVKDADGVLVPGGFGKRGVEGKIEAVRYARENDVPFLGICLGFQLVVVEYARSVLGLE
DAHSTEFNPDTDHPVVDLLPEQRGVKRKGGTMRLGAEPVVLEEGSLLRRLYDDREIVLERHRHRYEVNPSYVRELEDHGL
RFSGHSPDGRMEALELPDHPYFVGTQFHPEFKSRPGDPSPPFVGLIKAAAGQGPD

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=551, Percent_Identity=51.1796733212341, Blast_Score=557, Evalue=1e-159,
Organism=Homo sapiens, GI28559085, Length=551, Percent_Identity=50.6352087114338, Blast_Score=554, Evalue=1e-158,
Organism=Homo sapiens, GI28559083, Length=551, Percent_Identity=50.6352087114338, Blast_Score=554, Evalue=1e-158,
Organism=Homo sapiens, GI221316689, Length=551, Percent_Identity=50.6352087114338, Blast_Score=554, Evalue=1e-158,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=54.6125461254613, Blast_Score=563, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI25148299, Length=605, Percent_Identity=42.6446280991736, Blast_Score=474, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6319432, Length=561, Percent_Identity=49.0196078431373, Blast_Score=535, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6322563, Length=561, Percent_Identity=47.2370766488414, Blast_Score=520, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24664469, Length=548, Percent_Identity=50.7299270072993, Blast_Score=550, Evalue=1e-156,
Organism=Drosophila melanogaster, GI21357815, Length=493, Percent_Identity=50.1014198782961, Blast_Score=473, Evalue=1e-133,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_METKA (Q8TYT7)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613492.1
- ProteinModelPortal:   Q8TYT7
- SMR:   Q8TYT7
- GeneID:   1477508
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0205
- NMPDR:   fig|190192.1.peg.205
- HOGENOM:   HBG597806
- OMA:   NIKIDPY
- ProtClustDB:   PRK05380
- BioCyc:   MKAN190192:MK0205-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59461; Mature: 59461

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 382-382 ACT_SITE 508-508 ACT_SITE 510-510

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVVITGGVVSGIGKGITTASIGRILRARELEVTAVKIDPYINVDAGTMNPFQHGEVFV
CEEEEEECHHHHHHCCCCHHHHHHHHHHHCCEEEEEEEECCEEEECCCCCCCCCCCEEEE
TEDGVETDLDLGHYERFMDVTLSGAHNITTGKIYQRVIEKERRGDYLGETVQVIPHITDE
ECCCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IKSWIREVGKASGADVVLVEIGGTVGDIEGMPFYEAVRQLQLEEGRENVMFVHLTYVPYL
HHHHHHHHHCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHH
EHVHELKTKPTQHSVKELRSLGIQPDAIVCRCERPLDDGVKRKIALHTNVPREAVIDAHD
HHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHEEEEEECCCCHHHHCCCCC
VDLVYKVPLLLERQGFGDYICERLGLDADEPDYSDWLDFVTRIEEADDEIRIAVVGKYVD
CCEEEECCCEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECC
LPDAYISIREALVHAGAHVGVGVDVAWVDSEALEAGDSEAWEEVKDADGVLVPGGFGKRG
CCHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHCCCHHHHHHHHCCCCEEECCCCCCCC
VEGKIEAVRYARENDVPFLGICLGFQLVVVEYARSVLGLEDAHSTEFNPDTDHPVVDLLP
CCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCC
EQRGVKRKGGTMRLGAEPVVLEEGSLLRRLYDDREIVLERHRHRYEVNPSYVRELEDHGL
CCCCCCCCCCEEEECCCCEEECCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCE
RFSGHSPDGRMEALELPDHPYFVGTQFHPEFKSRPGDPSPPFVGLIKAAAGQGPD
EECCCCCCCCEEEEECCCCCEEECCEECCHHHCCCCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MKFVVITGGVVSGIGKGITTASIGRILRARELEVTAVKIDPYINVDAGTMNPFQHGEVFV
CEEEEEECHHHHHHCCCCHHHHHHHHHHHCCEEEEEEEECCEEEECCCCCCCCCCCEEEE
TEDGVETDLDLGHYERFMDVTLSGAHNITTGKIYQRVIEKERRGDYLGETVQVIPHITDE
ECCCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IKSWIREVGKASGADVVLVEIGGTVGDIEGMPFYEAVRQLQLEEGRENVMFVHLTYVPYL
HHHHHHHHHCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHH
EHVHELKTKPTQHSVKELRSLGIQPDAIVCRCERPLDDGVKRKIALHTNVPREAVIDAHD
HHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHEEEEEECCCCHHHHCCCCC
VDLVYKVPLLLERQGFGDYICERLGLDADEPDYSDWLDFVTRIEEADDEIRIAVVGKYVD
CCEEEECCCEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECC
LPDAYISIREALVHAGAHVGVGVDVAWVDSEALEAGDSEAWEEVKDADGVLVPGGFGKRG
CCHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHCCCHHHHHHHHCCCCEEECCCCCCCC
VEGKIEAVRYARENDVPFLGICLGFQLVVVEYARSVLGLEDAHSTEFNPDTDHPVVDLLP
CCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCC
EQRGVKRKGGTMRLGAEPVVLEEGSLLRRLYDDREIVLERHRHRYEVNPSYVRELEDHGL
CCCCCCCCCCEEEECCCCEEECCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCE
RFSGHSPDGRMEALELPDHPYFVGTQFHPEFKSRPGDPSPPFVGLIKAAAGQGPD
EECCCCCCCCEEEEECCCCCEEECCEECCHHHCCCCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014